ExoELISA-ULTRA Complete Kit (CD63 Detection)

With a 4-hour total assay time, this sensitive ELISA-based assay speeds quantitation of exosomes from most biofluids
  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

Products

Catalog Number Description Size Price Quantity Add to Cart
EXEL-ULTRA-CD63-1 ExoELISA-ULTRA Complete Kit (CD63 detection) 96 Reactions $636
- +

Overview

Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Choose the exosome quantitation method that’s best for your studies
 ExoELISA-ULTRA Complete KitsExoELISA KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor sensitive quantitation of exosomes when time and input sample are not limitingFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)Enzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)24 hours20 min60 min
Input sample amount (protein equivalent)1 – 200 µg>500 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCETFluoroCet
REFERENCES
  1. Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.

How It Works

Supporting Data

Supporting Data

The standard curve for ExoELISA-ULTRA CD63 shows robust linearity down to ~1 x 109 exosomes. The standard curve for ExoELISA-ULTRA CD63 provides robust linearity down to ~1 x 10^9 exosomes.

Resources

Citations

  • Gandham, SK, Attarwala, HZ & Amiji, MM. (2022) Mathematical Modeling and Experimental Validation of Extracellular Vesicle-Mediated Tumor Suppressor MicroRNA Delivery and Propagation in Ovarian Cancer Cells. Molecular pharmaceutics. 1970 Jan 1;. PM ID: 36226722
  • Yoon, S, Bogdanov, K & Wallach, D. (2022) Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell death and differentiation. 1970 Jan 1;. PM ID: 34999730
  • Cilibrasi, C, et al. (2022) Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines. 1970 Jan 1; 10(1). PM ID: 35052804
  • Melling, GE, et al. (2022) Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods. Scientific reports. 1970 Jan 1; 12(1):262. PM ID: 34997141
  • Chetty, V, et al. (2022) Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer. Cancers. 1970 Jan 1; 14(9):2068. Link: Cancers
  • Hong, L, et al. (2022) Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer. Journal of clinical laboratory analysis. 1970 Jan 1;:e24447. PM ID: 35446993
  • Li, Y, et al. (2022) Mouse mesenchymal stem cell-derived exosomal miR-466f-3p reverses EMT process through inhibiting AKT/GSK3β pathway via c-MET in radiation-induced lung injury. Journal of experimental & clinical cancer research : CR. 1970 Jan 1; 41(1):128. PM ID: 35392967
  • Xiao, W, et al. (2022) Matrix stiffness mediates pancreatic cancer chemoresistance through induction of exosome hypersecretion in a cancer associated fibroblasts-tumor organoid biomimetic model. Matrix biology plus. 1970 Jan 1; 14:100111. PM ID: 35619988
  • Rodrigues, LJS. (2022) Characterization of extracellular vesicles from ascitic fluid in ovarian cancer patients. Thesis. 1970 Jan 1;. Link: Thesis
  • Hrdinova, T. (2022) Molecular events associated with resistance to tyrosine kinase inhibitors in leukemia cells.. Thesis. 1970 Jan 1;. Link: Thesis
  • Blommer, J, et al. (2022) Extracellular vesicle biomarkers for cognitive impairment in Parkinson’s disease. Brain : a journal of neurology. 1970 Jan 1;. PM ID: 35833836
  • Yu, HY, et al. (2022) Exosomes from PM 2.5-treated Human Bronchial Epithelial Cells Increase Lung Cancer Metastatic Potential. Biomedical and environmental sciences : BES. 1970 Jan 1; 35(6):473-484. PM ID: 35882407
  • Meliciano, AEL. (2022) Empowering the therapeutic potential of clinically expired platelet concentrates: a new source of extracellular vesicles. Thesis. 1970 Jan 1;. Link: Thesis
  • Jayathilaka, EHTT, et al. (2022) Isolation and characterization of plasma-derived exosomes from olive flounder (Paralichthys olivaceus) and their wound healing and regeneration activities. Fish & shellfish immunology. 1970 Jan 1; 128:196-205. PM ID: 35932983
  • Kim, CG, et al. (2022) Small RNA sequencing of small extracellular vesicles secreted by umbilical cord mesenchymal stem cells following replicative senescence. Genes & genomics. 1970 Jan 1;. PM ID: 35917089
  • Freitas, R. (2022) Avaliação do perfil de microRNAs exossomais em portadores de hipercolesterolemia familial. Universidade de São Paulo. 1970 Jan 1;. Link: Universidade de São Paulo
  • André-Grégoire, G, et al. (2022) Inhibition of the pseudokinase MLKL alters extracellular vesicle release and reduces tumor growth in glioblastoma. iScience. 1970 Jan 1; 25(10):105118. Link: iScience
  • Zhang, W, Zhang, J & Huang, H. (2022) Exosomes from adipose-derived stem cells inhibit inflammation and oxidative stress in LPS-acute kidney injury. Experimental cell research. 1970 Jan 1; 420(1):113332. PM ID: 36084668
  • Andreu, Z, et al. (2022) A Rapid, Convergent Approach to the Identification of Exosome Inhibitors in Breast Cancer Models. Nanotheranostics. 1970 Jan 1; 7(1):1-21. Link: Nanotheranostics
  • Kuhn, M, et al. (2022) IMP1/IGF2BP1 in human colorectal cancer extracellular vesicles. American journal of physiology. Gastrointestinal and liver physiology. 1970 Jan 1;. PM ID: 36194131

Products

Catalog Number Description Size Price Quantity Add to Cart
EXEL-ULTRA-CD63-1 ExoELISA-ULTRA Complete Kit (CD63 detection) 96 Reactions $636
- +

Overview

Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Choose the exosome quantitation method that’s best for your studies
 ExoELISA-ULTRA Complete KitsExoELISA KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor sensitive quantitation of exosomes when time and input sample are not limitingFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)Enzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)24 hours20 min60 min
Input sample amount (protein equivalent)1 – 200 µg>500 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCETFluoroCet
REFERENCES
  1. Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.

