Rab5b Exo-Flow Capture Kit

For the selective capture and flow sorting (FACS) of exosome subpopulations based on the presence of Rab5b, which is involved in membrane transport and fusion
  • A range of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Reversible Exo-FITC and Exo-APC stains can be completely removed after FACS
  • Exosome Elution Buffer simultaneously removes Exo-FITC (or Exo-APC) and elutes exosomes from the magnetic beads for downstream applications such as functional studies

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOFLOW500A-1 Rab5b Exo-Flow capture kit (Magnetic streptavidin beads, Rab5b-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) 10 Reactions $479.00
- +
Contact Us

Overview

Overview

Go with the flow for easy exosome isolation using FACS The Rab5b Exo-Flow Capture Kit has all the reagents you need to purify exosomes using FACS—magnetic streptavidin beads, Rab5b-biotin capture antibody, wash and elution buffers, and reversible Exo-FITC stain (Cat.# EXOFLOW800A-1)—you supply the FACS and the optional magnetic stand (Cat.# EXOFLOW700A-1). Our well-validated Rab5b-biotin antibody and high-quality kit components ensure reliable, reproducible exosome purification based on the presence of Rab5b on the exosome surface. And with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations. Note that we also offer reversible Exo-APC (Cat.# Exo-FLOW810A-1) as an alternative stain to Exo-FITC.
  • A range of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Reversible Exo-FITC and Exo-APC stains can be completely removed after FACS
  • Exosome Elution Buffer simultaneously removes Exo-FITC (or Exo-APC) and elutes exosomes from the magnetic beads for downstream applications such as functional studies
Exosome capture is highly efficient with large bead sizeMore than just Rab5bs To facilitate the widest range of studies, SBI built the Exo-Flow system to be highly modular. We offer a range of biotinylated antibodies as well as a Basic Exo-Flow Kit (Cat.# CSFLOWBASICA-1) with no antibody so that you can use your own biotinylated antibodies.
Cat.#Kit
EXOFLOW100A-1CD9 Exo-Flow Capture Kit
EXOFLOW150A-1Tetraspanin Exo-Flow Combo Capture Kit
EXOFLOW200A-1CD31 Exo-Flow Capture Kit
EXOFLOW210A-1CD44 Exo-Flow Capture Kit
EXOFLOW300A-1CD63 Exo-Flow Capture Kit
EXOFLOW400A-1CD81 Exo-Flow Capture Kit
EXOFLOW500A-1Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1CD14 Exo-Flow Capture Kit
EXOFLOW620A-1CD68 Exo-Flow Capture Kit
EXOFLOW630A-1EpCAM Exo-Flow Capture Kit
EXOFLOW660A-1CD73 Exo-Flow Capture Kit

How It Works

How It Works

Easily purify exosomes using FACS with the Rab5b Exo-Flow Capture Kit

At a glance
Simply (1) couple the Rab5b-biotin antibody to the magnetic streptavidin beads, (2) use the Rab5b-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).

Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.

How the Exo-Flow Capture Kit’s magnetic stand works

Supporting Data

Supporting Data

See our Rab5b Exo-FLOW Capture Kit in action

Highly selective exosome isolation by FACS using anti-Rab5b

Our Rab5b Exo-Flow Capture Kit delivers quantitative, highly selective exosome isolationBead flow separation data for exosomes secreted by HEK293 cells and captured using our Rab5b Exo-Flow Capture Kit. Plots of forward scatter versus FITC intensity show that in the no exosome control, only 0.5% of particles are FITC-positive (left panel), whereas in the exosome-containing sample, 99.3% of particles are FITC-positive (middle panel). The degree of flow separation is shown in the right panel.
Human serum exosomes were isolated from 250 µL serum using ExoQuick® and the exosome pellet resuspended in 500 µL of 1x PBS. Exosome particles were added as two-fold dilutions starting at 50 µL, and then captured using the biotinylated antibody coupled to Exo-Flow beads. The FITC flow cytometric intensities are then plotted versus the number of exosome particles (determined using NanoSight).

