pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector

Get another option for robust, coordinated dual gene expression with the pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) Bidirectional Promoter Lentivector
  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES

Products

Catalog Number Description Size Price Quantity Add to Cart
CD823A-1 pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Lentivector 10 µg $556.00
- +
Contact Us

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest with two different markers. Co-express your gene-of-interest and GFP via a T2A element, and divergently express a puromycin resistance marker from the PGK promoter. This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1. In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:
  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells
References
  1. Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.

How It Works

Supporting Data

Supporting Data

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors.

Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.

Resources

Citations

  • Correia, AL, et al. (2021) Hepatic stellate cells suppress NK cell-sustained breast cancer dormancy. Nature. 1970 Jan 1; 594(7864):566-571. PM ID: 34079127
  • Huang, H, et al. (2021) Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance. Nature communications. 1970 Jan 1; 12(1):3720. PM ID: 34140524
  • Teo, SLY, et al. (2021) Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay. Nature communications. 1970 Jan 1; 12(1):3721. PM ID: 34140497
  • Maruyama, M, et al. (2021) The efficacy of lapine preconditioned or genetically modified IL4 over-expressing bone marrow-derived mesenchymal stromal cells in corticosteroid-associated osteonecrosis of the femoral head in rabbits. Biomaterials. 1970 Jan 1; 275:120972. PM ID: 34186237
  • Dahlet, T, et al. (2021) E2F6 initiates stable epigenetic silencing of germline genes during embryonic development. Nature communications. 1970 Jan 1; 12(1):3582. PM ID: 34117224
  • Wang, X, et al. (2021) DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity. Cell reports. 1970 Jan 1; 35(12):109281. PM ID: 34161765
  • Tong, Y, et al. (2021) VHL regulates the sensitivity of clear cell renal cell carcinoma to SIRT4-mediated metabolic stress via HIF-1α/HO-1 pathway. Cell death & disease. 1970 Jan 1; 12(7):621. PM ID: 34135317
  • Nagler, A, et al. (2021) Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics. Cell reports. 1970 Jan 1; 35(13):109305. PM ID: 34166618
  • Wurzer, H, et al. (2021) Intrinsic Resistance of Chronic Lymphocytic Leukemia Cells to NK Cell-Mediated Lysis Can Be Overcome In Vitro by Pharmacological Inhibition of Cdc42-Induced Actin Cytoskeleton Remodeling. Frontiers in immunology. 1970 Jan 1; 12:619069. PM ID: 34108958
  • Yano, K, et al. (2021) PRPF19 regulates p53-dependent cellular senescence by modulating alternative splicing of MDM4 mRNA. The Journal of biological chemistry. 1970 Jan 1;:100882. PM ID: 34144037
  • Mukai, N, et al. (2021) Cardiomyocyte-specific Txnip C247S mutation improves left ventricular functional reserve in streptozotocin-induced diabetic mice. American journal of physiology. Heart and circulatory physiology. 1970 Jan 1;. PM ID: 34085839
  • Wang, CM, et al. (2021) MICA*019 Allele and Soluble MICA as Biomarkers for Ankylosing Spondylitis in Taiwanese. Journal of Personalized Medicine. 1970 Jan 1; 11(6):564. Link: Journal of Personalized Medicine
  • Gheidari, F, et al. (2021) miR-424 induces apoptosis in glioblastoma cells and targets AKT1 and RAF1 oncogenes from the ERBB signaling pathway. European journal of pharmacology. 1970 Jan 1; 906:174273. PM ID: 34153339
  • Nguyen, D, et al. (2021) The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity. Scientific Reports. 1970 Jan 1; 11(1). Link: Scientific Reports
  • Ilina, E, et al. (2021) Enzymatic Activity of Glycosyltransferase Glt8d1 Promotes Human Glioblastoma Cells Migration. SSRN Electronic Journal. 1970 Jan 1;. Link: SSRN Electronic Journal
  • Xylander, A. (2021) Analyse der Effekte der MEK1S212N-Mutation hinsichtlich Signaltransduktion und Phänotyp in Rattenfibroblasten und HEK293-Zellen. Thesis. 1970 Jan 1;. Link: Thesis
  • Wang, J, et al. (2021) Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individualized brain repair of severe ischemic stroke. Bioactive Materials. 1970 Jan 1; 6(7):1988-1999. Link: Bioactive Materials
  • Li, Z, et al. (2021) Acetyl-CoA Synthetase 2: A Critical Linkage in Obesity-Induced Tumorigenesis in Myeloma. Cell metabolism. 1970 Jan 1; 33(1):78-93.e7. PM ID: 33406405
  • Choi, Y, et al. (2021) Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer. Advanced Science. 1970 Jan 1;:2002497. Link: Advanced Science
  • Qing, Y, et al. (2021) R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis. Molecular cell. 1970 Jan 1;. PM ID: 33434505
pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector $556.00

Products

Catalog Number Description Size Price Quantity Add to Cart
CD823A-1 pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) Cloning and Expression Lentivector 10 µg $556.00
- +
Contact Us

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest with two different markers. Co-express your gene-of-interest and GFP via a T2A element, and divergently express a puromycin resistance marker from the PGK promoter. This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1. In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:
  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells
References
  1. Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.

