FAQ
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No. ExoQuick®-TC UltraPure requires no ultracentrifuge, no pre-concentration equipment, and no ultrafiltration system. The entire workflow is completed using standard laboratory benchtop equipment in under 20 minutes of hands-on time per... Read more
Yes. TEM confirms that vesicles retain their native bilayer membrane morphology after isolation. Functional validation using copGFP-loaded EVs from the XPACK530 stable HEK293 cell line demonstrates that engineered EV cargo is fully preserved through the isolation workflow, confirming suitability for cargo delivery and functional... Read more
EVs isolated with ExoQuick®-TC UltraPure are validated for:
Nanoparticle Tracking Analysis (NTA)
Transmission Electron Microscopy (TEM)
Western blot (EV marker detection)
ELISA
RNA extraction, small RNA/miRNA sequencing, and next-generation sequencing (NGS) — compatible with SBI's NGS service
Mass spectrometry proteomics (in-gel digestion)
EV uptake and cargo delivery assays
In vivo / ex vivo administration... Read more
ExoQuick®-TC UltraPure outperforms both SEC (e.g., qEV) and tangential flow filtration (TFF) in yield and purity for cell culture media applications.
vs. SEC (qEV): Delivers more than 50× higher EV yield, with superior removal of albumin, IgG, and lipoproteins. SEC requires pre-concentration of conditioned media before loading — ExoQuick®-TC UltraPure processes your sample directly, no pre-concentration required.
vs. TFF: At equivalent large-volume inputs (100 mL), EQ-TC UltraPure produces significantly cleaner EV preparations — confirmed by Ponceau S staining and Western blot — with higher EV marker expression and lower protein contamination. Unlike TFF, no specialised tangential flow filtration system is... Read more
Yes. ExoQuick®-TC UltraPure is validated for use with both standard tissue culture flask media and large-scale bioreactor harvests. Unlike SEC and TFF, it requires no pre-concentration and no ultrafiltration — making it well-suited for therapeutic EV development and cell therapy production workflows where maintaining yield and purity at scale is... Read more
Yes, with one important distinction. The kit is fully compatible with in-gel digestion workflows (trypsin, pepsin) because PEG — the precipitation reagent — does not migrate into the gel and does not interfere with MS analysis. For in-solution digestion workflows, PEG can cause ion suppression and background signals. We recommend consulting with your proteomics facility or contacting our technical team before proceeding with in-solution digestion. For EV proteomics using precipitation-based methods, we also recommend reviewing our application note on EV precipitation vs SEC for proteomics... Read more
The dual-action workflow removes three major contaminant classes: albumin (BSA), IgG, and lipoproteins. Near-complete lipoprotein depletion is confirmed by ApoB ELISA. Albumin and IgG removal is validated by Western blot and Ponceau S... Read more
The Small Kit (EQ-TC UltraPure_S) processes 10 mL of conditioned media per reaction. The Large Kit (EQ-TC UltraPure_L) processes 100 mL per reaction. Both kits provide 10 reactions. For scale-up beyond 100 mL — including 1 L bioreactor harvests — contact our technical support team for guidance and bulk... Read more
No, according to U.S, Australian, and EU regulations, Exo-Fect does not require an MSDS because it does not contain components above the thresholds for hazardous or carcinogenic substances.
Transfected exosomes can be added to the cells in a culture plate grown in exosome-depleted FBS media. The quantity can be adjusted depending on your experimental requirements.
Exo-Fect has been successfully used to transfect exosomes with small RNAs, longer mRNAs, and plasmid DNA. Cells treated with these transfected exosomes have demonstrated successful uptake and expression of these nucleic... Read more
The transfected exosomes can be added to any target cells, including those used in cell culture, depending on your experimental requirements.
Although only in vitro data has been established and no internal data is available for in vivo experiments, it's possible that the transfected exosomes may be used for in vivo studies.
The kit comes in two sizes, each containing Exo-Fect Reagent, Positive Control siRNA (TX-Red), and ExoQuickTC. The specific quantities differ depending on whether you get the 10 reaction or 20 reaction... Read more
The Exo-Fect transfection protocol takes less than an hour to complete.
Exo-Fect can transfect si/miRNA, mRNA, and even plasmid DNA into isolated exosomes.
