The pPACKH1 Packaging Plasmid Mix contains three plasmids that produce all the structural and replication proteins needed to transcribe and package an RNA copy of the expression lentivector into recombinant, VSV-G-pseudo typed lentiviral particles. Co-transfect the pPACKH1 Plasmids and your lentivector construct into 293TN cells or other lentiviral production cells, isolate virus particles, and transduce into target cells. Upon transduction, the lentivector construct becomes stably integrated into the genome of the target cells for long-term expression of genes, miRNAs, shRNAs, etc.