pCDH-EF1α-MCS-(PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector

Get another option for robust, coordinated dual gene expression with the pCDH-EF1α-MCS-(PGK-Puro) Bidirectional Promoter Lentivector
  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES

Products

Catalog Number Description Size Price Quantity Add to Cart
CD810A-1 pCDH-EF1α-MCS-(PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector 10 µg $600
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-(PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest and a puromycin resistance marker.

This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.

In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:
  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
pCDH-EF1α-MCS-(PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells
References
  1. Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.

How It Works

Supporting Data

Supporting Data

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors.

Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.

FAQs

Resources

Citations

  • Ge, LP, et al. (2024) ZNF689 deficiency promotes intratumor heterogeneity and immunotherapy resistance in triple-negative breast cancer. Cell research. 2024; 34(1):58-75. PM ID: 38168642
  • Zhai, X, et al. (2024) LDLR is used as a cell entry receptor by multiple alphaviruses. Nature communications. 2024; 15(1):622. PM ID: 38245515
  • Sharma, P, et al. (2024) Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners. Nature communications. 2024; 15(1):473. PM ID: 38212624
  • Zhu, Q, et al. (2024) Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells. Nature communications. 2024; 15(1):40. PM ID: 38167292
  • Chen, SY, et al. (2024) Spermatid perinuclear RNA-binding protein promotes UBR5-mediated proteolysis of Dicer to accelerate triple-negative breast cancer progression. Cancer letters. 2024;:216672. PM ID: 38280476
  • Shi, T, et al. (2024) Bivalent activity of super-enhancer RNA LINC02454 controls 3D chromatin structure and regulates glioma sensitivity to temozolomide. Cell death & disease. 2024; 15(1):6. PM ID: 38177123
  • Rivera, M, et al. (2024) Malignant A-to-I RNA editing by ADAR1 drives T cell acute lymphoblastic leukemia relapse via attenuating dsRNA sensing. Cell reports. 2024; 43(2):113704. PM ID: 38265938
  • He, J, et al. (2024) Exosome-specific loading Sox10 for the treatment of Cuprizone-induced demyelinating model. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2024; 171:116128. PM ID: 38218078
  • Xiao, G, et al. (2024) Fbxw7 suppresses carcinogenesis and stemness in triple-negative breast cancer through CHD4 degradation and Wnt/β-catenin pathway inhibition. Journal of translational medicine. 2024; 22(1):99. PM ID: 38268032
  • Wang, Q, et al. (2024) The pleiotropic enhancer enh9 promotes cell proliferation and migration in non-small cell lung cancer via ERMP1 and PD-L1. Biochimica et biophysica acta. Molecular basis of disease. 2024; 1870(3):167015. PM ID: 38182069
  • Samer, C, et al. (2024) Multi-targeted loss of the antigen presentation molecule MR1 during HSV-1 and HSV-2 infection. iScience. 2024; 27(2):108801. PM ID: 38303725
  • Shen, Z, et al. (2024) Intrahepatic homeobox protein MSX-1 is a novel host restriction factor of hepatitis B virus. Journal of virology. 2024;:e0134523. PM ID: 38226815
  • Wu, H, et al. (2024) METTL14/miR-29c-3p axis drives aerobic glycolysis to promote triple-negative breast cancer progression though TRIM9-mediated PKM2 ubiquitination. Journal of cellular and molecular medicine. 2024;. PM ID: 38263865
  • Gong, Y, et al. (2024) Transcriptome sequencing analysis reveals miR-30c-5p promotes ferroptosis in cervical cancer and inhibits growth and metastasis of cervical cancer xenografts by targeting the METTL3/KRAS axis. Cellular signalling. 2024;:111068. PM ID: 38286198
  • Hong, J, et al. (2024) All-in-one IQ toggle switches with high versatilities for fine tuning of transgene expression in mammalian cells and tissues. Molecular Therapy - Methods & Clinical Development. 2024;:101202. Link: Molecular Therapy - Methods & Clinical Development
  • Li, J, et al. (2024) ECM1-associated miR-1260b promotes osteogenic differentiation by targeting GDI1. Acta histochemica. 2024; 126(1):152133. PM ID: 38266317
  • Yang, W, et al. (2024) Protein SUMOylation promotes cAMP-independent EPAC1 activation. bioRxiv : the preprint server for biology. 2024;. PM ID: 38260470
  • Wu, Z, et al. (2024) Targeting CaMKII-δ/DHCR24 axis is a novel strategy against acute myeloid leukemia. Research Square. 2024;. Link: Research Square
  • Bataclan, M, et al. (2024) Crosstalk between the RNA-binding proteins Regnase-1 and -3 shapes mast cell survival and cytokine expression. bioRxiv. 2024;. Link: bioRxiv
  • Giblin, A, et al. (2024) Neuronal polyunsaturated fatty acids are protective in FTD/ALS. bioRxiv. 2024;. Link: bioRxiv
pCDH-EF1α-MCS-(PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector $600.00

Products

Catalog Number Description Size Price Quantity Add to Cart
CD810A-1 pCDH-EF1α-MCS-(PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector 10 µg $600
- +

Overview

Overview

Get robust, stable expression of your gene-of-interest in dividing and quiescent cells

Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-(PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest and a puromycin resistance marker.

