NF-κB/Jurkat/GFP™ Transcriptional Reporter Cell Line

Monitor NF-κB signaling in vitro in a physiologically-relevant cell line to screen for genetic and/or small molecule inhibitors and activators, and more
  • Study NF-κB signaling in physiologically-relevant Jurkat cells
  • Use for fluorescence microscopy or FACS
  • Take advantage of the cell line’s 30-fold over background NF-κB-dependent GFP expression
  • Compatible with a variety of screening methods including small molecule and RNAi

Products

Catalog Number Description Size Price Quantity Add to Cart
TR850A-1 NF-kB/Jurkat/GFP Transcriptional Reporter Cell Line 2 x 10^6 Cells $3709
- +

Overview

Overview

Monitor NF-κB signaling in Jurkat cells

Speed your studies with this pre-built Jurkat cell line for monitoring NF-κB signaling in real time. We’ve already integrated an expression cassette that includes NF-κB-responsive transcriptional elements upstream of a minimal CMV promoter (mCMV)-GFP cassette. Expression of GFP (up to 30-fold over background) only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway.

  • Study NF-κB signaling in physiologically-relevant Jurkat cells
  • Use for fluorescence microscopy or FACS
  • Take advantage of the cell line’s 30-fold over background NF-κB-dependent GFP expression
  • Compatible with a variety of screening methods including small molecule and RNAi

How It Works

Supporting Data

Supporting Data

See the NF-κB/Jurkat/GFP Transcriptional Reporter Cell Line in action

Strong GFP fluorescence in response to signaling enables FACS

Strong GFP fluorescence in response to signaling enables FACS

The NF-κB/Jurkat/GFP provides a strong, dose-dependent response to TNF-αThe NF-κB/Jurkat/GFP provides a strong, dose-dependent response to TNF-α

METHODS: NF-B/Jurkat/GFP™ Reporter cells (5×105 cells) were plated at a concentration of 1 million cells/mL into each well of a 24-well plate. TNF-α was added in the amount indicated in the figure. After 24 hours, GFP fluorescence was measured and intensities plotted against TNF-α concentration (A). The fluorescent cells in the original 24-well plate were also photographed on a Zeiss inverted epi-fluorescence microscope (B). Alternatively, 200 μL of cells were fixed with formaldehyde and GFP reporter induction analyzed by flow cytometry, and either the GFP intensities (C) or the percentage of GFP positive cells (D) were plotted against the amount of TNF-α.

The NF-κB/Jurkat/GFP provides appropriate T cell receptor-mediated signalingThe NF-κB/Jurkat/GFP provides appropriate T cell receptor-mediated signaling

FAQs

Resources

Citations

  • Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
  • Pandi, K, et al. (2023) Porphyromonas gingivalis induction of TLR2 association with Vinculin enables PI3K activation and immune evasion. PLoS pathogens. 2023; 19(4):e1011284. PM ID: 37023213
  • Ramachandran, M, et al. (2023) Tailoring vascular phenotype through AAV therapy promotes anti-tumor immunity in glioma. Cancer cell. 2023;. PM ID: 37172581
  • Wen, YC, et al. (2023) CHRM4/AKT/MYCN upregulates interferon alpha-17 in the tumor microenvironment to promote neuroendocrine differentiation of prostate cancer. Cell death & disease. 2023; 14(5):304. PM ID: 37142586
  • Li, X, et al. (2023) Rosmarinic acid ameliorates autoimmune responses through suppression of intracellular nucleic acid-mediated type I interferon expression. Biochemical and Biophysical Research Communications. 2023;. Link: Biochemical and Biophysical Research Communications
  • Ibrahim, L, et al. (2023) Succinylation of a KEAP1 sensor lysine promotes NRF2 activation. bioRxiv : the preprint server for biology. 2023;. PM ID: 37215033
  • Park, CS, et al. (2023) Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia. Cell reports. 2023; 42(7):112804. PM ID: 37453060
  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
  • Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009
  • Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 2022;. PM ID: 36266527
  • Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 2022;:100191. Link: Cell Genomics
  • Caligiuri, SPB, et al. (2022) Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences of the United States of America. 2022; 119(46):e2209870119. PM ID: 36346845
  • Tan, TG, et al. (2022) SPATA2 and CYLD inhibit T cell infiltration into colorectal cancer via regulation of IFN-γ/STAT1 axis. Frontiers in oncology. 2022; 12:1016307. PM ID: 36531014
  • Mauro-Lizcano, M, Sotgia, F & Lisanti, MP. (2022) SOX2-high cancer cells exhibit an aggressive phenotype, with increases in stemness, proliferation and invasion, as well as higher metabolic activity and ATP production. Aging. 2022; 14(24):9877-9889. PM ID: 36566021
  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 2021;. PM ID: 33545065

