Tetraspanin Exo-Flow Capture Kit

For the selective capture and FACS of exosome subpopulations based on the presence of three general exosome markers, the tetraspanins CD9, CD63, and CD81
  • A range of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Reversible Exo-FITC and Exo-APC stains can be completely removed after FACS
  • Exosome Elution Buffer simultaneously removes Exo-FITC (or Exo-APC) and elutes exosomes from the magnetic beads for downstream applications such as functional studies

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOFLOW150A-1 Tetraspanin Exo-Flow Combo capture kit (Magnetic streptavidin beads, CD9, CD63, and CD81-biotin capture antibodies, Wash and Elution Buffers, Exo-FITC stain) 10 Reactions $729.00
- +
Contact Us

Overview

Overview

Go with the flow for easy exosome isolation using FACS The Tetraspanin Exo-Flow Capture Kit has all the reagents you need to purify exosomes using FACS—magnetic streptavidin beads, CD9-, CD63- and CD81-biotin capture antibodies, wash and elution buffers, and reversible Exo-FITC stain (Cat.# EXOFLOW800A-1)—you supply the FACS and the optional magnetic stand (Cat.# EXOFLOW700A-1). Our well-validated tetraspanin-biotin antibodies—CD9, CD63, and CD81—and high-quality kit components ensure reliable, reproducible exosome purification based on the presence of three general exosome markers (CD9, CD63, and CD81). And with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations. Note that we also offer reversible Exo-APC (Cat.# Exo-FLOW810A-1) as an alternative stain to Exo-FITC.
  • A range of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Reversible Exo-FITC and Exo-APC stains can be completely removed after FACS
  • Exosome Elution Buffer simultaneously removes Exo-FITC (or Exo-APC) and elutes exosomes from the magnetic beads for downstream applications such as functional studies
Exosome capture is highly efficient with large bead sizeMore than just Tetraspanins To facilitate the widest range of studies, SBI built the Exo-Flow system to be highly modular. We offer a range of biotinylated antibodies as well as a Basic Exo-Flow Kit (Cat.# CSFLOWBASICA-1) with no antibody so that you can use your own biotinylated antibodies.
Cat.#Kit
EXOFLOW100A-1CD9 Exo-Flow Capture Kit
EXOFLOW150A-1Tetraspanin Exo-Flow Combo Capture Kit
EXOFLOW200A-1CD31 Exo-Flow Capture Kit
EXOFLOW210A-1CD44 Exo-Flow Capture Kit
EXOFLOW300A-1CD63 Exo-Flow Capture Kit
EXOFLOW400A-1CD81 Exo-Flow Capture Kit
EXOFLOW500A-1Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1CD14 Exo-Flow Capture Kit
EXOFLOW620A-1CD68 Exo-Flow Capture Kit
EXOFLOW630A-1EpCAM Exo-Flow Capture Kit
EXOFLOW660A-1CD73 Exo-Flow Capture Kit

How It Works

How It Works

Easily purify exosomes using FACS with the Tetraspanin Exo-Flow Capture Kit

At a glance
Simply (1) couple the tetraspanin-biotin antibodies to the magnetic streptavidin beads, (2) use the tetraspanin-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).

Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.

How the Exo-Flow Capture Kit’s magnetic stand works

Supporting Data

Supporting Data

See the Exo-FLOW Capture Kit data for CD9, CD63, and CD81 markers

Highly selective exosome isolation by FACS using antibodies to the tetraspaninsHighly selective exosome isolation by FACS using antibodies to the tetraspanins

The Tetraspanin Exo-Flow Capture Kit delivers quantitative, highly selective exosome isolation. Bead flow separation data for exosomes secreted by HEK293 cells and captured using either the CD9 (top row), CD63 (middle row), or CD81 (bottom row) Exo-Flow Capture Kits. Plots of forward scatter versus FITC intensity show that in the no exosome control, only 0.5% of particles are FITC-positive (left panels), whereas in the exosome-containing sample, 99.3% of particles are FITC-positive (middle panels). The highly linear binding capacity of the Exo-Flow beads is shown (right panel, CD9), with the degree of flow separation shown in the inset graph for CD9, and in the right panels for CD63 and CD81.
Human serum exosomes were isolated from 250 µL serum using ExoQuick® and the exosome pellet resuspended in 500 µL of 1x PBS. Exosome particles were added as two-fold dilutions starting at 50 µL, and then captured using the biotinylated antibody coupled to Exo-Flow beads. The FITC flow cytometric intensities are then plotted versus the number of exosome particles (determined using NanoSight).

