Rat Complete SeraMir Exosome RNA Amplification & Profiling Kit for Media & Urine

Get a quick start on exoRNA profiling with everything you need to extract exoRNAs from most biofluids and test expression against 380 rat miRs.
  • Reliable, reproducible exoRNA isolation from most biofluids
  • Sensitive detection via exoRNA amplification
  • Everything you need including 384-well plate of rat miRNAs for qPCR profiling

Products

Catalog Number Description Size Price Quantity Add to Cart
RA822TC-1 Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50 mL ExoQuick-TC, RA805A-1, RA812A-1 components) 20 Profiles $1503
- +

Overview

Overview

Get your exosomal RNA profiling studies off the ground fast With everything you need for profiling exosomal RNAs from most biofluids, SBI’s Rat Complete SeraMir Exosome RNA Amplification & Profiling Kit is a great way to get started with exoRNA profiling. The kit includes the fast and efficient ExoQuick-TC Exosome Isolation Reagent, phenol-free SeraMir exosome RNA purification reagents and columns, reagents for cDNA synthesis and amplification, and one 384-well plate with rat miRNAs and control wells for quantitation of isolated exoRNAs. (NOTE: for exoRNA isolation from serum, plasma, or ascites fluid, try the Rat Complete SeraMir Kit).
  • Reliable, reproducible exoRNA isolation from most biofluids
  • Sensitive detection via exoRNA amplification
  • Everything you need including 384-well plate of rat miRNAs for qPCR profiling
To find out which miRNAs are included, click here. Find exosomal RNA biomarkers using the SeraMir Exosome RNA Amplification Kit
Cat. #NameKit includes
ExoQuick or ExoQuick-TCSeraMir RNA columns and reagentsSeraMir cDNA synthesis and amplification reagents384-well plate miRNAs for human, mouse, or rat
RA800A-1Complete SeraMir Exosome RNA Amplification Kit 
RA800TC-1Complete SeraMir Exosome RNA Amplification Kit for Media and Urine 
RA806A-1SeraMir Exosome RNA Purification Kit  
RA806TC-1SeraMir Exosome RNA Purification Kit for Media and Urine  
RA808A-1SeraMir Exosome RNA Purification Column Kit   
RA820A-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA820TC-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA821A-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA821TC-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA822A-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA822TC-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine

How It Works

How It Works

Quickly and easily profile exoRNAs
  • Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
  • Purify exoRNAs with SeraMir columns
  • Measure expression levels of 380 rat miRNAs in a 384-well qPCR assay
Isolate serum exosomes and purify exoRNAs Streamline biomarker discovery with seramir – the workflow, steps 1 and 2 Tail exoRNAs and synthesize double-tagged cDNAStreamline biomarker discovery with seramir – the workflow, steps 3 and 4 Three validated reference controls included on the SeraMir Profiling qPCR plateRat miRNA profiling qPCR plate includes three validated reference controls Excellent technical replicates across an 8-log rangeExcellent technical replicates across 8 logs

Supporting Data

Supporting Data

Better qPCR profiling with SeraMir SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.

 SeraMir provides reliably better qPCR profiling than exosomal RNAs isolated using phenol/trizol

Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set. Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.

Resources

Citations

  • Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. ;. Link: Conference
  • Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. ;. Link: Abstract
  • Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017 Apr 15;. PM ID: 28411393
  • Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017 Jan 1; 25(1):102-110. PM ID: 27883272
  • Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017 Apr 1; 31(9):1277-1295. PM ID: 28447547
  • Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017 Feb 1; 15:163-172. PM ID: 27974246
  • Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017 Jun 13;. PM ID: 28623135
  • Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017 Jun 4;. PM ID: 28580875
  • Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017 Jun 1;:95-99. PM ID: 28643924
  • Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017 May 1; 4(2):112-121. PM ID: 28451447
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product
  • Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. ; 7(4). Link: bio-protocol
  • Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017 Jan 1; 21(1):55-66. PM ID: 28271982
  • Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017 Jun 27; 8(26):42712-42727. PM ID: 28514744
  • Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017 Apr 5;. PM ID: 28157838
  • Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016 Feb 1; 429:39-49. PM ID: 26723490
  • Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. ; 5(1):1–94. Link: Liver Cancer
  • Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016 Mar 1; 5:30924. PM ID: 26928673
  • Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016 Mar 2; 11(3):e0150154. PM ID: 26930277
  • Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016 Jul 20; 39:427-432. Link: 中华检验医学杂志
Rat Complete SeraMir Exosome RNA Amplification & Profiling Kit for Media & Urine $1,503.00

Products

Catalog Number Description Size Price Quantity Add to Cart
RA822TC-1 Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine (contains cat# RA800TC-1 with 50 mL ExoQuick-TC, RA805A-1, RA812A-1 components) 20 Profiles $1503
- +

