SmartSEC Single uses size exclusion chromatography (SEC) to separate EVs from other contaminants. It also includes a contaminant trapping feature that enhances the capabilities of conventional SEC. Simply apply 100 – 250 µL of cleared serum or plasma with additional column buffer or up to 4 mL of other biofluids directly to the pre-washed column, incubate, and centrifuge to elute the EVs. You may collect 1 to 2 additional fractions (if desired) by adding more buffer to the column.