Cas9 & gRNA Delivery

Find the best Cas9 and gRNA delivery options for your genome engineering application—choose from mRNA, lentivectors, protein, AAV, and more

With a wealth of options for delivering Cas9 and gRNA, SBI supports a full range of genome engineering applications. Deliver Cas9 and gRNA via mRNA, lentivector, or AAV, or use purified Cas9 protein, and then choose from our all-in-one systems that deliver Cas9 and gRNA on a single vector or two-component systems for separate introduction of Cas9 and gRNA.

Not sure which system to choose? Take a look at our table below, or contact technical support—we’re here to help you find the right product for your application and can provide expert experimental support.

Cas9 & gRNA Delivery


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Cas9 & gRNA Delivery Products

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CAG-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNuclease Plasmid that delivers multiple gRNAs – kit bundles CAG-T7-hspCas9-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS720A-KIT)

CMV-T7-hspCas9-Nickase-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNickase Plasmid that delivers multiple gRNAs—kit bundles CMV-T7-hspCas9-Nickase-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS790A-KIT)

EF1α-RFP-U6-gRNA(SA) Two Vector AAV Cas9 SmartNucleaseTM gRNA Expression Plasmid

Extend genome editing capabilities to cutting edge in vivo applications with our Two Vector AAV Cas9 SmartNuclease gRNA Expression Plasmid EF1α-RFP-U6-gRNA(SA).
Size:10 Reactions (Cat. # CASAAV300PA-1)

EF1α-Blasticidin-U6-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System - EF1α-Blasticidin-U6-gRNA.
Size:10 Reactions (Cat. # CASLV510PA-B)

CAG-T7-hspCas9-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses GFP—CAG-T7-hspCas9-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS720G-1)

CMV-T7-hspCas9-Nickase-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses GFP—CMV-T7-hspCas9-Nickase-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS790G-1)

CMV-hspCas9-T2A-Puro Two Vector Cas9 SmartNucleaseTM Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with CMV and has Puro selection.
Sizes:10 µg (Cat. # CASLV100PA-1), 1 Kit (Cat. # CASLV100PA-KIT), 2 x 25 µL (Cat. # CASLV100VA-1)

EF1α-GFP-U6-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System—EF1α-GFP-U6-gRNA.
Size:10 Reactions (Cat. # CASLV511PA-G)

CAG-T7-hspCas9-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses RFP-CAG-T7-hspCas9-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS721R-1)

CMV-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA All-in-one Cas9 SmartNickaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNickase Plasmid that coordinately expresses RFP-CMV-T7-hspCas9-Nickase-T2A-RFP-H1-gRNA
Size:10 Reactions (Cat. # CAS791R-1)

CMV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNucleaseTM Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with CMV and has a GFP marker.
Sizes:10 µg (Cat. # CASLV105PA-1), 1 Kit (Cat. # CASLV105PA-KIT), 2 x 25 µL (Cat. # CASLV105VA-1)

EF1α-RFP-U6-gRNA Two Vector Cas9 SmartNucleaseTM gRNA Expression Lentivector

Efficient lentivector delivery of gRNA for our Two Vector Cas9 SmartNuclease System—EF1α-RFP-U6-gRNA.
Size:10 Reactions (Cat. # CASLV512PA-R)

CMV-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Clone your own gRNA into this vector and simplify delivery of hspCas9 and gRNA.
Size:10 Reactions (Cat. # CAS740A-1)

EF1α-hspCas9-nickase-H1-gRNA All-in-one Cas9 SmartNickase Plasmid (circular)

Use this first-generation SBI EF1α-hspCas9 SmartNickase when you want to reduce off-target events, have transfectable cells, and prefer an intact vector.
Size:10 µg (Cat. # CAS800A-1)

MSCV-hspCas9-T2A-Puro Two Vector Cas9 SmartNucleaseTM Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with MSCV and has Puro selection.
Sizes:10 µg (Cat. # CASLV120PA-1), 1 Kit (Cat. # CASLV120PA-KIT), 2 x 25 µL (Cat. # CASLV120VA-1)

CMV-hspCas9-T2A-Puro-H1-AAVS1_gRNA All-in-one Cas9 SmartNucleaseTM & AAVS1 gRNA Lentivector

Streamline genome editing at the AAVS1 Safe Harbor Site in hard-to-transfect cells with a lentivector that expresses both Cas9 and an AAVS1-targeting gRNA
Sizes:10 µg (Cat. # CASLV601PA-1), 2 x 25 µL (Cat. # CASLV601VA-1)

CMV-T7-hspCas9-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid & Multiplex gRNA Cloning Kit

Easily create an All-in-one Cas9 SmartNuclease Plasmid that delivers multiple gRNAs—kit bundles CMV-T7-hspCas9-H1-gRNA with the Multiplex gRNA Cloning Kit
Size:10 Reactions (Cat. # CAS740A-KIT)

EF1α-hspCas9-DM-H1-gRNA All-in-one Cas9 Null Nuclease Plasmid (circular)

Use the EF1α-hspCas9-DM-H1-gRNA All-in-one Cas9 Null Nuclease Plasmid as a DNA cleavage/DNA nicking-deficient control for your studies.
Size:10 µg (Cat. # CAS805A-1)

MSCV-hspCas9-EF1α-GFP Two Vector Cas9 SmartNucleaseTM Lentivector

When working with difficult-to-transfect-cells, use our lentiviral Cas9 SmartNuclease System. This vector drives Cas9 expression with MSCV and has a GFP marker.
Sizes:10 µg (Cat. # CASLV125PA-1), 1 Kit (Cat. # CASLV125PA-KIT), 2 x 25 µL (Cat. # CASLV125VA-1)

CMV-T7-hspCas9-T2A-GFP-H1-gRNA All-in-one Cas9 SmartNucleaseTM Plasmid

Easily verify transfection efficiencies with this All-in-one Cas9 SmartNuclease Plasmid that coordinately expresses GFP- CMV-T7-hspCas9-T2A-GFP-H1-gRNA
Size:10 Reactions (Cat. # CAS740G-1)

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