Resources

Citations

  • Sheller-Miller, S, et al. (2019) Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Sci Rep. 2019 Jan 24; 9(1):608. PM ID: 30679631
  • Sheller-Miller, S, et al. (2019) Cre-Reporter Mouse Model to Determine Exosome Communication and Function during Pregnancy. Am. J. Obstet. Gynecol.. 2019 Jun 14;. PM ID: 31207235
  • Genschmer, KR, et al. (2019) Activated PMN Exosomes: Pathogenic Entities Causing Matrix Destruction and Disease in the Lung. Cell. 2019 Jan 10; 176(1-2):113-126.e15. PM ID: 30633902
  • Chen, C, et al. (2017) Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis. Cell Res.. 2017 Apr 1; 27(4):559-577. PM ID: 28106077
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product
  • Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm.. 2017 Jun 21; 2017:4814987. PM ID: 28634420
  • Meyer, C, et al. (2017) Pseudotyping exosomes for enhanced protein delivery in mammalian cells.. Int J Nanomedicine. 2017 May 1; 12:3153-3170. PM ID: 28458537
  • Etzerodt, A, et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma. Sci Rep. 2017 Jan 13; 7:40286. PM ID: 28084321
  • Raimondo, F, et al. (2016) Urinary proteomics for the study of genetic kidney diseases.. Expert Rev Proteomics. 2016 Mar 2; 13(3):309-24. PM ID: 26698090
  • Smith, JA & Daniel, R. (2016) Human vaginal fluid contains exosomes that have an inhibitory effect on an early step of the HIV-1 life cycle. AIDS. 2016 Nov 13; 30(17):2611-2616. PM ID: 27536982
  • Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry. J Cancer Prev. 2016 Sep 1; 21(3):194-200. PM ID: 27722146
  • Junker, K, et al. (2016) Extracellular Vesicles and Their Role in Urologic Malignancies.. Eur. Urol.. 2016 Aug 1; 70(2):323-31. PM ID: 26924769
  • Wen, Z, et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs. J. Clin. Invest.. 2016 May 2; 126(5):1953-67. PM ID: 27088800
  • Nudel, K. (2016) Neisseria gonorrhoeae modulates epithelial cell responses via the induction and release of the inhibitor of apoptosis protein cIAP2 in exosomes. Thesis. ;. Link: Thesis
  • Mukherjee, K, et al. (2016) Reversible HuR-microRNA binding controls extracellular export of miR-122 and augments stress response. EMBO Rep.. 2016 Aug 1; 17(8):1184-203. PM ID: 27402548
  • Peterson, MF, et al. (2015) Integrated systems for exosome investigation. Methods. 2015 Oct 1; 87:31-45. PM ID: 25916618
  • Bhattarai, N, et al. (2015) Conserved Motifs within Hepatitis C Virus Envelope (E2) RNA and Protein Independently Inhibit T Cell Activation. PLoS Pathog.. 2015 Sep 1; 11(9):e1005183. PM ID: 26421924
  • Bednarczyk, M, Biryło, M & DAWIDOWICZ, A. (2015) Modern Geodetic Techniques in Spatial Measurement. Thesis. ;. Link: Thesis
  • Nudel, K, Massari, P & Genco, CA. (2015) Neisseria gonorrhoeae Modulates Cell Death in Human Endocervical Epithelial Cells through Export of Exosome-Associated cIAP2. Infect. Immun.. 2015 Sep 1; 83(9):3410-7. PM ID: 26077759
  • Jia, S, et al. (2014) Emerging technologies in extracellular vesicle-based molecular diagnostics. Expert Rev. Mol. Diagn.. 2014 Apr 1; 14(3):307-21. PM ID: 24575799