Exo-Fect is a novel nucleic acid transfer agent that facilitates the transfection of nucleic acids directly into isolated exosomes. These transfected exosomes can then deliver the nucleic acids into target... Read more
SmartSEC™ HT can be used for various applications, including EV biomarker research, diagnostics, and therapeutics R&D. It can help accelerate your research by enabling high-throughput, reliable, and efficient EV... Read more
Yes, SmartSEC™ HT is specifically designed for EV isolation from serum and plasma samples. You can load the serum or plasma directly onto the SmartSEC HT plate after a brief centrifugation, without the need for any... Read more
SmartSEC™ HT offers several advantages, including high-throughput isolation of EVs from up to 96 samples in less than an hour, better levels of purity and yield compared to ultracentrifugation, and the ability to use all wells in a single run or save wells for later... Read more
SmartSEC™ HT uses size-exclusion chromatography (SEC) technology to isolate EVs from biological samples. The system includes a 96-well plate with porous beads that separate EVs from other components based on... Read more
SmartSEC™ HT is an EV (extracellular vesicle) isolation system in a 96-well format. It is designed for high-throughput, high purity, and high yield EV isolation from serum and plasma.
SmartSEC™ HT can be used to accelerate EV biomarker, diagnostics, and therapeutics R&D with its high-throughput, plate-based EV isolation capabilities.
According to the provided content, SmartSEC™ HT can achieve levels of purity and yield that are better than ultracentrifugation.
SmartSEC™ HT offers several benefits, including its high-throughput capabilities, ability to isolate EVs from as many as 96 samples in less than one hour, flexibility in running a partial plate, and ease of use in loading serum or plasma directly onto the plate without... Read more
SmartSEC™ HT is an EV isolation system in a 96-well format that allows for high-purity, high-yield, high-throughput EV isolation from serum and plasma.
To speak to a specialist, you can either Contact Us through the website or call 1-(888) 689-1126.
To order TransDux™ MAX Lentivirus Transduction Reagent, use the catalog number LV860A-1. The reagent provides 100 transductions.
To use TransDux MAX, follow these steps:
Mix both TransDux and MAX Enhancer reagents into conditioned media from target cells.
Infect the cells with your lentivirus of choice.
Observe higher transduction efficiency compared to other methods.
Is TransDux™ MAX compatible with various cell types and lentivectors?
Yes, TransDux™ MAX works with a wide variety of cell lines and is compatible with all types of packaged lentivirus. It also works well with hard-to-transduce... Read more
TransDux MAX comes in a two-component format (TransDux and MAX Enhancer) good for 100 transduction reactions. Mix both reagents into conditioned media from target cells and infect with your lentivirus of choice for a higher level of transduction... Read more
TransDux™ MAX offers several advantages:
Efficient: up to 8-fold greater transduction efficiency than polybrene and 4-fold greater efficiency than standard TransDux reagent
Easy-to-use: requires minimal hands-on time... Read more
TransDux™ MAX Lentivirus Transduction Reagent is designed to maximize transduction efficiencies for more reliable and reproducible gene delivery, even in infection-resistant cell types.
Yes, the pPACKH1 HIV Lentivector Packaging Kit is available in two sizes: LV500A-1 with 10 transfections and LV510A-1 with 25 transfections. Price is subject to change from year to year.
To speak to a specialist, you can either contact System Biosciences via our website, e-mail us at info@systembio.com, or call our toll-free number at 1-888-266-5066. Read more
The pPACKH1 HIV Lentivector Packaging Kit is based on an HIV-derived system, while the pPACKF1 FIV (Cat. # LV100A-1 and LV100A-5) Lentivector Packaging Kit uses an FIV-derived system. Both systems are designed to effectively leverage the powerful lentiviral transduction platform for gene delivery and expression in a wide range of mammalian... Read more
The pPACKH1 HIV Lentivector Packaging Kit enables you to easily generate high-titer lentiviral preparations by co-transfecting the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production... Read more
The pPACKH1 Packaging Plasmid Mix contains three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudo typed lentiviral particles. Co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs,... Read more
The pPACKH1 HIV Lentivector Packaging Kit can transduce a broad range of cell types, including dividing and quiescent cells, primary cells, stem cells, neuronal cells, endothelial cells, and more.
The pPACKH1 HIV Lentivector Packaging Kit is designed to efficiently package most third-generation lentivectors, allowing for the delivery and expression of lentiviral constructs in a wide range of mammalian... Read more
You can contact a specialist by visiting the Contact Us page or calling 1-(888) 640-4371.
Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use. Commercial entities can inquire about a No-Fee Research Only License... Read more
Yes, using the Excision Only PiggyBac Transposase (Cat. # PB220PA-1), the ITRs and cargo that the Super PiggyBac Transposase integrates into the genome can be removed in a footprint-free way, leaving behind the original genomic sequence and nothing... Read more
The PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. The Super PiggyBac Transposase recognizes transposon-specific inverted terminal repeats (ITRs) and efficiently integrates the ITRs and intervening DNA into the genome at TTAA... Read more
Delivery of virtually any-sized DNA insert, from 10 – 100 kb
Footprint-free removal that leaves no PiggyBac sequences behind
Ability to make transgenic cell lines with a single transfection
Capability to integrate multiple PiggyBac Vectors in a single transfection
Insertion of an expression cassette into human, mouse, and rat cells
Variety of PiggyBac Vectors with different promoters and... Read more
The Super PiggyBac Transposase Expression Vector is an engineered, hyperactive version of the enzyme used in SBI's PiggyBac Transposon System. It can efficiently integrate any-sized DNA insert into the genome, enabling easy and consistent... Read more
The user manual and product sheet for PEG-it Lentivirus Concentration Reagent can be found on the manufacturer's website under the "Resources" section.
PEG-it Virus Precipitation Solution is available in two sizes:
100 mL (Catalog Number: LV810A-1)
250 mL (Catalog Number: LV825A-1)
100 mL (Catalog Number: LV810A-1)
250 mL (Catalog Number: LV825A-1)
To speak to a specialist, you can contact System Biosciences at info@systembio.com or call our toll-free number at 1-(888) 640-4371. Read more
Yes, PEG-it-treated virus is non-toxic to stem cells and results in highly effective transductions.
PEG-it acts as a cryopreservative for concentrated virus, allowing it to last longer in the freezer and survive multiple freeze-thaw cycles with minimal loss of titer.
PEG-it offers several benefits:
Concentrates virus particles 10- to 100-fold, leading to higher infectious units per mL
Eliminates the need for ultracentrifugation
Non-toxic and effective with stem cells
Acts as a cryopreservative, protecting the virus from multiple freeze-thaw cycles with minimal loss of... Read more
PEG-it works by concentrating virus particles in a simple, hands-on procedure. Just add PEG-it to the collected medium, incubate overnight at 4°C, and spin at 1500g for 30 minutes. This process helps achieve higher viral titers by concentrating the... Read more
PEG-it Virus Precipitation Solution is a reagent used to easily concentrate lentivirus particles for ultra-high titers, without the need for ultracentrifugation. It is suitable for concentrating pseudoviral particles from large volumes of medium and acts as a cryopreservative for concentrated... Read more
Yes, there are multiple research studies that have used Exo-FBS. You can find a list of citations on the product information page, which includes a variety of research articles from different scientific... Read more
You can contact a specialist by visiting the Contact Us page or by calling 1-(888) 640-4371.
Exo-FBS can be purchased in two sizes: 250 mL (Catalog Number: EXO-FBS-250A-1) and 50 mL (Catalog Number: EXO-FBS-50A-1). Add the desired quantity to your cart and proceed with the checkout process.
Yes, Exo-FBS has been shown to support comparable cell growth rates as standard FBS. In fact, it has demonstrated more robust cell growth than some competitors' exosome-depleted FBS products.
Exo-FBS is interchangeable with standard FBS. Simply add 10% Exo-FBS to your DMEM or RPMI cell culture media.
Exo-FBS has exosome-sized vesicles removed, very low levels of CD63-positive bovine exosomes, and undetectable levels of bovine miRNAs. This makes it suitable for exosome research, while maintaining comparable growth rates and interchangeability with standard... Read more
Exo-FBS is an exosome-depleted fetal bovine serum (FBS) that has been specifically designed for researchers interested in isolating exosomes from cultured cells without the complications introduced by bovine exosomes present in conventional... Read more
The protocol is quick and easy, requiring only 10 minutes of hands-on time. Simply add ExoQuick-TC ULTRA to 5 mL of tissue culture media or other biofluid, incubate overnight at 4°C, collect EVs by spinning at 3,000g for 10 minutes, resuspend EVs and add to pre-washed ExoQuick-TC ULTRA columns, and spin at 1,000g for 30 seconds to collect the EVs, which are then ready to... Read more
Isolated EVs can be used for a wide range of downstream applications, such as western blotting, mass spectrometry, NGS sequencing, exosome labeling, and in vivo/ex vivo exosome delivery.
