EXOCET Exosome Quantitation Kit

Find out how many extracellular vesicles you have in just 20 minutes with this antibody-free, colorimetric assay
  • Fast—complete in 20 minutes
  • Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
  • Quantitative—included calibration standards enable calculation of the number of exosome particles
  • Flexible—works with all mammalian species tested (human, mouse, rat)
  • Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOCET96A-1 EXOCET Exosome Quantitation Kit 96 Reactions $479
- +

Overview

Overview

Move quickly with SBI’s fastest exosome quantitation method

With the EXOCET Exosome Quantitation Kit, you can find out just how many exosomes you’ve isolated in as little as 20 minutes. This antibody-free, colorimetric assay (OD405) measures the activity of Acetyl-CoA Acetylcholinesterase (AChE), an enzyme shown to be highly enriched in exosomes1, 2 (using mass spectrometry and Western blotting, SBI has verified the presence of AChE in exosomes from serum, stem cells, cancer cell lines, and MSCs—contact tech@systembio.com to learn more).

EXOCET is a highly quantitative assay and the standards included with each kit have been calibrated using NanoSight analysis, enabling calculation of the number of exosomes in your samples. The EXOCET Kit contains all of the necessary assay reagents and an optical 96-well plate to perform 96 reactions.

  • Fast—complete in 20 minutes
  • Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
  • Quantitative—included calibration standards enable calculation of the number of exosome particles
  • Flexible—works with all mammalian species tested (human, mouse, rat)
  • Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
Choose the exosome quantitation method that’s best for your studies
ExoELISA-ULTRA Complete KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)20 min60 min
Input sample amount (protein equivalent)1 – 200 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
EXOCETFluoroCet
REFERENCES
  1. Savina A, et al. The exosome pathway in K562 cells is regulated by Rab11. J Cell Sci. 2002 Jun 15. 115(Pt 12):2505-15. PMID: 12045221.
  2. Gupta S, and Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am J Physiol Heart Circ Physiol. 2007 Jun. 292(6):H3052-6. PMID: 17307989.

How It Works

How It Works

Exosome quantitation with EXOCET is quick and easy

  1. Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
  2. Lyse exosomes with the included Exosome Lysis Buffer
  3. Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm

Supporting Data

Supporting Data

The fast, highly quantitative EXOCET exosome quantitation assay

Sample data and standard curve for EXOCET.

Exosome quantitation with EXOCET takes as little as 20 minutes and 50 µg (protein equivalent) of sample. Exosomes were isolated from either human serum (0.5 mL) or from MDA-MB-231 culture media (10 mL) using standard ExoQuick and ExoQuick-TC reagents. Exosome pellets were resuspended in PBS and total protein concentration measured using a BCA assay (~108 exosomes, 2 µg/µL). Exosomes were lysed using the EXOCET gentle lysis solution to preserve the enzymatic activity of the exosomal AChE enzyme. The standard curve was generated using known numbers of exosomes (as measured by NanoSight) and calibrated with a recombinant AChE enzyme standard solution provided in the kit.

