Custom Stable Reporter Cell Lines

Overview

Get greater insights from in vivo studies with luciferase-labeled cell lines

While SBI already offers a number of ready-to-go reporter cell lines, everyone’s studies are different. Which is why SBI also offers Custom Stable Reporter Cell Line Services that can generate promoter- and/or enhancer-reporter fusions. Save time and effort while leveraging our comprehensive range of technologies and our expert team which has years of experience working with a variety of cell lines, including adherent and suspension cells.

Why choose SBI’s Stable Cell Line Services?

• Save time by having SBI build your custom cell lines
• Leverage our highly-regarded gene expression and delivery technologies
• Accomplish more with our fast turnaround times and end-to-end services
• Turn to the company that other companies turn to—we are a trusted stable cell line manufacturer for many large pharma/biotech companies
• Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Available gene expression and delivery technologies

Viral Systems

• Lentivectors—high titer and reliable
  • Random integration
  • Limited insert size
  • High copy number

• Lentivirus Packaging—reliable gene delivery
  • High and ultra-high titers
  • Efficient transduction
  • Optimized reagents

Non-viral Systems

• PhiC31 Integrase System—one-step single-copy integration
  • Site-specific integration
  • Unlimited insert size
  • Single copy number

• PinPoint Targeted Integration System—excellent for creating isogenic cell lines
  • Site-specific integration
  • Unlimited insert size
  • Single copy number

Not sure what you need or where to begin? Contact the services team to set up a complimentary phone consultation—email services@systembio.com.

How It Works

Ordering Stable Cell Line Services is easy

Step 1. Contact the services team for a quote by emailing services@systembio.com.

Step 2. Send us any necessary materials such as parent cell lines (optional).

Step 3. Receive your stable cell line (turn-around time depends on the complexity of your project).

SBI’s Stable Cell Line Services are a great way to leverage our highly-regarded lentivector, PhiC31 Integrase, and PinPoint Targeted Integration technologies.

Supporting Data

Successful generation of dual luciferase and GFP-labeled NF-κB cell line

SBI Website Content/04_Services Pages/Z_Services Image Files/GFP_induce.jpg, Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI Monitor and quantitatively measure NF-κB transactivation in live cells]

generated using a dual GFP and luciferase-labeled cell line constructed by SBI Monitor and quantitatively measure NF-κB transactivation in live cells" />

Figure 1. Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI—monitor and quantitatively measure NFκB transactivation in live cells. Monitor NF-κB activation in real time with GFP using SBI’s NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line (Cat.# TR860A-1).

Figure 2. Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI—monitor and quantitatively measure NFκB transactivation in live cells. Quantitatively measure NF-κB activation in real time with luciferase using SBI’s NF-κB/293/GFP-Luc Transcriptional Reporter Cell Line (Cat.# TR860A-1).