You can contact a specialist through the "Contact Us" form on the website or call 1-877-705-6256 .
Yes, ExoQuick-TC® ULTRA offers a more cost-effective solution for each reaction compared to using a competitor's kit.
ExoQuick-TC® ULTRA requires less than 20 minutes of hands-on time, including a convenient column-based format for clean-up.
ExoQuick-TC® ULTRA outperforms other methods, including ultracentrifugation and competitors' kits, by reducing carry-over of albumins by 75% and immunoglobulins by 40%. It also isolates more EVs per normalized input volume and increases the sensitivity of EV biomarker detection by up to 11-fold (CD9) and 8-fold... Read more
ExoQuick-TC® ULTRA is a cutting-edge method for isolating EVs from most biofluids, providing faster and cleaner results compared to other methods. It offers reduced carry-over of albumins and immunoglobulins, higher yields, better biomarker detection, and is... Read more
For more information, contact a specialist by using the Contact Us form on the website or by calling 1-(888) 640-4371. A specialist will reach out to you within 24 hours. Read more
One reaction requires as little as 250 μL of serum or plasma with the ExoQuick® ULTRA kit.
The ExoQuick® ULTRA EV Isolation System is suitable for various downstream applications, including western blotting, mass spectrometry, NGS sequencing, exosome labeling, and in vivo/ex vivo exosome... Read more
ExoQuick® ULTRA delivers higher yields of clean exosomes with a faster and more cost-effective process compared to ultracentrifugation and competitor kits. Additionally, it reduces the carry-over of albumins and immunoglobulins more effectively than other... Read more
The benefits of ExoQuick® ULTRA include:
420X more EVs compared to ultracentrifugation
Reduced carry-over of albumins (75%) and immunoglobulins (40%)
Higher EV yields per normalized input volume
Improved sensitivity of EV biomarker detection (up to 11-fold for CD9 and 8-fold for CD81)
Fast processing with less than 20 minutes of hands-on time
Cost-effective compared to competitors' kits
What is the price of the ExoQuick® ULTRA EV Isolation Kit for Serum and... Read more
The ExoQuick® ULTRA EV Isolation System is an advanced polymer-based method for isolating Extracellular Vesicles (EVs) from serum and plasma (pre-treatment with thrombin recommended), delivering higher yields, cleaner samples, and faster processing times compared to ultracentrifugation and competitors'... Read more
You can verify the presence of exosomes with a number of different methods, including ELISA/Western blotting for general exosome markers (CD63, CD9, CD81, and HSP70) or EXORAY panel, NanoSight analysis, or electron... Read more
To isolate exosomes from tissue culture media, simply add an appropriate volume of ExoQuick-TC, incubate overnight at 4°C, and isolate exosomes with a 30-minute low-speed spin (1500g). Isolated exosomes can be found in the pellet and resuspended in an appropriate... Read more
Yes, ExoQuick-TC is an excellent option for researchers working with low sample volumes, such as clinical research samples or small animal models.
ExoQuick-TC-isolated exosomes can be used for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies (such as in cell-to-cell signaling), and basic biology (such as role in... Read more
ExoQuick-TC can be used to purify exosomes from a wide variety of tissue culture media, and from certain biofluids such as saliva, urine, follicular fluid, and breast milk.
ExoQuick-TC saves time and labor, conserves precious sample, delivers high yields of functional, high-quality exosomes, and is easily scalable. It can be used for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies, and basic... Read more
ExoQuick-TC is a fast and scalable method for isolating exosomes from tissue culture media and certain non-viscous biofluids (saliva, urine, follicular fluid, and breast milk). It is a proprietary polymer that gently precipitates exosomes, providing high yields of functional, high-quality exosomes that are suitable for a wide range of downstream... Read more
Yes, ExoQuick-isolated exosomes can be used for studying exosome-associated nucleic acids like microRNAs, siRNAs, and mRNA. Quantitative analytical techniques like qPCR, microarray studies, and next-generation sequencing are all compatible with nucleic acids isolated from ExoQuick-purified exosomes.
