PB-CMV-GreenPuro-H1-MCS shRNA Cloning and Expression Vector

Leverage PiggyBac to produce shRNAs with this vector that co-express GFP and puromycin resistance from the CMV promoter, and shRNA from the H1 promoter
  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers

Products

Catalog Number Description Size Price Quantity Add to Cart
PBSI505A-1 PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector 10 µg $612.00
- +
Contact Us

Overview

Overview

Easy and consistent shRNA delivery and expression Not just for genes, the PiggyBac system is also an excellent choice for reliably producing shRNA. The PB-CMV-GreenPuro-H1-MCS shRNA Cloning and Expression Vector (Cat.# PBSI505A-1) drives production of your shRNA from the strong H1 promoter. The vector also features GFP and puromycin resistance co-expressed from the strong CMV promoter, with co-expression mediated by the T2A element. PB-CMV-GreenPuro-H1-MCS shRNA Cloning and Expression Vector With the PiggyBac Transposon System, you can:
  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
Customer Agreements Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology. For end user license information, see the following: * SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.

How It Works

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

The PiggyBac Transposon System’s cut-and-paste mechanism

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Supporting Data

Resources

Citations

  • Wang, S, et al. (2021) Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion. Cell. 1970 Jan 1;. PM ID: 34133940
  • Ng, YH, et al. (2021) Efficient generation of dopaminergic induced neuronal cells with midbrain characteristics. Stem cell reports. 1970 Jan 1;. PM ID: 34171286
  • Ukaji, T, et al. (2021) Novel knock-in mouse model for the evaluation of the therapeutic efficacy and toxicity of human podoplanin-targeting agents. Cancer science. 1970 Jan 1; 112(6):2299-2313. PM ID: 33735501
  • Ichikawa, M, et al. (2021) Generation of tetracycline-controllable CYP3A4-expressing Caco-2 cells by the piggyBac transposon system. Scientific reports. 1970 Jan 1; 11(1):11670. PM ID: 34083621
  • Sato, Y & Kimura, H. (2021) Dynamic Behavior of Inactive X Chromosome Territory During the Cell Cycle as Revealed by H3K27me3-Specific Intracellular Antibody. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2329:237-247. PM ID: 34085227
  • Kim, JS, Pineda, M & Li, P. (2021) Reconstitution of Morphogen Signaling Gradients in Cultured Cells. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2258:43-56. PM ID: 33340353
  • Thomas, HF, et al. (2021) Temporal dissection of an enhancer cluster reveals distinct temporal and functional contributions of individual elements. Molecular cell. 1970 Jan 1;. PM ID: 33482114
  • Van, MV, Fujimori, T & Bintu, L. (2021) Nanobody-mediated control of gene expression and epigenetic memory. Nature communications. 1970 Jan 1; 12(1):537. PM ID: 33483487
  • Geis, M, et al. (2021) Combinatorial targeting of multiple myeloma by complementing T cell engaging antibody fragments. Communications biology. 1970 Jan 1; 4(1):44. PM ID: 33420283
  • Saijoh, S, et al. (2021) Discovery of a chemical compound that suppresses expression of BEX2, a dormant cancer stem cell-related protein. Biochemical and biophysical research communications. 1970 Jan 1; 537:132-139. PM ID: 33412384
  • Lam, N, Yamanaka, S & Perli, S. (2021) CRISPRi mediated Down regulation of SFPQ Gene Expression in Human induced Pluripotent Stem Cells Results in Massive Cell Death. Research Square. 1970 Jan 1;. Link: Research Square
  • Xiang, K & Bartel, D. (2021) The molecular basis of coupling between poly(A)-tail length and translational efficiency. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Sugimoto, S, et al. (2021) An organoid-based organ-repurposing approach to treat short bowel syndrome. Nature. 1970 Jan 1;. PM ID: 33627870
  • Ma, P, et al. (2021) Avidity‐Based Selection of Tissue‐Specific CAR‐T Cells from a Combinatorial Cellular Library of CARs. Advanced Science. 1970 Jan 1;:2003091. Link: Advanced Science
  • Cho, JH, et al. (2021) Engineering advanced logic and distributed computing in human CAR immune cells. Nature communications. 1970 Jan 1; 12(1):792. PM ID: 33542232
  • Omori, S, et al. (2021) Tim4 recognizes carbon nanotubes and mediates phagocytosis leading to granuloma formation. Cell reports. 1970 Jan 1; 34(6):108734. PM ID: 33567275
  • Tjalsma, SJD, et al. (2021) H4K20me1 and H3K27me3 are concurrently loaded onto the inactive X chromosome but dispensable for inducing gene silencing. EMBO reports. 1970 Jan 1;:e51989. PM ID: 33605056
  • Kitano, H, et al. (2021) Development of a genetically modified hepatoma cell line with heat-inducible high liver function. Cytotechnology. 1970 Jan 1;. Link: Cytotechnology
  • Zhu, R, et al. (2021) Synthetic multistability in mammalian cells. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Karlsson, J, et al. (2021) Photocrosslinked Bioreducible Polymeric Nanoparticles for Enhanced Systemic siRNA Delivery as Cancer Therapy. Advanced Functional Materials. 1970 Jan 1;:2009768. Link: Advanced Functional Materials
PB-CMV-GreenPuro-H1-MCS shRNA Cloning and Expression Vector $612.00

