pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector
- Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
- Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CD822A-1 | pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Lentivector | 10 µg | $619 |
|
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Overview
Overview
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest with two different markers. Co-express your gene-of-interest and RFP via a T2A element, and divergently express a puromycin resistance marker from the PGK promoter.
This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.
In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:- Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
- Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
- Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.
References
How It Works
Supporting Data
Supporting Data
Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors
Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.
FAQs
Documentation
Citations
Related Products
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CD822A-1 | pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Cloning and Expression Lentivector | 10 µg | $619 |
|
||||
Overview
Overview
Get robust, stable expression of your gene-of-interest in dividing and quiescent cells
Well-regarded in the industry for reliable gene expression, SBI’s lentiviral vectors come in a variety of formats that support a wide range of applications. The pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) Bidirectional Promoter Cloning and Expression Lentivector gives you another option for robust, coordinated expression of your gene-of-interest with two different markers. Co-express your gene-of-interest and RFP via a T2A element, and divergently express a puromycin resistance marker from the PGK promoter.
This promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter, and this divergent configuration is both stable and resistant to silencing in embryonic stem (ES) cells, trophectoderm stem (TS) cells, and extra embryonic endoderm (XEN) stem cells1.
In addition, SBI’s Bidirectional Promoter Cloning and Expression Lentivectors enable:- Coordinate expression of your gene-of-interest with a marker without the use of IRES elements, which can provide weak co-expression of two cDNAs
- Avoidance of unidirectional promoter interference issues that can sometimes arise when using lentivectors
With over thirty HIV-based lentivector options for gene expression, SBI’s family of pCDH Cloning and Expression Lentivectors provide the right lentivector for almost any gene expression project:
- Multiple promoter options
- Multiple marker options—Puro, Neo, Hygro, GFP, or RFP
- Coordinated co-expression via T2A or IRES
- Single promoter, dual promoter, and bidirectional promoter formats
- High expression in most hematopoietic, embryonic, and stem cells via the MSCV CpG-deficient promoter
| Promoter | Expression Level | Application |
|---|---|---|
| CMV | High | Commonly used in most cell lines (HeLa, HEK293, HT1080, etc.) |
| MSCV | High | Hematopoietic and stem cells |
| EF1α | Medium | Most cell types including primary cells and stem cells |
| PGK | Medium | Most cell types including primary cells and stem cells |
| UbC | Low | Most cell types including primary cells and stem cells |
- Golding MC and Mann MR. A bidirectional promoter architecture enhances lentiviral transgenesis in embryonic and extraembryonic stem cells. Gene Ther. 2011 Aug; 18(8):817-26. PMID: 21390068.
References
How It Works
Supporting Data
Supporting Data
Express multiple transgenes simultaneously with SBI’s Bidirectional Promoter Lentivectors
Sample data showing effective transduction with SBI’s Bidirectional Promoter Lentivectors. HEK293 cells were transduced with pCDH-EF1α-MCS-(PGK-Puro) (Cat.# CDH810A-1, upper left panel), pCDH-EF1α-MCS-(PGK-GFP-T2A-Puro) (Cat.# CDH813A-1, upper right panel), pCDH-EF1α-MCS-T2A-RFP (PGK-Puro) (Cat.# CDH822A-1, bottom left panel), pCDH-EF1α-MCS-T2A-GFP (PGK-Puro) (Cat.# CDH823A-1, bottom right panel). After four days, cells were imaged—the high number of cells present after puromycin selection (upper left panel) and cells showing GFP or RFP signal (all other panels) demonstrates the effectiveness of this lentivector family.