EXOCET Standards
- Fast—complete in 20 minutes
- Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
- Quantitative—included calibration standards enable calculation of the number of exosome particles
- Flexible—works with all mammalian species tested (human, mouse, rat)
- Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXOCET-SD-1 | EXOCET Standards | 1 Set | $155 |
|
Overview
Overview
Extra standards for EXOCETWhile our fast and easy EXOCET Kit comes with standards, we also offer our EXOCET exosome standards as a stand-alone product for when you need more. The standards are calibrated using NanoSight analysis, enabling estimation of the number of exosomes in your samples.
The EXOCET Exosome Quantitation Kit is:
- Fast—complete in 20 minutes
- Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
- Quantitative—included calibration standards enable calculation of the number of exosome particles
- Flexible—works with all mammalian species tested (human, mouse, rat)
- Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
---|---|---|---|
Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
Detection method | Antibody | Enzymatic | Enzymatic |
Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
ExoELISA-ULTRA CD63 Detection | ExoELISA CD9 ExoELISA CD63 ExoELISA CD81 | EXOCET | FluoroCet | |
Use | For fast and sensitive antibody-based quantitation of exosomes | For sensitive quantitation of exosomes when time and input sample are not limiting | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
Detection method | Antibody | Antibody | Enzymatic | Enzymatic |
Quantitation chemistry | Enzymatic (HRP) | Enzymatic (HRP) | Colorimetric | Fluorescent |
Total protocol time | 4 hours (no overnight incubation) | 24 hours | 20 min | 60 min |
Input sample amount (protein equivalent) | 1 – 200 µg | >500 µg | 50 µg | <1 µg |
How It Works
How It Works
Exosome quantitation with EXOCET is quick and easy
- Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
- Lyse exosomes with the included Exosome Lysis Buffer
- Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm
Supporting Data
FAQs
Resources
Related Products
Citations
-
Irep, N, et al. (2024) Exosome inhibition improves response to first-line therapy in small cell lung cancer. Journal of cellular and molecular medicine. 2024; 28(4):e18138. PM ID: 38353469
-
Karacam, B, et al. (2024) Role of cell-free DNA and extracellular vesicles for diagnosis and surveillance in patients with glioma. The Journal of Liquid Biopsy. 2024; 4:100142. Link: The Journal of Liquid Biopsy
-
Ock, J, et al. (2023) Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injury. International Journal of Biological Sciences. 2023; 19(9):2663-2677. Link: International Journal of Biological Sciences
-
Yang, Z, et al. (2023) Human umbilical cord mesenchymal stem cell-derived extracellular vesicles loaded with TFCP2 activate Wnt/β-catenin signaling to alleviate preeclampsia. International Immunopharmacology. 2023; 115:109732. Link: International Immunopharmacology
-
Graça, AL, et al. (2023) Platelet-Derived Extracellular Vesicles Promote Tenogenic Differentiation of Stem Cells on Bioengineered Living Fibers. International journal of molecular sciences. 2023; 24(4). PM ID: 36834925
-
Wang, X, et al. (2023) RIP1 Mediates Manzamine-A-Induced Secretory Autophagy in Breast Cancer. Marine Drugs. 2023; 21(3):151. Link: Marine Drugs
-
Kawanishi, N, Tominaga, T & Suzuki, K. (2023) Electrical pulse stimulation-induced muscle contraction alters the microRNA and mRNA profiles of circulating extracellular vesicles in mice. American journal of physiology. Regulatory, integrative and comparative physiology. 2023;. PM ID: 37092746
-
Rodríguez‐Comas, J, et al. (2023) Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma. Advanced Materials Technologies. 2023;. Link: Advanced Materials Technologies
-
Arcuri, S, et al. (2023) 3D ECM-Based Scaffolds Boost Young Cell Secretome-Derived EV Rejuvenating Effects in Senescent Cells. International journal of molecular sciences. 2023; 24(9). PM ID: 37175996
-
WINSTON, T. (2023) Targeted mesenchymal stem cell differentiation from induced pluripotent stem cells for therapeutic application. Thesis. 2023;. Link: Thesis
