AAVS1 Safe Harbor Targeting

Leverage the power of the AAVS1 Safe Harbor Site for robust gene expression with these HR donor vectors already targeted to the AAVS1 site

With HR donor vectors that already contain AAVS1 homology arms, these products streamline genome engineering at the AAVS1 Safe Harbor Site. Create isogenic cell lines, reporter cell lines, Cas9-expressing cell lines, and more, and take advantage of the consistent, robust transgene expression possible at the AAVS1 site.

AAVS1 Safe Harbor Targeting Products

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CMV-hspCas9-T2A-Puro-H1-AAVS1_gRNA All-in-one Cas9 SmartNucleaseTM & AAVS1 gRNA Lentivector

Streamline genome editing at the AAVS1 Safe Harbor Site in hard-to-transfect cells with a lentivector that expresses both Cas9 and an AAVS1-targeting gRNA
Sizes:10 µg (Cat. # CASLV601PA-1), 2 x 25 µL (Cat. # CASLV601VA-1)

All-in-one Cas9 SmartNucleaseTM & AAVS1 gRNA Plasmid

Streamline genome editing at the AAVS1 Safe Harbor Site with a plasmid that expresses both Cas9 and a gRNA that targets the AAVS1 site
Size:10 µg (Cat. # CAS601A-1)

DIY Cas9-expressing Cells Complete Kit

Streamline gRNA screening, functional validation and more—get everything you need to make your own cell lines expressing Cas9 from the AAVS1 site.
Size:1 Kit (Cat. # CAS620A-KIT)

DIY Cas9-expressing Cells HR Donor

Streamline gRNA screening, functional validation and more—make your own Cas9-expressing cell lines with this HR donor that knocks-in Cas9 to the AAVS1 site.
Size:10 µg (Cat. # CAS620A-1)

pAAVS1D-PGK-MCS-EF1α-copGFPpuro PrecisionXTM HR Targeting Vectors

Knock-in a gene- or miRNA-of-interest into the AAVS1 site with our first-generation AAVS1 HR Targeting Vector—pAAVS1D-PGK-MCS-EF1α-copGFPpuro
Size:10 µg (Cat. # GE602A-1)

pAAVS1D-CMV-RFP-EF1α-copGFPpuro PrecisionXTM HR Targeting Vector

Knock-in RFP into the AAVS1 site with our first-generation positive control AAVS1 HR Targeting Vector—pAAVS1D-CMV-RFP-EF1α-copGFPpuro
Size:10 µg (Cat. # GE603A-1)

AAVS1 Safe Harbor Site Targeting 2.0 Complete Kit with All-purpose HR Donor Vector 2.0 (AAVS1-SA-puro-MCS)

Easily knock-in any gene or promoter-gene combination into the AAVS1 Site—includes HR Donor, All-in-one Cas9 & AAVS1 gRNA Vector, and verification primer mix
Size:1 Kit (Cat. # GE620A-KIT)

AAVS1 Safe Harbor Site Targeting Knock-in HR Donor Vector 2.0 (AAVS1-SA-puro-EF1α-MCS)

Easily knock-in any gene into the AAVS1 Safe Harbor Site—your gene-of-interest is driven by the constitutive EF1α promoter
Size:10 µg (Cat. # GE622A-1)

AAVS1 Safe Harbor Site Targeting 2.0 Complete Kit with All-purpose HR Donor Vector 2.0 (AAVS1-SA-puro-EF1α-MCS)

Easily knock-in and constitutively express any gene from the AAVS1 Site—includes HR Donor, All-in-one Cas9 & AAVS1 gRNA Vector, and verification primer mix
Size:1 Kit (Cat. # GE622A-KIT)

AAVS1 Safe Harbor Site Targeting Reporter Knock-in HR Donor Vector 2.0 (AAVS1-SA-puro-MCS-GFP)

Easily create reporter cell lines that express GFP from your promoter at the AAVS1 Safe Harbor Site—clone your promoter into the MCS upstream of GFP
Size:10 µg (Cat. # GE624A-1)

AAVS1 Safe Harbor Site Targeting 2.0 Complete Kit with All-purpose HR Donor Vector 2.0 (AAVS1-SA-puro-MCS-GFP)

Easily create reporter cell lines that express GFP from the AAVS1 Site—includes HR Donor, All-in-one Cas9 & AAVS1 gRNA Vector, and verification primer mix
Size:1 Kit (Cat. # GE624A-KIT)

AAVS1 Safe Harbor Site Targeting All-purpose HR Donor Vector 2.0 (AAVS1-SA-puro-MCS)

Easily knock-in any gene or promoter-gene combination into the AAVS1 Safe Harbor Site
Size:10 µg (Cat. # GE620A-1)

Primer Mix to Confirm Knock-in of AAVS1 Positive Control Donor

Confirm successful knock-in of positive control AAVS1 HR Targeting Vector pAAVS1D-CMV-RFP-EF1α-copGFPpuro with these 5’ and 3’ primers for junction PCR assays
Size:100 µL (Cat. # GE603PR-1)

Primer Mix to Confirm AAVS1 Integration

Easily confirm integration at the AAVS1 site with this 5’ and 3’ primer mix for Junction PCR Assays.
Size:50 Reactions (Cat. # GE640PR-1)