pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector

Get another option for robust, coordinated dual gene expression with the pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Lentivector
  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES

Products

Catalog Number Description Size Price Quantity Add to Cart
CD811A-1 pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter, Cloning and Expression Lentivector 10 µg $568
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest and a GFP reporter.

This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.

In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:
  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells
References
  1. Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.

How It Works

Supporting Data

Supporting Data

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors.

Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.

Resources

Citations

  • Liu, YY, et al. (2022) O-GlcNAcylation of MORC2 at threonine 556 by OGT couples TGF-β signaling to breast cancer progression. Cell death and differentiation. 1970 Jan 1;. PM ID: 34974534
  • Lockhart-Cairns, M, et al. (2022) Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biology. 1970 Jan 1;. Link: Matrix Biology
  • Ma, S, et al. (2022) PDGF-D-PDGFRβ signaling enhances IL-15-mediated human natural killer cell survival. Proceedings of the National Academy of Sciences of the United States of America. 1970 Jan 1; 119(3). PM ID: 35027451
  • Kuravi, S, et al. (2022) Functional characterization of NPM1-TYK2 fusion oncogene. NPJ precision oncology. 1970 Jan 1; 6(1):3. PM ID: 35042970
  • Kervarrec, T, et al. (2022) Recurrent FOXK1::GRHL and GPS2::GRHL fusions in trichogerminoma. The Journal of pathology. 1970 Jan 1;. PM ID: 35049062
  • Zhang, X, et al. (2022) T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus. mBio. 1970 Jan 1;:e0286021. PM ID: 35073759
  • Richter, A, et al. (2022) The Molecular Subtype of Adult Acute Lymphoblastic Leukemia Samples Determines the Engraftment Site and Proliferation Kinetics in Patient-Derived Xenograft Models. Cells. 1970 Jan 1; 11(1). PM ID: 35011712
  • Kim, WK, et al. (2022) Heat-Shock Proteins Can Potentiate the Therapeutic Ability of Cryopreserved Mesenchymal Stem Cells for the Treatment of Acute Spinal Cord Injury in Dogs. Stem cell reviews and reports. 1970 Jan 1;. PM ID: 35001344
  • Gao, Y, et al. (2022) Golgi phosphoprotein 3 promotes angiogenesis and sorafenib resistance in hepatocellular carcinoma via upregulating exosomal miR-494-3p. Cancer cell international. 1970 Jan 1; 22(1):35. PM ID: 35073936
  • Khan, AA, et al. (2022) WBSCR22 and TRMT112 synergistically suppress cell proliferation, invasion and tumorigenesis in pancreatic cancer via transcriptional regulation of ISG15. International journal of oncology. 1970 Jan 1; 60(3). PM ID: 35088887
  • Ilina, E, et al. (2022) Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration. iScience. 1970 Jan 1;:103842. Link: iScience
  • Kim, M, et al. (2022) A Recombinant Ig Fragment (IgCw-γεκ) Comprising the Cγ1-Cε2-4 and Cκ Domains Is an Alternative Reagent to Human IgE. Journal of immunology (Baltimore, Md. : 1950). 1970 Jan 1; 208(3):772-779. PM ID: 35022271
  • Martin, M, et al. (2022) The immune-specific E3 ubiquitin ligase MARCH1 is upregulated during HCMV infection to regulate iron levels. Journal of virology. 1970 Jan 1;:jvi0180621. PM ID: 35045264
  • Yang, B, et al. (2022) Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis. Journal of virology. 1970 Jan 1;:JVI0182721. PM ID: 35020472
  • Huang, H, et al. (2022) TSPAN1-elevated FAM110A promotes pancreatic cancer progression by transcriptionally regulating HIST1H2BK. Journal of Cancer. 1970 Jan 1; 13(3):906-917. Link: Journal of Cancer
  • Vannini, I, et al. (2022) Overexpression of ultraconserved region 83- induces lung cancer tumorigenesis. PloS one. 1970 Jan 1; 17(1):e0261464. PM ID: 35015757
  • Lal, S & Raffel, C. (2022) Protocols to Manufacture an Oncolytic Measles Virus-Sensitive Immunocompetent Mouse Model of Medulloblastoma. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2423:165-177. PM ID: 34978698
  • Kono, Y, et al. (2022) Nucleoplasmic Lamin C Rapidly Accumulates at Sites of Nuclear Envelope Rupture with BAF and cGAS. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • 林浩, , et al. (2022) 分泌 anti-PD-L1 及 IFNα 的间充质干细胞联合治疗小鼠黑色素瘤. 中国免疫学杂志. 1970 Jan 1;. Link: 中国免疫学杂志
  • Pan, R, et al. (2022) Augmenting NK cell-based immunotherapy by targeting mitochondrial apoptosis. Cell. 1970 Jan 1; 185(9):1521-1538.e18. PM ID: 35447071
pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector $568.00

Products

Catalog Number Description Size Price Quantity Add to Cart
CD811A-1 pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter, Cloning and Expression Lentivector 10 µg $568
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest and a GFP reporter.