Products

Catalog Number Description Size Price Quantity Add to Cart
PBSI505A-1 PB-CMV-GreenPuro-H1-MCS shRNA cloning and expression vector 10 µg $612.00
- +
Contact Us

Overview

Overview

Easy and consistent shRNA delivery and expression Not just for genes, the PiggyBac system is also an excellent choice for reliably producing shRNA. The PB-CMV-GreenPuro-H1-MCS shRNA Cloning and Expression Vector (Cat.# PBSI505A-1) drives production of your shRNA from the strong H1 promoter. The vector also features GFP and puromycin resistance co-expressed from the strong CMV promoter, with co-expression mediated by the T2A element. PB-CMV-GreenPuro-H1-MCS shRNA Cloning and Expression Vector With the PiggyBac Transposon System, you can:
  • Make transgenic cell lines with a single transfection
  • Integrate multiple PiggyBac Vectors in a single transfection
  • Insert an expression cassette into human, mouse, and rat cells
  • Deliver virtually any-sized DNA insert, from 10 – 100 kb
  • Choose from PiggyBac Vectors that express your gene-of-interest from constitutive or inducible promoters and include a variety of markers
  • Determine the number of integration events with the PiggyBac qPCR Copy Number Kit (# PBC100A-1)
Customer Agreements Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a four-month, limited-use license to evaluate the technology. For end user license information, see the following: * SBI is fully licensed to distribute PiggyBac vectors as a partnership with Hera BioLabs, Inc.

How It Works

How It Works

The PiggyBac Transposon System’s Cut-and-Paste Mechanism

The efficient PiggyBac Transposon System uses a cut-and-paste mechanism to transfer DNA from the PiggyBac Vector into the genome. If only temporary genomic integration is desired, the Excision-only PiggyBac Transposase can be transiently expressed for footprint-free removal of the insert, resulting in reconstitution of the original genome sequence.

The PiggyBac Transposon System’s cut-and-paste mechanism

Figure 1. The PiggyBac Transposon System’s cut-and-paste mechanism.

  • The Super PiggyBac Transposase binds to specific inverted terminal repeats (ITRs) in the PiggyBac Cloning and Expression Vector and excises the ITRs and intervening DNA.
  • The Super PiggyBac Transposase inserts the ITR-Expression Cassette-ITR segment into the genome at TTAA sites.
  • The Excision-only Super PiggyBac Transposase can be used to remove the ITR-Expression Cassette-ITR segment from the genome, for footprint-free removal

