PureFection™ Transfection Reagent

Deliver more nucleic acids—plasmids, siRNAs, etc.—to cells than the leading lipid-based transfection reagent for effective and reproducible transfection
  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

Products

Catalog Number Description Size Price Quantity Add to Cart
LV750A-1 PureFection Transfection Reagent 1 mL $297
- +
LV750A-5 PureFection Transfection Reagent 5 mL $1262
- +

Overview

Overview

Increase your transfection efficiencies

With SBI’s PureFection™ Transfection Reagent, you can deliver more nucleic acid—plasmids, siRNAs, etc.—than the leading lipid-based transfection reagent for effective, efficient, and reproducible transfections.

The easy-to-use protocol consists of a rapid, one-step, 15-minute incubation with the plasmid, small RNA, or other nucleic acid you’d like to transfect. Once the incubation is done, simply add directly to target cells—no media changes are required as PureFection works in the presence of antibiotics and serum.

The fast PureFection protocol makes it well-suited for high-throughput transfection projects.

  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

How It Works

How It Works

A fast and effective method for increasing transfection efficiencies

The PureFection Transfection Reagent uses a fast, 15-minute protocol

Supporting Data

Supporting Data

PureFection delivers higher transfection efficiencies than the leading lipid-based reagent

PureFection delivers higher transfection efficiencies than the leading lipid-based reagentPureFection delivers higher transfection efficiencies than the leading lipid-based reagent

Resources

Citations

  • Mai, A, et al. (2022) Thymoquinone induces apoptosis in temozolomide-resistant glioblastoma cells via the p38 mitogen-activated protein kinase signaling pathway. Environmental toxicology. 1970 Jan 1;. PM ID: 36176197
  • Le, Q, et al. (2022) Reversion of glucocorticoid-induced senescence and collagen synthesis decrease by LY294002 is mediated through p38 in skin. International Journal of Biological Sciences. 1970 Jan 1; 18(16):6102-6113. Link: International Journal of Biological Sciences
  • Zhang, WC, et al. (2022) MicroRNA-21 guide and passenger strand regulation of adenylosuccinate lyase-mediated purine metabolism promotes transition to an EGFR-TKI-tolerant persister state. Cancer gene therapy. 1970 Jan 1;. PM ID: 35840668
  • Stofner, S & Konig, EM. (2022) The role of serine/threonine kinases STK38 and STK38L in human natural killer cells. Thesis. 1970 Jan 1;. Link: Thesis
  • Savill, KMZ, et al. (2022) Distinct resistance mechanisms arise to allosteric vs. ATP-competitive AKT inhibitors. Nature communications. 1970 Jan 1; 13(1):2057. PM ID: 35440108
  • Yogeswaran, A, et al. (2021) The C0-C1f Region of Cardiac Myosin Binding Protein-C Induces Pro-Inflammatory Responses in Fibroblasts via TLR4 Signaling. Cells. 1970 Jan 1; 10(6). PM ID: 34073556
  • Nakayama, Y, et al. (2021) Txnip C247S mutation protects the heart against acute myocardial infarction. Journal of molecular and cellular cardiology. 1970 Jan 1;. PM ID: 33652022