Products

Catalog Number Description Size Price Quantity Add to Cart
TR850A-1 NF-kB/Jurkat/GFP Transcriptional Reporter Cell Line 2 x 10^6 Cells $3709
- +

Overview

Overview

Monitor NF-κB signaling in Jurkat cells

Speed your studies with this pre-built Jurkat cell line for monitoring NF-κB signaling in real time. We’ve already integrated an expression cassette that includes NF-κB-responsive transcriptional elements upstream of a minimal CMV promoter (mCMV)-GFP cassette. Expression of GFP (up to 30-fold over background) only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway.

  • Study NF-κB signaling in physiologically-relevant Jurkat cells
  • Use for fluorescence microscopy or FACS
  • Take advantage of the cell line’s 30-fold over background NF-κB-dependent GFP expression
  • Compatible with a variety of screening methods including small molecule and RNAi

How It Works

Supporting Data

Supporting Data

See the NF-κB/Jurkat/GFP Transcriptional Reporter Cell Line in action

Strong GFP fluorescence in response to signaling enables FACS

Strong GFP fluorescence in response to signaling enables FACS

The NF-κB/Jurkat/GFP provides a strong, dose-dependent response to TNF-αThe NF-κB/Jurkat/GFP provides a strong, dose-dependent response to TNF-α

METHODS: NF-B/Jurkat/GFP™ Reporter cells (5×105 cells) were plated at a concentration of 1 million cells/mL into each well of a 24-well plate. TNF-α was added in the amount indicated in the figure. After 24 hours, GFP fluorescence was measured and intensities plotted against TNF-α concentration (A). The fluorescent cells in the original 24-well plate were also photographed on a Zeiss inverted epi-fluorescence microscope (B). Alternatively, 200 μL of cells were fixed with formaldehyde and GFP reporter induction analyzed by flow cytometry, and either the GFP intensities (C) or the percentage of GFP positive cells (D) were plotted against the amount of TNF-α.

The NF-κB/Jurkat/GFP provides appropriate T cell receptor-mediated signalingThe NF-κB/Jurkat/GFP provides appropriate T cell receptor-mediated signaling

