Exo-FBS, heat inactivated

Bovine exosome-free FBS that has been heat inactivated, for labs that would like to use Exo-FBS but require heat inactivation.
  • Heat inactivated using a highly quality-controlled process
  • Exosome-sized vesicles removed
  • Very low levels of CD63-positive cow exosomes
  • Undetectable levels of cow miRNAs
  • Comparable growth rates as standard FBS

Products

Catalog Number Description Size Price Quantity Add to Cart
EXO-FBSHI-250A-1 Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified       250 mL $881
- +
EXO-FBSHI-50A-1 Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified       50 mL $214
- +

Overview

Overview

Avoid inadvertently studying bovine exosomes with heat inactivated Exo-FBS

Fetal bovine serum, or FBS, is an important component of many types of cell culture media, and some researchers require heat inactivated FBS. But for researchers interested in isolating exosomes from cultured cells, standard heat inactivated FBS can introduce unwanted complications—bovine exosomes, which can cause significant background issues or interfere with functional studies. Which is why SBI developed heat inactivated Exo-FBS, our patented exosome-depleted FBS.

  • Heat inactivated using a highly quality-controlled process
  • Exosome-sized vesicles removed
  • Very low levels of CD63-positive cow exosomes
  • Undetectable levels of cow miRNAs
  • Comparable growth rates as standard FBS
  • Interchangeable with standard FBS (add 10% in DMEM or RPMI)

How It Works

Supporting Data

Supporting Data

High quality and great performance

Exo-FBS has greatly reduced levels of bovine exosomes (Figures 1 and 2), bovine miRNAs (Figure 3), and is even cleaner than ultracentrifuged FBS (Figure 4). Cell growth in media supplemented with Exo-FBS is similar to cell growth in media supplemented with standard FBS (Figure 5).

Exo-FBS has greatly reduced levels of bovine exosomes

NanoSight shows low levels of exosomes in Exo-FBS

Figure 1. NanoSight particle analysis shows low levels of exosomes in Exo-FBS. While standard FBS contains exosome-sized particles (top panels), Exo-FBS shows almost no particles (bottom panels). Standard FBS and Exo-FBS samples were diluted 1:1000 and then analyzed for particle size and abundance using a NanoSight LM10 instrument.

anti-CD63 Elisa shows low levels of exosomes in Exo-FBS

Figure 2. Bovine α-CD63 ELISA shows low levels of exosomes in Exo-FBS CD63 is an exosome-specific marker. An α-CD63 ELISA of standard FBS and Exo-FBS shows very low levels of CD63 in Exo-FBS, supporting the NanoSight particle analysis which showed low numbers of exosome-sized particles in Exo-FBS (Figure 1). Equal volumes (50 µl) of either standard FBS or Exo-FBS depleted media supplement were used and the graphed results normalized to the signal level of standard FBS.

qPCR shows bovine miRNA is undetectable in Exo-FBS

Figure 3. qPCR assays show undetectable levels of bovine exosomal miRNAs in Exo-FBS. While standard FBS contains amplifiable miRNAs (12 of the 72 individual miRNAs tested, left panels), Exo-FBS shows no amplifiable miRNAs (right panels). Standard FBS and Exo-FBS media supplements (4 ml) were treated with Trizol extraction methods to recover exosomal RNAs. RNA was converted to cDNA and 72 individual bovine microRNAs were measured by qPCR using SBI’s QuantiMir system.

NanoSight shows Exo-FBS is cleaner than ultracentrifuged FBS

Figure 4. NanoSight analysis shows Exo-FBS is cleaner than ultracentrifuged FBS. Quality Control data is generated on every batch of Exo-FBS produced at SBI by comparing NanoSight particle count analyses to the source FBS, FBS ultracentrifuged for 18 hours, and Exo-FBS. All samples were diluted 1:100 and data collected in triplicate.

Exo-FBS supports comparable cell growth to FBS

Figure 5. Cells grown in 10% Exo-FBS show comparable growth rates to 10% standard FBS. HT1080 fibrosarcoma cells, PC-3 prostate cancer cells, MCF-7 breast cancer cells, and HEK293 cells were seeded at either 10,000 or 20,000 cells and then cultured under standard conditions at 37°C with 5% CO2 for 5 days.

