NRP1 Expression Lentivector
- Leverage SBI's robust lentivector expression technology to study NRP1
- Choose ready-to-package plasmid or ready-to-transduce virus
- Also available as a stable cell line that expresses both NRP1 and ACE2 for SARS-CoV-2 studies
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CVD19-140PA-1 | NRP1 Expression Lentivector, pCDH-CMV-NRP1-EF1α-Neo Plasmid | 10 µg | $755 |
|
||||
| CVD19-140VA-1 | NRP1 Expression Lentivector, pCDH-CMV-NRP1-EF1α-Neo Pre-packaged Virus | >2 x 10^6 IFUs | $755 |
|
||||
Overview
Overview
Advance your COVID-19 studies with SBI
SBI is helping scientists around the globe respond to the ongoing COVID-19 pandemic with research tools that get your studies up and running quickly and efficiently. Our NRP1 Expression Lentivector is based on our robust pCDH-CMV-MCS-EF1α-Neo Cloning and Expression Lentivector (Cat.# CD514B-1), and delivers NRP1 expression from the CMV promoter, with a separate EF1α promoter driving the expression of the neomycin resistance gene for selection.
NRP1 is a host factor for SARS-CoV-2 infection that enhances ACE2-mediated cell entry [1, 2], and the NRP1 Expression Lentivector gives you the ability to express NRP1 in the cell line of your choice. Safely study SARS-CoV-2 infection, discover inhibitors, and more.
The NRP1 Expression Lentivector is available in both plasmid and ready-to-transduce pre-packaged lentivirus formats.
- Leverage SBI's robust lentivector expression technology to study NRP1
- Choose ready-to-package plasmid or ready-to-transduce virus
- Also available as a stable cell line that expresses both NRP1 and ACE2 for SARS-CoV-2 studies
See all of our COVID-19-related Expression Constructs
| Catalog Number | Product name |
|---|---|
| CVD19-100PA/VA-1 | ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Plasmid or Pre-packaged Lentivirus) |
| CVD19-140PA/VA-1 | NRP1 Expression Lentivector, pCDH-CMV-NRP1-EF1α-Neo (Plasmid or Pre-packaged Lentivirus) |
| CVD19-120PA/VA-1 | SARS-CoV-2 Nucleocapsid (N protein) Expression Lentivector, pCDH-CMV-SARS-CoV-2-N-EF1α-Puro (Plasmid or Pre-packaged Lentivirus) |
| CVD19-130PA/VA-1 | SARS-CoV-2 Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-S-EF1α-Puro (Plasmid or Pre-packaged Lentivirus) |
| CVD19-135PA/VA-1 | SARS-CoV-2 Humanized Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-hS-EF1α-Puro (Plasmid or Pre-packaged Lentivirus) |
| CVD19-200A-1 | HEK293 ACE2 Stable Cell Line |
| CVD19-260A-1 | HEK293-ACE2-NRP1 Overexpression Stable Cell Line |
| CVD19-220A-1 | HEK293 "N" SARS-CoV-2 Nucleocapsid Overexpression Stable Cell Line |
| CVD19-420A-1 CVD19-425A-1 | "N" SARS-CoV-2 Nucleocapsid mRNA |
| CVD19-430A-1 CVD19-435A-1 | "S" SARS-CoV-2 Spike Glycoprotein mRNA |
| CVD19-440A-1 CVD19-445A-1 | "S" SARS-CoV-2 Spike Omicron mRNA |
- Cantuti-Castelvetri L, et al. Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity. Science. 2020 Nov 13;370(6518):856-860. PMCID: PMC7857391.
- Daly JL, et al. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science. 2020 Nov 13;370(6518):861-865. PMID: 33082294.
How It Works
Supporting Data
Supporting Data
Figure 1. pCDH-CMV-NRP1-EF1α-Neo delivers robust NRP1 expression in HEK293 cells. We transduced the NRP1 Expression Lentivector (pCDH-CMV-NRP1-EF1α-Neo) into HEK293 cells, extracted total protein, and analyzed NRP1 expression via western blot. The parental HEK293 cells (negative control lane) display no NRP1 expression whereas HEK293 cells transduced with the NRP1 Expression Lentivector demonstrate robust NRP1 expression. The positive control of HEK293 cells transfected with the NRP1 Expression Lentivector also displays robust expression.
