HR Targeting Vectors

Whether you’re using CRISPR/Cas9 for gene knock-ins, knock-outs, or edits, SBI’s ready-to-clone HR Targeting Vectors are carefully designed for easier workflows

Taking advantage of the cell’s double-strand break (DSB) repair system, homologous recombination (HR) targeting vectors (also known as HR donor vectors) can increase the efficiency of gene knock-outs, and are essential for gene knock-ins, edits, and fusions. SBI’s HR Targeting Vectors are all that and more, with features that can make it easier to find successful clones, minimize the amount of vector DNA left in the genome, minimize off-target integration, and more.

Need help choosing? Take a look at our table below, or contact technical support—we’re here to help you find the right product for your application, and can provide expert experimental support.

Catalog # HR Donor Vector Features* Application
Gene Knock-out Gene Knock-in Gene Edits Gene Tagging
HR100PA-1 MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4 Basic HR Donor
HR110PA-1 MCS1-EF1α-RFP-T2A-Puro-pA-MCS2 Removable RFP marker and puromycin selection
HR120PA-1 GFP-pA-LoxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCSPuro-pA-LoxP-MCS Tag with GFP fusion
Removable RFP marker and puromycin selection
HR130PA-1 T2A-GFP-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCSA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS Co-express GFP with “tagged” gene via T2A
Removable RFP marker and puromycin selection
HR150PA-1 GFP-T2A-Luc-pA-loxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS Tag with GFP fusion and co-express luciferase via T2A
Removable RFP marker and puromycin selection
HR180PA-1 IRES-GFP-pA-loxP-MCS1-EF1α-RFP-T2A-Puro-pA-LoxP-MCS2 Co-express GFP with “tagged” gene via IRES
Removable RFP marker and puromycin selection
HR210PA-1 MCS1-LoxP-EF1α-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2 Removable GFP marker, puromycin selection, and TK selection
HR220PA-1 GFP-pA-LoxP-EF1α-RFP-T2A-Hygro-pA-LoxP-MCS Tag with GFP fusion
Removable RFP ,arker and hygromycin Selection
HR410PA-1 MCS1-EF1α-GFP-T2A-Puro-pA-MCS2 Removable GFP marker and puromycin selection
HR510PA-1 MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2 Removable RFP marker and hygromycin selection
HR700PA-1 MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK Enrich for on-target integration with negative TK selection**
Removable GFP marker and puromycin selection
HR710PA-1 MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2-PGK-hsvTK Enrich for on-target integration with negative TK selection**
Removable RFP marker and hygromycin selection
HR720PA-1 MCS1-EF1α-Blasticidin-pA-MCS2-PGK-hsvTK Enrich for on-target integration with negative TK selection**
Removable blasticidin selection
GE602A-1 pAAVS1D-PGK-MCS-EF1α-copGFPpuro First generation AAVS1-targeting HR Donor
GE603A-1 pAAVS1D-CMV-RFP-EF1α-copGFPpuro First generation AAVS1-targeting HR Donor (positive control for GE602A-1)
GE620A-1 AAVS1-SA-puro-MCS Second generation AAVS1-targeting HR Donor
Promoterless to knock-in any gene or promoter-gene combination
GE622A-1 AAVS1-SA-puro-EF1α-MCS Second generation AAVS1-targeting HR Donor
Constitutive expression of your gene-of-interest
GE624A-1 AAVS1-SA-puro-MCS-GFP Second generation AAVS1-targeting HR Donor
Create reporter cell lines
CAS620A-1 AAVS1-SA-puro-EF1α-hspCas9 Knock-in Cas9 to the AAVS1 site
PBHR100A-1 MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2 Use with the PiggyBac Transposon System
Enables seamless gene editing with no residual footprint (i.e. completely remove vector sequences)
*All HR Target Vectors except PBHR100A-1 contain LoxP sites. Any sequences that are integrated between the two LoxP sites can be removed through transient expression of Cre Recombinase.
**The clever design of these HR Donors enables enrichment for on-target integration events. A PGK-hsvTK cassette is included outside of the homology arms. Because of this configuration, on-target integration that results from homologous recombination will not include the PGK-hsvTK cassette—only randomly-integrated off-target events will lead to integration of PGK-hsvTK and resulting TK activity. Therefore, TK selection will negatively select against off-target integrants. Click on any one of these vectors to see a diagram of how the negative selection works.