Supporting Data

Citations

  • Wang, S, et al. (2021) Budding epithelial morphogenesis driven by cell-matrix versus cell-cell adhesion. Cell. 1970 Jan 1;. PM ID: 34133940
  • Ng, YH, et al. (2021) Efficient generation of dopaminergic induced neuronal cells with midbrain characteristics. Stem cell reports. 1970 Jan 1;. PM ID: 34171286
  • Ukaji, T, et al. (2021) Novel knock-in mouse model for the evaluation of the therapeutic efficacy and toxicity of human podoplanin-targeting agents. Cancer science. 1970 Jan 1; 112(6):2299-2313. PM ID: 33735501
  • Ichikawa, M, et al. (2021) Generation of tetracycline-controllable CYP3A4-expressing Caco-2 cells by the piggyBac transposon system. Scientific reports. 1970 Jan 1; 11(1):11670. PM ID: 34083621
  • Sato, Y & Kimura, H. (2021) Dynamic Behavior of Inactive X Chromosome Territory During the Cell Cycle as Revealed by H3K27me3-Specific Intracellular Antibody. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2329:237-247. PM ID: 34085227
  • Kim, JS, Pineda, M & Li, P. (2021) Reconstitution of Morphogen Signaling Gradients in Cultured Cells. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2258:43-56. PM ID: 33340353
  • Thomas, HF, et al. (2021) Temporal dissection of an enhancer cluster reveals distinct temporal and functional contributions of individual elements. Molecular cell. 1970 Jan 1;. PM ID: 33482114
  • Van, MV, Fujimori, T & Bintu, L. (2021) Nanobody-mediated control of gene expression and epigenetic memory. Nature communications. 1970 Jan 1; 12(1):537. PM ID: 33483487
  • Geis, M, et al. (2021) Combinatorial targeting of multiple myeloma by complementing T cell engaging antibody fragments. Communications biology. 1970 Jan 1; 4(1):44. PM ID: 33420283
  • Saijoh, S, et al. (2021) Discovery of a chemical compound that suppresses expression of BEX2, a dormant cancer stem cell-related protein. Biochemical and biophysical research communications. 1970 Jan 1; 537:132-139. PM ID: 33412384
  • Lam, N, Yamanaka, S & Perli, S. (2021) CRISPRi mediated Down regulation of SFPQ Gene Expression in Human induced Pluripotent Stem Cells Results in Massive Cell Death. Research Square. 1970 Jan 1;. Link: Research Square
  • Xiang, K & Bartel, D. (2021) The molecular basis of coupling between poly(A)-tail length and translational efficiency. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Sugimoto, S, et al. (2021) An organoid-based organ-repurposing approach to treat short bowel syndrome. Nature. 1970 Jan 1;. PM ID: 33627870
  • Ma, P, et al. (2021) Avidity‐Based Selection of Tissue‐Specific CAR‐T Cells from a Combinatorial Cellular Library of CARs. Advanced Science. 1970 Jan 1;:2003091. Link: Advanced Science
  • Cho, JH, et al. (2021) Engineering advanced logic and distributed computing in human CAR immune cells. Nature communications. 1970 Jan 1; 12(1):792. PM ID: 33542232
  • Omori, S, et al. (2021) Tim4 recognizes carbon nanotubes and mediates phagocytosis leading to granuloma formation. Cell reports. 1970 Jan 1; 34(6):108734. PM ID: 33567275
  • Tjalsma, SJD, et al. (2021) H4K20me1 and H3K27me3 are concurrently loaded onto the inactive X chromosome but dispensable for inducing gene silencing. EMBO reports. 1970 Jan 1;:e51989. PM ID: 33605056
  • Kitano, H, et al. (2021) Development of a genetically modified hepatoma cell line with heat-inducible high liver function. Cytotechnology. 1970 Jan 1;. Link: Cytotechnology
  • Zhu, R, et al. (2021) Synthetic multistability in mammalian cells. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Karlsson, J, et al. (2021) Photocrosslinked Bioreducible Polymeric Nanoparticles for Enhanced Systemic siRNA Delivery as Cancer Therapy. Advanced Functional Materials. 1970 Jan 1;:2009768. Link: Advanced Functional Materials