SeraMir Exosome RNA Amplification Kit for Media & Urine

Everything you need to accurately and sensitively measure exosomal RNAs from media, urine, and other biofluids (except serum, plasma, and ascites fluid).
  • Reliable, reproducible exoRNA isolation from most biofluids
  • Sensitive detection via exoRNA amplification
  • Excellent preparation method for qPCR
  • Can be used to amplify sense-strand exoRNAs for microarray construction

Products

Catalog Number Description Size Price Quantity Add to Cart
RA800TC-1 Complete SeraMir Exosome RNA Amplification kit from Media and Urine (10 mL ExoQuick-TC, 10 exoRNA columns, cDNA synthesis and amplification components) 10 Reactions $617
- +

Overview

Overview

Profile exosomal RNA biomarkers and more

Looking for biomarkers? SBI’s SeraMir Exosome RNA Amplification Kit for Media & Urine can help. Providing everything you need to accurately and sensitively measure exosomal RNAs (exoRNAs) from most biofluids, our SeraMir Exosome RNA Amplification Kit for Media & Urine can take you from sample to amplified exoRNAs in a single day (for exoRNA isolation from serum, plasma, or ascites fluid, use the SeraMir Exosome RNA Amplification Kit for Media and Urine).

  • Reliable, reproducible exoRNA isolation from most biofluids
  • Sensitive detection via exoRNA amplification
  • Excellent preparation method for qPCR
  • Can be used to amplify sense-strand exoRNAs for microarray construction
Find exosomal RNA biomarkers using the SeraMir Exosome RNA Amplification Kit

With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.

The SeraMir Exosome RNA Amplification Kit for Media & Urine includes everything you need to accurately and sensitively measure RNAs from most biofluids—ExoQuick-TC for fast and efficient exosome isolation, a phenol-free lysis buffer and rapid spin columns for exoRNA isolation, and reagents for 3’ tailing and simultaneous tagging of both 5’ and 3’ ends during cDNA synthesis so you can go straight to qPCR.

In addition, primers for PCR amplification are included to make double-stranded cDNA via T7 IVT. These amplified exoRNAs can be used to construct microarrays or for next generation sequencing applications.

Choose the SeraMir Kit that’s right for you
Cat. #NameKit includes
ExoQuick or ExoQuick-TCSeraMir RNA columns and reagentsSeraMir cDNA synthesis and amplification reagents384-well plate miRNAs for human, mouse, or rat
RA800A-1Complete SeraMir Exosome RNA Amplification Kit 
RA800TC-1Complete SeraMir Exosome RNA Amplification Kit for Media and Urine 
RA806A-1SeraMir Exosome RNA Purification Kit  
RA806TC-1SeraMir Exosome RNA Purification Kit for Media and Urine  
RA808A-1SeraMir Exosome RNA Purification Column Kit   
RA820A-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA820TC-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA821A-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA821TC-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA822A-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA822TC-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine

References

How It Works

How It Works

Go from sample to amplified exoRNAs in a single day

  • Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
  • Purify exoRNAs with SeraMir columns
  • Tail and tag all exoRNAs for qPCR
  • Perform second strand synthesis to generate cDNAs for amplification and T7 IVT

The amplified exoRNAs are ready for use in microarrays or NGS. Because RNA ligase is not used, you can avoid complicating adaptor concatemer artifacts.

Isolate serum exosomes and purify exoRNAs

Streamline biomarker discovery with seramir – the workflow, steps 1 and 2

Tail exoRNAs and synthesize double-tagged cDNAStreamline biomarker discovery with seramir – the workflow, steps 3 and 4

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis

Supporting Data

Supporting Data

Better qPCR profiling with SeraMir

SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.

 SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set.
Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.

FAQs

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
RA800TC-1 Complete SeraMir Exosome RNA Amplification kit from Media and Urine (10 mL ExoQuick-TC, 10 exoRNA columns, cDNA synthesis and amplification components) 10 Reactions $617
- +

Overview

Overview

Profile exosomal RNA biomarkers and more

Looking for biomarkers? SBI’s SeraMir Exosome RNA Amplification Kit for Media & Urine can help. Providing everything you need to accurately and sensitively measure exosomal RNAs (exoRNAs) from most biofluids, our SeraMir Exosome RNA Amplification Kit for Media & Urine can take you from sample to amplified exoRNAs in a single day (for exoRNA isolation from serum, plasma, or ascites fluid, use the SeraMir Exosome RNA Amplification Kit for Media and Urine).

  • Reliable, reproducible exoRNA isolation from most biofluids
  • Sensitive detection via exoRNA amplification
  • Excellent preparation method for qPCR
  • Can be used to amplify sense-strand exoRNAs for microarray construction
Find exosomal RNA biomarkers using the SeraMir Exosome RNA Amplification Kit

With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.

The SeraMir Exosome RNA Amplification Kit for Media & Urine includes everything you need to accurately and sensitively measure RNAs from most biofluids—ExoQuick-TC for fast and efficient exosome isolation, a phenol-free lysis buffer and rapid spin columns for exoRNA isolation, and reagents for 3’ tailing and simultaneous tagging of both 5’ and 3’ ends during cDNA synthesis so you can go straight to qPCR.

In addition, primers for PCR amplification are included to make double-stranded cDNA via T7 IVT. These amplified exoRNAs can be used to construct microarrays or for next generation sequencing applications.

Choose the SeraMir Kit that’s right for you
Cat. #NameKit includes
ExoQuick or ExoQuick-TCSeraMir RNA columns and reagentsSeraMir cDNA synthesis and amplification reagents384-well plate miRNAs for human, mouse, or rat
RA800A-1Complete SeraMir Exosome RNA Amplification Kit 
RA800TC-1Complete SeraMir Exosome RNA Amplification Kit for Media and Urine 
RA806A-1SeraMir Exosome RNA Purification Kit  
RA806TC-1SeraMir Exosome RNA Purification Kit for Media and Urine  
RA808A-1SeraMir Exosome RNA Purification Column Kit   
RA820A-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA820TC-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA821A-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA821TC-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA822A-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA822TC-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine

References

How It Works

How It Works

Go from sample to amplified exoRNAs in a single day

  • Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
  • Purify exoRNAs with SeraMir columns
  • Tail and tag all exoRNAs for qPCR
  • Perform second strand synthesis to generate cDNAs for amplification and T7 IVT

The amplified exoRNAs are ready for use in microarrays or NGS. Because RNA ligase is not used, you can avoid complicating adaptor concatemer artifacts.

Isolate serum exosomes and purify exoRNAs

Streamline biomarker discovery with seramir – the workflow, steps 1 and 2

Tail exoRNAs and synthesize double-tagged cDNAStreamline biomarker discovery with seramir – the workflow, steps 3 and 4

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis

Supporting Data

Supporting Data

Better qPCR profiling with SeraMir

SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.

 SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set.
Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.

FAQs

Citations