pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector
- Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
- Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CD822A-1 | pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Lentivector | 10 µg | $579 |
|
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Overview
Overview
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest with two different markers. Co-express your gene-of-interest and RFP via a T2A element, and divergently express a puromycin resistance marker from the PGK promoter.
This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.
In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:- Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
- Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
- Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.
How It Works
Supporting Data
Supporting Data
Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors
Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.
FAQs
Resources
Related Products
Citations
-
Sarangi, SK, Lande, KM & Kumar, S. (2023) KIR signaling is regulated by electrostatic interaction of its cytosolic tail with the plasma membrane despite being neutral polyampholyte. Proceedings of the National Academy of Sciences of the United States of America. 2023; 120(1):e2212987120. PM ID: 36574700
-
Fang, Z, et al. (2023) Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation. Signal transduction and targeted therapy. 2023; 8(1):16. PM ID: 36627278
-
Lee, SA, et al. (2023) OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis. Nature cell biology. 2023; 25(1):92-107. PM ID: 36604592
-
Li, C, et al. (2023) Novel CD19-specific γ/δ TCR-T cells in relapsed or refractory diffuse large B-cell lymphoma. Journal of hematology & oncology. 2023; 16(1):5. PM ID: 36681817
-
Zhao, S, et al. (2023) Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infection. Nature communications. 2023; 14(1):478. PM ID: 36717589
-
Crowley, MJP, et al. (2023) Tumor-intrinsic IRE1α signaling controls protective immunity in lung cancer. Nature communications. 2023; 14(1):120. PM ID: 36624093
-
Wang, SQ, et al. (2023) Targeting GSTP1 as Therapeutic Strategy against Lung Adenocarcinoma Stemness and Resistance to Tyrosine Kinase Inhibitors. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2023;:e2205262. PM ID: 36709476
-
Wang, R, et al. (2023) ZBTB18 restricts chromatin accessibility and prevents transcriptional adaptations that drive metastasis. Science advances. 2023; 9(1):eabq3951. PM ID: 36608120
-
Yang, C, et al. (2023) Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules. Autophagy. 2023;:1-18. PM ID: 36692217
-
Sahu, S, et al. (2023) Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP7. Biomolecules. 2023; 13(1). PM ID: 36671538
-
Xi, S, et al. (2023) Cigarette Smoke Enhances the Malignant Phenotype of Esophageal Adenocarcinoma Cells by Disrupting a Repressive Regulatory Interaction Between miR-145 and LOXL2. Laboratory Investigation. 2023; 103(4):100014. Link: Laboratory Investigation
-
Tsubosaka, M, et al. (2023) The efficiency of genetically modified mesenchymal stromal cells combined with a functionally graded scaffold for bone regeneration in corticosteroid-induced osteonecrosis of the femoral head in rabbits. Journal of biomedical materials research. Part A. 2023;. PM ID: 36606330
-
Guo, C, et al. (2023) Engagement of an optimized lentiviral vector enhances the expression and cytotoxicity of CAR in human NK cells. Molecular Immunology. 2023; 155:91-99. Link: Molecular Immunology
-
Zhang, Y, et al. (2023) Development of stable HEK293T cell pools expressing CSFV E2 protein: A potential antigen expression platform. Vaccine. 2023;. PM ID: 36725430
-
Leland, P, et al. (2023) Characterization of Chimeric Antigen Receptor Modified T Cells Expressing scFv-IL-13Rα2 after Radiolabeling with 89Zirconium Oxine for PET Imaging. Research square. 2023;. PM ID: 36711796
-
Yi, L, et al. (2023) Aurora kinase inhibitors regulate T memory stem cell phenotype in T cell receptor-engineered T cells with prolonged persistence. Research Square. 2023;. Link: Research Square
-
ROBINSON, K, et al. (2023) Corynebacterium diphtheriaecauses keratinocyte-intrinsic ribotoxic stress and NLRP1 inflammasome activation in a model of cutaneous diphtheria. bioRxiv. 2023;. Link: bioRxiv
-
An, H, et al. (2023) Identification of the mouse Kupffer cell receptors recognizing pneumococcal capsules by affinity screening. STAR Protocols. 2023; 4(1):102065. Link: STAR Protocols
-
韩, 留. (2023) Construction of Non-Small Cell Lung Cancer (NSCLC) Cell Lines Stably Overexpressing MST4 or dnMST4 Transgenes. Advances in Clinical Medicine. 2023; 13(01):191-200. Link: Advances in Clinical Medicine
-
Stenzel, M. (2023) 12/15-lipoxygenase and its role in anti-tumor immunity. Thesis. 2023;. Link: Thesis
- See More
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CD822A-1 | pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Lentivector | 10 µg | $579 |
|
||||
Overview
Overview
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest with two different markers. Co-express your gene-of-interest and RFP via a T2A element, and divergently express a puromycin resistance marker from the PGK promoter.
