ExoELISA Complete Kit (CD63 Detection)
- Sensitive—detect as little as 500 µg protein equivalent
- Flexible—compatible with all major exosome isolation methods (g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
Products
Overview
Overview
Sensitive, ELISA-based exosome quantitation
When you would like to use an ELISA-based method to find out how many exosomes you have and time is not limiting, choose one of SBI’s ExoELISA Complete Kits. Calibrated using NanoSight Analysis, the ExoELISA Complete Kit (CD63 Detection) enables calculation of exosome abundance based on the presence of CD63, a general exosome marker. Note that our newer ExoELISA-ULTRA Complete Kit (CD63 Detection) can provide faster exosome quantitation using significantly less sample.
- Sensitive—detect as little as 500 µg protein equivalent
- Flexible—compatible with all major exosome isolation methods (g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
How It Works
How It Works
Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.
The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody, anti-CD63. Following addition of anti-CD63, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.
The amount of CD63 is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of CD63 present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.
Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying CD63 from the ExoELISA data.
Supporting Data
FAQs
Resources
Related Products
Citations
-
Pallares-Rusiñol, A, et al. (2023) Advances in exosome analysis. Advances in clinical chemistry. 2023; 112:69-117. PM ID: 36642486
-
Lee, S, et al. (2023) Mesenchymal stem cell-derived extracellular vesicles subvert Th17 cells by destabilizing RORγt through posttranslational modification. Experimental & molecular medicine. 2023;. PM ID: 36964252
-
Cai, J, et al. (2023) Exosomes Derived From Kartogenin-Preconditioned Mesenchymal Stem Cells Promote Cartilage Formation and Collagen Maturation for Enthesis Regeneration in a Rat Model of Chronic Rotator Cuff Tear. The American journal of sports medicine. 2023; 51(5):1267-1276. PM ID: 36917828
-
Otahal, A, et al. (2023) Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy. Methods in molecular biology (Clifton, N.J.). 2023; 2598:123-140. PM ID: 36355289
-
Guo, Q, et al. (2023) Glioblastoma upregulates SUMOylation of hnRNP A2/B1 to eliminate the tumor suppressor miR-204-3p, accelerating angiogenesis under hypoxia. Cell death & disease. 2023; 14(2):147. PM ID: 36810326
-
von Stade, DP, et al. (2023) Exosome cell origin affects in vitro markers of tendon repair in ovine macrophages and tenocytes. Tissue engineering. Part A. 2023;. PM ID: 36792933
-
Pradhan, A, et al. (2023) Association of exosomal miR-96-5p and miR-146a-5p with the disease severity in dengue virus infection. Journal of medical virology. 2023; 95(3):e28614. PM ID: 36840403
-
Meliciano, A, et al. (2023) Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery. Pharmaceutics. 2023; 15(3). PM ID: 36986815
-
Cui, H, et al. (2023) Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin. International Journal of Molecular Sciences. 2023; 24(7):6132. Link: International Journal of Molecular Sciences
-
Shen, S, et al. (2023) Effects of lysate/tissue storage at -80°C on subsequently extracted EVs of epithelial ovarian cancer tissue origins. iScience. 2023;:106521. Link: iScience
-
Gandham, SK, Attarwala, HZ & Amiji, MM. (2022) Mathematical Modeling and Experimental Validation of Extracellular Vesicle-Mediated Tumor Suppressor MicroRNA Delivery and Propagation in Ovarian Cancer Cells. Molecular pharmaceutics. 2022;. PM ID: 36226722
-
Bazoer, J. (2022) Regulatory T Cell Derived EVs-Designing Novel Immune Based Therapies to Prolong Lifespan of Transplanted Tissue. Thesis. 2022;. Link: Thesis
-
Ntsethe, A & Mackraj, I. (2022) An Investigation of Exosome Concentration and Exosomal microRNA (miR-155 and miR-222) Expression in Pregnant Women with Gestational Hypertension and Preeclampsia. International journal of women's health. 2022; 14:1681-1689. PM ID: 36514348
-
Yoon, S, Bogdanov, K & Wallach, D. (2022) Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell death and differentiation. 2022;. PM ID: 34999730
-
Cilibrasi, C, et al. (2022) Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines. 2022; 10(1). PM ID: 35052804
-
Melling, GE, et al. (2022) Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods. Scientific reports. 2022; 12(1):262. PM ID: 34997141
-
Chetty, V, et al. (2022) Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer. Cancers. 2022; 14(9):2068. Link: Cancers
-
Hong, L, et al. (2022) Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer. Journal of clinical laboratory analysis. 2022;:e24447. PM ID: 35446993
-
Li, Y, et al. (2022) Mouse mesenchymal stem cell-derived exosomal miR-466f-3p reverses EMT process through inhibiting AKT/GSK3β pathway via c-MET in radiation-induced lung injury. Journal of experimental & clinical cancer research : CR. 2022; 41(1):128. PM ID: 35392967
-
Xiao, W, et al. (2022) Matrix stiffness mediates pancreatic cancer chemoresistance through induction of exosome hypersecretion in a cancer associated fibroblasts-tumor organoid biomimetic model. Matrix biology plus. 2022; 14:100111. PM ID: 35619988
- See More
Products
Overview
Overview
Sensitive, ELISA-based exosome quantitation
When you would like to use an ELISA-based method to find out how many exosomes you have and time is not limiting, choose one of SBI’s ExoELISA Complete Kits. Calibrated using NanoSight Analysis, the ExoELISA Complete Kit (CD63 Detection) enables calculation of exosome abundance based on the presence of CD63, a general exosome marker. Note that our newer ExoELISA-ULTRA Complete Kit (CD63 Detection) can provide faster exosome quantitation using significantly less sample.
