TransDux™ (Original) Virus Transduction Reagent

Achieve high transduction efficiencies into most cell types, even those that are resistant to infection, with TransDux Transduction Reagent
  • High transduction efficiency
  • Broad cell type compatibility
  • Minimal hands-on time

Products

Catalog Number Description Size Price Quantity Add to Cart
LV850A-1 TransDux virus transduction reagent (200x) 100 Transductions $252
- +

Overview

Overview

Infect more cells with TransDux™

Increase your transduction efficiencies with TransDux (Original) Virus Transduction Reagent. Simply mix with virus to see 2-fold or greater (depending on cell type) transduction efficiencies over polybrene addition.

Or try our newer TransDux MAX™ Virus Transduction Reagent for an even greater increase in infectivity.

  • High transduction efficiency
  • Broad cell type compatibility
  • Minimal hands-on time

How It Works

How It Works

Get higher transduction efficiencies with minimal hands-on time

Simply combine culture medium with TransDux to a final concentration of 1x, add to cells, and then add virus. Swirl to mix, and proceed with the transduction as usual—72-hours post transduction, the viral genome will be integrated into the host cell genome.

Supporting Data

Supporting Data

Increase transduction efficiencies with TransDux Increase transduction efficiencies with TransDux

Figure 1. Our original TransDux Reagent increases transduction efficiencies at least 2-fold compared to polybrene. Transduction of either HepG2 cells (top panels) or HT1080 cells (bottom panels) with the addition of polybrene (left two panels) or TransDux (middle two panels). The right two panels show quantitative measures of infectivity using the Global UltraRapid Titer Kit. Both imaging and infectivity data show that TransDux increased the infectivity of lentivirus in both cell types as compared to polybrene.

Resources

Citations

  • Eisfeld, AK, et al. (2017) Mutations in the CCND1 and CCND2 genes are frequent events in adult patients with t(8;21)(q22;q22) acute myeloid leukemia.. Leukemia. 2017 Jun 1; 31(6):1278-1285. PM ID: 27843138
  • Swiercz, R, et al. (2017) Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption. Oncotarget. 2017 Jan 10; 8(2):3528-3541. PM ID: 27974681
  • Lin, ZY, et al. (2017) MicroRNA-30d promotes angiogenesis and tumor growth via MYPT1/c-JUN/VEGFA pathway and predicts aggressive outcome in prostate cancer. Mol. Cancer. 2017 Feb 27; 16(1):48. PM ID: 28241827
  • Zeng, Q, et al. (2017) Interleukin-37 suppresses the osteogenic responses of human aortic valve interstitial cells in vitro and alleviates valve lesions in mice. Proc. Natl. Acad. Sci. U.S.A.. 2017 Feb 14; 114(7):1631-1636. PM ID: 28137840
  • Takahashi, H, et al. (2017) Autophagy is required for cell survival under L-asparaginase-induced metabolic stress in acute lymphoblastic leukemia cells. Oncogene. 2017 Mar 27;. PM ID: 28346428
  • Song, R, et al. (2017) An epigenetic regulatory loop controls pro-osteogenic activation by TGF-β1 or bone morphogenetic protein 2 in human aortic valve interstitial cells. J. Biol. Chem.. 2017 May 26; 292(21):8657-8666. PM ID: 28377507
  • Mo, RJ, et al. (2017) Decreased HoxD10 expression promotes a proliferative and aggressive phenotype in prostate cancer. Curr. Mol. Med.. 2017 Feb 19;. PM ID: 28231752
  • Song, R, et al. (2017) Altered MicroRNA Expression Is Responsible for the Pro-Osteogenic Phenotype of Interstitial Cells in Calcified Human Aortic Valves. J Am Heart Assoc. 2017 Apr 24; 6(4). PM ID: 28438736
  • Li, P, et al. (2017) SIRT1 is required for mitochondrial biogenesis reprogramming in hypoxic human pulmonary arteriolar smooth muscle cells. Int. J. Mol. Med.. 2017 May 1; 39(5):1127-1136. PM ID: 28339017
  • Lopez, CM. (2017) MiR-34a Regulates the Invasive Capacity of Canine Osteosarcoma Cell Lines. Thesis. ;. Link: Thesis
  • Liang, YX, et al. (2016) E2F1 promotes tumor cell invasion and migration through regulating CD147 in prostate cancer. Int. J. Oncol.. 2016 Apr 1; 48(4):1650-8. PM ID: 26891801
  • Li, Q, et al. (2016) Development and Validation of a Rex1-RFP Potency Activity Reporter Assay That Quantifies Stress-Forced Potency Loss in Mouse Embryonic Stem Cells.. Stem Cells Dev.. 2016 Feb 15; 25(4):320-8. PM ID: 26651054
  • Li, Q, et al. (2016) High-Throughput Screens for Embryonic Stem Cells: Stress-Forced Potency-Stemness Loss Enables Toxicological Assays. Protocol. 2016 Oct 8;:1-34. Link: Protocol
  • Lesnik, J, et al. (2016) Registered report: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Elife. 2016 Jan 29; 5:e07383. PM ID: 26826285
  • Irvine, DA, et al. (2016) Deregulated hedgehog pathway signaling is inhibited by the smoothened antagonist LDE225 (Sonidegib) in chronic phase chronic myeloid leukaemia. Sci Rep. 2016 May 9; 6:25476. PM ID: 27157927
  • Chen, Y, et al. (2016) Calcineurin β protects brain after injury by activating the unfolded protein response. Neurobiol. Dis.. 2016 Oct 1; 94:139-56. PM ID: 27334877
  • Tilghman, J, et al. (2016) Regulation of Glioblastoma Tumor-Propagating Cells by the Integrin Partner Tetraspanin CD151. Neoplasia. 2016 Mar 1; 18(3):185-98. PM ID: 26992919
  • Westbroek, W, et al. (2016) A new glucocerebrosidase-deficient neuronal cell model provides a tool to probe pathophysiology and therapeutics for Gaucher disease. Dis Model Mech. 2016 Jul 1; 9(7):769-78. PM ID: 27482815
  • Shrivastava, S, et al. (2016) The Role of HMGB1 in Radioresistance of Bladder Cancer. Mol. Cancer Ther.. 2016 Mar 1; 15(3):471-9. PM ID: 26719575
  • Zhou, A, et al. (2016) RNA Binding Protein HuR Regulates Cardiac Sodium Channel Gene (SCN5A) Expression Through Transcriptional and Post-Transcriptional Pathways. Circulation. 2016 Nov 11; 134(1):A14386. Link: Circulation

