The protocol is quick and easy, requiring only 10 minutes of hands-on time. Simply add ExoQuick-TC ULTRA to 5 mL of tissue culture media or other biofluid, incubate overnight at 4°C, collect EVs by spinning at 3,000g for 10 minutes, resuspend EVs and add to pre-washed ExoQuick-TC ULTRA columns, and spin at 1,000g for 30 seconds to collect the EVs, which are then ready to use.