QuantiMir™ Kit

Quickly and easily quantify miRNAs with this kit that efficiently converts miRNA into cDNA for qPCR assays

Products

Catalog Number Description Size Price Quantity Add to Cart
RA420A-1 QuantiMir™ Kit 20 Reactions $563.00
- +
Contact Us

Overview

Overview

Get your miRNA ready for qPCR profiling When you’d like to profile miRNA with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
  • Simple and robust procedure
  • Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
  • Suitable for high-throughput screening of clinical samples
  • Sensitive and accurate (see the data in the Supporting Data section below)
  • Versatile—design your own miRNA assays
Each kit includes three reference assays—human U6, mouse U6 and human/mouse miR-16. We also offer the following reference assays as individual 50-assay kits:
Catalog #Amplified small RNASpecies compatibility
RA420A-hU6Human U6Human
RA420A-miR-16miR-16Human, Mouse
RA420A-mU6Mouse U6Mouse
RA420A-RNU1RNU1 (U1)Human, Mouse, Rat, Monkey
RA420A-RNU43RNU43Human, Mouse, Rat
RA420A-rU6Rat U6Rat

How It Works

How It Works

The QuantiMir Kit enables robust miRNA quantitation through a simple and efficient workflow

Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.

  • Tag all small RNAs with a poly-A tail
  • Anneal an oligo-dT adaptor
  • Reverse transcribe to create first-strand cDNA

The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.

Create custom miRNA assays

To design your own miRNA assays, simply synthesize an oligo using the sequence of the mature miRNA you’d like to profile as the forward primer in your miRNA qPCR assay, and use with the universal reverse primer included in the QuantiMir Kit.

Supporting Data

Supporting Data

Converting miRNA into cDNA for accurate qPCR profiling and quantitation

The QuantiMir Kit is sensitive

The QuantiMir Kit is highly sensitive and enables measurement across a wide dynamic range

Figure 1. The QuantiMir Kit is highly sensitive and enables measurement across a wide dynamic range. (A) Starting with total, Trizol-extracted RNA or fractionated small RNA samples you can measure from several micrograms down to picograms of input RNA with excellent accuracy. (B) You can also detect differential miRNA expression across a dynamic range of at least 6 logs.

The QuantiMir Kit is accurateThe QuantiMir Kit is accurate

Figure 2. The QuantiMir Kit is accurate. We used the QuantiMir Kit to synthesize first strand cDNAs from 18 different human tissues: adipose, bladder, brain, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testes, and thymus. The cDNAs were balanced to yield equal Ct values for the U6 snRNA normalizing transcript (bottom left plot, green bars). Real-time PCR results demonstrate that the normalizing snRNA is uniformly expressed across the 18 tissues examined. As expected, assays for miR-1 are show specific expression in heart and musculoskeletal tissues (top left plot, red bars) whereas assays for miR-122 show specific expression in the liver (top right plot, blue bar).

Use the QuantiMir Kit to profile miRNAs from cancerous and normal tissuesUse the QuantiMir Kit to profile miRNAs from cancerous and normal tissues

Figure 3. Use the QuantiMir Kit to profile miRNAs from cancerous and normal tissues. Example of quantitative miRNA profiling of nine miRNAs in five different normal and tumor-derived samples.

Measure both siRNA and mRNA knockdown in a single QuantiMir cDNA sampleMeasure both siRNA and mRNA knockdown in a single QuantiMir cDNA sample

Figure 4. Measure both siRNA and mRNA knockdown in a single QuantiMir cDNA sample. A time-course using anti-p53 shRNA-directed knockdown of the endogenous p53 mRNA transcript demonstrates how the QuantiMir Kit can be used to measure both p53 siRNA (orange bars) and p53 mRNA (blue line) via qPCR.