FAQs

Resources

Citations

  • Wang, M, et al. (2024) A tale of two tumors: differential, but detrimental, effects of glioblastoma extracellular vesicles (EVs) on normal human brain cells. bioRxiv : the preprint server for biology. 2024;. PM ID: 38645117
  • Thangavel, H, et al. (2024) Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation. iScience. 2024; 27(5):109672. PM ID: 38660407
  • Irep, N, et al. (2024) Exosome inhibition improves response to first-line therapy in small cell lung cancer. Journal of cellular and molecular medicine. 2024; 28(4):e18138. PM ID: 38353469
  • Karacam, B, et al. (2024) Role of cell-free DNA and extracellular vesicles for diagnosis and surveillance in patients with glioma. The Journal of Liquid Biopsy. 2024; 4:100142. Link: The Journal of Liquid Biopsy
  • Rodríguez‐Comas, J, et al. (2023) Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma. Advanced Materials Technologies. 2023;. Link: Advanced Materials Technologies
  • Yang, Z, et al. (2023) Human umbilical cord mesenchymal stem cell-derived extracellular vesicles loaded with TFCP2 activate Wnt/β-catenin signaling to alleviate preeclampsia. International Immunopharmacology. 2023; 115:109732. Link: International Immunopharmacology
  • Graça, AL, et al. (2023) Platelet-Derived Extracellular Vesicles Promote Tenogenic Differentiation of Stem Cells on Bioengineered Living Fibers. International journal of molecular sciences. 2023; 24(4). PM ID: 36834925
  • Wang, X, et al. (2023) RIP1 Mediates Manzamine-A-Induced Secretory Autophagy in Breast Cancer. Marine Drugs. 2023; 21(3):151. Link: Marine Drugs
  • Kawanishi, N, Tominaga, T & Suzuki, K. (2023) Electrical pulse stimulation-induced muscle contraction alters the microRNA and mRNA profiles of circulating extracellular vesicles in mice. American journal of physiology. Regulatory, integrative and comparative physiology. 2023;. PM ID: 37092746
  • Ock, J, et al. (2023) Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injury. International Journal of Biological Sciences. 2023; 19(9):2663-2677. Link: International Journal of Biological Sciences
  • Arcuri, S, et al. (2023) 3D ECM-Based Scaffolds Boost Young Cell Secretome-Derived EV Rejuvenating Effects in Senescent Cells. International journal of molecular sciences. 2023; 24(9). PM ID: 37175996
  • WINSTON, T. (2023) Targeted mesenchymal stem cell differentiation from induced pluripotent stem cells for therapeutic application. Thesis. 2023;. Link: Thesis
  • Pedrosa, M, et al. (2023) Extracellular Vesicles and Their Renin-Angiotensin Cargo as a Link between Metabolic Syndrome and Parkinson’s Disease. Antioxidants. 2023; 12(12):2045. Link: Antioxidants
  • Sun, T, et al. (2023) Novel 3D-printing bilayer GelMA-based hydrogel containing BP,β-TCP and exosomes for cartilage-bone integrated repair. Biofabrication. 2023; 16(1). PM ID: 37857284
  • Jung, YK & Son, MH. (2023) Polydiacetylene-based aptasensors for rapid and specific colorimetric detection of malignant exosomes. Talanta. 2023; 268(Pt 1):125342. PM ID: 37918246
  • Toledo-Guardiola, SM, et al. (2023) Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig. Frontiers in cell and developmental biology. 2023; 11:1231755. PM ID: 37868907
  • Liao, XM, et al. (2022) Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells. BMC oral health. 2022; 22(1):647. PM ID: 36575449
  • Yin, GN, et al. (2022) Pericyte‑derived extracellular vesicles‑mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection. International journal of molecular medicine. 2022; 49(2). PM ID: 34935051
  • Durur, DY, et al. (2022) Alteration of miRNAs in Small Neuron-Derived Extracellular Vesicles of Alzheimer’s Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses. Journal of molecular neuroscience : MN. 2022;. PM ID: 35488079
  • Anita, L, et al. (2022) Pericyte-derived extracellular vesicle-mimetic nanovesicles ameliorate erectile dysfunction via lipocalin 2 in diabetic mice. International Journal of Biological Sciences. 2022; 18(9):3653-3667. Link: International Journal of Biological Sciences

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOCET96A-1 EXOCET Exosome Quantitation Kit 96 Reactions $479
- +

Overview

Overview

Move quickly with SBI’s fastest exosome quantitation method

With the EXOCET Exosome Quantitation Kit, you can find out just how many exosomes you’ve isolated in as little as 20 minutes. This antibody-free, colorimetric assay (OD405) measures the activity of Acetyl-CoA Acetylcholinesterase (AChE), an enzyme shown to be highly enriched in exosomes1, 2 (using mass spectrometry and Western blotting, SBI has verified the presence of AChE in exosomes from serum, stem cells, cancer cell lines, and MSCs—contact tech@systembio.com to learn more).

EXOCET is a highly quantitative assay and the standards included with each kit have been calibrated using NanoSight analysis, enabling calculation of the number of exosomes in your samples. The EXOCET Kit contains all of the necessary assay reagents and an optical 96-well plate to perform 96 reactions.

  • Fast—complete in 20 minutes
  • Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
  • Quantitative—included calibration standards enable calculation of the number of exosome particles
  • Flexible—works with all mammalian species tested (human, mouse, rat)
  • Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography
Choose the exosome quantitation method that’s best for your studies
ExoELISA-ULTRA Complete KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)20 min60 min
Input sample amount (protein equivalent)1 – 200 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
EXOCETFluoroCet
REFERENCES
  1. Savina A, et al. The exosome pathway in K562 cells is regulated by Rab11. J Cell Sci. 2002 Jun 15. 115(Pt 12):2505-15. PMID: 12045221.
  2. Gupta S, and Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am J Physiol Heart Circ Physiol. 2007 Jun. 292(6):H3052-6. PMID: 17307989.

How It Works

How It Works

Exosome quantitation with EXOCET is quick and easy

  1. Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
  2. Lyse exosomes with the included Exosome Lysis Buffer
  3. Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm

Supporting Data

Supporting Data

The fast, highly quantitative EXOCET exosome quantitation assay

Sample data and standard curve for EXOCET.

Exosome quantitation with EXOCET takes as little as 20 minutes and 50 µg (protein equivalent) of sample. Exosomes were isolated from either human serum (0.5 mL) or from MDA-MB-231 culture media (10 mL) using standard ExoQuick and ExoQuick-TC reagents. Exosome pellets were resuspended in PBS and total protein concentration measured using a BCA assay (~108 exosomes, 2 µg/µL). Exosomes were lysed using the EXOCET gentle lysis solution to preserve the enzymatic activity of the exosomal AChE enzyme. The standard curve was generated using known numbers of exosomes (as measured by NanoSight) and calibrated with a recombinant AChE enzyme standard solution provided in the kit.

