EXOCET Exosome Quantitation Kit

Find out how many extracellular vesicles you have in just 20 minutes with this antibody-free, colorimetric assay
  • Fast—complete in 20 minutes
  • Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
  • Quantitative—included calibration standards enable calculation of the number of exosome particles
  • Flexible—works with all mammalian species tested (human, mouse, rat)
  • Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOCET96A-1 EXOCET Exosome Quantitation Kit 96 Reactions $453
- +

Overview

Overview

Move quickly with SBI’s fastest exosome quantitation method

With the EXOCET Exosome Quantitation Kit, you can find out just how many exosomes you’ve isolated in as little as 20 minutes. This antibody-free, colorimetric assay (OD405) measures the activity of Acetyl-CoA Acetylcholinesterase (AChE), an enzyme shown to be highly enriched in exosomes1, 2 (using mass spectrometry and Western blotting, SBI has verified the presence of AChE in exosomes from serum, stem cells, cancer cell lines, and MSCs—contact tech@systembio.com to learn more).

EXOCET is a highly quantitative assay and the standards included with each kit have been calibrated using NanoSight analysis, enabling calculation of the number of exosomes in your samples. The EXOCET Kit contains all of the necessary assay reagents and an optical 96-well plate to perform 96 reactions.

  • Fast—complete in 20 minutes
  • Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
  • Quantitative—included calibration standards enable calculation of the number of exosome particles
  • Flexible—works with all mammalian species tested (human, mouse, rat)
  • Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
Choose the exosome quantitation method that’s best for your studies
 ExoELISA-ULTRA Complete KitsExoELISA KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor sensitive quantitation of exosomes when time and input sample are not limitingFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)Enzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)24 hours20 min60 min
Input sample amount (protein equivalent)1 – 200 µg>500 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCETFluoroCet
REFERENCES
  1. Savina A, et al. The exosome pathway in K562 cells is regulated by Rab11. J Cell Sci. 2002 Jun 15. 115(Pt 12):2505-15. PMID: 12045221.
  2. Gupta S, and Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am J Physiol Heart Circ Physiol. 2007 Jun. 292(6):H3052-6. PMID: 17307989.

How It Works

How It Works

Exosome quantitation with EXOCET is quick and easy

  1. Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
  2. Lyse exosomes with the included Exosome Lysis Buffer
  3. Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm

Supporting Data

Supporting Data

The fast, highly quantitative EXOCET exosome quantitation assay

Sample data and standard curve for EXOCET.

Exosome quantitation with EXOCET takes as little as 20 minutes and 50 µg (protein equivalent) of sample. Exosomes were isolated from either human serum (0.5 mL) or from MDA-MB-231 culture media (10 mL) using standard ExoQuick and ExoQuick-TC reagents. Exosome pellets were resuspended in PBS and total protein concentration measured using a BCA assay (~108 exosomes, 2 µg/µL). Exosomes were lysed using the EXOCET gentle lysis solution to preserve the enzymatic activity of the exosomal AChE enzyme. The standard curve was generated using known numbers of exosomes (as measured by NanoSight) and calibrated with a recombinant AChE enzyme standard solution provided in the kit.

