Exo-FBS

Bovine exosome-depleted FBS, for isolating exosomes from cultured cells without the complication of bovine exosomes that are present in conventional FBS.
  • Exosome-sized vesicles removed
  • Very low levels of CD63-positive bovine exosomes
  • Undetectable levels of bovine miRNAs
  • Comparable growth rates as standard FBS
  • Interchangeable with standard FBS (add 10% in DMEM or RPMI)

Products

Catalog Number Description Size Price Quantity Add to Cart
EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250 mL $821
- +
EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement 50 mL $189
- +

Overview

Overview

Avoid inadvertently studying bovine exosomes

Fetal bovine serum, or FBS, is an important component of many types of cell culture media. But for researchers interested in isolating exosomes from cultured cells, standard FBS can introduce unwanted complications—bovine exosomes, which can cause significant background issues or interfere with functional studies. Which is why SBI developed Exo-FBS, our patented exosome-depleted FBS.
  • Exosome-sized vesicles removed
  • Very low levels of CD63-positive bovine exosomes
  • Undetectable levels of bovine miRNAs
  • Comparable growth rates as standard FBS
  • Interchangeable with standard FBS (add 10% in DMEM or RPMI)

How It Works

Supporting Data

Supporting Data

High quality and great performance

Exo-FBS supports robust cell growth (Figure 1), has greatly reduced levels of bovine exosomes (Figures 2), and bovine miRNAs (Figure 3). Cell growth in media supplemented with Exo-FBS is similar to cell growth in media supplemented with standard FBS (Figure 1).

Exo-FBS supports more robust cell growth health than competitors

Exo-FBS supports more robust cell growth than competitors

Figure 1. Exo-FBS supports more robust cell growth than Company G’s exosome-depleted FBS. HepG2 cells were grown in media containing either standard FBS (control), Exo-FBS, or Company G’s exosome-depleted FBS. Data is shown after one and two passages and illustrates that Exo-FBS provides a higher number of viable cells (left panel, viability assessed using a CCK-8 assay) and a healthier cell morphology (right panel) than Company G’s product.

 

 

anti-CD63 Elisa shows low levels of exosomes in Exo-FBS

 

Figure 2. Bovine α-CD63 ELISA shows low levels of exosomes in Exo-FBS CD63 is an exosome-specific marker. An α-CD63 ELISA of standard FBS and Exo-FBS shows very low levels of CD63 in Exo-FBS, supporting the NTA data, which showed low numbers of exosome-sized particles in Exo-FBS (Figure 1). Equal volumes (50 µl) of either standard FBS or Exo-FBS depleted media supplement were used and the graphed results normalized to the signal level of standard FBS.

 

qPCR shows bovine miRNA is undetectable in Exo-FBS

 

Figure 3. qPCR assays show undetectable levels of bovine exosomal miRNAs in Exo-FBS. While standard FBS contains amplifiable miRNAs (12 of the 72 individual miRNAs tested, left panels), Exo-FBS shows no amplifiable miRNAs (right panels). Standard FBS and Exo-FBS media supplements (4 ml) were treated with Trizol extraction methods to recover exosomal RNAs. RNA was converted to cDNA and 72 individual bovine microRNAs were measured by qPCR using SBI’s QuantiMir system.

 

 

