Custom Overexpression Stable Cell Lines

Reliable, high-quality custom stable cell line generation for overexpression of your protein- or regulatory RNA-of-interest

Overview

Leverage SBI’s viral and non-viral vectors in robust overexpression cell lines

Whether you want constitutive or inducible overexpression of a protein or regulatory RNA, SBI’s services team can create the construct and the cell line. We have access to a range of technologies—including stably integrating viral and non-viral vectors—and an expert team experienced at working with a variety of cell lines, including adherent and suspension cells. The result is hundreds of successfully constructed stable cell lines for highly satisfied customers.

Why choose SBI’s Stable Cell Line Services?

  • Save time by having SBI build your custom cell lines
  • Leverage our highly-regarded gene expression and delivery technologies
  • Accomplish more with our fast turnaround times and end-to-end services
  • Turn to the company that other companies turn to—we are a trusted stable cell line manufacturer for many large pharma/biotech companies
  • Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Available gene expression and delivery technologies

Viral Systems

  • Lentivectors—high titer and reliable
    • Random integration
    • Limited insert size
    • High copy number
  • Lentivirus Packaging—reliable gene delivery
    • High and ultra-high titers
    • Efficient transduction
    • Optimized reagents

Non-viral Systems

  • PhiC31 Integrase System—one-step single-copy integration
    • Site-specific integration
    • Unlimited insert size
    • Single copy number
  • PinPoint Targeted Integration System—excellent for creating isogenic cell lines
    • Site-specific integration
    • Unlimited insert size
    • Single copy number

Not sure what you need or where to begin? Contact the services team to set up a complimentary phone consultation—email services@systembio.com.

How It Works

Ordering Stable Cell Line Services is easy

Step 1. Contact the services team for a quote by emailing services@systembio.com.

Step 2. Send us any necessary materials such as parent cell lines (optional).

Step 3. Receive your stable cell line (turn-around time depends on the complexity of your project).

SBI’s Stable Cell Line Services are a great way to leverage our highly-regarded lentivector, PhiC31 Integrase, and PinPoint Targeted Integration technologies.

Supporting Data

Successful generation of stable cell lines at SBI

Example of data generated using cell lines constructed by SBI

Example of data generated using cell lines constructed by SBI. (Top row) Stable cell line expressing two genes-of-interest with GFP, RFP, hygromycin, and puromycin selection markers. H2286 adherent cells were grown under puromycin and hygromycin selection. (Bottom row) Stable cell line expressing two separate genes-of-interest with GFP, RFP, hygromycin, and puromycin selection markers. H929 suspension cells were grown under puromycin and hygromycin selection.