This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.

In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:
  • Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
  • Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
pCDH-EF1α-MCS-(PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
  • Multiple promoter options
  • Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
  • Coordinated co-expression via T2A or IRES
  • Single promoter, dual promoter, and bidirectional promoter formats
  • High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
Not sure which promoter is right for you? This table should help:
PromoterExpression LevelApplication
CMVHighCommonly used in most cell lines (HeLa, HEK293, HT1080, etc.)
MSCVHighHematopoietic and stem cells
EF1αMediumMost cell types including primary cells and stem cells
PGKMediumMost cell types including primary cells and stem cells
UbCLowMost cell types including primary cells and stem cells
References
  1. Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.

How It Works

Supporting Data

Supporting Data

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors

Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors.

Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.

FAQs

Citations

  • Ge, LP, et al. (2024) ZNF689 deficiency promotes intratumor heterogeneity and immunotherapy resistance in triple-negative breast cancer. Cell research. 2024; 34(1):58-75. PM ID: 38168642
  • Zhai, X, et al. (2024) LDLR is used as a cell entry receptor by multiple alphaviruses. Nature communications. 2024; 15(1):622. PM ID: 38245515
  • Sharma, P, et al. (2024) Disordered-to-ordered transitions in assembly factors allow the complex II catalytic subunit to switch binding partners. Nature communications. 2024; 15(1):473. PM ID: 38212624
  • Zhu, Q, et al. (2024) Stabilization of Pin1 by USP34 promotes Ubc9 isomerization and protein sumoylation in glioma stem cells. Nature communications. 2024; 15(1):40. PM ID: 38167292
  • Chen, SY, et al. (2024) Spermatid perinuclear RNA-binding protein promotes UBR5-mediated proteolysis of Dicer to accelerate triple-negative breast cancer progression. Cancer letters. 2024;:216672. PM ID: 38280476
  • Shi, T, et al. (2024) Bivalent activity of super-enhancer RNA LINC02454 controls 3D chromatin structure and regulates glioma sensitivity to temozolomide. Cell death & disease. 2024; 15(1):6. PM ID: 38177123
  • Rivera, M, et al. (2024) Malignant A-to-I RNA editing by ADAR1 drives T cell acute lymphoblastic leukemia relapse via attenuating dsRNA sensing. Cell reports. 2024; 43(2):113704. PM ID: 38265938
  • He, J, et al. (2024) Exosome-specific loading Sox10 for the treatment of Cuprizone-induced demyelinating model. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie. 2024; 171:116128. PM ID: 38218078
  • Xiao, G, et al. (2024) Fbxw7 suppresses carcinogenesis and stemness in triple-negative breast cancer through CHD4 degradation and Wnt/β-catenin pathway inhibition. Journal of translational medicine. 2024; 22(1):99. PM ID: 38268032
  • Wang, Q, et al. (2024) The pleiotropic enhancer enh9 promotes cell proliferation and migration in non-small cell lung cancer via ERMP1 and PD-L1. Biochimica et biophysica acta. Molecular basis of disease. 2024; 1870(3):167015. PM ID: 38182069
  • Samer, C, et al. (2024) Multi-targeted loss of the antigen presentation molecule MR1 during HSV-1 and HSV-2 infection. iScience. 2024; 27(2):108801. PM ID: 38303725
  • Shen, Z, et al. (2024) Intrahepatic homeobox protein MSX-1 is a novel host restriction factor of hepatitis B virus. Journal of virology. 2024;:e0134523. PM ID: 38226815
  • Wu, H, et al. (2024) METTL14/miR-29c-3p axis drives aerobic glycolysis to promote triple-negative breast cancer progression though TRIM9-mediated PKM2 ubiquitination. Journal of cellular and molecular medicine. 2024;. PM ID: 38263865
  • Gong, Y, et al. (2024) Transcriptome sequencing analysis reveals miR-30c-5p promotes ferroptosis in cervical cancer and inhibits growth and metastasis of cervical cancer xenografts by targeting the METTL3/KRAS axis. Cellular signalling. 2024;:111068. PM ID: 38286198
  • Hong, J, et al. (2024) All-in-one IQ toggle switches with high versatilities for fine tuning of transgene expression in mammalian cells and tissues. Molecular Therapy - Methods & Clinical Development. 2024;:101202. Link: Molecular Therapy - Methods & Clinical Development
  • Li, J, et al. (2024) ECM1-associated miR-1260b promotes osteogenic differentiation by targeting GDI1. Acta histochemica. 2024; 126(1):152133. PM ID: 38266317
  • Yang, W, et al. (2024) Protein SUMOylation promotes cAMP-independent EPAC1 activation. bioRxiv : the preprint server for biology. 2024;. PM ID: 38260470
  • Wu, Z, et al. (2024) Targeting CaMKII-δ/DHCR24 axis is a novel strategy against acute myeloid leukemia. Research Square. 2024;. Link: Research Square
  • Bataclan, M, et al. (2024) Crosstalk between the RNA-binding proteins Regnase-1 and -3 shapes mast cell survival and cytokine expression. bioRxiv. 2024;. Link: bioRxiv
  • Giblin, A, et al. (2024) Neuronal polyunsaturated fatty acids are protective in FTD/ALS. bioRxiv. 2024;. Link: bioRxiv