Integrase-based Systems

When you want non-viral expression from low or single-copy integration events, and/or have a large insert size, choose one of SBI’s integrase-based systems

Choose one of SBI’s integrase-based gene expression systems when you want a non-viral system that integrates at low- or single-copy. Both our PhiC31 and PinPoint Systems support unlimited insert sizes, and come in a variety of promoter and reporter combinations.

The PhiC31 Integrase System

  • Frequently used for gene therapy development and related applications
  • Non-viral transgene delivery
  • Single-step process
  • Single-copy integration
  • Preferential integration at active sites
  • Delivery of inserts with no size constraints
  • Easy generation of cell lines

The PinPoint Targeted Integration System

  • Isogenic cell line creation
  • Reliable and efficient cell line generation and retargeting
  • Non-viral transgene delivery
  • Two-step process
  • High-efficiency, site-specific, single-copy integration
  • Delivery of inserts with no size constraints
  • Compatible with high-throughput workflows

Integrase-based Systems

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pCMV-CRE, Cre Recombinase Expression Plasmid

Transiently express Cre recombinase to remove selection cassettes from your genome engineering projects—use with HR Targeting Vectors that contain LoxP sites
Size:10 µg (Cat. # CRE100A-1)

pCOBLW (CAG-Oct4-Sox2-Klf4-Myc-GFP-SV40-Neo) Mouse Reprogramming PhiC31 Donor Plasmid

Popular for gene therapy applications, this PhiC31 Donor Plasmid comes with all the elements needed for non-viral generation of murine iPSCs
Size:10 µg (Cat. # FC305A-1)

pFC-MCS-pA-SV40-Neo PhiC31 Donor Vector

Use this PhiC31 Donor Vector, which contains only an MCS and a kanamycin/neomycin resistance gene, to clone-in your own promoter and gene-of-interest
Size:10 µg (Cat. # FC500A-1)

pFC-CMV-MCS-pA-SV40-Neo PhiC31 Donor Vector

Use this PhiC31 Donor Vector for non-viral transgenesis—drive your gene-of-interest with the CMV promoter and select for integrants with neomycin resistance
Size:10 µg (Cat. # FC501A-1)

pFC-CMV-GFP-SV40-Neo Positive Control Donor Vector

Use this PhiC31 Donor Vector which integrates GFP into the genome as a positive control for your PhiC31 Integrase System-based genome engineering projects
Size:10 µg (Cat. # FC520A-1)

pFC-EF1α-MCS-pA-PGK-RFP-T2A-Puro PhiC31 Donor Vector

Get the advantages of a bidirectional promoter with this PhiC31 Donor Vector that delivers an RFP marker and puromycin selection with your gene-of-interest
Size:10 µg (Cat. # FC550A-1)

pFC-PGK-MCS-pA-EF1α-GFP-T2A-Puro PhiC31 Donor Vector

Get the advantages of a bidirectional promoter with this PhiC31 Donor Vector that delivers a GFP marker and puromycin selection with your gene-of-interest
Size:10 µg (Cat. # FC551A-1)

pFC-CAG-MCS-WPRE-pA-SV40-Neo PhiC31 Donor Vector

Use this PhiC31 Donor Vector for non-viral transgenesis—drive your gene-of-interest with the CMV promoter and select for integrants with neomycin resistance
Size:10 µg (Cat. # FC600A-1)

PhiC31 Integrase Expression Plasmid

Put the integrase into your PhiC31 and PinPoint genome editing projects with this vector that expresses PhiC31 Integrase for one-step single-copy transgenesis
Size:10 µg (Cat. # FC200PA-1)

PhiC31 Integrase Mouse iPSC Reprogramming System

With both iPSC-generating donor and PhiC31 Integrase Expression plasmids, this kit delivers a complete, non-viral integrase-based iPSC generation system
Size:1 Kit (Cat. # FC300A-1)

PinPoint-HR attP Placement Vector Targeting the AAVS1 Safe Harbor Site

Place a PinPoint attP site into the AAVS1 Safe Harbor Site in the target genome with the CRISPR/Cas9 System and this PinPoint HR Targeting Vector
Size:10 µg (Cat. # PIN410A-1)

PinPoint-HR System Targeting AAVS1 for Platform Cell Line Generation & Retargeting

Get all the plasmids you need for PinPoint Targeted Integration using the CRISPR/Cas9 System to place the PinPoint attP site into the AAVS1 Safe Harbor Site
Size:1 Kit (Cat. # PIN412A-KIT)

EF1α PinPoint Donor Vector (pPP-EF1α-MCS-pA-attB-Puro-pA)

Deliver your gene-of-interest with this PinPoint Donor Vector – get single-copy integration at your pre-placed attP site and express your cDNA with EF1α
Size:10 µg (Cat. # PIN500A-1)

CAG PinPoint Donor Vector (pPP-CAG-MCS-WPRE-pA-attB-Puro-pA)

Deliver your gene-of-interest with this PinPoint Donor Vector – get single-copy integration at your pre-placed attP site and express your cDNA with CAG
Size:10 µg (Cat. # PIN510A-1)

Empty PinPoint Donor Vector (pPP-MCS-WPRE-pA-attB-Puro-pA)

Insert your expression cassette or other sequence with this PinPoint Donor Vector – get single-copy integration at your pre-placed attP site
Size:10 µg (Cat. # PIN520A-1)

CAG-GFP-T2A-Luc PinPoint Positive Control Donor Vector

Use this GFP and Luciferase-expressing PinPoint Donor Vector as a positive control or to troubleshoot your PinPoint Targeted Integration Projects
Size:10 µg (Cat. # PIN600A-1)

CMV-Luc PinPoint Positive Control Donor Vector

Use this Luciferase-expressing PinPoint Donor Vector as a positive control or to troubleshoot your PinPoint Targeted Integration Projects
Size:10 µg (Cat. # PIN610A-1)

PinPoint Integrase Expression Plasmid

Get controlled integration at a single, pre-placed site in the genome with the PinPoint Integrase for generation of isogenic cell lines and other applications
Size:10 µg (Cat. # PIN200A-1)

PinPoint-FC attP Placement Vector

Easily place the PinPoint attP site into a target genome with the PhiC31 Integrase Expression Plasmid and this PinPoint attP Placement Vector
Size:10 µg (Cat. # PIN300A-1)

PinPoint-FC System for Platform Cell Line Generation & Retargeting

Get all the plasmids you need for PinPoint Targeted Integration using the PhiC31 Integrase to place the PinPoint attP site into the target genome
Size:1 Kit (Cat. # PIN300A-KIT)