FAQs

Resources

Citations

  • Liu, J, et al. (2024) Exosomes derived from impaired liver aggravate alveolar bone loss via shuttle of Fasn in type 2 diabetes mellitus. Bioactive materials. 2024; 33:85-99. PM ID: 38024229
  • Li, X, et al. (2024) The association of SPARC with hypertension and its function in endothelial-dependent relaxation. Atherosclerosis. 2024; 388:117390. Link: Atherosclerosis
  • Kang, W, et al. (2024) Lyoprotectant Constituents Suited for Lyophilization and Reconstitution of Stem-Cell-Derived Extracellular Vesicles. Biomaterials Research. 2024; 28. Link: Biomaterials Research
  • Qi, L, et al. (2024) Mesoporous bioactive glass scaffolds for the delivery of bone marrow stem cell-derived osteoinductive extracellular vesicles lncRNA promote senescent bone defect repair by targeting the miR-1843a-5p/mob3a/YAP axis. Acta Biomaterialia. 2024;. Link: Acta Biomaterialia
  • Wang, W, et al. (2024) Integrin β1-rich extracellular vesicles of kidney recruit Fn1+ macrophages to aggravate ischemia-reperfusion-induced inflammation. JCI insight. 2024; 9(2). PM ID: 38258908
  • Liu, B, et al. (2024) DNA Logical Device Combining an Entropy-Driven Catalytic Amplification Strategy for the Simultaneous Detection of Exosomal Multiplex miRNAs In Situ. Analytical chemistry. 2024; 96(4):1733-1741. PM ID: 38227423
  • Ramil, CP, et al. (2024) Extracellular vesicles released by cancer-associated fibroblast-induced myeloid-derived suppressor cells inhibit T-cell function. Oncoimmunology. 2024; 13(1):2300882. PM ID: 38192443
  • Ueda, S, et al. (2024) Consistency between Primary Uterine Corpus Malignancies and Their Corresponding Patient-Derived Xenograft Models. International Journal of Molecular Sciences. 2024; 25(3):1486. Link: International Journal of Molecular Sciences
  • Li, B, et al. (2024) Characteristics of Inflammatory and Normal Endothelial Exosomes on Endothelial Function and the Development of Hypertension. Inflammation. 2024;. PM ID: 38240985
  • Luo, P, et al. (2024) Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Rescue Testicular Aging. Biomedicines. 2024; 12(1). PM ID: 38255205
  • Jin, Y, et al. (2024) The homologous tumor-derived-exosomes loaded with miR-1270 selectively enhanced the suppression effect for colorectal cancer cells. Cancer medicine. 2024; 13(1). PM ID: 38197582
  • Kashiwagi, R, Udono, M & Katakura, Y. (2024) Fructobacillus fructosus OS-1010 strain stimulates intestinal cells to secrete exosomes that activate muscle cells. Cytotechnology. 2024;. Link: Cytotechnology
  • Wu, Q, et al. (2023) Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair. Bioactive Materials. 2023; 20:208-220. Link: Bioactive Materials
  • Lu, R, et al. (2023) Fatty hepatocyte-derived exosomal miR-122 promotes lipid synthesis and reduces immunocompetence in grass carp (Ctenopharyngodon idella). Aquaculture. 2023; 563:738921. Link: Aquaculture
  • Sultana, H & Neelakanta, G. (2023) Isolation of Exosomes or Extracellular Vesicles from West Nile Virus-Infected N2a Cells, Primary Cortical Neurons, and Brain Tissues. Methods in molecular biology (Clifton, N.J.). 2023; 2585:79-95. PM ID: 36331767
  • Chattrairat, K, et al. (2023) All-in-One Nanowire Assay System for Capture and Analysis of Extracellular Vesicles from an ex Vivo Brain Tumor Model. ACS nano. 2023;. PM ID: 36655866
  • Zhou, C, et al. (2023) Water-soluble extracellular vesicle probes based on conjugated oligoelectrolytes. Science advances. 2023; 9(2):eade2996. PM ID: 36630497
  • Guan, M, et al. (2023) Exosome-laden injectable self-healing hydrogel based on quaternized chitosan and oxidized starch attenuates disc degeneration by suppressing nucleus pulposus senescence. International journal of biological macromolecules. 2023;:123479. PM ID: 36731695
  • Nikoloff, JM, Saucedo-Espinosa, MA & Dittrich, PS. (2023) Microfluidic Platform for Profiling of Extracellular Vesicles from Single Breast Cancer Cells. Analytical chemistry. 2023; 95(3):1933-1939. PM ID: 36608325
  • Fu, Y & Xiong, S. (2023) Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism. PLoS pathogens. 2023; 19(1):e1011090. PM ID: 36634130

