SeraMir Exosome RNA Purification Kit for Media & Urine
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
RA806TC-1 | SeraMir Exosome RNA Purification kit for Media and Urine (10 mL ExoQuick-TC and 10 exoRNA columns) | 10 Preps | $493 |
|
Overview
Overview
Purify exosomal RNAs for biomarker discovery and more—for most biofluids Looking for biomarkers? Studying exosomal RNAs (exoRNAs)? Turn to SBI’s SeraMir Exosome RNA Purification Kit for Media & Urine for optimized isolation of exoRNAs. The kit includes ExoQuick-TC for efficient exosome isolation from most biofulids, and SeraMir columns and reagents for purification of RNA from your isolated exosomes (for exoRNA isolation from serum, plasma, or ascites fluid, try the SeraMir Exosome RNA Purification Kit). Reliable, reproducible exoRNA isolation from most biofluids
With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.
The SeraMir Exosome RNA Purification Kit for Media & Urine includes everything you need for optimized isolation of exosomal RNAs from most biofluids—ExoQuick for fast and efficient exosome isolation and a phenol-free lysis buffer and rapid spin columns to extract RNA from your isolated exosomes.
Choose the SeraMir Kit that’s right for youCat. # | Name | Kit includes | |||
---|---|---|---|---|---|
ExoQuick or ExoQuick-TC | SeraMir RNA columns and reagents | SeraMir cDNA synthesis and amplification reagents | 384-well plate miRNAs for human, mouse, or rat | ||
RA800A-1 | Complete SeraMir Exosome RNA Amplification Kit | ||||
RA800TC-1 | Complete SeraMir Exosome RNA Amplification Kit for Media and Urine | ||||
RA806A-1 | SeraMir Exosome RNA Purification Kit | ||||
RA806TC-1 | SeraMir Exosome RNA Purification Kit for Media and Urine | ||||
RA808A-1 | SeraMir Exosome RNA Purification Column Kit | ||||
RA820A-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA820TC-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
RA821A-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA821TC-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
RA822A-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA822TC-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine |
How It Works
How It Works
Go from sample to amplified exoRNAs in a single day
- Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
- Purify exoRNAs with SeraMir columns (also included)
Purified exoRNAs are fully compatible with most downstream applications, such as qPCR, microarray analysis, or NGS.
Isolate serum exosomes and purify exoRNAs
Tail exoRNAs and synthesize double-tagged cDNA
Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.
Supporting Data
Supporting Data
Better qPCR profiling with SeraMir
Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.
Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set.
Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.
FAQs
Resources
Related Products
Citations
-
Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. 2017;. Link: Conference
-
Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. 2017;. Link: Abstract
-
Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017;. PM ID: 28411393
-
Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017; 25(1):102-110. PM ID: 27883272
-
Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017; 31(9):1277-1295. PM ID: 28447547
-
Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017; 15:163-172. PM ID: 27974246
-
Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017;. PM ID: 28623135
-
Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017;. PM ID: 28580875
-
Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017;:95-99. PM ID: 28643924
-
Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017; 4(2):112-121. PM ID: 28451447
-
Protocol, IIEI. (2017) List of Components. Product. 2017;. Link: Product
-
Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. 2017; 7(4). Link: bio-protocol
-
Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017; 21(1):55-66. PM ID: 28271982
-
Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017; 8(26):42712-42727. PM ID: 28514744
-
Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017;. PM ID: 28157838
-
Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016; 429:39-49. PM ID: 26723490
-
Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. 2016; 5(1):1–94. Link: Liver Cancer
-
Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016; 5:30924. PM ID: 26928673
-
Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016; 11(3):e0150154. PM ID: 26930277
-
Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016; 39:427-432. Link: 中华检验医学杂志
- See More
Products
Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
---|---|---|---|---|---|---|---|---|
RA806TC-1 | SeraMir Exosome RNA Purification kit for Media and Urine (10 mL ExoQuick-TC and 10 exoRNA columns) | 10 Preps | $493 |
|
Overview
Overview
Purify exosomal RNAs for biomarker discovery and more—for most biofluids Looking for biomarkers? Studying exosomal RNAs (exoRNAs)? Turn to SBI’s SeraMir Exosome RNA Purification Kit for Media & Urine for optimized isolation of exoRNAs. The kit includes ExoQuick-TC for efficient exosome isolation from most biofulids, and SeraMir columns and reagents for purification of RNA from your isolated exosomes (for exoRNA isolation from serum, plasma, or ascites fluid, try the SeraMir Exosome RNA Purification Kit). Reliable, reproducible exoRNA isolation from most biofluids
With cargo that reflects the makeup of their parent cells and their easy isolation, researchers are increasingly turning to exosomes as a source of disease-related biomarkers. To simplify and standardize the isolation of RNA from exosomes, SBI has developed the SeraMir family of products.