-
Pedrosa, M, et al. (2023) Extracellular Vesicles and Their Renin-Angiotensin Cargo as a Link between Metabolic Syndrome and Parkinson’s Disease. Antioxidants. 2023; 12(12):2045. Link: Antioxidants
-
Sun, T, et al. (2023) Novel 3D-printing bilayer GelMA-based hydrogel containing BP,β-TCP and exosomes for cartilage-bone integrated repair. Biofabrication. 2023; 16(1). PM ID: 37857284
-
Jung, YK & Son, MH. (2023) Polydiacetylene-based aptasensors for rapid and specific colorimetric detection of malignant exosomes. Talanta. 2023; 268(Pt 1):125342. PM ID: 37918246
-
Toledo-Guardiola, SM, et al. (2023) Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig. Frontiers in cell and developmental biology. 2023; 11:1231755. PM ID: 37868907
-
Ilaltdinov, AW. (2022) Exosome-and MicroRNA-Based Therapeutic Approach for Tendinopathy. Thesis. 2022;. Link: Thesis
-
Yin, GN, et al. (2022) Pericyte‑derived extracellular vesicles‑mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection. International journal of molecular medicine. 2022; 49(2). PM ID: 34935051
-
Durur, DY, et al. (2022) Alteration of miRNAs in Small Neuron-Derived Extracellular Vesicles of Alzheimer’s Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses. Journal of molecular neuroscience : MN. 2022;. PM ID: 35488079
-
Anita, L, et al. (2022) Pericyte-derived extracellular vesicle-mimetic nanovesicles ameliorate erectile dysfunction via lipocalin 2 in diabetic mice. International Journal of Biological Sciences. 2022; 18(9):3653-3667. Link: International Journal of Biological Sciences
-
Rudraprasad, D, et al. (2022) Characterization and proteome profiling of extracellular vesicles in a murine model of Staphylococcus aureus endophthalmitis. Microbes and infection. 2022;:105014. PM ID: 35609864
-
Heale, K. (2022) A Biophysical and Molecular Characterization of Human Embryonic Stem Cell-Derived Exosomes. Thesis. 2022;. Link: Thesis
- See More
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
EXOCET-SD-1 | EXOCET Standards | 1 Set | $155 |
|
Overview
Overview
Extra standards for EXOCETWhile our fast and easy EXOCET Kit comes with standards, we also offer our EXOCET exosome standards as a stand-alone product for when you need more. The standards are calibrated using NanoSight analysis, enabling estimation of the number of exosomes in your samples.
The EXOCET Exosome Quantitation Kit is:
- Fast—complete in 20 minutes
- Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
- Quantitative—included calibration standards enable calculation of the number of exosome particles
- Flexible—works with all mammalian species tested (human, mouse, rat)
- Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
ExoELISA-ULTRA Complete Kits | EXOCET | FluoroCet | |
---|---|---|---|
Use | For fast and sensitive antibody-based quantitation of exosomes | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
Detection method | Antibody | Enzymatic | Enzymatic |
Quantitation chemistry | Enzymatic (HRP) | Colorimetric | Fluorescent |
Total protocol time | 4 hours (no overnight incubation) | 20 min | 60 min |
Input sample amount (protein equivalent) | 1 – 200 µg | 50 µg | <1 µg |
Learn More | ExoELISA-ULTRA CD63 ExoELISA-ULTRA CD81 ExoELISA-ULTRA CD9 | EXOCET | FluoroCet |
ExoELISA-ULTRA CD63 Detection | ExoELISA CD9 ExoELISA CD63 ExoELISA CD81 | EXOCET | FluoroCet | |
Use | For fast and sensitive antibody-based quantitation of exosomes | For sensitive quantitation of exosomes when time and input sample are not limiting | For fast quantitation of extracellular vesicles with moderate sample input requirements | For the most sensitive quantitation of extracellular vesicles with very low sample input requirements |
Detection method | Antibody | Antibody | Enzymatic | Enzymatic |
Quantitation chemistry | Enzymatic (HRP) | Enzymatic (HRP) | Colorimetric | Fluorescent |
Total protocol time | 4 hours (no overnight incubation) | 24 hours | 20 min | 60 min |
Input sample amount (protein equivalent) | 1 – 200 µg | >500 µg | 50 µg | <1 µg |
How It Works
How It Works
Exosome quantitation with EXOCET is quick and easy
- Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
- Lyse exosomes with the included Exosome Lysis Buffer
- Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm
FAQs
Citations
-
Irep, N, et al. (2024) Exosome inhibition improves response to first-line therapy in small cell lung cancer. Journal of cellular and molecular medicine. 2024; 28(4):e18138. PM ID: 38353469
-
Karacam, B, et al. (2024) Role of cell-free DNA and extracellular vesicles for diagnosis and surveillance in patients with glioma. The Journal of Liquid Biopsy. 2024; 4:100142. Link: The Journal of Liquid Biopsy
-
Ock, J, et al. (2023) Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injury. International Journal of Biological Sciences. 2023; 19(9):2663-2677. Link: International Journal of Biological Sciences
-
Yang, Z, et al. (2023) Human umbilical cord mesenchymal stem cell-derived extracellular vesicles loaded with TFCP2 activate Wnt/β-catenin signaling to alleviate preeclampsia. International Immunopharmacology. 2023; 115:109732. Link: International Immunopharmacology
-
Graça, AL, et al. (2023) Platelet-Derived Extracellular Vesicles Promote Tenogenic Differentiation of Stem Cells on Bioengineered Living Fibers. International journal of molecular sciences. 2023; 24(4). PM ID: 36834925
-
Wang, X, et al. (2023) RIP1 Mediates Manzamine-A-Induced Secretory Autophagy in Breast Cancer. Marine Drugs. 2023; 21(3):151. Link: Marine Drugs
-
Kawanishi, N, Tominaga, T & Suzuki, K. (2023) Electrical pulse stimulation-induced muscle contraction alters the microRNA and mRNA profiles of circulating extracellular vesicles in mice. American journal of physiology. Regulatory, integrative and comparative physiology. 2023;. PM ID: 37092746
-
Rodríguez‐Comas, J, et al. (2023) Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma. Advanced Materials Technologies. 2023;. Link: Advanced Materials Technologies
-
Arcuri, S, et al. (2023) 3D ECM-Based Scaffolds Boost Young Cell Secretome-Derived EV Rejuvenating Effects in Senescent Cells. International journal of molecular sciences. 2023; 24(9). PM ID: 37175996
-
WINSTON, T. (2023) Targeted mesenchymal stem cell differentiation from induced pluripotent stem cells for therapeutic application. Thesis. 2023;. Link: Thesis
-
Pedrosa, M, et al. (2023) Extracellular Vesicles and Their Renin-Angiotensin Cargo as a Link between Metabolic Syndrome and Parkinson’s Disease. Antioxidants. 2023; 12(12):2045. Link: Antioxidants
-
Sun, T, et al. (2023) Novel 3D-printing bilayer GelMA-based hydrogel containing BP,β-TCP and exosomes for cartilage-bone integrated repair. Biofabrication. 2023; 16(1). PM ID: 37857284
-
Jung, YK & Son, MH. (2023) Polydiacetylene-based aptasensors for rapid and specific colorimetric detection of malignant exosomes. Talanta. 2023; 268(Pt 1):125342. PM ID: 37918246
-
Toledo-Guardiola, SM, et al. (2023) Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig. Frontiers in cell and developmental biology. 2023; 11:1231755. PM ID: 37868907
-
Ilaltdinov, AW. (2022) Exosome-and MicroRNA-Based Therapeutic Approach for Tendinopathy. Thesis. 2022;. Link: Thesis
-
Yin, GN, et al. (2022) Pericyte‑derived extracellular vesicles‑mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection. International journal of molecular medicine. 2022; 49(2). PM ID: 34935051
-
Durur, DY, et al. (2022) Alteration of miRNAs in Small Neuron-Derived Extracellular Vesicles of Alzheimer’s Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses. Journal of molecular neuroscience : MN. 2022;. PM ID: 35488079
-
Anita, L, et al. (2022) Pericyte-derived extracellular vesicle-mimetic nanovesicles ameliorate erectile dysfunction via lipocalin 2 in diabetic mice. International Journal of Biological Sciences. 2022; 18(9):3653-3667. Link: International Journal of Biological Sciences
-
Rudraprasad, D, et al. (2022) Characterization and proteome profiling of extracellular vesicles in a murine model of Staphylococcus aureus endophthalmitis. Microbes and infection. 2022;:105014. PM ID: 35609864
-
Heale, K. (2022) A Biophysical and Molecular Characterization of Human Embryonic Stem Cell-Derived Exosomes. Thesis. 2022;. Link: Thesis
- See More