Products

Catalog Number Description Size Price Quantity Add to Cart
EXO-FBSHI-250A-1 Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified       250 mL $881
- +
EXO-FBSHI-50A-1 Exosome-depleted FBS Media Supplement Heat Inactivated - USA Certified       50 mL $214
- +

Overview

Overview

Avoid inadvertently studying bovine exosomes with heat inactivated Exo-FBS

Fetal bovine serum, or FBS, is an important component of many types of cell culture media, and some researchers require heat inactivated FBS. But for researchers interested in isolating exosomes from cultured cells, standard heat inactivated FBS can introduce unwanted complications—bovine exosomes, which can cause significant background issues or interfere with functional studies. Which is why SBI developed heat inactivated Exo-FBS, our patented exosome-depleted FBS.

  • Heat inactivated using a highly quality-controlled process
  • Exosome-sized vesicles removed
  • Very low levels of CD63-positive cow exosomes
  • Undetectable levels of cow miRNAs
  • Comparable growth rates as standard FBS
  • Interchangeable with standard FBS (add 10% in DMEM or RPMI)

How It Works

Supporting Data

Supporting Data

High quality and great performance

Exo-FBS has greatly reduced levels of bovine exosomes (Figures 1 and 2), bovine miRNAs (Figure 3), and is even cleaner than ultracentrifuged FBS (Figure 4). Cell growth in media supplemented with Exo-FBS is similar to cell growth in media supplemented with standard FBS (Figure 5).

Exo-FBS has greatly reduced levels of bovine exosomes

NanoSight shows low levels of exosomes in Exo-FBS

Figure 1. NanoSight particle analysis shows low levels of exosomes in Exo-FBS. While standard FBS contains exosome-sized particles (top panels), Exo-FBS shows almost no particles (bottom panels). Standard FBS and Exo-FBS samples were diluted 1:1000 and then analyzed for particle size and abundance using a NanoSight LM10 instrument.

anti-CD63 Elisa shows low levels of exosomes in Exo-FBS

Figure 2. Bovine α-CD63 ELISA shows low levels of exosomes in Exo-FBS CD63 is an exosome-specific marker. An α-CD63 ELISA of standard FBS and Exo-FBS shows very low levels of CD63 in Exo-FBS, supporting the NanoSight particle analysis which showed low numbers of exosome-sized particles in Exo-FBS (Figure 1). Equal volumes (50 µl) of either standard FBS or Exo-FBS depleted media supplement were used and the graphed results normalized to the signal level of standard FBS.

qPCR shows bovine miRNA is undetectable in Exo-FBS

Figure 3. qPCR assays show undetectable levels of bovine exosomal miRNAs in Exo-FBS. While standard FBS contains amplifiable miRNAs (12 of the 72 individual miRNAs tested, left panels), Exo-FBS shows no amplifiable miRNAs (right panels). Standard FBS and Exo-FBS media supplements (4 ml) were treated with Trizol extraction methods to recover exosomal RNAs. RNA was converted to cDNA and 72 individual bovine microRNAs were measured by qPCR using SBI’s QuantiMir system.

NanoSight shows Exo-FBS is cleaner than ultracentrifuged FBS

Figure 4. NanoSight analysis shows Exo-FBS is cleaner than ultracentrifuged FBS. Quality Control data is generated on every batch of Exo-FBS produced at SBI by comparing NanoSight particle count analyses to the source FBS, FBS ultracentrifuged for 18 hours, and Exo-FBS. All samples were diluted 1:100 and data collected in triplicate.

Exo-FBS supports comparable cell growth to FBS

Figure 5. Cells grown in 10% Exo-FBS show comparable growth rates to 10% standard FBS. HT1080 fibrosarcoma cells, PC-3 prostate cancer cells, MCF-7 breast cancer cells, and HEK293 cells were seeded at either 10,000 or 20,000 cells and then cultured under standard conditions at 37°C with 5% CO2 for 5 days.