This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.
In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:- Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
- Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
- Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.
How It Works
Supporting Data
Supporting Data
Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors
Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.
FAQs
Citations
-
Sarangi, SK, Lande, KM & Kumar, S. (2023) KIR signaling is regulated by electrostatic interaction of its cytosolic tail with the plasma membrane despite being neutral polyampholyte. Proceedings of the National Academy of Sciences of the United States of America. 2023; 120(1):e2212987120. PM ID: 36574700
-
Fang, Z, et al. (2023) Oxidative stress-triggered Wnt signaling perturbation characterizes the tipping point of lung adeno-to-squamous transdifferentiation. Signal transduction and targeted therapy. 2023; 8(1):16. PM ID: 36627278
-
Lee, SA, et al. (2023) OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis. Nature cell biology. 2023; 25(1):92-107. PM ID: 36604592
-
Li, C, et al. (2023) Novel CD19-specific γ/δ TCR-T cells in relapsed or refractory diffuse large B-cell lymphoma. Journal of hematology & oncology. 2023; 16(1):5. PM ID: 36681817
-
Zhao, S, et al. (2023) Salmonella effector SopB reorganizes cytoskeletal vimentin to maintain replication vacuoles for efficient infection. Nature communications. 2023; 14(1):478. PM ID: 36717589
-
Crowley, MJP, et al. (2023) Tumor-intrinsic IRE1α signaling controls protective immunity in lung cancer. Nature communications. 2023; 14(1):120. PM ID: 36624093
-
Wang, SQ, et al. (2023) Targeting GSTP1 as Therapeutic Strategy against Lung Adenocarcinoma Stemness and Resistance to Tyrosine Kinase Inhibitors. Advanced science (Weinheim, Baden-Wurttemberg, Germany). 2023;:e2205262. PM ID: 36709476
-
Wang, R, et al. (2023) ZBTB18 restricts chromatin accessibility and prevents transcriptional adaptations that drive metastasis. Science advances. 2023; 9(1):eabq3951. PM ID: 36608120
-
Yang, C, et al. (2023) Stress granule homeostasis is modulated by TRIM21-mediated ubiquitination of G3BP1 and autophagy-dependent elimination of stress granules. Autophagy. 2023;:1-18. PM ID: 36692217
-
Sahu, S, et al. (2023) Nucleolar Architecture Is Modulated by a Small Molecule, the Inositol Pyrophosphate 5-InsP7. Biomolecules. 2023; 13(1). PM ID: 36671538
-
Xi, S, et al. (2023) Cigarette Smoke Enhances the Malignant Phenotype of Esophageal Adenocarcinoma Cells by Disrupting a Repressive Regulatory Interaction Between miR-145 and LOXL2. Laboratory Investigation. 2023; 103(4):100014. Link: Laboratory Investigation
-
Tsubosaka, M, et al. (2023) The efficiency of genetically modified mesenchymal stromal cells combined with a functionally graded scaffold for bone regeneration in corticosteroid-induced osteonecrosis of the femoral head in rabbits. Journal of biomedical materials research. Part A. 2023;. PM ID: 36606330
-
Guo, C, et al. (2023) Engagement of an optimized lentiviral vector enhances the expression and cytotoxicity of CAR in human NK cells. Molecular Immunology. 2023; 155:91-99. Link: Molecular Immunology
-
Zhang, Y, et al. (2023) Development of stable HEK293T cell pools expressing CSFV E2 protein: A potential antigen expression platform. Vaccine. 2023;. PM ID: 36725430
-
Leland, P, et al. (2023) Characterization of Chimeric Antigen Receptor Modified T Cells Expressing scFv-IL-13Rα2 after Radiolabeling with 89Zirconium Oxine for PET Imaging. Research square. 2023;. PM ID: 36711796
-
Yi, L, et al. (2023) Aurora kinase inhibitors regulate T memory stem cell phenotype in T cell receptor-engineered T cells with prolonged persistence. Research Square. 2023;. Link: Research Square
-
ROBINSON, K, et al. (2023) Corynebacterium diphtheriaecauses keratinocyte-intrinsic ribotoxic stress and NLRP1 inflammasome activation in a model of cutaneous diphtheria. bioRxiv. 2023;. Link: bioRxiv
-
An, H, et al. (2023) Identification of the mouse Kupffer cell receptors recognizing pneumococcal capsules by affinity screening. STAR Protocols. 2023; 4(1):102065. Link: STAR Protocols
-
韩, 留. (2023) Construction of Non-Small Cell Lung Cancer (NSCLC) Cell Lines Stably Overexpressing MST4 or dnMST4 Transgenes. Advances in Clinical Medicine. 2023; 13(01):191-200. Link: Advances in Clinical Medicine
-
Stenzel, M. (2023) 12/15-lipoxygenase and its role in anti-tumor immunity. Thesis. 2023;. Link: Thesis
- See More