Resources

Citations

  • Zhou, Y, et al. (2022) Effects of Exosomes Derived from Dermal Papilla Cells on Hair Follicle Stem Cells and Hair Follicle Organoids. Alternatives to Animal Testing and Experimentation. 1970 Jan 1; 27(1):1-13. Link: Alternatives to Animal Testing and Experimentation
  • Ilaltdinov, AW. (2022) Exosome-and MicroRNA-Based Therapeutic Approach for Tendinopathy. Thesis. 1970 Jan 1;. Link: Thesis
  • Gandhi, J, et al. (2022) Proteomic profiling of aspergillus flavus endophthalmitis derived extracellular vesicles in an in-vivo murine model. Medical mycology. 1970 Jan 1;. PM ID: 36002004
  • Rudraprasad, D, Naik, MN & Joseph, J. (2022) Proteome profiling of Extracellular Vesicles in Pseudomonas aeruginosa endophthalmitis: Prognostic and therapeutic significance in a mouse model. Experimental cell research. 1970 Jan 1; 419(1):113306. PM ID: 35963322
  • Zheng, Y, et al. (2022) Cancer-derived exosomal circ_0038138 enhances glycolysis, growth, and metastasis of gastric adenocarcinoma via the miR-198/EZH2 axis. Translational oncology. 1970 Jan 1; 25:101479. PM ID: 35987088
  • Heale, K. (2022) A Biophysical and Molecular Characterization of Human Embryonic Stem Cell-Derived Exosomes. Thesis. 1970 Jan 1;. Link: Thesis
  • Rudraprasad, D, et al. (2022) Characterization and proteome profiling of extracellular vesicles in a murine model of Staphylococcus aureus endophthalmitis. Microbes and infection. 1970 Jan 1;:105014. PM ID: 35609864
  • Anita, L, et al. (2022) Pericyte-derived extracellular vesicle-mimetic nanovesicles ameliorate erectile dysfunction via lipocalin 2 in diabetic mice. International Journal of Biological Sciences. 1970 Jan 1; 18(9):3653-3667. Link: International Journal of Biological Sciences
  • Durur, DY, et al. (2022) Alteration of miRNAs in Small Neuron-Derived Extracellular Vesicles of Alzheimer’s Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses. Journal of molecular neuroscience : MN. 1970 Jan 1;. PM ID: 35488079
  • Yin, GN, et al. (2022) Pericyte‑derived extracellular vesicles‑mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection. International journal of molecular medicine. 1970 Jan 1; 49(2). PM ID: 34935051
  • Hajipour, H, et al. (2021) A human chorionic gonadotropin (hCG) delivery platform using engineered uterine exosomes to improve endometrial receptivity. Life sciences. 1970 Jan 1; 275:119351. PM ID: 33737084
  • Wu, Z, He, D & Li, H. (2021) Bioglass enhances the production of exosomes and improves their capability of promoting vascularization. Bioactive Materials. 1970 Jan 1; 6(3):823-835. Link: Bioactive Materials
  • Ito, T, et al. (2021) Microbial Antigen-Presenting Extracellular Vesicles Derived from Genetically Modified Tumor Cells Promote Antitumor Activity of Dendritic Cells. Pharmaceutics. 1970 Jan 1; 13(1). PM ID: 33406722
  • Kim, MH, Kim, D & Sung, JH. (2021) A Gut-Brain Axis-on-a-Chip for studying transport across epithelial and endothelial barriers. Authorea Preprints. 1970 Jan 1;. Link: Authorea Preprints
  • Zhang, L, et al. (2021) Small extracellular vesicles control dendritic spine development through regulation of HDAC2 signaling. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1970 Jan 1;. PM ID: 33741723
  • Kwon, S, et al. (2021) Enhanced Diamagnetic Repulsion of Blood Cells Enables Versatile Plasma Separation for Biomarker Analysis in Blood. Small (Weinheim an der Bergstrasse, Germany). 1970 Jan 1;:e2100797. PM ID: 33978996
  • Bozorgmehr, N, et al. (2021) Expanded antigen-experienced CD160+CD8+effector T cells exhibit impaired effector functions in chronic lymphocytic leukemia. Journal for immunotherapy of cancer. 1970 Jan 1; 9(4). PM ID: 33931471
  • Huang, H, et al. (2021) RAB27A-dependent release of exosomes by liver cancer stem cells induces Nanog expression in their differentiated progenies and confers Regorafenib resistance. Journal of gastroenterology and hepatology. 1970 Jan 1;. PM ID: 34258777
  • Yin, GN, et al. (2020) Pericyte-Derived Extracellular Vesicle-Mimetic Nanovesicles Restore Erectile Function by Enhancing Neurovascular Regeneration in a Mouse Model of Cavernous Nerve Injury. J Sex Med. 1970 Jan 1;. PM ID: 32855091
  • Feng, Y, et al. (2020) Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease. Cell Death Dis. 1970 Jan 1; 11(7):513. PM ID: 32641688

