It’s this connection between the cell of origin and an exosome’s contents that’s so key to the burgeoning field of exosome research; including the interplay between exosomes and cancer. Scientists are starting to understand that exosomal contents can serve as useful cancer biomarkers or even biomarkers in plenty of other ailments, such as neurodegenerative diseases. As you might expect, these...

So why are extracellular vesicles, including exosomes, such useful biomarkers then? The major reason for this is quite simple. It’s because they’re present in virtually all the biofluids you can think of (Diagram 1.5). Extracellular vesicles can be found in fluids such as: Serum, plasma Urine Seminal fluid CSF Saliva Tears Breast milk This ubiquity makes extracellular vesicle-based diagnostics that...

In other words, we’re on our way to customizable, patient-centered, treatment approaches. For example, we may be able to identify the proteins or microRNA we need to alter in a given patient. Then that same patient’s exosomes can be isolated, modified with the appropriate siRNA or proteins, and then placed back into the patient for treatment (Diagram 1.6). Diagram 1.6...

Perhaps all of this interesting research has got you excited about extracellular vesicle isolation and the potentials thereof. There are a few things you may want to consider though. The first of which revolves around the confusion about, and lack of consensus of, how to distinguish different subsets of EVs from one another. Luckily in 2014, the International Society for...

As you can imagine given the aforementioned discussion, there are numerous challenges with respect to the use of EVs for biomarker discovery. The two main components of EVs are RNAs and proteins; we’ll start with the challenges surrounding the former and then move on to those involving the latter. Exosomal RNA is mainly comprised of species between 20-200 nt; including...

The need for a post-isolation clean-up is thus very clear. And that’s exactly why we came up with ExoQuick ULTRA. EVs isolated with ExoQuick ULTRA display a morphology you’d expect with EVs (Image 3.1). Image 3.1: EVs Isolated with ExoQuick ULTRA Display Typical EV Morphology. Taken from powerpoint slide show. Please provide a citation. With ExoQuick ULTRA you’re also able...

We believe that ExoQuick ULTRA is by far the market’s best way of processing individual samples. But what if you need an EV isolation system that’s fast, easy, and high-throughput to boot? What if you need to quickly collect and compare EVs from multiple sources at once? We’ve developed the industry’s leading solution for that, too. It’s called the SmartSEC...

Q. Do EVs secrete nucleocapsids into the bloodstream? A. Given the currently available literature on the point, we know that while nucleocapsids can be found inside of EVs, they aren’t secreting them. Q. What’s the size of the vesicles that you can recover with ExoQuick ULTRA? A. Depending on the prep, between 70-200 nm. Q. What’s the best way to...