This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.

In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:
  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
pCDH-EF1α-MCS-(PGK-GFP) Bidirectional Promoter Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells
References
  1. Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.

How It Works

Supporting Data

Supporting Data

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors.

Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.

Citations

  • Liu, YY, et al. (2022) O-GlcNAcylation of MORC2 at threonine 556 by OGT couples TGF-β signaling to breast cancer progression. Cell death and differentiation. 1970 Jan 1;. PM ID: 34974534
  • Lockhart-Cairns, M, et al. (2022) Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biology. 1970 Jan 1;. Link: Matrix Biology
  • Ma, S, et al. (2022) PDGF-D-PDGFRβ signaling enhances IL-15-mediated human natural killer cell survival. Proceedings of the National Academy of Sciences of the United States of America. 1970 Jan 1; 119(3). PM ID: 35027451
  • Kuravi, S, et al. (2022) Functional characterization of NPM1-TYK2 fusion oncogene. NPJ precision oncology. 1970 Jan 1; 6(1):3. PM ID: 35042970
  • Kervarrec, T, et al. (2022) Recurrent FOXK1::GRHL and GPS2::GRHL fusions in trichogerminoma. The Journal of pathology. 1970 Jan 1;. PM ID: 35049062
  • Zhang, X, et al. (2022) T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Functional Entry Factor for Tick-Borne Encephalitis Virus. mBio. 1970 Jan 1;:e0286021. PM ID: 35073759
  • Richter, A, et al. (2022) The Molecular Subtype of Adult Acute Lymphoblastic Leukemia Samples Determines the Engraftment Site and Proliferation Kinetics in Patient-Derived Xenograft Models. Cells. 1970 Jan 1; 11(1). PM ID: 35011712
  • Kim, WK, et al. (2022) Heat-Shock Proteins Can Potentiate the Therapeutic Ability of Cryopreserved Mesenchymal Stem Cells for the Treatment of Acute Spinal Cord Injury in Dogs. Stem cell reviews and reports. 1970 Jan 1;. PM ID: 35001344
  • Gao, Y, et al. (2022) Golgi phosphoprotein 3 promotes angiogenesis and sorafenib resistance in hepatocellular carcinoma via upregulating exosomal miR-494-3p. Cancer cell international. 1970 Jan 1; 22(1):35. PM ID: 35073936
  • Khan, AA, et al. (2022) WBSCR22 and TRMT112 synergistically suppress cell proliferation, invasion and tumorigenesis in pancreatic cancer via transcriptional regulation of ISG15. International journal of oncology. 1970 Jan 1; 60(3). PM ID: 35088887
  • Ilina, E, et al. (2022) Enzymatic activity of glycosyltransferase GLT8D1 promotes human glioblastoma cell migration. iScience. 1970 Jan 1;:103842. Link: iScience
  • Kim, M, et al. (2022) A Recombinant Ig Fragment (IgCw-γεκ) Comprising the Cγ1-Cε2-4 and Cκ Domains Is an Alternative Reagent to Human IgE. Journal of immunology (Baltimore, Md. : 1950). 1970 Jan 1; 208(3):772-779. PM ID: 35022271
  • Martin, M, et al. (2022) The immune-specific E3 ubiquitin ligase MARCH1 is upregulated during HCMV infection to regulate iron levels. Journal of virology. 1970 Jan 1;:jvi0180621. PM ID: 35045264
  • Yang, B, et al. (2022) Human Cytomegalovirus Hijacks WD Repeat Domain 11 for Virion Assembly Compartment Formation and Virion Morphogenesis. Journal of virology. 1970 Jan 1;:JVI0182721. PM ID: 35020472
  • Huang, H, et al. (2022) TSPAN1-elevated FAM110A promotes pancreatic cancer progression by transcriptionally regulating HIST1H2BK. Journal of Cancer. 1970 Jan 1; 13(3):906-917. Link: Journal of Cancer
  • Vannini, I, et al. (2022) Overexpression of ultraconserved region 83- induces lung cancer tumorigenesis. PloS one. 1970 Jan 1; 17(1):e0261464. PM ID: 35015757
  • Lal, S & Raffel, C. (2022) Protocols to Manufacture an Oncolytic Measles Virus-Sensitive Immunocompetent Mouse Model of Medulloblastoma. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2423:165-177. PM ID: 34978698
  • Kono, Y, et al. (2022) Nucleoplasmic Lamin C Rapidly Accumulates at Sites of Nuclear Envelope Rupture with BAF and cGAS. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • 林浩, , et al. (2022) 分泌 anti-PD-L1 及 IFNα 的间充质干细胞联合治疗小鼠黑色素瘤. 中国免疫学杂志. 1970 Jan 1;. Link: 中国免疫学杂志
  • Pan, R, et al. (2022) Augmenting NK cell-based immunotherapy by targeting mitochondrial apoptosis. Cell. 1970 Jan 1; 185(9):1521-1538.e18. PM ID: 35447071