ExoELISA-ULTRA Complete Kit (CD63 Detection)
With a 4-hour total assay time, this sensitive ELISA-based assay speeds quantitation of exosomes from most biofluids
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-CD63-1 | ExoELISA-ULTRA Complete Kit (CD63 detection) | 96 Reactions | $673 |
|
||||
Overview
Overview
Delivering ELISA-based exosome quantitation ULTRA fast
Improving on our popular ExoELISA™ Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.
Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
- Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.
How It Works
Supporting Data
Supporting Data
The standard curve for ExoELISA-ULTRA CD63 shows robust linearity down to ~1 x 109 exosomes.
FAQs
Resources
Related Products
Citations
-
Han, D, et al. (2024) Current Technology for Production, Isolation, and Quality Control of Extracellular Vesicles. Biomedical Applications of Extracellular Vesicles. 2024;:117-146. Link: Biomedical Applications of Extracellular Vesicles
-
Bhagwan Valjee, R, et al. (2024) Investigation of exosomal tetraspanin profile in sepsis patients as a promising diagnostic biomarker. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 2024;:1-12. PM ID: 38354024
-
Gao, H, et al. (2024) Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism. Journal of extracellular vesicles. 2024; 13(1):e12401. PM ID: 38151470
-
Byappanahalli, A, et al. (2024) Extracellular vesicle mitochondrial DNA levels are associated with race and mitochondrial DNA haplogroup. iScience. 2024; 27(1):108724. Link: iScience
-
Nagao, Y, et al. (2024) Uterine leiomyosarcoma cell-derived extracellular vesicles induce the formation of cancer-associated fibroblasts. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2024;:167103. PM ID: 38417460
-
Rajeev Kumar, S, Sakthiswary, R & Lokanathan, Y. (2024) Potential Therapeutic Application and Mechanism of Action of Stem Cell-Derived Extracellular Vesicles (EVs) in Systemic Lupus Erythematosus (SLE). International journal of molecular sciences. 2024; 25(4). PM ID: 38397121
-
Huang, Y, et al. (2024) Identification of a Serum Exosome-Derived lncRNA‒miRNA‒mRNA ceRNA Network in Patients with Endometriosis. Clinical and Experimental Obstetrics & Gynecology. 2024; 51(2):51. Link: Clinical and Experimental Obstetrics & Gynecology
-
Muraoka, A, et al. (2024) Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology. Communications medicine. 2024; 4(1):33. PM ID: 38418565
-
Liang, W, Najor, RH & Gustafsson, ÅB. (2024) Protocol to separate small and large extracellular vesicles from mouse and human cardiac tissues. STAR protocols. 2024; 5(1):102914. PM ID: 38386549
-
Wang, L, et al. (2024) Dual amplified electrochemical sensing coupling of ternary hybridization-based exosomal microRNA recognition and perchlorate-assisted electrocatalytic cycle. Biosensors and Bioelectronics. 2024; 243:115783. Link: Biosensors and Bioelectronics
-
Dias, T, et al. (2024) An Electro-Optical Technology for the Ultrasensitive Detection of Small Extracellular Vesicle Sub-Populations and Their Protein Epitope Counts. Available at SSRN . 2024;. Link: Available at SSRN
-
Pallares-Rusiñol, A, et al. (2023) Advances in exosome analysis. Advances in clinical chemistry. 2023; 112:69-117. PM ID: 36642486
-
Lee, S, et al. (2023) Mesenchymal stem cell-derived extracellular vesicles subvert Th17 cells by destabilizing RORγt through posttranslational modification. Experimental & molecular medicine. 2023;. PM ID: 36964252
-
Cai, J, et al. (2023) Exosomes Derived From Kartogenin-Preconditioned Mesenchymal Stem Cells Promote Cartilage Formation and Collagen Maturation for Enthesis Regeneration in a Rat Model of Chronic Rotator Cuff Tear. The American journal of sports medicine. 2023; 51(5):1267-1276. PM ID: 36917828
-
Nguyen, CM, et al. (2023) Placental Exosomes as Biomarkers for Maternal Diseases: Current Advances in Isolation, Characterization, and Detection. ACS sensors. 2023; 8(7):2493-2513. PM ID: 37449399
-
Taha, H. (2023) Biomarkers in CNS-originating Extracellular Vesicles for Parkinson’s disease and Multiple System Atrophy. Thesis. 2023;. Link: Thesis
-
Ayala-Mar, S & Gonzalez-Valdez, J. (2023) Research and Development of Emerging Technologies for Exosome-based Cancer Diagnostics and Therapeutics. laccei.org. 2023;. Link: laccei.org
-
Rowart, P, et al. (2023) Fast and Efficient Isolation of Exosomes from Stem Cells Using a Combination of Single-Use Bioreactors, High-Speed-and Ultracentrifugation. eppendorf.com. 2023;. Link: eppendorf.com
-
Otahal, A, et al. (2023) Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy. Methods in molecular biology (Clifton, N.J.). 2023; 2598:123-140. PM ID: 36355289
-
Guo, Q, et al. (2023) Glioblastoma upregulates SUMOylation of hnRNP A2/B1 to eliminate the tumor suppressor miR-204-3p, accelerating angiogenesis under hypoxia. Cell death & disease. 2023; 14(2):147. PM ID: 36810326
- See More
ExoELISA-ULTRA Complete Kit (CD63 Detection)
$673.00
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXEL-ULTRA-CD63-1 | ExoELISA-ULTRA Complete Kit (CD63 detection) | 96 Reactions | $673 |
|
||||
Overview
Overview
Delivering ELISA-based exosome quantitation ULTRA fast
Improving on our popular ExoELISA™ Kits, the ExoELISA-ULTRA CD63 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.