FAQs

Citations

  • Ishino, T, et al. (2023) Somatic mutations can induce a noninflamed tumour microenvironment via their original gene functions, despite deriving neoantigens. British journal of cancer. 2023;. PM ID: 36732592
  • Pandi, K, et al. (2023) Porphyromonas gingivalis induction of TLR2 association with Vinculin enables PI3K activation and immune evasion. PLoS pathogens. 2023; 19(4):e1011284. PM ID: 37023213
  • Ramachandran, M, et al. (2023) Tailoring vascular phenotype through AAV therapy promotes anti-tumor immunity in glioma. Cancer cell. 2023;. PM ID: 37172581
  • Wen, YC, et al. (2023) CHRM4/AKT/MYCN upregulates interferon alpha-17 in the tumor microenvironment to promote neuroendocrine differentiation of prostate cancer. Cell death & disease. 2023; 14(5):304. PM ID: 37142586
  • Li, X, et al. (2023) Rosmarinic acid ameliorates autoimmune responses through suppression of intracellular nucleic acid-mediated type I interferon expression. Biochemical and Biophysical Research Communications. 2023;. Link: Biochemical and Biophysical Research Communications
  • Ibrahim, L, et al. (2023) Succinylation of a KEAP1 sensor lysine promotes NRF2 activation. bioRxiv : the preprint server for biology. 2023;. PM ID: 37215033
  • Park, CS, et al. (2023) Stromal-induced epithelial-mesenchymal transition induces targetable drug resistance in acute lymphoblastic leukemia. Cell reports. 2023; 42(7):112804. PM ID: 37453060
  • Labanieh, L, et al. (2022) Enhanced safety and efficacy of protease-regulated CAR-T cell receptors. Cell. 2022;. PM ID: 35483375
  • Teng, CT, et al. (2022) SUPPLEMENTARY MATERIAL: Development of novel cell lines for high throughput screening to detect estrogen-related receptor alpha modulators. slas-discovery.org. 2022;. Link: slas-discovery.org
  • Dane, EL, et al. (2022) STING agonist delivery by tumour-penetrating PEG-lipid nanodiscs primes robust anticancer immunity. Nature materials. 2022; 21(6):710-720. PM ID: 35606429
  • Liu, Y, et al. (2022) MCTP1 promotes SNAI1-driven neuroendocrine differentiation and epithelial-to- mesenchymal transition of prostate cancer enhancement by ZBTB46/FOXA2/HIF1A. Research Square. 2022;. Link: Research Square
  • Deng, Z, Lyu, W & Zhang, G. (2022) High-Throughput Identification of Epigenetic Compounds to Enhance Chicken Host Defense Peptide Gene Expression. Antibiotics (Basel, Switzerland). 2022; 11(7). PM ID: 35884187
  • Chang, WM, et al. (2022) The aberrant cancer metabolic gene carbohydrate sulfotransferase 11 promotes non-small cell lung cancer cell metastasis via dysregulation of ceruloplasmin and intracellular iron balance. Translational oncology. 2022; 25:101508. PM ID: 35985204
  • Chen, C, et al. (2022) ATF4-dependent fructolysis fuels growth of glioblastoma multiforme. Nature communications. 2022; 13(1):6108. PM ID: 36245009
  • Takase, S, et al. (2022) 17β-neriifolin from unripe fruits of Cerbera manghas suppressed cell proliferation via the inhibition of HOXA9-dependent transcription and the induction of apoptosis in the human AML cell line THP-1. Journal of natural medicines. 2022;. PM ID: 36266527
  • Donohue, L, et al. (2022) A cis-regulatory lexicon of DNA motif combinations mediating cell-type-specific gene regulation. Cell Genomics. 2022;:100191. Link: Cell Genomics
  • Caligiuri, SPB, et al. (2022) Hedgehog-interacting protein acts in the habenula to regulate nicotine intake. Proceedings of the National Academy of Sciences of the United States of America. 2022; 119(46):e2209870119. PM ID: 36346845
  • Tan, TG, et al. (2022) SPATA2 and CYLD inhibit T cell infiltration into colorectal cancer via regulation of IFN-γ/STAT1 axis. Frontiers in oncology. 2022; 12:1016307. PM ID: 36531014
  • Mauro-Lizcano, M, Sotgia, F & Lisanti, MP. (2022) SOX2-high cancer cells exhibit an aggressive phenotype, with increases in stemness, proliferation and invasion, as well as higher metabolic activity and ATP production. Aging. 2022; 14(24):9877-9889. PM ID: 36566021
  • Haag, D, et al. (2021) H3.3-K27M drives neural stem cell-specific gliomagenesis in a human iPSC-derived model. Cancer cell. 2021;. PM ID: 33545065