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOFLOW150A-1 Tetraspanin Exo-Flow Combo capture kit (Magnetic streptavidin beads, CD9, CD63, and CD81-biotin capture antibodies, Wash and Elution Buffers, Exo-FITC stain) 10 Reactions $729.00
- +
Contact Us

Overview

Overview

Go with the flow for easy exosome isolation using FACS The Tetraspanin Exo-Flow Capture Kit has all the reagents you need to purify exosomes using FACS—magnetic streptavidin beads, CD9-, CD63- and CD81-biotin capture antibodies, wash and elution buffers, and reversible Exo-FITC stain (Cat.# EXOFLOW800A-1)—you supply the FACS and the optional magnetic stand (Cat.# EXOFLOW700A-1). Our well-validated tetraspanin-biotin antibodies—CD9, CD63, and CD81—and high-quality kit components ensure reliable, reproducible exosome purification based on the presence of three general exosome markers (CD9, CD63, and CD81). And with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations. Note that we also offer reversible Exo-APC (Cat.# Exo-FLOW810A-1) as an alternative stain to Exo-FITC.
  • A range of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Reversible Exo-FITC and Exo-APC stains can be completely removed after FACS
  • Exosome Elution Buffer simultaneously removes Exo-FITC (or Exo-APC) and elutes exosomes from the magnetic beads for downstream applications such as functional studies
Exosome capture is highly efficient with large bead sizeMore than just Tetraspanins To facilitate the widest range of studies, SBI built the Exo-Flow system to be highly modular. We offer a range of biotinylated antibodies as well as a Basic Exo-Flow Kit (Cat.# CSFLOWBASICA-1) with no antibody so that you can use your own biotinylated antibodies.
Cat.#Kit
EXOFLOW100A-1CD9 Exo-Flow Capture Kit
EXOFLOW150A-1Tetraspanin Exo-Flow Combo Capture Kit
EXOFLOW200A-1CD31 Exo-Flow Capture Kit
EXOFLOW210A-1CD44 Exo-Flow Capture Kit
EXOFLOW300A-1CD63 Exo-Flow Capture Kit
EXOFLOW400A-1CD81 Exo-Flow Capture Kit
EXOFLOW500A-1Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1CD14 Exo-Flow Capture Kit
EXOFLOW620A-1CD68 Exo-Flow Capture Kit
EXOFLOW630A-1EpCAM Exo-Flow Capture Kit
EXOFLOW660A-1CD73 Exo-Flow Capture Kit

How It Works

How It Works

Easily purify exosomes using FACS with the Tetraspanin Exo-Flow Capture Kit

At a glance
Simply (1) couple the tetraspanin-biotin antibodies to the magnetic streptavidin beads, (2) use the tetraspanin-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).

Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.

How the Exo-Flow Capture Kit’s magnetic stand works

Supporting Data

Supporting Data

See the Exo-FLOW Capture Kit data for CD9, CD63, and CD81 markers

Highly selective exosome isolation by FACS using antibodies to the tetraspaninsHighly selective exosome isolation by FACS using antibodies to the tetraspanins

The Tetraspanin Exo-Flow Capture Kit delivers quantitative, highly selective exosome isolation. Bead flow separation data for exosomes secreted by HEK293 cells and captured using either the CD9 (top row), CD63 (middle row), or CD81 (bottom row) Exo-Flow Capture Kits. Plots of forward scatter versus FITC intensity show that in the no exosome control, only 0.5% of particles are FITC-positive (left panels), whereas in the exosome-containing sample, 99.3% of particles are FITC-positive (middle panels). The highly linear binding capacity of the Exo-Flow beads is shown (right panel, CD9), with the degree of flow separation shown in the inset graph for CD9, and in the right panels for CD63 and CD81.
Human serum exosomes were isolated from 250 µL serum using ExoQuick® and the exosome pellet resuspended in 500 µL of 1x PBS. Exosome particles were added as two-fold dilutions starting at 50 µL, and then captured using the biotinylated antibody coupled to Exo-Flow beads. The FITC flow cytometric intensities are then plotted versus the number of exosome particles (determined using NanoSight).