FAQs

Citations

  • Wang, M, et al. (2024) A tale of two tumors: differential, but detrimental, effects of glioblastoma extracellular vesicles (EVs) on normal human brain cells. bioRxiv : the preprint server for biology. 2024;. PM ID: 38645117
  • Thangavel, H, et al. (2024) Adipocyte-released adipomes in Chagas cardiomyopathy: Impact on cardiac metabolic and immune regulation. iScience. 2024; 27(5):109672. PM ID: 38660407
  • Irep, N, et al. (2024) Exosome inhibition improves response to first-line therapy in small cell lung cancer. Journal of cellular and molecular medicine. 2024; 28(4):e18138. PM ID: 38353469
  • Karacam, B, et al. (2024) Role of cell-free DNA and extracellular vesicles for diagnosis and surveillance in patients with glioma. The Journal of Liquid Biopsy. 2024; 4:100142. Link: The Journal of Liquid Biopsy
  • Rodríguez‐Comas, J, et al. (2023) Immunoaffinity‐Based Microfluidic Platform for Exosomal MicroRNA Isolation from Obese and Lean Mouse Plasma. Advanced Materials Technologies. 2023;. Link: Advanced Materials Technologies
  • Yang, Z, et al. (2023) Human umbilical cord mesenchymal stem cell-derived extracellular vesicles loaded with TFCP2 activate Wnt/β-catenin signaling to alleviate preeclampsia. International Immunopharmacology. 2023; 115:109732. Link: International Immunopharmacology
  • Graça, AL, et al. (2023) Platelet-Derived Extracellular Vesicles Promote Tenogenic Differentiation of Stem Cells on Bioengineered Living Fibers. International journal of molecular sciences. 2023; 24(4). PM ID: 36834925
  • Wang, X, et al. (2023) RIP1 Mediates Manzamine-A-Induced Secretory Autophagy in Breast Cancer. Marine Drugs. 2023; 21(3):151. Link: Marine Drugs
  • Kawanishi, N, Tominaga, T & Suzuki, K. (2023) Electrical pulse stimulation-induced muscle contraction alters the microRNA and mRNA profiles of circulating extracellular vesicles in mice. American journal of physiology. Regulatory, integrative and comparative physiology. 2023;. PM ID: 37092746
  • Ock, J, et al. (2023) Heme-binding protein 1 delivered via pericyte-derived extracellular vesicles improves neurovascular regeneration in a mouse model of cavernous nerve injury. International Journal of Biological Sciences. 2023; 19(9):2663-2677. Link: International Journal of Biological Sciences
  • Arcuri, S, et al. (2023) 3D ECM-Based Scaffolds Boost Young Cell Secretome-Derived EV Rejuvenating Effects in Senescent Cells. International journal of molecular sciences. 2023; 24(9). PM ID: 37175996
  • WINSTON, T. (2023) Targeted mesenchymal stem cell differentiation from induced pluripotent stem cells for therapeutic application. Thesis. 2023;. Link: Thesis
  • Pedrosa, M, et al. (2023) Extracellular Vesicles and Their Renin-Angiotensin Cargo as a Link between Metabolic Syndrome and Parkinson’s Disease. Antioxidants. 2023; 12(12):2045. Link: Antioxidants
  • Sun, T, et al. (2023) Novel 3D-printing bilayer GelMA-based hydrogel containing BP,β-TCP and exosomes for cartilage-bone integrated repair. Biofabrication. 2023; 16(1). PM ID: 37857284
  • Jung, YK & Son, MH. (2023) Polydiacetylene-based aptasensors for rapid and specific colorimetric detection of malignant exosomes. Talanta. 2023; 268(Pt 1):125342. PM ID: 37918246
  • Toledo-Guardiola, SM, et al. (2023) Different seminal ejaculated fractions in artificial insemination condition the protein cargo of oviductal and uterine extracellular vesicles in pig. Frontiers in cell and developmental biology. 2023; 11:1231755. PM ID: 37868907
  • Liao, XM, et al. (2022) Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells. BMC oral health. 2022; 22(1):647. PM ID: 36575449
  • Yin, GN, et al. (2022) Pericyte‑derived extracellular vesicles‑mimetic nanovesicles improves peripheral nerve regeneration in mouse models of sciatic nerve transection. International journal of molecular medicine. 2022; 49(2). PM ID: 34935051
  • Durur, DY, et al. (2022) Alteration of miRNAs in Small Neuron-Derived Extracellular Vesicles of Alzheimer’s Disease Patients and the Effect of Extracellular Vesicles on Microglial Immune Responses. Journal of molecular neuroscience : MN. 2022;. PM ID: 35488079
  • Anita, L, et al. (2022) Pericyte-derived extracellular vesicle-mimetic nanovesicles ameliorate erectile dysfunction via lipocalin 2 in diabetic mice. International Journal of Biological Sciences. 2022; 18(9):3653-3667. Link: International Journal of Biological Sciences