Resources

Citations

  • Wu, Q, et al. (2023) Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair. Bioactive Materials. 1970 Jan 1; 20:208-220. Link: Bioactive Materials
  • Lu, R, et al. (2023) Fatty hepatocyte-derived exosomal miR-122 promotes lipid synthesis and reduces immunocompetence in grass carp (Ctenopharyngodon idella). Aquaculture. 1970 Jan 1; 563:738921. Link: Aquaculture
  • He, L, et al. (2022) A novel specific and ultrasensitive method detecting extracellular vesicles secreted from lung cancer by padlock probe-based exponential rolling circle amplification. Nano Today. 1970 Jan 1; 42:101334. Link: Nano Today
  • Gaines, D & Nestorova, G. (2022) Extracellular vesicles-derived microRNAs expression as biomarkers for neurological radiation injury: Risk assessment for space exploration. Life Sciences in Space Research. 1970 Jan 1; 32:54-62. Link: Life Sciences in Space Research
  • Yang, M, et al. (2022) Extracellular vesicle glucose transporter-1 and glycan features in monocyte-endothelial inflammatory interactions. Nanomedicine : nanotechnology, biology, and medicine. 1970 Jan 1;:102515. PM ID: 35074500
  • Wang, X, et al. (2022) CircFBXW8 Acts an Oncogenic Role in the Malignant Progression of Non-small Cell Lung Carcinoma by miR-370-3p-Dependent Regulation of TRIM44. Biochemical genetics. 1970 Jan 1;. PM ID: 34988777
  • Kanlikilicer, P. (2022) Exosome-related Methods and Potential Use as Vaccines. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2435:35-41. PM ID: 34993938
  • Busatto, S, et al. (2022) Considerations for extracellular vesicle and lipoprotein interactions in cell culture assays. Journal of extracellular vesicles. 1970 Jan 1; 11(4):e12202. PM ID: 35362268
  • Tung, MC, et al. (2022) Targeting DRD2 by the antipsychotic drug, penfluridol, retards growth of renal cell carcinoma via inducing stemness inhibition and autophagy-mediated apoptosis. Cell death & disease. 1970 Jan 1; 13(4):400. PM ID: 35461314
  • Marques de Menezes, EG, et al. (2022) Plasma CD16+ Extracellular Vesicles Associate with Carotid Artery Intima-Media Thickness in HIV+ Adults on Combination Antiretroviral Therapy. mBio. 1970 Jan 1;:e0300521. PM ID: 35435733
  • Shibamoto, J, et al. (2022) Removal of small extracellular vesicles inhibits the progression of peritoneal dissemination in gastric cancer. Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association. 1970 Jan 1;. PM ID: 35368210
  • Ampudia-Mesias, E, et al. (2022) SRPX Emerges as a Potential Tumor Marker in the Extracellular Vesicles of Glioblastoma. Cancers. 1970 Jan 1; 14(8). PM ID: 35454889
  • Pane, K, et al. (2022) Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study. International journal of molecular sciences. 1970 Jan 1; 23(7). PM ID: 35409352
  • Furuke, H, et al. (2022) miR‑4730 suppresses the progression of liver cancer by targeting the high mobility group A1 pathway. International journal of molecular medicine. 1970 Jan 1; 49(6). PM ID: 35485281
  • Choi, SY, Hong, SH & Lee, HJ. (2022) Differential expression and sorting of exosomal microRNAs upon activation of the human monocyte-like cell line U937. Biochemical and biophysical research communications. 1970 Jan 1; 610:147-153. PM ID: 35462096
  • Husna, AA, et al. (2022) Long non-coding RNA and transfer RNA-derived small fragments in exosomes are potential biomarkers for canine oral melanoma. Veterinary and comparative oncology. 1970 Jan 1;. PM ID: 35388611
  • Derkus, B, et al. (2022) Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Advanced biology. 1970 Jan 1;:e2101317. PM ID: 35347890
  • Huang, N, et al. (2022) Plasma Exosomes Confer Hypoxic Pulmonary Hypertension by Transferring LOX-1 Cargo. Authorea . 1970 Jan 1;. Link: Authorea 
  • Al Faruque, H, et al. (2022) Enhanced effect of autologous EVs delivering paclitaxel in pancreatic cancer. Journal of controlled release : official journal of the Controlled Release Society. 1970 Jan 1; 347:330-346. PM ID: 35561870
  • Almeria, C, et al. (2022) Heterogeneity of mesenchymal stem cell-derived extracellular vesicles is highly impacted by the tissue/cell source and culture conditions. Cell & bioscience. 1970 Jan 1; 12(1):51. PM ID: 35501833