How It Works

Supporting Data

Supporting Data

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors.

Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.

Citations

  • Correia, AL, et al. (2021) Hepatic stellate cells suppress NK cell-sustained breast cancer dormancy. Nature. 1970 Jan 1; 594(7864):566-571. PM ID: 34079127
  • Huang, H, et al. (2021) Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance. Nature communications. 1970 Jan 1; 12(1):3720. PM ID: 34140524
  • Teo, SLY, et al. (2021) Unravelling cytosolic delivery of cell penetrating peptides with a quantitative endosomal escape assay. Nature communications. 1970 Jan 1; 12(1):3721. PM ID: 34140497
  • Maruyama, M, et al. (2021) The efficacy of lapine preconditioned or genetically modified IL4 over-expressing bone marrow-derived mesenchymal stromal cells in corticosteroid-associated osteonecrosis of the femoral head in rabbits. Biomaterials. 1970 Jan 1; 275:120972. PM ID: 34186237
  • Dahlet, T, et al. (2021) E2F6 initiates stable epigenetic silencing of germline genes during embryonic development. Nature communications. 1970 Jan 1; 12(1):3582. PM ID: 34117224
  • Wang, X, et al. (2021) DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity. Cell reports. 1970 Jan 1; 35(12):109281. PM ID: 34161765
  • Tong, Y, et al. (2021) VHL regulates the sensitivity of clear cell renal cell carcinoma to SIRT4-mediated metabolic stress via HIF-1α/HO-1 pathway. Cell death & disease. 1970 Jan 1; 12(7):621. PM ID: 34135317
  • Nagler, A, et al. (2021) Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics. Cell reports. 1970 Jan 1; 35(13):109305. PM ID: 34166618
  • Wurzer, H, et al. (2021) Intrinsic Resistance of Chronic Lymphocytic Leukemia Cells to NK Cell-Mediated Lysis Can Be Overcome In Vitro by Pharmacological Inhibition of Cdc42-Induced Actin Cytoskeleton Remodeling. Frontiers in immunology. 1970 Jan 1; 12:619069. PM ID: 34108958
  • Yano, K, et al. (2021) PRPF19 regulates p53-dependent cellular senescence by modulating alternative splicing of MDM4 mRNA. The Journal of biological chemistry. 1970 Jan 1;:100882. PM ID: 34144037
  • Mukai, N, et al. (2021) Cardiomyocyte-specific Txnip C247S mutation improves left ventricular functional reserve in streptozotocin-induced diabetic mice. American journal of physiology. Heart and circulatory physiology. 1970 Jan 1;. PM ID: 34085839
  • Wang, CM, et al. (2021) MICA*019 Allele and Soluble MICA as Biomarkers for Ankylosing Spondylitis in Taiwanese. Journal of Personalized Medicine. 1970 Jan 1; 11(6):564. Link: Journal of Personalized Medicine
  • Gheidari, F, et al. (2021) miR-424 induces apoptosis in glioblastoma cells and targets AKT1 and RAF1 oncogenes from the ERBB signaling pathway. European journal of pharmacology. 1970 Jan 1; 906:174273. PM ID: 34153339
  • Nguyen, D, et al. (2021) The ATF6β-calreticulin axis promotes neuronal survival under endoplasmic reticulum stress and excitotoxicity. Scientific Reports. 1970 Jan 1; 11(1). Link: Scientific Reports
  • Ilina, E, et al. (2021) Enzymatic Activity of Glycosyltransferase Glt8d1 Promotes Human Glioblastoma Cells Migration. SSRN Electronic Journal. 1970 Jan 1;. Link: SSRN Electronic Journal
  • Xylander, A. (2021) Analyse der Effekte der MEK1S212N-Mutation hinsichtlich Signaltransduktion und Phänotyp in Rattenfibroblasten und HEK293-Zellen. Thesis. 1970 Jan 1;. Link: Thesis
  • Wang, J, et al. (2021) Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individualized brain repair of severe ischemic stroke. Bioactive Materials. 1970 Jan 1; 6(7):1988-1999. Link: Bioactive Materials
  • Li, Z, et al. (2021) Acetyl-CoA Synthetase 2: A Critical Linkage in Obesity-Induced Tumorigenesis in Myeloma. Cell metabolism. 1970 Jan 1; 33(1):78-93.e7. PM ID: 33406405
  • Choi, Y, et al. (2021) Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer. Advanced Science. 1970 Jan 1;:2002497. Link: Advanced Science
  • Qing, Y, et al. (2021) R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis. Molecular cell. 1970 Jan 1;. PM ID: 33434505