  • Liang, J, et al. (2021) AKR1C3 and Its Transcription Factor HOXB4 Are Promising Diagnostic Biomarkers for Acute Myocardial Infarction. Frontiers in cardiovascular medicine. 1970 Jan 1; 8:694238. PM ID: 34568444
  • Lin, KH, et al. (2021) Diallyl Trisulfide Suppresses High-Glucose-Induced Cardiomyocyte Apoptosis by Targeting Reactive Oxygen Species-Mediated Hypoxia-Inducible Factor-1α/Insulin-like Growth Factor Binding Protein 3 Activation. Journal of agricultural and food chemistry. 1970 Jan 1;. PM ID: 34558885
  • Chuang, TD, et al. (2021) Long Noncoding RNA MIAT Modulates the Extracellular Matrix Deposition in Leiomyomas by Sponging MiR-29 Family. Endocrinology. 1970 Jan 1; 162(11). PM ID: 34491311
  • Lin, KH, et al. (2021) MicroRNA-210 repression facilitates advanced glycation end-product (AGE)-induced cardiac mitochondrial dysfunction and apoptosis via JNK activation. Journal of cellular biochemistry. 1970 Jan 1;. PM ID: 34545968
  • Lin, KH, et al. (2021) Carboxyl terminus of HSP70-interacting protein attenuates advanced glycation end products-induced cardiac injuries by promoting NFκB proteasomal degradation. Journal of cellular physiology. 1970 Jan 1;. PM ID: 34958118
  • Ho, CC, et al. (2020) Extracts of Jasminum sambac flowers fermented by Lactobacillus rhamnosus inhibit H2 O2 – and UVB-induced aging in human dermal fibroblasts. Environmental toxicology. 1970 Jan 1;:e23065. PM ID: 33270331
  • Chuang, TD, Rehan, A & Khorram, O. (2020) Functional role of the long noncoding RNA X-inactive specific transcript in leiomyoma pathogenesis. Fertil Steril. 1970 Jan 1;. PM ID: 33070965
  • Chuang, TD, et al. (2020) Mechanism underlying increased cardiac extracellular matrix deposition in perinatal nicotine-exposed offspring. Am. J. Physiol. Heart Circ. Physiol.. 1970 Jan 1; 319(3):H651-H660. PM ID: 32795172
  • Hsieh, DJ, et al. (2020) Diallyl Trisulfide (DATS) Suppresses AGE-Induced Cardiomyocyte Apoptosis by Targeting ROS-Mediated PKCδ Activation. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32283691
  • Chuang, TD, Rehan, A & Khorram, O. (2020) Tranilast induces MiR-200c expression through blockade of RelA/p65 activity in leiomyoma smooth muscle cells. Fertil. Steril.. 1970 Jan 1;. PM ID: 32199621
  • Liu, SP, et al. (2020) Tetramethylpyrazine reverses high-glucose induced hypoxic effects by negatively regulating HIF-1α induced BNIP3 expression to ameliorate H9c2 cardiomyoblast apoptosis. Nutr Metab (Lond). 1970 Jan 1; 17:12. PM ID: 32021640
  • Wen, SY, et al. (2019) Roles of p38α and p38β mitogen‑activated protein kinase isoforms in human malignant melanoma A375 cells. International journal of molecular medicine. 1970 Jan 1; 44(6):2123-2132. PM ID: 31661126
  • Chuang, TD & Khorram, O. (2019) Cross-talk between miR-29c and transforming growth factor-β3 is mediated by an epigenetic mechanism in leiomyoma. Fertility and sterility. 1970 Jan 1; 112(6):1180-1189. PM ID: 31843095

