PiggyBac Transposon

Easy, consistent transgenesis free from limitations on insert size and available in a footprint-free format

For transgenesis that’s easy, consistent, and not limited by cargo size, SBI’s PiggyBac Transposon System is an excellent choice. The system consists of a PiggyBac Vector and the Super PiggyBac Transposase which recognizes transposon-specific inverted terminal repeats (ITRs) and efficiently integrates the ITRs and intervening DNA into the genome at TTAA sites. The Super PiggyBac Transposase is delivered to the cell via the Super PiggyBac Transposase Expression Vector, which is co-transfected with one or more PiggyBac Vectors.

In addition to ease-of-use, consistency, and the lack of limits on DNA insert size, what sets this system apart is the ability to reverse the integration reaction in a footprint-free way—with the Excision Only PiggyBac Transposase (Cat.# PB220PA-1), the ITRs and cargo that the Super PiggyBac Transposase integrates into the genome can be removed, leaving behind the original genomic sequence and nothing else.

PiggyBac Transposon Products

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PiggyBac Gene Editing HR Targeting Vector (MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2)

Without a trace—make seamless gene edits with no residual footprint—includes dual GFP/puromycin selection and on-target enrichment with TK selection
Size:10 µg (Cat. # PBHR100A-1)

Super PiggyBac Transposase Expression Vector

Catalyze your PiggyBac genome engineering with this enhanced, hyperactive transposase that can insert any-length DNA segment into the genome
Size:10 µg (Cat. # PB210PA-1)

Excision-only PiggyBac Transposase Expression Vector

Remove a PiggyBac insert from the genome and restore the original genomic sequence with this Excision-only PiggyBac Transposase
Size:10 µg (Cat. # PB220PA-1)

Cumate Solution for the PiggyBac Transposon System

Control the cumate-inducible promoter used on SBI’s PiggyBac Vectors with this ready-to-add 10,000x inducer solution
Size:500 µL (Cat. # PBQM100A-1)

PB-Cuo-MCS-IRES-GFP-EF1α-CymR-Puro Inducible cDNA Cloning and Expression Vector

Combining easy PiggyBac transgenesis with our robust and titratable cumate-inducible expression system, this PiggyBac Vector is designed for cDNA expression
Size:10 µg (Cat. # PBQM812A-1)

PB-Cuo-miR-302/367-IRES-GFP-EF1α-CymR-Puro Inducible Human iPSC Vector

Take advantage of the easy transgenesis possible with PiggyBac to deliver the miR-302/367 cluster for iPSC generation under cumate-inducible control
Size:10 µg (Cat. # PBQM-MIR302)

PB-Cuo-shMCS-IRES-GFP-EF1α-CymR-Puro Inducible shRNA Cloning and Expression Vector

Combining easy PiggyBac transgenesis with our robust and titratable cumate-inducible expression system, this PiggyBac Vector is designed for shRNA expression
Size:10 µg (Cat. # PBQMSH812A-1)

PiggyBac qPCR Copy Number Kit

Find out how many copies of your PiggyBac insert have been integrated into the genome with this accurate, qPCR-based kit
Size:20 Reactions (Cat. # PBC100A-1)

PB-EF1α-Oct4-Sox2-Klf4-Myc-IRES-GFP Human 4-in-1 iPSC PiggyBac Vector

Take advantage of the large insert capabilities of the PiggyBac Transposon System to simultaneously deliver four human reprogramming factors for iPSC generation
Size:10 µg (Cat. # PB630A-1)

PB-CMV-miR-302/367-EF1α-GFP iPSC PiggyBac Vector

Take advantage of the easy transgenesis possible with PiggyBac to deliver and constitutively express the miR-302/367 cluster for iPSC generation
Size:10 µg (Cat. # PB-MIR302)

PB-SV40-Neo-EF1α-GFP-CAGs-cMyc-Klf4-Oct4-Sox2 Mouse 4-in-1 iPSC Vector

Take advantage of the large insert capabilities of the PiggyBac Transposon System to simultaneously deliver four mouse reprogramming factors for iPSC generation
Size:10 µg (Cat. # PB400A-1)

PB-CMV-MCS-EF1α-GFP PiggyBac cDNA Cloning and Expression Vector

Easily deliver your gene-of-interest using PiggyBac – this vector includes a GFP reporter gene and drives your cDNA with the strong CMV promoter
Size:10 µg (Cat. # PB511B-1)

PB-CMV-MCS-EF1α-RFP PiggyBac cDNA Cloning and Expression Vector

Easily deliver your gene-of-interest using PiggyBac – this vector includes an RFP reporter gene and drives your cDNA with the strong CMV promoter
Size:10 µg (Cat. # PB512B-1)

PB-CMV-MCS-EF1α-GreenPuro PiggyBac cDNA Cloning and Expression Vector

Easily deliver your gene-of-interest using PiggyBac – this vector includes both GFP and puro reporters and drives your cDNA with the strong CMV promoter
Size:10 µg (Cat. # PB513B-1)

PB-CMV-MCS-EF1α-RedPuro PiggyBac cDNA Cloning and Expression Vector

Easily deliver your gene-of-interest using PiggyBac – this vector includes both RFP and puro reporters and drives your cDNA with the strong CMV promoter
Size:10 µg (Cat. # PB514B-2)

PB-EF1α-MCS-IRES-GFP PiggyBac cDNA Cloning and Expression Vector

Easily deliver your gene-of-interest using PiggyBac – this vector uses the EF1α promoter to co-expresses your cDNA and a GFP reporter via an IRES element
Size:10 µg (Cat. # PB530A-2)

PB-EF1α-MCS-IRES-RFP PiggyBac cDNA Cloning and Expression Vector

Easily deliver your gene-of-interest using PiggyBac – this vector uses the EF1α promoter to co-expresses your cDNA and an RFP reporter via an IRES element
Size:10 µg (Cat. # PB531A-2)

PB-EF1α-MCS-IRES-Neo PiggyBac cDNA Cloning and Expression Vector

Easily deliver your gene-of-interest using PiggyBac – this vector uses the EF1α promoter to co-expresses your cDNA and a neomycin marker via an IRES element
Size:10 µg (Cat. # PB533A-2)

PB-MSCV-MCS-EF1α-GreenPuro PiggyBac cDNA Cloning and Expression Vector

Deliver and express your gene-of-interest in hematopoietic and stem cells using PiggyBac – this vector includes GFP and drives your cDNA with the MSCV promoter
Size:10 µg (Cat. # PB713B-1)

PB-CMV-MCS-EF1α-Puro PiggyBac cDNA Cloning and Expression Vector

Easily deliver your gene-of-interest using PiggyBac – this vector includes a puromycin resistance gene and drives your cDNA with the strong CMV promoter
Size:10 µg (Cat. # PB510B-1)