If you need more information or want to speak to a specialist, you can contact the company at 1-(866) 417-9257 or visit our... Read more
Yes, ExoQuick-isolated exosomes can be used for various protein profiling and characterization studies, such as mass spectrometry (in-gel digestion), Western blotting, ELISA, and more.
For serum, plasma, and ascites fluid, use 63 μl of ExoQuick per 250 μl of the biofluid.
ExoQuick gently precipitates exosomes from biofluids. To use ExoQuick, add the appropriate amount of the solution to your cleared biofluid, refrigerate, and centrifuge. The exosomes will be in the pellet, ready for resuspension in an appropriate... Read more
ExoQuick can be used for a wide range of downstream applications, including biomarker studies, exosomal miRNA profiling, exosomal proteomics, exosomal lipidomics/metabolomics, functional studies (such as cell-to-cell signaling), and basic biology (such as roles in... Read more
ExoQuick is a proprietary polymer that enables fast, high-throughput, and quantitative isolation of exosomes from low volumes of viscous biofluids, such as serum, plasma, or ascites fluid. It is a proven alternative to ultracentrifugation, saving time and labor while conserving precious... Read more
You can contact a specialist by visiting the "Contact Us" page on the website or by calling 1-(888) 689-1126.
Yes, the SmartSEC™ Mini EV Isolation System has been validated for samples from Drosophila, Planaria, Arabidopsis, human, mouse, and rat.
The SmartSEC Mini workflow is simple. Pre-wash the SmartSEC Mini column, load your sample, incubate at room temperature for 30 minutes, and centrifuge for 30 seconds at 500 x g to recover isolated EVs. To maximize sample recovery, add more buffer to the column and collect up to two additional... Read more
Yes, the SmartSEC™ Mini EV Isolation System is compatible with most downstream applications, including mass spectrometry, RNA sequencing (NGS), Western blotting, nanoparticle tracking analysis (NTA), and transmission electron microscopy... Read more
The SmartSEC™ Mini EV Isolation System delivers better purity and yield than ultracentrifugation. It is also faster, easier to use, and requires lower sample volumes.
The SmartSEC™ Mini EV Isolation System is suitable for isolating EVs from a range of biofluids, including hemolymph from Drosophila melanogaster, cell culture from Planaria, apoplastic fluid from Arabidopsis Thaliana, and serum or plasma from human, mouse, and... Read more
The SmartSEC™ Mini EV Isolation System is a kit that uses powerful SmartSEC™ technology to isolate extracellular vesicles (EVs) from small volumes of biofluids, such as serum and developmental biology samples. It is the only commercially available EV isolation kit for sample volumes as low as 10... Read more
To speak to a specialist about SmartSEC Single, you can contact SBI TechSupport at tech@systembio.com, or call 1-866-956-6608. Read more
SmartSEC Single is validated for use with human serum, plasma, and CSF, as well as Aplysia californica hemolymph.
SmartSEC Single is compatible with most downstream applications, including mass spectrometry, western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and RNA-sequencing... Read more
Each SmartSEC Single kit comes with sufficient individual SmartSEC columns and buffer to process 10 samples.
SmartSEC Single provides better purity and yield than ultracentrifugation and higher EV concentration per fraction than competitors' conventional size exclusion chromatography kits. It is also validated for use with a variety of biofluids and compatible with a wide range of downstream applications. Protocol is easy and... Read more
SmartSEC Single uses size exclusion chromatography (SEC) to separate EVs from other contaminants. It also includes a contaminant trapping feature that enhances the capabilities of conventional SEC. Simply apply 100 – 250 µL of cleared serum or plasma with additional column buffer or up to 4 mL of other biofluids directly to the pre-washed column, incubate, and centrifuge to elute the EVs. You may collect 1 to 2 additional fractions (if desired) by adding more buffer to the... Read more
SmartSEC Single provides a higher EV concentration in single fraction than competitors' conventional size exclusion chromatography kits, and it is validated for use with human serum, plasma, and CSF as well as Aplysia californica hemolymph. It is also compatible with most downstream applications, including mass spectrometry, western blotting, nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and RNA-sequencing (NGS). No need to choose between yield and purity when one can have both with... Read more
SmartSEC Single is a size exclusion-based technology designed for isolating extracellular vesicles (EVs) in a single tube format. It offers a fast, easy-to-use approach and incorporates proprietary contaminant trapping technology, resulting in higher purity and yield compared to... Read more