How It Works

Supporting Data

Supporting Data

The standard curve for ExoELISA-ULTRA CD63 shows robust linearity down to ~1 x 109 exosomes. The standard curve for ExoELISA-ULTRA CD63 provides robust linearity down to ~1 x 10^9 exosomes.

Citations

  • Gandham, SK, Attarwala, HZ & Amiji, MM. (2022) Mathematical Modeling and Experimental Validation of Extracellular Vesicle-Mediated Tumor Suppressor MicroRNA Delivery and Propagation in Ovarian Cancer Cells. Molecular pharmaceutics. 1970 Jan 1;. PM ID: 36226722
  • Yoon, S, Bogdanov, K & Wallach, D. (2022) Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell death and differentiation. 1970 Jan 1;. PM ID: 34999730
  • Cilibrasi, C, et al. (2022) Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines. 1970 Jan 1; 10(1). PM ID: 35052804
  • Melling, GE, et al. (2022) Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods. Scientific reports. 1970 Jan 1; 12(1):262. PM ID: 34997141
  • Chetty, V, et al. (2022) Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer. Cancers. 1970 Jan 1; 14(9):2068. Link: Cancers
  • Hong, L, et al. (2022) Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer. Journal of clinical laboratory analysis. 1970 Jan 1;:e24447. PM ID: 35446993
  • Li, Y, et al. (2022) Mouse mesenchymal stem cell-derived exosomal miR-466f-3p reverses EMT process through inhibiting AKT/GSK3β pathway via c-MET in radiation-induced lung injury. Journal of experimental & clinical cancer research : CR. 1970 Jan 1; 41(1):128. PM ID: 35392967
  • Xiao, W, et al. (2022) Matrix stiffness mediates pancreatic cancer chemoresistance through induction of exosome hypersecretion in a cancer associated fibroblasts-tumor organoid biomimetic model. Matrix biology plus. 1970 Jan 1; 14:100111. PM ID: 35619988
  • Rodrigues, LJS. (2022) Characterization of extracellular vesicles from ascitic fluid in ovarian cancer patients. Thesis. 1970 Jan 1;. Link: Thesis
  • Hrdinova, T. (2022) Molecular events associated with resistance to tyrosine kinase inhibitors in leukemia cells.. Thesis. 1970 Jan 1;. Link: Thesis
  • Blommer, J, et al. (2022) Extracellular vesicle biomarkers for cognitive impairment in Parkinson’s disease. Brain : a journal of neurology. 1970 Jan 1;. PM ID: 35833836
  • Yu, HY, et al. (2022) Exosomes from PM 2.5-treated Human Bronchial Epithelial Cells Increase Lung Cancer Metastatic Potential. Biomedical and environmental sciences : BES. 1970 Jan 1; 35(6):473-484. PM ID: 35882407
  • Meliciano, AEL. (2022) Empowering the therapeutic potential of clinically expired platelet concentrates: a new source of extracellular vesicles. Thesis. 1970 Jan 1;. Link: Thesis
  • Jayathilaka, EHTT, et al. (2022) Isolation and characterization of plasma-derived exosomes from olive flounder (Paralichthys olivaceus) and their wound healing and regeneration activities. Fish & shellfish immunology. 1970 Jan 1; 128:196-205. PM ID: 35932983
  • Kim, CG, et al. (2022) Small RNA sequencing of small extracellular vesicles secreted by umbilical cord mesenchymal stem cells following replicative senescence. Genes & genomics. 1970 Jan 1;. PM ID: 35917089
  • Freitas, R. (2022) Avaliação do perfil de microRNAs exossomais em portadores de hipercolesterolemia familial. Universidade de São Paulo. 1970 Jan 1;. Link: Universidade de São Paulo
  • André-Grégoire, G, et al. (2022) Inhibition of the pseudokinase MLKL alters extracellular vesicle release and reduces tumor growth in glioblastoma. iScience. 1970 Jan 1; 25(10):105118. Link: iScience
  • Zhang, W, Zhang, J & Huang, H. (2022) Exosomes from adipose-derived stem cells inhibit inflammation and oxidative stress in LPS-acute kidney injury. Experimental cell research. 1970 Jan 1; 420(1):113332. PM ID: 36084668
  • Andreu, Z, et al. (2022) A Rapid, Convergent Approach to the Identification of Exosome Inhibitors in Breast Cancer Models. Nanotheranostics. 1970 Jan 1; 7(1):1-21. Link: Nanotheranostics
  • Kuhn, M, et al. (2022) IMP1/IGF2BP1 in human colorectal cancer extracellular vesicles. American journal of physiology. Gastrointestinal and liver physiology. 1970 Jan 1;. PM ID: 36194131