The SeraMir Exosome RNA Purification Kit for Media & Urine includes everything you need for optimized isolation of exosomal RNAs from most biofluids—ExoQuick for fast and efficient exosome isolation and a phenol-free lysis buffer and rapid spin columns to extract RNA from your isolated exosomes.
Choose the SeraMir Kit that’s right for youCat. # | Name | Kit includes | |||
---|---|---|---|---|---|
ExoQuick or ExoQuick-TC | SeraMir RNA columns and reagents | SeraMir cDNA synthesis and amplification reagents | 384-well plate miRNAs for human, mouse, or rat | ||
RA800A-1 | Complete SeraMir Exosome RNA Amplification Kit | ||||
RA800TC-1 | Complete SeraMir Exosome RNA Amplification Kit for Media and Urine | ||||
RA806A-1 | SeraMir Exosome RNA Purification Kit | ||||
RA806TC-1 | SeraMir Exosome RNA Purification Kit for Media and Urine | ||||
RA808A-1 | SeraMir Exosome RNA Purification Column Kit | ||||
RA820A-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA820TC-1 | Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
RA821A-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA821TC-1 | Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine | ||||
RA822A-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit | ||||
RA822TC-1 | Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine |
How It Works
How It Works
Go from sample to amplified exoRNAs in a single day
- Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
- Purify exoRNAs with SeraMir columns (also included)
Purified exoRNAs are fully compatible with most downstream applications, such as qPCR, microarray analysis, or NGS.
Isolate serum exosomes and purify exoRNAs
Tail exoRNAs and synthesize double-tagged cDNA
Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.
Supporting Data
Supporting Data
Better qPCR profiling with SeraMir
Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 Human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.
Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set.
Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.
FAQs
Citations
-
Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. 2017;. Link: Conference
-
Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. 2017;. Link: Abstract
-
Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017;. PM ID: 28411393
-
Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017; 25(1):102-110. PM ID: 27883272
-
Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017; 31(9):1277-1295. PM ID: 28447547
-
Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017; 15:163-172. PM ID: 27974246
-
Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017;. PM ID: 28623135
-
Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017;. PM ID: 28580875
-
Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017;:95-99. PM ID: 28643924
-
Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017; 4(2):112-121. PM ID: 28451447
-
Protocol, IIEI. (2017) List of Components. Product. 2017;. Link: Product
-
Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. 2017; 7(4). Link: bio-protocol
-
Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017; 21(1):55-66. PM ID: 28271982
-
Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017; 8(26):42712-42727. PM ID: 28514744
-
Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017;. PM ID: 28157838
-
Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016; 429:39-49. PM ID: 26723490
-
Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. 2016; 5(1):1–94. Link: Liver Cancer
-
Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016; 5:30924. PM ID: 26928673
-
Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016; 11(3):e0150154. PM ID: 26930277
-
Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016; 39:427-432. Link: 中华检验医学杂志
- See More