Cas9 & gRNA Delivery

Find the best Cas9 and gRNA delivery options for your genome engineering application—choose from mRNA, lentivectors, protein, AAV, and more

With a wealth of options for delivering Cas9 and gRNA, SBI supports a full range of genome engineering applications. Deliver Cas9 and gRNA via mRNA, lentivector, or AAV, or use purified Cas9 protein, and then choose from our all-in-one systems that deliver Cas9 and gRNA on a single vector or two-component systems for separate introduction of Cas9 and gRNA.

Not sure which system to choose? Take a look at our table below, or contact technical support—we’re here to help you find the right product for your application and can provide expert experimental support.

Cas9 & gRNA Delivery


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Cas9 & gRNA Delivery Products

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EF1α-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses RF-EF1α-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS751R-1)

MSCV-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the MSCV promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS960A-1)

MSCV-hspCas9-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNucleaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and streamline genome editing in hard-to-transfect-cells—MSCV-hspCas9-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV320PA-1)

EF1α-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Clone your own gRNA into this vector and simplify delivery of hspCas9 and gRNA.
Size:10 Reactions (Cat. # CAS700A-1)

CAG-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Use the CAG-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase plasmid when you want to reduce off-target events and have transfectable cells.
Size:10 Reactions (Cat. # CAS770A-1)

PGK-hspCas9-H1-gRNA All-in-one Cas9 SmartNuclease Plasmid (circular)

SBI’s first generation Cas9 vector places hspCas9 under the control of the PGK promoter and is now available as a circular plasmid
Size:10 µg (Cat. # CAS980A-1)

CMV-hspCas9(D10A)-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNickaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and enhanced target specificity with Cas9 nickase - CMV-hspCas9(D10A)-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV400PA-1)

EF1α-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNuclease Plasmid that delivers multiple gRNAs—kit bundles EF1α-T7-hspCas9-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS700A-KIT)

CAG-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNickase Plasmid that delivers multiple gRNAs—kit bundles CAG-T7-hspCas9-Nickase-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS770A-KIT)

MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA All-in-one Cas9 SmartNickaseTM Lentivector

Get the convenience of all-in-one Cas9 & gRNA delivery in a lentivector and enhanced target specificity with Cas9 nickase - MSCV-hspCas9(D10A)-T2A-Puro-H1-gRNA.
Size:10 Reactions (Cat. # CASLV420PA-1)

EF1α-T7-hspCas9-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses GFP - EF1α-T7-hspCas9-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS700G-1)

CAG-T7-hspCas9-Nickase-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses GFP—CAG-T7-hspCas9-Nickase-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS770G-1)

EF1α-Blasticidin-H1-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System—EF1α-Blasticidin-H1-gRNA.
Size:10 Reactions (Cat. # CASLV500PA-B)

EF1α-T7-hspCas9-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses RFP- EF1α-T7-hspCas9-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS701R-1)

CAG-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses RFP- CAG-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS771R-1)

EF1α-hsaCas9-U6-gRNA(SA) All-in-one AAV Cas9 SmartNucleaseTM Plasmid

Extend genome editing capabilities to cutting edge in vivo applications with our All-in-one AAV Cas9 SmartNuclease Plasmid EF1α-hsaCas9-U6-gRNA(SA).
Size:10 Reactions (Cat. # CASAAV100PA-1)

EF1α-GFP-H1-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System—EF1α-GFP-H1-gRNA.
Size:10 Reactions (Cat. # CASLV501PA-G)

CAG-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Clone your own gRNA into this vector and simplify delivery of hspCas9 and gRNA.
Size:10 Reactions (Cat. # CAS720A-1)

CMV-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Use the CMV-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickase plasmid when you want to reduce off-target events and have transfectable cells.
Size:10 Reactions (Cat. # CAS790A-1)

EF1α-hsaCas9 Two Vector AAV Cas9 SmartNucleaseTM Expression Plasmid

Extend genome editing capabilities to cutting edge in vivo applications with our Two Vector AAV Cas9 SmartNuclease Expression Plasmid EF1α-hsaCas9.
Size:10 µg (Cat. # CASAAV200PA-1)

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