FAQs

Citations

  • Liu, J, et al. (2024) Exosomes derived from impaired liver aggravate alveolar bone loss via shuttle of Fasn in type 2 diabetes mellitus. Bioactive materials. 2024; 33:85-99. PM ID: 38024229
  • Li, X, et al. (2024) The association of SPARC with hypertension and its function in endothelial-dependent relaxation. Atherosclerosis. 2024; 388:117390. Link: Atherosclerosis
  • Kang, W, et al. (2024) Lyoprotectant Constituents Suited for Lyophilization and Reconstitution of Stem-Cell-Derived Extracellular Vesicles. Biomaterials Research. 2024; 28. Link: Biomaterials Research
  • Qi, L, et al. (2024) Mesoporous bioactive glass scaffolds for the delivery of bone marrow stem cell-derived osteoinductive extracellular vesicles lncRNA promote senescent bone defect repair by targeting the miR-1843a-5p/mob3a/YAP axis. Acta Biomaterialia. 2024;. Link: Acta Biomaterialia
  • Wang, W, et al. (2024) Integrin β1-rich extracellular vesicles of kidney recruit Fn1+ macrophages to aggravate ischemia-reperfusion-induced inflammation. JCI insight. 2024; 9(2). PM ID: 38258908
  • Liu, B, et al. (2024) DNA Logical Device Combining an Entropy-Driven Catalytic Amplification Strategy for the Simultaneous Detection of Exosomal Multiplex miRNAs In Situ. Analytical chemistry. 2024; 96(4):1733-1741. PM ID: 38227423
  • Ramil, CP, et al. (2024) Extracellular vesicles released by cancer-associated fibroblast-induced myeloid-derived suppressor cells inhibit T-cell function. Oncoimmunology. 2024; 13(1):2300882. PM ID: 38192443
  • Ueda, S, et al. (2024) Consistency between Primary Uterine Corpus Malignancies and Their Corresponding Patient-Derived Xenograft Models. International Journal of Molecular Sciences. 2024; 25(3):1486. Link: International Journal of Molecular Sciences
  • Li, B, et al. (2024) Characteristics of Inflammatory and Normal Endothelial Exosomes on Endothelial Function and the Development of Hypertension. Inflammation. 2024;. PM ID: 38240985
  • Luo, P, et al. (2024) Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes Rescue Testicular Aging. Biomedicines. 2024; 12(1). PM ID: 38255205
  • Jin, Y, et al. (2024) The homologous tumor-derived-exosomes loaded with miR-1270 selectively enhanced the suppression effect for colorectal cancer cells. Cancer medicine. 2024; 13(1). PM ID: 38197582
  • Kashiwagi, R, Udono, M & Katakura, Y. (2024) Fructobacillus fructosus OS-1010 strain stimulates intestinal cells to secrete exosomes that activate muscle cells. Cytotechnology. 2024;. Link: Cytotechnology
  • Wu, Q, et al. (2023) Modification of adipose mesenchymal stem cells-derived small extracellular vesicles with fibrin-targeting peptide CREKA for enhanced bone repair. Bioactive Materials. 2023; 20:208-220. Link: Bioactive Materials
  • Lu, R, et al. (2023) Fatty hepatocyte-derived exosomal miR-122 promotes lipid synthesis and reduces immunocompetence in grass carp (Ctenopharyngodon idella). Aquaculture. 2023; 563:738921. Link: Aquaculture
  • Sultana, H & Neelakanta, G. (2023) Isolation of Exosomes or Extracellular Vesicles from West Nile Virus-Infected N2a Cells, Primary Cortical Neurons, and Brain Tissues. Methods in molecular biology (Clifton, N.J.). 2023; 2585:79-95. PM ID: 36331767
  • Chattrairat, K, et al. (2023) All-in-One Nanowire Assay System for Capture and Analysis of Extracellular Vesicles from an ex Vivo Brain Tumor Model. ACS nano. 2023;. PM ID: 36655866
  • Zhou, C, et al. (2023) Water-soluble extracellular vesicle probes based on conjugated oligoelectrolytes. Science advances. 2023; 9(2):eade2996. PM ID: 36630497
  • Guan, M, et al. (2023) Exosome-laden injectable self-healing hydrogel based on quaternized chitosan and oxidized starch attenuates disc degeneration by suppressing nucleus pulposus senescence. International journal of biological macromolecules. 2023;:123479. PM ID: 36731695
  • Nikoloff, JM, Saucedo-Espinosa, MA & Dittrich, PS. (2023) Microfluidic Platform for Profiling of Extracellular Vesicles from Single Breast Cancer Cells. Analytical chemistry. 2023; 95(3):1933-1939. PM ID: 36608325
  • Fu, Y & Xiong, S. (2023) Exosomes mediate Coxsackievirus B3 transmission and expand the viral tropism. PLoS pathogens. 2023; 19(1):e1011090. PM ID: 36634130