When you need to know the amount of exosomes you’ve isolated, SBI offers a range of options that meet most quantitation needs. For the fastest and most sensitive antibody-based quantitation, our new ExoELISA™-ULTRA CD63 kit improves on our original ExoELISA Kits by providing greater sensitivity—down to 1-200 µg protein equivalent—and a fast, 4-hour workflow.
If you want speedier quantitation (20 min) that does not rely on antibody binding, the EXOCET™ kit provides direct measurement of the activity of a known exosomal protein, Acetylcholinesterase (AChE). FluoroCet is a >30-fold more sensitive version of EXOCET that relies upon fluorescence-based quantitation.
|* No overnight incubation necessary|
|Use||For fast and sensitive antibody-based quantitation of exosomes||For sensitive quantitation of exosomes when time is not limiting||For fast quantitation of extracellular vesicles with moderate sample input requirements||For the most sensitive detection with very low sample input requirements|
|Detection Method||Antibody||Antibody||Enzymatic, colorimetric||Enzymatic, fluorescent|
|Protocol Time||4 hours*||24 hours||20 minutes||60 minutes|
|Input amount||1–200 µg protein equivalent||50-100 µg protein equivalent||50 µg protein equivalent||<1 µg protein equivalent|