Products

Catalog Number Description Size Price Quantity Add to Cart
LV850A-1 TransDux virus transduction reagent (200x) 100 Transductions $252
- +

Overview

Overview

Infect more cells with TransDux™

Increase your transduction efficiencies with TransDux (Original) Virus Transduction Reagent. Simply mix with virus to see 2-fold or greater (depending on cell type) transduction efficiencies over polybrene addition.

Or try our newer TransDux MAX™ Virus Transduction Reagent for an even greater increase in infectivity.

  • High transduction efficiency
  • Broad cell type compatibility
  • Minimal hands-on time

How It Works

How It Works

Get higher transduction efficiencies with minimal hands-on time

Simply combine culture medium with TransDux to a final concentration of 1x, add to cells, and then add virus. Swirl to mix, and proceed with the transduction as usual—72-hours post transduction, the viral genome will be integrated into the host cell genome.

Supporting Data

Supporting Data

Increase transduction efficiencies with TransDux Increase transduction efficiencies with TransDux

Figure 1. Our original TransDux Reagent increases transduction efficiencies at least 2-fold compared to polybrene. Transduction of either HepG2 cells (top panels) or HT1080 cells (bottom panels) with the addition of polybrene (left two panels) or TransDux (middle two panels). The right two panels show quantitative measures of infectivity using the Global UltraRapid Titer Kit. Both imaging and infectivity data show that TransDux increased the infectivity of lentivirus in both cell types as compared to polybrene.