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOCET96A-1 EXOCET Exosome Quantitation Kit 96 Reactions $453
- +

Overview

Overview

Move quickly with SBI’s fastest exosome quantitation method

With the EXOCET Exosome Quantitation Kit, you can find out just how many exosomes you’ve isolated in as little as 20 minutes. This antibody-free, colorimetric assay (OD405) measures the activity of Acetyl-CoA Acetylcholinesterase (AChE), an enzyme shown to be highly enriched in exosomes1, 2 (using mass spectrometry and Western blotting, SBI has verified the presence of AChE in exosomes from serum, stem cells, cancer cell lines, and MSCs—contact tech@systembio.com to learn more).

EXOCET is a highly quantitative assay and the standards included with each kit have been calibrated using NanoSight analysis, enabling calculation of the number of exosomes in your samples. The EXOCET Kit contains all of the necessary assay reagents and an optical 96-well plate to perform 96 reactions.

  • Fast—complete in 20 minutes
  • Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
  • Quantitative—included calibration standards enable calculation of the number of exosome particles
  • Flexible—works with all mammalian species tested (human, mouse, rat)
  • Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
Choose the exosome quantitation method that’s best for your studies
 ExoELISA-ULTRA Complete KitsExoELISA KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor sensitive quantitation of exosomes when time and input sample are not limitingFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)Enzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)24 hours20 min60 min
Input sample amount (protein equivalent)1 – 200 µg>500 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCETFluoroCet
REFERENCES
  1. Savina A, et al. The exosome pathway in K562 cells is regulated by Rab11. J Cell Sci. 2002 Jun 15. 115(Pt 12):2505-15. PMID: 12045221.
  2. Gupta S, and Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am J Physiol Heart Circ Physiol. 2007 Jun. 292(6):H3052-6. PMID: 17307989.

How It Works

How It Works

Exosome quantitation with EXOCET is quick and easy

  1. Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
  2. Lyse exosomes with the included Exosome Lysis Buffer
  3. Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm

Supporting Data

Supporting Data

The fast, highly quantitative EXOCET exosome quantitation assay

Sample data and standard curve for EXOCET.

Exosome quantitation with EXOCET takes as little as 20 minutes and 50 µg (protein equivalent) of sample. Exosomes were isolated from either human serum (0.5 mL) or from MDA-MB-231 culture media (10 mL) using standard ExoQuick and ExoQuick-TC reagents. Exosome pellets were resuspended in PBS and total protein concentration measured using a BCA assay (~108 exosomes, 2 µg/µL). Exosomes were lysed using the EXOCET gentle lysis solution to preserve the enzymatic activity of the exosomal AChE enzyme. The standard curve was generated using known numbers of exosomes (as measured by NanoSight) and calibrated with a recombinant AChE enzyme standard solution provided in the kit.