Currently configured for detection of CD63, a widely recognized and popular exosomal marker1, ExoELISA-ULTRA CD63 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with nearly all biofluids. The ExoELISA-ULTRA CD63 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NanoSight analysis and enables quantitation of exosomes carrying CD63 in your target samples. One ExoELISA-ULTRA CD63 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.
- Sensitive—detect as little as 1 µg protein equivalent
- Fast—complete in less than 4-hours—no more overnight incubation
- Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick®, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
- Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD63
- Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
| ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
|---|---|---|---|
| Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
| Detection method | Antibody | Enzymatic | Enzymatic |
| Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
| Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
| Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
| Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
- Kowal, J., et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016. February 23. 113(8): E968–E977. PMCID: PMC4776515.
How It Works
Supporting Data
Supporting Data
The standard curve for ExoELISA-ULTRA CD63 shows robust linearity down to ~1 x 109 exosomes.FAQs
Citations
-
Han, D, et al. (2024) Current Technology for Production, Isolation, and Quality Control of Extracellular Vesicles. Biomedical Applications of Extracellular Vesicles. 2024;:117-146. Link: Biomedical Applications of Extracellular Vesicles
-
Bhagwan Valjee, R, et al. (2024) Investigation of exosomal tetraspanin profile in sepsis patients as a promising diagnostic biomarker. Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. 2024;:1-12. PM ID: 38354024
-
Gao, H, et al. (2024) Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism. Journal of extracellular vesicles. 2024; 13(1):e12401. PM ID: 38151470
-
Byappanahalli, A, et al. (2024) Extracellular vesicle mitochondrial DNA levels are associated with race and mitochondrial DNA haplogroup. iScience. 2024; 27(1):108724. Link: iScience
-
Nagao, Y, et al. (2024) Uterine leiomyosarcoma cell-derived extracellular vesicles induce the formation of cancer-associated fibroblasts. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 2024;:167103. PM ID: 38417460
-
Rajeev Kumar, S, Sakthiswary, R & Lokanathan, Y. (2024) Potential Therapeutic Application and Mechanism of Action of Stem Cell-Derived Extracellular Vesicles (EVs) in Systemic Lupus Erythematosus (SLE). International journal of molecular sciences. 2024; 25(4). PM ID: 38397121
-
Huang, Y, et al. (2024) Identification of a Serum Exosome-Derived lncRNA‒miRNA‒mRNA ceRNA Network in Patients with Endometriosis. Clinical and Experimental Obstetrics & Gynecology. 2024; 51(2):51. Link: Clinical and Experimental Obstetrics & Gynecology
-
Muraoka, A, et al. (2024) Small extracellular vesicles in follicular fluids for predicting reproductive outcomes in assisted reproductive technology. Communications medicine. 2024; 4(1):33. PM ID: 38418565
-
Liang, W, Najor, RH & Gustafsson, ÅB. (2024) Protocol to separate small and large extracellular vesicles from mouse and human cardiac tissues. STAR protocols. 2024; 5(1):102914. PM ID: 38386549
-
Wang, L, et al. (2024) Dual amplified electrochemical sensing coupling of ternary hybridization-based exosomal microRNA recognition and perchlorate-assisted electrocatalytic cycle. Biosensors and Bioelectronics. 2024; 243:115783. Link: Biosensors and Bioelectronics
-
Dias, T, et al. (2024) An Electro-Optical Technology for the Ultrasensitive Detection of Small Extracellular Vesicle Sub-Populations and Their Protein Epitope Counts. Available at SSRN . 2024;. Link: Available at SSRN
-
Pallares-Rusiñol, A, et al. (2023) Advances in exosome analysis. Advances in clinical chemistry. 2023; 112:69-117. PM ID: 36642486
-
Lee, S, et al. (2023) Mesenchymal stem cell-derived extracellular vesicles subvert Th17 cells by destabilizing RORγt through posttranslational modification. Experimental & molecular medicine. 2023;. PM ID: 36964252
-
Cai, J, et al. (2023) Exosomes Derived From Kartogenin-Preconditioned Mesenchymal Stem Cells Promote Cartilage Formation and Collagen Maturation for Enthesis Regeneration in a Rat Model of Chronic Rotator Cuff Tear. The American journal of sports medicine. 2023; 51(5):1267-1276. PM ID: 36917828
-
Nguyen, CM, et al. (2023) Placental Exosomes as Biomarkers for Maternal Diseases: Current Advances in Isolation, Characterization, and Detection. ACS sensors. 2023; 8(7):2493-2513. PM ID: 37449399
-
Taha, H. (2023) Biomarkers in CNS-originating Extracellular Vesicles for Parkinson’s disease and Multiple System Atrophy. Thesis. 2023;. Link: Thesis
-
Ayala-Mar, S & Gonzalez-Valdez, J. (2023) Research and Development of Emerging Technologies for Exosome-based Cancer Diagnostics and Therapeutics. laccei.org. 2023;. Link: laccei.org
-
Rowart, P, et al. (2023) Fast and Efficient Isolation of Exosomes from Stem Cells Using a Combination of Single-Use Bioreactors, High-Speed-and Ultracentrifugation. eppendorf.com. 2023;. Link: eppendorf.com
-
Otahal, A, et al. (2023) Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy. Methods in molecular biology (Clifton, N.J.). 2023; 2598:123-140. PM ID: 36355289
-
Guo, Q, et al. (2023) Glioblastoma upregulates SUMOylation of hnRNP A2/B1 to eliminate the tumor suppressor miR-204-3p, accelerating angiogenesis under hypoxia. Cell death & disease. 2023; 14(2):147. PM ID: 36810326
- See More