Citations

  • Eisfeld, AK, et al. (2017) Mutations in the CCND1 and CCND2 genes are frequent events in adult patients with t(8;21)(q22;q22) acute myeloid leukemia.. Leukemia. 2017 Jun 1; 31(6):1278-1285. PM ID: 27843138
  • Swiercz, R, et al. (2017) Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption. Oncotarget. 2017 Jan 10; 8(2):3528-3541. PM ID: 27974681
  • Lin, ZY, et al. (2017) MicroRNA-30d promotes angiogenesis and tumor growth via MYPT1/c-JUN/VEGFA pathway and predicts aggressive outcome in prostate cancer. Mol. Cancer. 2017 Feb 27; 16(1):48. PM ID: 28241827
  • Zeng, Q, et al. (2017) Interleukin-37 suppresses the osteogenic responses of human aortic valve interstitial cells in vitro and alleviates valve lesions in mice. Proc. Natl. Acad. Sci. U.S.A.. 2017 Feb 14; 114(7):1631-1636. PM ID: 28137840
  • Takahashi, H, et al. (2017) Autophagy is required for cell survival under L-asparaginase-induced metabolic stress in acute lymphoblastic leukemia cells. Oncogene. 2017 Mar 27;. PM ID: 28346428
  • Song, R, et al. (2017) An epigenetic regulatory loop controls pro-osteogenic activation by TGF-β1 or bone morphogenetic protein 2 in human aortic valve interstitial cells. J. Biol. Chem.. 2017 May 26; 292(21):8657-8666. PM ID: 28377507
  • Mo, RJ, et al. (2017) Decreased HoxD10 expression promotes a proliferative and aggressive phenotype in prostate cancer. Curr. Mol. Med.. 2017 Feb 19;. PM ID: 28231752
  • Song, R, et al. (2017) Altered MicroRNA Expression Is Responsible for the Pro-Osteogenic Phenotype of Interstitial Cells in Calcified Human Aortic Valves. J Am Heart Assoc. 2017 Apr 24; 6(4). PM ID: 28438736
  • Li, P, et al. (2017) SIRT1 is required for mitochondrial biogenesis reprogramming in hypoxic human pulmonary arteriolar smooth muscle cells. Int. J. Mol. Med.. 2017 May 1; 39(5):1127-1136. PM ID: 28339017
  • Lopez, CM. (2017) MiR-34a Regulates the Invasive Capacity of Canine Osteosarcoma Cell Lines. Thesis. ;. Link: Thesis
  • Liang, YX, et al. (2016) E2F1 promotes tumor cell invasion and migration through regulating CD147 in prostate cancer. Int. J. Oncol.. 2016 Apr 1; 48(4):1650-8. PM ID: 26891801
  • Li, Q, et al. (2016) Development and Validation of a Rex1-RFP Potency Activity Reporter Assay That Quantifies Stress-Forced Potency Loss in Mouse Embryonic Stem Cells.. Stem Cells Dev.. 2016 Feb 15; 25(4):320-8. PM ID: 26651054
  • Li, Q, et al. (2016) High-Throughput Screens for Embryonic Stem Cells: Stress-Forced Potency-Stemness Loss Enables Toxicological Assays. Protocol. 2016 Oct 8;:1-34. Link: Protocol
  • Lesnik, J, et al. (2016) Registered report: Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Elife. 2016 Jan 29; 5:e07383. PM ID: 26826285
  • Irvine, DA, et al. (2016) Deregulated hedgehog pathway signaling is inhibited by the smoothened antagonist LDE225 (Sonidegib) in chronic phase chronic myeloid leukaemia. Sci Rep. 2016 May 9; 6:25476. PM ID: 27157927
  • Chen, Y, et al. (2016) Calcineurin β protects brain after injury by activating the unfolded protein response. Neurobiol. Dis.. 2016 Oct 1; 94:139-56. PM ID: 27334877
  • Tilghman, J, et al. (2016) Regulation of Glioblastoma Tumor-Propagating Cells by the Integrin Partner Tetraspanin CD151. Neoplasia. 2016 Mar 1; 18(3):185-98. PM ID: 26992919
  • Westbroek, W, et al. (2016) A new glucocerebrosidase-deficient neuronal cell model provides a tool to probe pathophysiology and therapeutics for Gaucher disease. Dis Model Mech. 2016 Jul 1; 9(7):769-78. PM ID: 27482815
  • Shrivastava, S, et al. (2016) The Role of HMGB1 in Radioresistance of Bladder Cancer. Mol. Cancer Ther.. 2016 Mar 1; 15(3):471-9. PM ID: 26719575
  • Zhou, A, et al. (2016) RNA Binding Protein HuR Regulates Cardiac Sodium Channel Gene (SCN5A) Expression Through Transcriptional and Post-Transcriptional Pathways. Circulation. 2016 Nov 11; 134(1):A14386. Link: Circulation