Products

Catalog Number Description Size Price Quantity Add to Cart
EXO-FBS-250A-1 Exosome-depleted FBS Media Supplement 250 mL $821
- +
EXO-FBS-50A-1 Exosome-depleted FBS Media Supplement 50 mL $189
- +

Overview

Overview

Avoid inadvertently studying bovine exosomes

Fetal bovine serum, or FBS, is an important component of many types of cell culture media. But for researchers interested in isolating exosomes from cultured cells, standard FBS can introduce unwanted complications—bovine exosomes, which can cause significant background issues or interfere with functional studies. Which is why SBI developed Exo-FBS, our patented exosome-depleted FBS.
  • Exosome-sized vesicles removed
  • Very low levels of CD63-positive bovine exosomes
  • Undetectable levels of bovine miRNAs
  • Comparable growth rates as standard FBS
  • Interchangeable with standard FBS (add 10% in DMEM or RPMI)

How It Works

Supporting Data

Supporting Data

High quality and great performance

Exo-FBS supports robust cell growth (Figure 1), has greatly reduced levels of bovine exosomes (Figures 2), and bovine miRNAs (Figure 3). Cell growth in media supplemented with Exo-FBS is similar to cell growth in media supplemented with standard FBS (Figure 1).

Exo-FBS supports more robust cell growth health than competitors

Exo-FBS supports more robust cell growth than competitors

Figure 1. Exo-FBS supports more robust cell growth than Company G’s exosome-depleted FBS. HepG2 cells were grown in media containing either standard FBS (control), Exo-FBS, or Company G’s exosome-depleted FBS. Data is shown after one and two passages and illustrates that Exo-FBS provides a higher number of viable cells (left panel, viability assessed using a CCK-8 assay) and a healthier cell morphology (right panel) than Company G’s product.

 

 

anti-CD63 Elisa shows low levels of exosomes in Exo-FBS

 

Figure 2. Bovine α-CD63 ELISA shows low levels of exosomes in Exo-FBS CD63 is an exosome-specific marker. An α-CD63 ELISA of standard FBS and Exo-FBS shows very low levels of CD63 in Exo-FBS, supporting the NTA data, which showed low numbers of exosome-sized particles in Exo-FBS (Figure 1). Equal volumes (50 µl) of either standard FBS or Exo-FBS depleted media supplement were used and the graphed results normalized to the signal level of standard FBS.

 

qPCR shows bovine miRNA is undetectable in Exo-FBS

 

Figure 3. qPCR assays show undetectable levels of bovine exosomal miRNAs in Exo-FBS. While standard FBS contains amplifiable miRNAs (12 of the 72 individual miRNAs tested, left panels), Exo-FBS shows no amplifiable miRNAs (right panels). Standard FBS and Exo-FBS media supplements (4 ml) were treated with Trizol extraction methods to recover exosomal RNAs. RNA was converted to cDNA and 72 individual bovine microRNAs were measured by qPCR using SBI’s QuantiMir system.

 

 

