ExoELISA-ULTRA Complete Kit (CD81 Detection)

With a 4-hour total assay time, this sensitive ELISA-based assay speeds quantitation of exosomes from most biofluids
  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD81
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications

Products

Catalog Number Description Size Price Quantity Add to Cart
EXEL-ULTRA-CD81-1 ExoELISA-ULTRA Complete Kit (CD81 detection) 96 Reactions $636
- +

Overview

Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD81 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Currently configured for detection of CD81, a common exosomal marker, ExoELISA-ULTRA CD81 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with common biofluids. The ExoELISA-ULTRA CD81 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NTA analysis and enables quantitation of exosomes carrying CD81 in your target samples. One ExoELISA-ULTRA CD81 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD81
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Choose the exosome quantitation method that’s best for your studies
 ExoELISA-ULTRA Complete KitsExoELISA KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor sensitive quantitation of exosomes when time and input sample are not limitingFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)Enzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)24 hours20 min60 min
Input sample amount (protein equivalent)1 – 200 µg>500 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCETFluoroCet

How It Works

Supporting Data

Supporting Data

The standard curve for ExoELISA-ULTRA CD81 shows robust linearity down to ~4 x 109 exosomes.

The standard curve for ExoELISA-ULTRA CD81 provides robust linearity down to ~4 x 10^9 exosomes.

Figure 1. ExoELISA-ULTRA CD81 standard curve shows robust linearity down to ~4 x 109 exosomes. OD450nm values of commonly used samples (e.g. human serum, HEK293T, and MCF7 breast cancer exosomes – in large colored dots) fall well within the standard curve for the assay.

Resources

Citations

Products

Catalog Number Description Size Price Quantity Add to Cart
EXEL-ULTRA-CD81-1 ExoELISA-ULTRA Complete Kit (CD81 detection) 96 Reactions $636
- +

Overview

Overview

Delivering ELISA-based exosome quantitation ULTRA fast

Improving on our popular ExoELISA Kits, the ExoELISA-ULTRA CD81 Kit increases the sensitivity of exosome detection—as low as 1 µg protein equivalent—while shortening the total assay time to only 4 hours.

Currently configured for detection of CD81, a common exosomal marker, ExoELISA-ULTRA CD81 is based on an ultra-sensitive, direct capture, colorimetric ELISA assay that is compatible with common biofluids. The ExoELISA-ULTRA CD81 Kit comes with an internal standard calibrated to exosomes from a range of biofluids. Calibration is achieved by NTA analysis and enables quantitation of exosomes carrying CD81 in your target samples. One ExoELISA-ULTRA CD81 Kit contains all of the necessary reagents (including assay plate) to perform up to 96 reactions.

  • Sensitive—detect as little as 1 µg protein equivalent
  • Fast—complete in less than 4-hours—no more overnight incubation
  • Flexible—compatible with all major exosome isolation methods (e.g. ExoQuick, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human samples
  • Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD81
  • Sample-saving—requires significantly less sample than our standard ExoELISA Kit, leaving more for other downstream applications
Choose the exosome quantitation method that’s best for your studies
 ExoELISA-ULTRA Complete KitsExoELISA KitsEXOCETFluoroCet
UseFor fast and sensitive antibody-based quantitation of exosomesFor sensitive quantitation of exosomes when time and input sample are not limitingFor fast quantitation of extracellular vesicles with moderate sample input requirementsFor the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection methodAntibodyAntibodyEnzymaticEnzymatic
Quantitation chemistryEnzymatic (HRP)Enzymatic (HRP)ColorimetricFluorescent
Total protocol time4 hours (no overnight incubation)24 hours20 min60 min
Input sample amount (protein equivalent)1 – 200 µg>500 µg50 µg<1 µg
Learn MoreExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA-ULTRA CD9
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCETFluoroCet

How It Works

Supporting Data

Supporting Data

The standard curve for ExoELISA-ULTRA CD81 shows robust linearity down to ~4 x 109 exosomes.

The standard curve for ExoELISA-ULTRA CD81 provides robust linearity down to ~4 x 10^9 exosomes.

Figure 1. ExoELISA-ULTRA CD81 standard curve shows robust linearity down to ~4 x 109 exosomes. OD450nm values of commonly used samples (e.g. human serum, HEK293T, and MCF7 breast cancer exosomes – in large colored dots) fall well within the standard curve for the assay.

Citations