FAQs
Resources
Related Products
Citations
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| CVD19-140PA-1 | NRP1 Expression Lentivector, pCDH-CMV-NRP1-EF1α-Neo Plasmid | 10 µg | $755 |
|
||||
| CVD19-140VA-1 | NRP1 Expression Lentivector, pCDH-CMV-NRP1-EF1α-Neo Pre-packaged Virus | >2 x 10^6 IFUs | $755 |
|
||||
Overview
Overview
Advance your COVID-19 studies with SBI
SBI is helping scientists around the globe respond to the ongoing COVID-19 pandemic with research tools that get your studies up and running quickly and efficiently. Our NRP1 Expression Lentivector is based on our robust pCDH-CMV-MCS-EF1α-Neo Cloning and Expression Lentivector (Cat.# CD514B-1), and delivers NRP1 expression from the CMV promoter, with a separate EF1α promoter driving the expression of the neomycin resistance gene for selection.
NRP1 is a host factor for SARS-CoV-2 infection that enhances ACE2-mediated cell entry [1, 2], and the NRP1 Expression Lentivector gives you the ability to express NRP1 in the cell line of your choice. Safely study SARS-CoV-2 infection, discover inhibitors, and more.
The NRP1 Expression Lentivector is available in both plasmid and ready-to-transduce pre-packaged lentivirus formats.
- Leverage SBI's robust lentivector expression technology to study NRP1
- Choose ready-to-package plasmid or ready-to-transduce virus
- Also available as a stable cell line that expresses both NRP1 and ACE2 for SARS-CoV-2 studies
See all of our COVID-19-related Expression Constructs
| Catalog Number | Product name |
|---|---|
| CVD19-100PA/VA-1 | ACE2 Expression Lentivector, pCDH-CMV-ACE2-EF1α-Puro (Plasmid or Pre-packaged Lentivirus) |
| CVD19-140PA/VA-1 | NRP1 Expression Lentivector, pCDH-CMV-NRP1-EF1α-Neo (Plasmid or Pre-packaged Lentivirus) |
| CVD19-120PA/VA-1 | SARS-CoV-2 Nucleocapsid (N protein) Expression Lentivector, pCDH-CMV-SARS-CoV-2-N-EF1α-Puro (Plasmid or Pre-packaged Lentivirus) |
| CVD19-130PA/VA-1 | SARS-CoV-2 Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-S-EF1α-Puro (Plasmid or Pre-packaged Lentivirus) |
| CVD19-135PA/VA-1 | SARS-CoV-2 Humanized Spike Glycoprotein (S) Expression Lentivector, pCDH-CMV-SARS-CoV-2-hS-EF1α-Puro (Plasmid or Pre-packaged Lentivirus) |
| CVD19-200A-1 | HEK293 ACE2 Stable Cell Line |
| CVD19-260A-1 | HEK293-ACE2-NRP1 Overexpression Stable Cell Line |
| CVD19-220A-1 | HEK293 "N" SARS-CoV-2 Nucleocapsid Overexpression Stable Cell Line |
| CVD19-420A-1 CVD19-425A-1 | "N" SARS-CoV-2 Nucleocapsid mRNA |
| CVD19-430A-1 CVD19-435A-1 | "S" SARS-CoV-2 Spike Glycoprotein mRNA |
| CVD19-440A-1 CVD19-445A-1 | "S" SARS-CoV-2 Spike Omicron mRNA |
- Cantuti-Castelvetri L, et al. Neuropilin-1 facilitates SARS-CoV-2 cell entry and infectivity. Science. 2020 Nov 13;370(6518):856-860. PMCID: PMC7857391.
- Daly JL, et al. Neuropilin-1 is a host factor for SARS-CoV-2 infection. Science. 2020 Nov 13;370(6518):861-865. PMID: 33082294.
How It Works
Supporting Data
Supporting Data
Figure 1. pCDH-CMV-NRP1-EF1α-Neo delivers robust NRP1 expression in HEK293 cells. We transduced the NRP1 Expression Lentivector (pCDH-CMV-NRP1-EF1α-Neo) into HEK293 cells, extracted total protein, and analyzed NRP1 expression via western blot. The parental HEK293 cells (negative control lane) display no NRP1 expression whereas HEK293 cells transduced with the NRP1 Expression Lentivector demonstrate robust NRP1 expression. The positive control of HEK293 cells transfected with the NRP1 Expression Lentivector also displays robust expression.