Resources

Citations

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Products

Catalog Number Description Size Price Quantity Add to Cart
RA420A-1 QuantiMir™ Kit 20 Reactions $563.00
- +
Contact Us

Overview

Overview

Get your miRNA ready for qPCR profiling When you’d like to profile miRNA with qPCR, SBI’s QuantiMir™ Kit can get your samples ready. By efficiently converting all of your miRNA into cDNA, you can get accurate, sensitive, and quantitative qPCR data on the miRs in your sample. Great for understanding differential miRNA expression, simultaneously profiling siRNA and mRNA, and more.
  • Simple and robust procedure
  • Rapidly and efficiently convert all small RNAs into cDNAs for qPCR
  • Suitable for high-throughput screening of clinical samples
  • Sensitive and accurate (see the data in the Supporting Data section below)
  • Versatile—design your own miRNA assays
Each kit includes three reference assays—human U6, mouse U6 and human/mouse miR-16. We also offer the following reference assays as individual 50-assay kits:
Catalog #Amplified small RNASpecies compatibility
RA420A-hU6Human U6Human
RA420A-miR-16miR-16Human, Mouse
RA420A-mU6Mouse U6Mouse
RA420A-RNU1RNU1 (U1)Human, Mouse, Rat, Monkey
RA420A-RNU43RNU43Human, Mouse, Rat
RA420A-rU6Rat U6Rat

How It Works

How It Works

The QuantiMir Kit enables robust miRNA quantitation through a simple and efficient workflow

Highly efficient poly-A tailing and reverse transcription in a single reaction tube provides uniform cDNA synthesis of miRNAs. The optimized reaction conditions and buffer components maximize cDNA yield when starting with several micrograms down to picograms of input total RNA. The universal 3′ tag sequence incorporated during reverse transcription enables easily scalable and accurate miRNA expression analysis by qPCR—profile thousands of different miRNAs from a single reverse transcription reaction.

  • Tag all small RNAs with a poly-A tail
  • Anneal an oligo-dT adaptor
  • Reverse transcribe to create first-strand cDNA

The result is a cDNA pool of anchor-tailed miRNAs that are ready for qPCR.

Create custom miRNA assays

To design your own miRNA assays, simply synthesize an oligo using the sequence of the mature miRNA you’d like to profile as the forward primer in your miRNA qPCR assay, and use with the universal reverse primer included in the QuantiMir Kit.

Supporting Data

Supporting Data

Converting miRNA into cDNA for accurate qPCR profiling and quantitation

The QuantiMir Kit is sensitive

The QuantiMir Kit is highly sensitive and enables measurement across a wide dynamic range

Figure 1. The QuantiMir Kit is highly sensitive and enables measurement across a wide dynamic range. (A) Starting with total, Trizol-extracted RNA or fractionated small RNA samples you can measure from several micrograms down to picograms of input RNA with excellent accuracy. (B) You can also detect differential miRNA expression across a dynamic range of at least 6 logs.

The QuantiMir Kit is accurateThe QuantiMir Kit is accurate

Figure 2. The QuantiMir Kit is accurate. We used the QuantiMir Kit to synthesize first strand cDNAs from 18 different human tissues: adipose, bladder, brain, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testes, and thymus. The cDNAs were balanced to yield equal Ct values for the U6 snRNA normalizing transcript (bottom left plot, green bars). Real-time PCR results demonstrate that the normalizing snRNA is uniformly expressed across the 18 tissues examined. As expected, assays for miR-1 are show specific expression in heart and musculoskeletal tissues (top left plot, red bars) whereas assays for miR-122 show specific expression in the liver (top right plot, blue bar).

Use the QuantiMir Kit to profile miRNAs from cancerous and normal tissuesUse the QuantiMir Kit to profile miRNAs from cancerous and normal tissues

Figure 3. Use the QuantiMir Kit to profile miRNAs from cancerous and normal tissues. Example of quantitative miRNA profiling of nine miRNAs in five different normal and tumor-derived samples.

Measure both siRNA and mRNA knockdown in a single QuantiMir cDNA sampleMeasure both siRNA and mRNA knockdown in a single QuantiMir cDNA sample

Figure 4. Measure both siRNA and mRNA knockdown in a single QuantiMir cDNA sample. A time-course using anti-p53 shRNA-directed knockdown of the endogenous p53 mRNA transcript demonstrates how the QuantiMir Kit can be used to measure both p53 siRNA (orange bars) and p53 mRNA (blue line) via qPCR.

Citations

We're sorry, an error has occurred while generating this content.