Products

Catalog Number Description Size Price Quantity Add to Cart
LV750A-1 PureFection Transfection Reagent 1 mL $297
- +
LV750A-5 PureFection Transfection Reagent 5 mL $1262
- +

Overview

Overview

Increase your transfection efficiencies

With SBI’s PureFection™ Transfection Reagent, you can deliver more nucleic acid—plasmids, siRNAs, etc.—than the leading lipid-based transfection reagent for effective, efficient, and reproducible transfections.

The easy-to-use protocol consists of a rapid, one-step, 15-minute incubation with the plasmid, small RNA, or other nucleic acid you’d like to transfect. Once the incubation is done, simply add directly to target cells—no media changes are required as PureFection works in the presence of antibiotics and serum.

The fast PureFection protocol makes it well-suited for high-throughput transfection projects.

  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

How It Works

How It Works

A fast and effective method for increasing transfection efficiencies

The PureFection Transfection Reagent uses a fast, 15-minute protocol

Supporting Data

Supporting Data

PureFection delivers higher transfection efficiencies than the leading lipid-based reagent

PureFection delivers higher transfection efficiencies than the leading lipid-based reagentPureFection delivers higher transfection efficiencies than the leading lipid-based reagent

Citations

  • Mai, A, et al. (2022) Thymoquinone induces apoptosis in temozolomide-resistant glioblastoma cells via the p38 mitogen-activated protein kinase signaling pathway. Environmental toxicology. 1970 Jan 1;. PM ID: 36176197
  • Le, Q, et al. (2022) Reversion of glucocorticoid-induced senescence and collagen synthesis decrease by LY294002 is mediated through p38 in skin. International Journal of Biological Sciences. 1970 Jan 1; 18(16):6102-6113. Link: International Journal of Biological Sciences
  • Zhang, WC, et al. (2022) MicroRNA-21 guide and passenger strand regulation of adenylosuccinate lyase-mediated purine metabolism promotes transition to an EGFR-TKI-tolerant persister state. Cancer gene therapy. 1970 Jan 1;. PM ID: 35840668
  • Stofner, S & Konig, EM. (2022) The role of serine/threonine kinases STK38 and STK38L in human natural killer cells. Thesis. 1970 Jan 1;. Link: Thesis
  • Savill, KMZ, et al. (2022) Distinct resistance mechanisms arise to allosteric vs. ATP-competitive AKT inhibitors. Nature communications. 1970 Jan 1; 13(1):2057. PM ID: 35440108
  • Yogeswaran, A, et al. (2021) The C0-C1f Region of Cardiac Myosin Binding Protein-C Induces Pro-Inflammatory Responses in Fibroblasts via TLR4 Signaling. Cells. 1970 Jan 1; 10(6). PM ID: 34073556
  • Nakayama, Y, et al. (2021) Txnip C247S mutation protects the heart against acute myocardial infarction. Journal of molecular and cellular cardiology. 1970 Jan 1;. PM ID: 33652022
  • Liang, J, et al. (2021) AKR1C3 and Its Transcription Factor HOXB4 Are Promising Diagnostic Biomarkers for Acute Myocardial Infarction. Frontiers in cardiovascular medicine. 1970 Jan 1; 8:694238. PM ID: 34568444
  • Lin, KH, et al. (2021) Diallyl Trisulfide Suppresses High-Glucose-Induced Cardiomyocyte Apoptosis by Targeting Reactive Oxygen Species-Mediated Hypoxia-Inducible Factor-1α/Insulin-like Growth Factor Binding Protein 3 Activation. Journal of agricultural and food chemistry. 1970 Jan 1;. PM ID: 34558885
  • Chuang, TD, et al. (2021) Long Noncoding RNA MIAT Modulates the Extracellular Matrix Deposition in Leiomyomas by Sponging MiR-29 Family. Endocrinology. 1970 Jan 1; 162(11). PM ID: 34491311
  • Lin, KH, et al. (2021) MicroRNA-210 repression facilitates advanced glycation end-product (AGE)-induced cardiac mitochondrial dysfunction and apoptosis via JNK activation. Journal of cellular biochemistry. 1970 Jan 1;. PM ID: 34545968
  • Lin, KH, et al. (2021) Carboxyl terminus of HSP70-interacting protein attenuates advanced glycation end products-induced cardiac injuries by promoting NFκB proteasomal degradation. Journal of cellular physiology. 1970 Jan 1;. PM ID: 34958118
  • Ho, CC, et al. (2020) Extracts of Jasminum sambac flowers fermented by Lactobacillus rhamnosus inhibit H2 O2 – and UVB-induced aging in human dermal fibroblasts. Environmental toxicology. 1970 Jan 1;:e23065. PM ID: 33270331
  • Chuang, TD, Rehan, A & Khorram, O. (2020) Functional role of the long noncoding RNA X-inactive specific transcript in leiomyoma pathogenesis. Fertil Steril. 1970 Jan 1;. PM ID: 33070965
  • Chuang, TD, et al. (2020) Mechanism underlying increased cardiac extracellular matrix deposition in perinatal nicotine-exposed offspring. Am. J. Physiol. Heart Circ. Physiol.. 1970 Jan 1; 319(3):H651-H660. PM ID: 32795172
  • Hsieh, DJ, et al. (2020) Diallyl Trisulfide (DATS) Suppresses AGE-Induced Cardiomyocyte Apoptosis by Targeting ROS-Mediated PKCδ Activation. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32283691
  • Chuang, TD, Rehan, A & Khorram, O. (2020) Tranilast induces MiR-200c expression through blockade of RelA/p65 activity in leiomyoma smooth muscle cells. Fertil. Steril.. 1970 Jan 1;. PM ID: 32199621
  • Liu, SP, et al. (2020) Tetramethylpyrazine reverses high-glucose induced hypoxic effects by negatively regulating HIF-1α induced BNIP3 expression to ameliorate H9c2 cardiomyoblast apoptosis. Nutr Metab (Lond). 1970 Jan 1; 17:12. PM ID: 32021640
  • Wen, SY, et al. (2019) Roles of p38α and p38β mitogen‑activated protein kinase isoforms in human malignant melanoma A375 cells. International journal of molecular medicine. 1970 Jan 1; 44(6):2123-2132. PM ID: 31661126
  • Chuang, TD & Khorram, O. (2019) Cross-talk between miR-29c and transforming growth factor-β3 is mediated by an epigenetic mechanism in leiomyoma. Fertility and sterility. 1970 Jan 1; 112(6):1180-1189. PM ID: 31843095