Citations

  • Zhou, Y, et al. (2022) Effects of Exosomes Derived from Dermal Papilla Cells on Hair Follicle Stem Cells and Hair Follicle Organoids. Alternatives to Animal Testing and Experimentation. 1970 Jan 1; 27(1):1-13. Link: Alternatives to Animal Testing and Experimentation
  • Ilaltdinov, AW. (2022) Exosome-and MicroRNA-Based Therapeutic Approach for Tendinopathy. Thesis. 1970 Jan 1;. Link: Thesis
  • Gandhi, J, et al. (2022) Proteomic profiling of aspergillus flavus endophthalmitis derived extracellular vesicles in an in-vivo murine model. Medical mycology. 1970 Jan 1;. PM ID: 36002004
  • Rudraprasad, D, Naik, MN & Joseph, J. (2022) Proteome profiling of Extracellular Vesicles in Pseudomonas aeruginosa endophthalmitis: Prognostic and therapeutic significance in a mouse model. Experimental cell research. 1970 Jan 1; 419(1):113306. PM ID: 35963322
  • Zheng, Y, et al. (2022) Cancer-derived exosomal circ_0038138 enhances glycolysis, growth, and metastasis of gastric adenocarcinoma via the miR-198/EZH2 axis. Translational oncology. 1970 Jan 1; 25:101479. PM ID: 35987088
  • Heale, K. (2022) A Biophysical and Molecular Characterization of Human Embryonic Stem Cell-Derived Exosomes. Thesis. 1970 Jan 1;. Link: Thesis
  • Rudraprasad, D, et al. (2022) Characterization and proteome profiling of extracellular vesicles in a murine model of Staphylococcus aureus endophthalmitis. Microbes and infection. 1970 Jan 1;:105014. PM ID: 35609864
  • Anita, L, et al. (2022) Pericyte-derived extracellular vesicle-mimetic nanovesicles ameliorate erectile dysfunction via lipocalin 2 in diabetic mice. International Journal of Biological Sciences. 1970 Jan 1; 18(9):3653-3667. Link: International Journal of Biological Sciences
  • Durur, DY, et al. (2022) Alteration of miRNAs in Small Neuron-Derived Extracellular Vesicles of Alzheimer’s Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses. Journal of molecular neuroscience : MN. 1970 Jan 1;. PM ID: 35488079
  • Yin, GN, et al. (2022) Pericyte‑derived extracellular vesicles‑mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection. International journal of molecular medicine. 1970 Jan 1; 49(2). PM ID: 34935051
  • Hajipour, H, et al. (2021) A human chorionic gonadotropin (hCG) delivery platform using engineered uterine exosomes to improve endometrial receptivity. Life sciences. 1970 Jan 1; 275:119351. PM ID: 33737084
  • Wu, Z, He, D & Li, H. (2021) Bioglass enhances the production of exosomes and improves their capability of promoting vascularization. Bioactive Materials. 1970 Jan 1; 6(3):823-835. Link: Bioactive Materials
  • Ito, T, et al. (2021) Microbial Antigen-Presenting Extracellular Vesicles Derived from Genetically Modified Tumor Cells Promote Antitumor Activity of Dendritic Cells. Pharmaceutics. 1970 Jan 1; 13(1). PM ID: 33406722
  • Kim, MH, Kim, D & Sung, JH. (2021) A Gut-Brain Axis-on-a-Chip for studying transport across epithelial and endothelial barriers. Authorea Preprints. 1970 Jan 1;. Link: Authorea Preprints
  • Zhang, L, et al. (2021) Small extracellular vesicles control dendritic spine development through regulation of HDAC2 signaling. The Journal of neuroscience : the official journal of the Society for Neuroscience. 1970 Jan 1;. PM ID: 33741723
  • Kwon, S, et al. (2021) Enhanced Diamagnetic Repulsion of Blood Cells Enables Versatile Plasma Separation for Biomarker Analysis in Blood. Small (Weinheim an der Bergstrasse, Germany). 1970 Jan 1;:e2100797. PM ID: 33978996
  • Bozorgmehr, N, et al. (2021) Expanded antigen-experienced CD160+CD8+effector T cells exhibit impaired effector functions in chronic lymphocytic leukemia. Journal for immunotherapy of cancer. 1970 Jan 1; 9(4). PM ID: 33931471
  • Huang, H, et al. (2021) RAB27A-dependent release of exosomes by liver cancer stem cells induces Nanog expression in their differentiated progenies and confers Regorafenib resistance. Journal of gastroenterology and hepatology. 1970 Jan 1;. PM ID: 34258777
  • Yin, GN, et al. (2020) Pericyte-Derived Extracellular Vesicle-Mimetic Nanovesicles Restore Erectile Function by Enhancing Neurovascular Regeneration in a Mouse Model of Cavernous Nerve Injury. J Sex Med. 1970 Jan 1;. PM ID: 32855091
  • Feng, Y, et al. (2020) Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease. Cell Death Dis. 1970 Jan 1; 11(7):513. PM ID: 32641688