HR Targeting Vectors

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PrecisionXTM Basic HR Targeting Vector for Gene Knock-In/Out (MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4)

Knock-in any expression cassette to a specific genomic location with this basic HR targeting vector
Size:10 µg (Cat. # HR100PA-1)

DIY Cas9-expressing Cells HR Donor

Streamline gRNA screening, functional validation and more—make your own Cas9-expressing cell lines with this HR donor that knocks-in Cas9 to the AAVS1 site.
Size:10 µg (Cat. # CAS620A-1)

DIY Cas9-expressing Cells Complete Kit

Streamline gRNA screening, functional validation and more—get everything you need to make your own cell lines expressing Cas9 from the AAVS1 site.
Size:1 Kit (Cat. # CAS620A-KIT)

PrecisionXTM Gene Knock-out HR Targeting Vector (MCS1-EF1α-RFP-T2A-Puro-pA-MCS2)

Knock-out any gene or edit the genome with this HR targeting vector—features an RFP marker and puromycin selection as a removable selection cassette.
Size:10 µg (Cat. # HR110PA-1)

PrecisionXTM Gene Knock-out HR Targeting Vector (MCS1-LoxP-EF1α-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2)

Knock-out or edit any gene with this HR targeting vector—features a GFP marker, puromycin selection, and TK selection as a removable selection cassette.
Size:10 µg (Cat. # HR210PA-1)

PrecisionXTM Gene Knock-out HR Targeting Vector (MCS1-EF1α-GFP-T2A-Puro-pA-MCS2)

Knock-out or edit any gene with this HR targeting vector—features a GFP marker and puromycin selection as a removable selection cassette.
Size:10 µg (Cat. # HR410PA-1)

PrecisionXTM Gene Knock-out HR Targeting Vector (MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2)

Knock-out or edit any gene with this HR targeting vector—features an RFP marker and hygromycin selection as a removable selection cassette.
Size:10 µg (Cat. # HR510PA-1)

PrecisionXTM OnTargetTM Gene Knock-out HR Targeting Vector (MCS1-EF1α-GFP-T2A-Puro-pA-MCS2-PGK-hsvTK)

Enrich for on-target integrants via negative TK selection with this knock-out and editing HR targeting vector—also features GFP and puromycin
Size:10 µg (Cat. # HR700PA-1)

PrecisionXTM OnTargetTM Gene Knock-out HR Targeting Vector (MCS1-EF1α-RFP-T2A-Hygro-pA-MCS2-PGK-hsvTK)

Enrich for on-target integrants via negative TK selection with this knock-out and editing HR targeting vector—also features RFP and hygromycin
Size:10 µg (Cat. # HR710PA-1)

PrecisionXTM OnTargetTM Gene Knock-out HR Targeting Vector (MCS1-EF1α-Blasticidin-pA-MCS2-PGK-hsvTK)

Enrich for on-target integrants via negative TK selection with this knock-out and editing HR targeting vector—also features blasticidin selection
Size:10 µg (Cat. # HR720PA-1)

PiggyBac Gene Editing HR Targeting Vector (MCS1-5'PB TR-EF1α-GFP-T2A-Puro-T2A-hsvTK-pA-3' PB TR-MCS2)

Without a trace—make seamless gene edits with no residual footprint—includes dual GFP/puromycin selection and on-target enrichment with TK selection
Size:10 µg (Cat. # PBHR100A-1)

PrecisionXTM Gene Tagging HR Targeting Vector (GFP-pA-LoxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS)

Tag a gene or create a reporter GFP-fusion with this HR targeting vector—features an RFP marker and puromycin selection as a removable selection cassette.
Size:10 µg (Cat. # HR120PA-1)

PrecisionXTM Gene Tagging HR Targeting Vector (T2A-GFP-pA-LoxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS)

Co-express a GFP reporter with any gene with this HR targeting vector—features an RFP marker and puromycin selection as a removable selection cassette.
Size:10 µg (Cat. # HR130PA-1)

PrecisionXTM Gene Tagging HR Targeting Vector (GFP-T2A-Luc-pA-LoxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS)

Fuse a GFP reporter and co-express luciferase with any gene using this HR targeting vector—features RFP and puromycin as a removable selection cassette.
Size:10 µg (Cat. # HR150PA-1)

PrecisionXTM Gene Tagging HR Targeting Vector (IRES-GFP-pA-LoxP-EF1α-RFP-T2A-Puro-pA-LoxP-MCS)

Co-express GFP (using IRES) with this HR targeting vector—features an RFP marker and puromycin selection as a removable selection cassette.
Size:10 µg (Cat. # HR180PA-1)

PrecisionXTM Gene Tagging HR Targeting Vector (GFP-pA-LoxP-EF1α-RFP-T2A-Hygro-pA-LoxP-MCS)

Create a GFP fusion to any gene with this HR targeting vector—features an RFP marker and hygromycin selection as a removable selection cassette.
Size:10 µg (Cat. # HR220PA-1)

pCMV-CRE, Cre Recombinase Expression Plasmid

Transiently express Cre recombinase to remove selection cassettes from your genome engineering projects—use with HR Targeting Vectors that contain LoxP sites
Size:10 µg (Cat. # CRE100A-1)