Custom Overexpression Stable Cell Lines
Reliable, high-quality custom stable cell line generation for overexpression of your protein- or regulatory RNA-of-interest
Overview
Leverage SBI’s viral and non-viral vectors in robust overexpression cell lines
Whether you want constitutive or inducible overexpression of a protein or regulatory RNA, SBI’s services team can create the construct and the cell line. We have access to a range of technologies—including stably integrating viral and non-viral vectors—and an expert team experienced at working with a variety of cell lines, including adherent and suspension cells. The result is hundreds of successfully constructed stable cell lines for highly satisfied customers.
Why choose SBI’s Stable Cell Line Services?
- Save time by having SBI build your custom cell lines
- Leverage our highly-regarded gene expression and delivery technologies
- Accomplish more with our fast turnaround times and end-to-end services
- Turn to the company that other companies turn to—we are a trusted stable cell line manufacturer for many large pharma/biotech companies
- Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility
Available gene expression and delivery technologies
Viral Systems
- Lentivectors—high titer and reliable
- Random integration
- Limited insert size
- High copy number
- Lentivirus Packaging—reliable gene delivery
- High and ultra-high titers
- Efficient transduction
- Optimized reagents
Non-viral Systems
- PhiC31 Integrase System—one-step single-copy integration
- Site-specific integration
- Unlimited insert size
- Single copy number
- PinPoint Targeted Integration System—excellent for creating isogenic cell lines
- Site-specific integration
- Unlimited insert size
- Single copy number
Not sure what you need or where to begin? Contact the services team to set up a complimentary phone consultation—email services@systembio.com.
Supporting Data
Successful generation of stable cell lines at SBI
Example of data generated using cell lines constructed by SBI. (Top row) Stable cell line expressing two genes-of-interest with GFP, RFP, hygromycin, and puromycin selection markers. H2286 adherent cells were grown under puromycin and hygromycin selection. (Bottom row) Stable cell line expressing two separate genes-of-interest with GFP, RFP, hygromycin, and puromycin selection markers. H929 suspension cells were grown under puromycin and hygromycin selection.