Citations

  • Wu, Q, et al. (2023) Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair. Bioactive Materials. 1970 Jan 1; 20:208-220. Link: Bioactive Materials
  • Lu, R, et al. (2023) Fatty hepatocyte-derived exosomal miR-122 promotes lipid synthesis and reduces immunocompetence in grass carp (Ctenopharyngodon idella). Aquaculture. 1970 Jan 1; 563:738921. Link: Aquaculture
  • He, L, et al. (2022) A novel specific and ultrasensitive method detecting extracellular vesicles secreted from lung cancer by padlock probe-based exponential rolling circle amplification. Nano Today. 1970 Jan 1; 42:101334. Link: Nano Today
  • Gaines, D & Nestorova, G. (2022) Extracellular vesicles-derived microRNAs expression as biomarkers for neurological radiation injury: Risk assessment for space exploration. Life Sciences in Space Research. 1970 Jan 1; 32:54-62. Link: Life Sciences in Space Research
  • Yang, M, et al. (2022) Extracellular vesicle glucose transporter-1 and glycan features in monocyte-endothelial inflammatory interactions. Nanomedicine : nanotechnology, biology, and medicine. 1970 Jan 1;:102515. PM ID: 35074500
  • Wang, X, et al. (2022) CircFBXW8 Acts an Oncogenic Role in the Malignant Progression of Non-small Cell Lung Carcinoma by miR-370-3p-Dependent Regulation of TRIM44. Biochemical genetics. 1970 Jan 1;. PM ID: 34988777
  • Kanlikilicer, P. (2022) Exosome-related Methods and Potential Use as Vaccines. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2435:35-41. PM ID: 34993938
  • Busatto, S, et al. (2022) Considerations for extracellular vesicle and lipoprotein interactions in cell culture assays. Journal of extracellular vesicles. 1970 Jan 1; 11(4):e12202. PM ID: 35362268
  • Tung, MC, et al. (2022) Targeting DRD2 by the antipsychotic drug, penfluridol, retards growth of renal cell carcinoma via inducing stemness inhibition and autophagy-mediated apoptosis. Cell death & disease. 1970 Jan 1; 13(4):400. PM ID: 35461314
  • Marques de Menezes, EG, et al. (2022) Plasma CD16+ Extracellular Vesicles Associate with Carotid Artery Intima-Media Thickness in HIV+ Adults on Combination Antiretroviral Therapy. mBio. 1970 Jan 1;:e0300521. PM ID: 35435733
  • Shibamoto, J, et al. (2022) Removal of small extracellular vesicles inhibits the progression of peritoneal dissemination in gastric cancer. Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association. 1970 Jan 1;. PM ID: 35368210
  • Ampudia-Mesias, E, et al. (2022) SRPX Emerges as a Potential Tumor Marker in the Extracellular Vesicles of Glioblastoma. Cancers. 1970 Jan 1; 14(8). PM ID: 35454889
  • Pane, K, et al. (2022) Comparative Proteomic Profiling of Secreted Extracellular Vesicles from Breast Fibroadenoma and Malignant Lesions: A Pilot Study. International journal of molecular sciences. 1970 Jan 1; 23(7). PM ID: 35409352
  • Furuke, H, et al. (2022) miR‑4730 suppresses the progression of liver cancer by targeting the high mobility group A1 pathway. International journal of molecular medicine. 1970 Jan 1; 49(6). PM ID: 35485281
  • Choi, SY, Hong, SH & Lee, HJ. (2022) Differential expression and sorting of exosomal microRNAs upon activation of the human monocyte-like cell line U937. Biochemical and biophysical research communications. 1970 Jan 1; 610:147-153. PM ID: 35462096
  • Husna, AA, et al. (2022) Long non-coding RNA and transfer RNA-derived small fragments in exosomes are potential biomarkers for canine oral melanoma. Veterinary and comparative oncology. 1970 Jan 1;. PM ID: 35388611
  • Derkus, B, et al. (2022) Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure. Advanced biology. 1970 Jan 1;:e2101317. PM ID: 35347890
  • Huang, N, et al. (2022) Plasma Exosomes Confer Hypoxic Pulmonary Hypertension by Transferring LOX-1 Cargo. Authorea . 1970 Jan 1;. Link: Authorea 
  • Al Faruque, H, et al. (2022) Enhanced effect of autologous EVs delivering paclitaxel in pancreatic cancer. Journal of controlled release : official journal of the Controlled Release Society. 1970 Jan 1; 347:330-346. PM ID: 35561870
  • Almeria, C, et al. (2022) Heterogeneity of mesenchymal stem cell-derived extracellular vesicles is highly impacted by the tissue/cell source and culture conditions. Cell & bioscience. 1970 Jan 1; 12(1):51. PM ID: 35501833