Citations

  • Sheller-Miller, S, et al. (2019) Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Sci Rep. 2019 Jan 24; 9(1):608. PM ID: 30679631
  • Sheller-Miller, S, et al. (2019) Cre-Reporter Mouse Model to Determine Exosome Communication and Function during Pregnancy. Am. J. Obstet. Gynecol.. 2019 Jun 14;. PM ID: 31207235
  • Genschmer, KR, et al. (2019) Activated PMN Exosomes: Pathogenic Entities Causing Matrix Destruction and Disease in the Lung. Cell. 2019 Jan 10; 176(1-2):113-126.e15. PM ID: 30633902
  • Chen, C, et al. (2017) Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis. Cell Res.. 2017 Apr 1; 27(4):559-577. PM ID: 28106077
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product
  • Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm.. 2017 Jun 21; 2017:4814987. PM ID: 28634420
  • Meyer, C, et al. (2017) Pseudotyping exosomes for enhanced protein delivery in mammalian cells.. Int J Nanomedicine. 2017 May 1; 12:3153-3170. PM ID: 28458537
  • Etzerodt, A, et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma. Sci Rep. 2017 Jan 13; 7:40286. PM ID: 28084321
  • Raimondo, F, et al. (2016) Urinary proteomics for the study of genetic kidney diseases.. Expert Rev Proteomics. 2016 Mar 2; 13(3):309-24. PM ID: 26698090
  • Smith, JA & Daniel, R. (2016) Human vaginal fluid contains exosomes that have an inhibitory effect on an early step of the HIV-1 life cycle. AIDS. 2016 Nov 13; 30(17):2611-2616. PM ID: 27536982
  • Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry. J Cancer Prev. 2016 Sep 1; 21(3):194-200. PM ID: 27722146
  • Junker, K, et al. (2016) Extracellular Vesicles and Their Role in Urologic Malignancies.. Eur. Urol.. 2016 Aug 1; 70(2):323-31. PM ID: 26924769
  • Wen, Z, et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs. J. Clin. Invest.. 2016 May 2; 126(5):1953-67. PM ID: 27088800
  • Nudel, K. (2016) Neisseria gonorrhoeae modulates epithelial cell responses via the induction and release of the inhibitor of apoptosis protein cIAP2 in exosomes. Thesis. ;. Link: Thesis
  • Mukherjee, K, et al. (2016) Reversible HuR-microRNA binding controls extracellular export of miR-122 and augments stress response. EMBO Rep.. 2016 Aug 1; 17(8):1184-203. PM ID: 27402548
  • Peterson, MF, et al. (2015) Integrated systems for exosome investigation. Methods. 2015 Oct 1; 87:31-45. PM ID: 25916618
  • Bhattarai, N, et al. (2015) Conserved Motifs within Hepatitis C Virus Envelope (E2) RNA and Protein Independently Inhibit T Cell Activation. PLoS Pathog.. 2015 Sep 1; 11(9):e1005183. PM ID: 26421924
  • Bednarczyk, M, Biryło, M & DAWIDOWICZ, A. (2015) Modern Geodetic Techniques in Spatial Measurement. Thesis. ;. Link: Thesis
  • Nudel, K, Massari, P & Genco, CA. (2015) Neisseria gonorrhoeae Modulates Cell Death in Human Endocervical Epithelial Cells through Export of Exosome-Associated cIAP2. Infect. Immun.. 2015 Sep 1; 83(9):3410-7. PM ID: 26077759
  • Jia, S, et al. (2014) Emerging technologies in extracellular vesicle-based molecular diagnostics. Expert Rev. Mol. Diagn.. 2014 Apr 1; 14(3):307-21. PM ID: 24575799