- Sensitive—detect as little as 500 µg protein equivalent
- Flexible—compatible with all major exosome isolation methods (g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
How It Works
How It Works
Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compatible with exosomes isolated using most methods, including ExoQuick®, ExoQuick-TC®, or ultracentrifugation.
The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody, anti-CD63. Following addition of anti-CD63, a secondary antibody (goat anti-rabbit) linked to horseradish peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.
The amount of CD63 is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of CD63 present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.
Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying CD63 from the ExoELISA data.
Supporting Data
FAQs
Citations
-
Pallares-Rusiñol, A, et al. (2023) Advances in exosome analysis. Advances in clinical chemistry. 2023; 112:69-117. PM ID: 36642486
-
Lee, S, et al. (2023) Mesenchymal stem cell-derived extracellular vesicles subvert Th17 cells by destabilizing RORγt through posttranslational modification. Experimental & molecular medicine. 2023;. PM ID: 36964252
-
Cai, J, et al. (2023) Exosomes Derived From Kartogenin-Preconditioned Mesenchymal Stem Cells Promote Cartilage Formation and Collagen Maturation for Enthesis Regeneration in a Rat Model of Chronic Rotator Cuff Tear. The American journal of sports medicine. 2023; 51(5):1267-1276. PM ID: 36917828
-
Otahal, A, et al. (2023) Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy. Methods in molecular biology (Clifton, N.J.). 2023; 2598:123-140. PM ID: 36355289
-
Guo, Q, et al. (2023) Glioblastoma upregulates SUMOylation of hnRNP A2/B1 to eliminate the tumor suppressor miR-204-3p, accelerating angiogenesis under hypoxia. Cell death & disease. 2023; 14(2):147. PM ID: 36810326
-
von Stade, DP, et al. (2023) Exosome cell origin affects in vitro markers of tendon repair in ovine macrophages and tenocytes. Tissue engineering. Part A. 2023;. PM ID: 36792933
-
Pradhan, A, et al. (2023) Association of exosomal miR-96-5p and miR-146a-5p with the disease severity in dengue virus infection. Journal of medical virology. 2023; 95(3):e28614. PM ID: 36840403
-
Meliciano, A, et al. (2023) Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery. Pharmaceutics. 2023; 15(3). PM ID: 36986815
-
Cui, H, et al. (2023) Effect of Hypertrophic Scar Fibroblast-Derived Exosomes on Keratinocytes of Normal Human Skin. International Journal of Molecular Sciences. 2023; 24(7):6132. Link: International Journal of Molecular Sciences
-
Shen, S, et al. (2023) Effects of lysate/tissue storage at -80°C on subsequently extracted EVs of epithelial ovarian cancer tissue origins. iScience. 2023;:106521. Link: iScience
-
Gandham, SK, Attarwala, HZ & Amiji, MM. (2022) Mathematical Modeling and Experimental Validation of Extracellular Vesicle-Mediated Tumor Suppressor MicroRNA Delivery and Propagation in Ovarian Cancer Cells. Molecular pharmaceutics. 2022;. PM ID: 36226722
-
Bazoer, J. (2022) Regulatory T Cell Derived EVs-Designing Novel Immune Based Therapies to Prolong Lifespan of Transplanted Tissue. Thesis. 2022;. Link: Thesis
-
Ntsethe, A & Mackraj, I. (2022) An Investigation of Exosome Concentration and Exosomal microRNA (miR-155 and miR-222) Expression in Pregnant Women with Gestational Hypertension and Preeclampsia. International journal of women's health. 2022; 14:1681-1689. PM ID: 36514348
-
Yoon, S, Bogdanov, K & Wallach, D. (2022) Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes. Cell death and differentiation. 2022;. PM ID: 34999730
-
Cilibrasi, C, et al. (2022) Definition of an Inflammatory Biomarker Signature in Plasma-Derived Extracellular Vesicles of Glioblastoma Patients. Biomedicines. 2022; 10(1). PM ID: 35052804
-
Melling, GE, et al. (2022) Confocal microscopy analysis reveals that only a small proportion of extracellular vesicles are successfully labelled with commonly utilised staining methods. Scientific reports. 2022; 12(1):262. PM ID: 34997141
-
Chetty, V, et al. (2022) Efficient Small Extracellular Vesicles (EV) Isolation Method and Evaluation of EV-Associated DNA Role in Cell-Cell Communication in Cancer. Cancers. 2022; 14(9):2068. Link: Cancers
-
Hong, L, et al. (2022) Exosomal circular RNA hsa_circ_0006220, and hsa_circ_0001666 as biomarkers in the diagnosis of pancreatic cancer. Journal of clinical laboratory analysis. 2022;:e24447. PM ID: 35446993
-
Li, Y, et al. (2022) Mouse mesenchymal stem cell-derived exosomal miR-466f-3p reverses EMT process through inhibiting AKT/GSK3β pathway via c-MET in radiation-induced lung injury. Journal of experimental & clinical cancer research : CR. 2022; 41(1):128. PM ID: 35392967
-
Xiao, W, et al. (2022) Matrix stiffness mediates pancreatic cancer chemoresistance through induction of exosome hypersecretion in a cancer associated fibroblasts-tumor organoid biomimetic model. Matrix biology plus. 2022; 14:100111. PM ID: 35619988
- See More