SeraMir Spike-in RNA Control and qPCR Assay
A great addition to your SeraMir qPCR profiling studies, the SeraMir Spike-in RNA Control and qPCR Assay controls for exoRNA recovery, tailing, and cDNA synthesis.
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| RA805A-1 | SeraMir Control spike-in Small RNA and qPCR assay | 100 Assays | $181 |
|
||||
Overview
Overview
Get extra assurance for your SeraMir qPCR profiling studies
To help researchers with their SeraMir qPCR profiling studies, SBI offers the SeraMir Spike-in RNA Control and qPCR Assay. This product gives researchers the ability to control for exoRNA recovery, tailing, and cDNA synthesis by spiking in known quantities of a control RNA during exoRNA isolation—can be used directly on the 384-well SeraMir profiling plates or in an individual control spike-in qPCR assay.
Choose the SeraMir Kit that’s right for you
| Cat. # | Name | Kit includes | |||
|---|---|---|---|---|---|
| ExoQuick or ExoQuick-TC | SeraMir RNA columns and reagents | SeraMir cDNA synthesis and amplification reagents | 384-well plate miRNAs for human, mouse, or rat | ||
| RA800A-1 | Complete SeraMir Exosome RNA Amplification Kit | ||||
| RA800TC-1 | Complete SeraMir Exosome RNA Amplification Kit for Media and Urine | ||||
| RA806A-1 | SeraMir Exosome RNA Purification Kit | ||||
| RA806TC-1 | SeraMir Exosome RNA Purification Kit for Media and Urine | ||||
| RA808A-1 | SeraMir Exosome RNA Purification Column Kit | ||||
| RA820A-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
| RA820TC-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
| RA821A-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
| RA821TC-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
| RA822A-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
| RA822TC-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
How It Works
How It Works
Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.
If 5 µL of SeraMir Spike-in RNA was used during exoRNA isolation, then you should expect to observe a Ct of about 15 to 20.
Supporting Data
FAQs
Resources
Related Products
Citations
-
Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. 2017;. Link: Conference
-
Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. 2017;. Link: Abstract
-
Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017;. PM ID: 28411393
-
Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017; 25(1):102-110. PM ID: 27883272
-
Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017; 31(9):1277-1295. PM ID: 28447547
-
Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017; 15:163-172. PM ID: 27974246
-
Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017;. PM ID: 28623135
-
Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017;. PM ID: 28580875
-
Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017;:95-99. PM ID: 28643924
-
Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017; 4(2):112-121. PM ID: 28451447
-
Protocol, IIEI. (2017) List of Components. Product. 2017;. Link: Product
-
Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. 2017; 7(4). Link: bio-protocol
-
Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017; 21(1):55-66. PM ID: 28271982
-
Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017; 8(26):42712-42727. PM ID: 28514744
-
Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017;. PM ID: 28157838
-
Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016; 429:39-49. PM ID: 26723490
-
Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. 2016; 5(1):1–94. Link: Liver Cancer
-
Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016; 5:30924. PM ID: 26928673
-
Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016; 11(3):e0150154. PM ID: 26930277
-
Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016; 39:427-432. Link: 中华检验医学杂志
- See More
SeraMir Spike-in RNA Control and qPCR Assay
$181.00
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| RA805A-1 | SeraMir Control spike-in Small RNA and qPCR assay | 100 Assays | $181 |
|
||||
Overview
Overview
Get extra assurance for your SeraMir qPCR profiling studies
To help researchers with their SeraMir qPCR profiling studies, SBI offers the SeraMir Spike-in RNA Control and qPCR Assay. This product gives researchers the ability to control for exoRNA recovery, tailing, and cDNA synthesis by spiking in known quantities of a control RNA during exoRNA isolation—can be used directly on the 384-well SeraMir profiling plates or in an individual control spike-in qPCR assay.
Choose the SeraMir Kit that’s right for you
| Cat. # | Name | Kit includes | |||
|---|---|---|---|---|---|
| ExoQuick or ExoQuick-TC | SeraMir RNA columns and reagents | SeraMir cDNA synthesis and amplification reagents | 384-well plate miRNAs for human, mouse, or rat | ||
| RA800A-1 | Complete SeraMir Exosome RNA Amplification Kit | ||||
| RA800TC-1 | Complete SeraMir Exosome RNA Amplification Kit for Media and Urine | ||||
| RA806A-1 | SeraMir Exosome RNA Purification Kit | ||||
| RA806TC-1 | SeraMir Exosome RNA Purification Kit for Media and Urine | ||||
| RA808A-1 | SeraMir Exosome RNA Purification Column Kit | ||||
| RA820A-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
| RA820TC-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
| RA821A-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
| RA821TC-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
| RA822A-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
| RA822TC-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
How It Works
How It Works
Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.
If 5 µL of SeraMir Spike-in RNA was used during exoRNA isolation, then you should expect to observe a Ct of about 15 to 20.
Supporting Data
FAQs
Citations
-
Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. 2017;. Link: Conference
-
Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. 2017;. Link: Abstract
-
Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017;. PM ID: 28411393
-
Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017; 25(1):102-110. PM ID: 27883272
-
Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017; 31(9):1277-1295. PM ID: 28447547
-
Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017; 15:163-172. PM ID: 27974246
-
Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017;. PM ID: 28623135
-
Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017;. PM ID: 28580875
-
Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017;:95-99. PM ID: 28643924
-
Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017; 4(2):112-121. PM ID: 28451447
-
Protocol, IIEI. (2017) List of Components. Product. 2017;. Link: Product
-
Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. 2017; 7(4). Link: bio-protocol
-
Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017; 21(1):55-66. PM ID: 28271982
-
Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017; 8(26):42712-42727. PM ID: 28514744
-
Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017;. PM ID: 28157838
-
Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016; 429:39-49. PM ID: 26723490
-
Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. 2016; 5(1):1–94. Link: Liver Cancer
-
Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016; 5:30924. PM ID: 26928673
-
Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016; 11(3):e0150154. PM ID: 26930277
-
Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016; 39:427-432. Link: 中华检验医学杂志
- See More