Minicircle Technology

Get sustained, episomal gene expression free from foreign DNA—great for model animal generation and gene therapy research

Produced as small excised, circular DNA fragments from a parental plasmid, the non-viral, episomal Minicircle expression cassette is free of any bacterial plasmid DNA sequences, and comes with a variety of promoter and reporter combinations. Their small size facilitates more efficient transfection than what’s possible with standard-sized plasmids, and, while Minicircles do not replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue for months in non-dividing cells.

Minicircle Technology

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Arabinose Induction Solution

Induce minicircle production and removal of the vector backbone with this ready-to-add Arabinose Solution—use with our minicircle production strain ZYCY10P3S2T
Size:20 mL (Cat. # MN850A-1)

ZYCY10P3S2T Minicircle Production Strain (Competent Cells)

Produce minicircles from SBI’s Parental Vectors with the ZYCY10P3S2T Minicircle Production Strain – for-profit customers, please see Product Note (in Overview)
Size:5 Vials (Cat. # MN900A-1)

MC-EasyTM Minicircle DNA Production Kit (without Competent Cells)

Simplify minicircle production with this kit that provides an easy and efficient way to produce high-quality minicircle DNA.
Sizes:5 Preps (Cat. # MN910A-1), 10 Preps (Cat. # MN915A-1)

MC-EasyTM Minicircle DNA Production Kit (with Competent Cells)

Easy minicircle production with this kit that provides an efficient way to produce high-quality minicircle DNA (includes competent ZYCY10P3S2T cells).
Sizes:5 Preps (Cat. # MN920A-1), 10 Preps (Cat. # MN925A-1)

MC-EasyTM Bonus Media Pack

Extra media to supplement SBI’s MC-Easy Minicircle Production Kit – includes both Growth and Induction media.
Size:5 Preps (Cat. # MN950A-1)

Kanamycin Solution

Select for SBI’s parental minicircle vectors during cloning and minicircle production with this ready-to-add Kanamycin Solution
Size:20 mL (Cat. # MN800A-1)

pMC.CMV-GFP-SV40PolyA Parental Minicircle Positive Control Plasmid Vector

Easily implement positive controls for your minicircle projects with pMC.CMV-GFP-SV40PolyA, a Parental Minicircle Positive Control Plasmid
Size:10 µg (Cat. # MN601A-1)

pMC.CMV-GFP-SV40PolyA Pre-made Positive Control Minicircle

Easily implement positive controls for your minicircle projects with pMC.CMV-GFP-SV40PolyA, a ready-to-transfect, pre-made, positive control minicircle
Size:40 µg (Cat. # MN601MC-1)

pMC.CMV-RFP-SV40PolyA Parental Minicircle Positive Control Plasmid Vector

Easily implement positive controls for your minicircle projects with pMC.CMV-RFP-SV40PolyA, a Parental Minicircle Positive Control Plasmid
Size:10 µg (Cat. # MN602A-1)

pMC.CMV-RFP-SV40PolyA Pre-made Positive Control Minicircle

Easily implement positive controls for your minicircle projects with pMC.CMV-RFP-SV40PolyA, a ready-to-transfect, pre-made, positive control minicircle
Size:40 µg (Cat. # MN602MC-1)

pMC.BESPX-MCS1 Empty Parental Minicircle Cloning Vector

Get maximum flexibility with pMC.BESPX-MCS1, a Parental Minicircle Cloning Vector with only an MCS (MCS1) for episomal expression free of foreign DNA
Size:10 µg (Cat. # MN100A-1)

pMC.BESPX-MCS2 Empty Parental Minicircle Cloning Vector

Get maximum flexibility with pMC.BESPX-MCS2, a Parental Minicircle Cloning Vector with only an MCS (MCS2) for episomal expression free of foreign DNA
Size:10 µg (Cat. # MN100B-1)

pMC.CMV-MCS-SV40polyA Parental Minicircle Cloning Vector

Get the advantages of Minicircle Technology with pMC.CMV-MCS-SV40polyA, a Parental Minicircle Cloning Vector for episomal expression free of foreign DNA
Size:10 µg (Cat. # MN501A-1)

pMC.EF1α-MCS-SV40polyA Parental Minicircle Cloning Vector

Get the advantages of Minicircle Technology with pMC.EF1α-MCS-SV40polyA, a Parental Minicircle Cloning Vector for episomal expression free of foreign DNA
Size:10 µg (Cat. # MN502A-1)

pMC.CMV-MCS-EF1α-GFP-SV40polyA Parental Minicircle Cloning Vector

Take advantage of Minicircle Technology with pMC.CMV-MCS-EF1α-GFP-SV40polyA, a Parental Minicircle Cloning Vector for episomal expression free of foreign DNA
Size:10 µg (Cat. # MN511A-1)

pMC.CMV-MCS-EF1α-RFP-SV40polyA Parental Minicircle Cloning Vector

Take advantage of Minicircle Technology with pMC.CMV-MCS-EF1α-RFP-SV40polyA, a Parental Minicircle Cloning Vector for episomal expression free of foreign DNA
Size:10 µg (Cat. # MN512A-1)

pMC.EF1α-MCS-IRES-GFP-SV40polyA Parental Minicircle Cloning Vector

Take advantage of Minicircle Technology with pMC.EF1α-MCS-IRES-GFP-SV40polyA, a Parental Minicircle Cloning Vector for episomal expression free of foreign DNA
Size:10 µg (Cat. # MN530A-1)

pMC.EF1α-MCS-IRES-RFP-SV40polyA Parental Minicircle Cloning Vector

Take advantage of Minicircle Technology with pMC.EF1α-MCS-IRES-RFP-SV40polyA, a Parental Minicircle Cloning Vector for episomal expression free of foreign DNA
Size:10 µg (Cat. # MN531A-1)

Mouse IL-23 Pre-made Minicircle DNA (RSV->FLAG-mP40-mP19-pA)

Study mouse models of inflammation with this ready-to-transfect Mouse IL-23 Pre-made RSV->FLAG-mP40-mP19-pA Minicircle DNA
Size:100 µg (Cat. # MN651MC-1)

pMC.CMV-GFP-T2A-Puro-H1-Scramble Parental Minicircle shRNA Scramble Control Plasmid

Easily implement controls for your shRNA studies with this Parental Minicircle Control Plasmid, which expresses a scrambled shRNA delivered via minicircle
Size:10 µg (Cat. # MNSI000A-1)