Overview

Overview

Get your exosomal RNA profiling studies off the ground fast With everything you need for profiling exosomal RNAs from most biofluids, SBI’s Rat Complete SeraMir Exosome RNA Amplification & Profiling Kit is a great way to get started with exoRNA profiling. The kit includes the fast and efficient ExoQuick-TC Exosome Isolation Reagent, phenol-free SeraMir exosome RNA purification reagents and columns, reagents for cDNA synthesis and amplification, and one 384-well plate with rat miRNAs and control wells for quantitation of isolated exoRNAs. (NOTE: for exoRNA isolation from serum, plasma, or ascites fluid, try the Rat Complete SeraMir Kit).
  • Reliable, reproducible exoRNA isolation from most biofluids
  • Sensitive detection via exoRNA amplification
  • Everything you need including 384-well plate of rat miRNAs for qPCR profiling
To find out which miRNAs are included, click here. Find exosomal RNA biomarkers using the SeraMir Exosome RNA Amplification Kit
Cat. #NameKit includes
ExoQuick or ExoQuick-TCSeraMir RNA columns and reagentsSeraMir cDNA synthesis and amplification reagents384-well plate miRNAs for human, mouse, or rat
RA800A-1Complete SeraMir Exosome RNA Amplification Kit 
RA800TC-1Complete SeraMir Exosome RNA Amplification Kit for Media and Urine 
RA806A-1SeraMir Exosome RNA Purification Kit  
RA806TC-1SeraMir Exosome RNA Purification Kit for Media and Urine  
RA808A-1SeraMir Exosome RNA Purification Column Kit   
RA820A-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA820TC-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA821A-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA821TC-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA822A-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA822TC-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine

How It Works

How It Works

Quickly and easily profile exoRNAs
  • Isolate exosomes from patient biofluids with the included ExoQuick-TC reagent
  • Purify exoRNAs with SeraMir columns
  • Measure expression levels of 380 rat miRNAs in a 384-well qPCR assay
Isolate serum exosomes and purify exoRNAs Streamline biomarker discovery with seramir – the workflow, steps 1 and 2 Tail exoRNAs and synthesize double-tagged cDNAStreamline biomarker discovery with seramir – the workflow, steps 3 and 4 Three validated reference controls included on the SeraMir Profiling qPCR plateRat miRNA profiling qPCR plate includes three validated reference controls Excellent technical replicates across an 8-log rangeExcellent technical replicates across 8 logs

Supporting Data

Supporting Data

Better qPCR profiling with SeraMir SeraMir provides reliably better qPCR profiling than exosmal RNAs isolated using phenol/trizol

Figure 1. Serum RNA prepared by the SeraMir Kit delivers more reliable, reproducible qPCR profiles than when the RNA is isolated using conventional Trizol methods. Profiling of 380 human microRNAs across the SeraMir 384 Profiler. The phenol-free exosome lysis step coupled to the small RNA binding columns isolates exoRNAs with much higher purity than Trizol/Phenol based methods. The SeraMir exoRNAs are compatible with downstream polyadenylation and reverse trancription reactions for amplification and accurate qPCR profiling.

 SeraMir provides reliably better qPCR profiling than exosomal RNAs isolated using phenol/trizol

Figure 2. Serum exoRNAs prepared using SeraMir deliver excellent performance in microarray studies. Samples from a pooled normal serum preparation and from a male caucasian (age 73) with adenocarcinoma of the colon were used in this study. Exosomes were precipitated from 250 µL of serum using the SeraMir Exosome RNA Amplification Kit. The T7-amplified “sense” exoRNAs were then used for direct labeling analyses on LC Sciences miRBase ver.16 array chips (performed in triplicate). The exoRNAs were hybridized across 1,214 different microRNAs on the probe set. Of the 1,214 microRNAs analyzed, 79 microRNAs showed a signal intensity >32. Within this set of 79, there was a clear colon versus normal “signature set” of 40 microRNAs that could discriminate normal from colon cancer serum samples with a p-value < 0.01. The identities of the microRNAs found in this study have been masked while further investigation continues.

Citations

  • Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. ;. Link: Conference
  • Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. ;. Link: Abstract
  • Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017 Apr 15;. PM ID: 28411393
  • Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017 Jan 1; 25(1):102-110. PM ID: 27883272
  • Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017 Apr 1; 31(9):1277-1295. PM ID: 28447547
  • Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017 Feb 1; 15:163-172. PM ID: 27974246
  • Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017 Jun 13;. PM ID: 28623135
  • Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017 Jun 4;. PM ID: 28580875
  • Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017 Jun 1;:95-99. PM ID: 28643924
  • Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017 May 1; 4(2):112-121. PM ID: 28451447
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product
  • Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. ; 7(4). Link: bio-protocol
  • Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017 Jan 1; 21(1):55-66. PM ID: 28271982
  • Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017 Jun 27; 8(26):42712-42727. PM ID: 28514744
  • Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017 Apr 5;. PM ID: 28157838
  • Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016 Feb 1; 429:39-49. PM ID: 26723490
  • Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. ; 5(1):1–94. Link: Liver Cancer
  • Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016 Mar 1; 5:30924. PM ID: 26928673
  • Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016 Mar 2; 11(3):e0150154. PM ID: 26930277
  • Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016 Jul 20; 39:427-432. Link: 中华检验医学杂志