Resources

Citations

  • Narayanan, M, et al. (2021) Cocaine augments neuro-inflammation via modulating extracellular vesicle release in HIV-1 infected immune cells. Retrovirology. 1970 Jan 1; 18(1):26. PM ID: 34530855
  • Krishnamachary, B, et al. (2021) Extracellular vesicle TGF-β1 is linked to cardiopulmonary dysfunction in HIV. American journal of respiratory cell and molecular biology. 1970 Jan 1;. PM ID: 34014809
  • Ali, A, et al. (2021) Hyperoxia-activated circulating extracellular vesicles induce lung and brain injury in neonatal rats. Scientific reports. 1970 Jan 1; 11(1):8791. PM ID: 33888735
  • Reale, A, et al. (2021) Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2261:151-191. PM ID: 33420989
  • Zhang, X, et al. (2020) Programmable Extracellular Vesicles for Macromolecule Delivery and Genome Modifications. Developmental cell. 1970 Jan 1; 55(6):784-801.e9. PM ID: 33296682
  • Guerra, M. (2020) From the plasma membrane to tangled DNA webs: a roadway to track, investigate and employ spatially localized neutrophil elastase and cathepsin G activities. Thesis. 1970 Jan 1;. Link: Thesis
  • Melnikov, I, et al. (2020) CRP Is Transported by Monocytes and Monocyte-Derived Exosomes in the Blood of Patients with Coronary Artery Disease. Biomedicines. 1970 Jan 1; 8(10). PM ID: 33086769
  • Woo, C, et al. (2020) Small extracellular vesicles from human adipose-derived stem cells attenuate cartilage degeneration. Journal of Extracellular Vesicles. 1970 Jan 1; 9(1):1735249. Link: Journal of Extracellular Vesicles
  • Velandia-Romero, ML, et al. (2020) Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926. PLoS ONE. 1970 Jan 1; 15(1):e0227030. PM ID: 31910224
  • Bauer, S. (2019) Modifizierung und Charakterisierung von Exosomen als therapeutisches Transportsystem. Thesis. 1970 Jan 1;. Link: Thesis
  • Khalyfa, A, et al. (2019) Plasma Exosomes in OSA Patients Promote Endothelial Senescence: Effect of Long-Term Adherent Continuous Positive Airway Pressure. Sleep. 1970 Jan 1;. PM ID: 31552414
  • Sheller-Miller, S, et al. (2019) Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Sci Rep. 2019 Jan 24; 9(1):608. PM ID: 30679631
  • Sheller-Miller, S, et al. (2019) Cre-Reporter Mouse Model to Determine Exosome Communication and Function during Pregnancy. Am. J. Obstet. Gynecol.. 2019 Jun 14;. PM ID: 31207235
  • Genschmer, KR, et al. (2019) Activated PMN Exosomes: Pathogenic Entities Causing Matrix Destruction and Disease in the Lung. Cell. 2019 Jan 10; 176(1-2):113-126.e15. PM ID: 30633902
  • Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm.. 2017 Jun 21; 2017:4814987. PM ID: 28634420
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product
  • Meyer, C, et al. (2017) Pseudotyping exosomes for enhanced protein delivery in mammalian cells.. Int J Nanomedicine. 2017 May 1; 12:3153-3170. PM ID: 28458537
  • Chen, C, et al. (2017) Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis. Cell Res.. 2017 Apr 1; 27(4):559-577. PM ID: 28106077
  • Etzerodt, A, et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma. Sci Rep. 2017 Jan 13; 7:40286. PM ID: 28084321
  • Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry. J Cancer Prev. 2016 Sep 1; 21(3):194-200. PM ID: 27722146

Products

Catalog Number Description Size Price Quantity Add to Cart
EXOFLOW500A-1 Rab5b Exo-Flow capture kit (Magnetic streptavidin beads, Rab5b-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain) 10 Reactions $479.00
- +
Contact Us

Overview

Overview

Go with the flow for easy exosome isolation using FACS The Rab5b Exo-Flow Capture Kit has all the reagents you need to purify exosomes using FACS—magnetic streptavidin beads, Rab5b-biotin capture antibody, wash and elution buffers, and reversible Exo-FITC stain (Cat.# EXOFLOW800A-1)—you supply the FACS and the optional magnetic stand (Cat.# EXOFLOW700A-1). Our well-validated Rab5b-biotin antibody and high-quality kit components ensure reliable, reproducible exosome purification based on the presence of Rab5b on the exosome surface. And with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations. Note that we also offer reversible Exo-APC (Cat.# Exo-FLOW810A-1) as an alternative stain to Exo-FITC.
  • A range of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Reversible Exo-FITC and Exo-APC stains can be completely removed after FACS
  • Exosome Elution Buffer simultaneously removes Exo-FITC (or Exo-APC) and elutes exosomes from the magnetic beads for downstream applications such as functional studies
Exosome capture is highly efficient with large bead sizeMore than just Rab5bs To facilitate the widest range of studies, SBI built the Exo-Flow system to be highly modular. We offer a range of biotinylated antibodies as well as a Basic Exo-Flow Kit (Cat.# CSFLOWBASICA-1) with no antibody so that you can use your own biotinylated antibodies.
Cat.#Kit
EXOFLOW100A-1CD9 Exo-Flow Capture Kit
EXOFLOW150A-1Tetraspanin Exo-Flow Combo Capture Kit
EXOFLOW200A-1CD31 Exo-Flow Capture Kit
EXOFLOW210A-1CD44 Exo-Flow Capture Kit
EXOFLOW300A-1CD63 Exo-Flow Capture Kit
EXOFLOW400A-1CD81 Exo-Flow Capture Kit
EXOFLOW500A-1Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1CD14 Exo-Flow Capture Kit
EXOFLOW620A-1CD68 Exo-Flow Capture Kit
EXOFLOW630A-1EpCAM Exo-Flow Capture Kit
EXOFLOW660A-1CD73 Exo-Flow Capture Kit

How It Works

How It Works

Easily purify exosomes using FACS with the Rab5b Exo-Flow Capture Kit

At a glance
Simply (1) couple the Rab5b-biotin antibody to the magnetic streptavidin beads, (2) use the Rab5b-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).

Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.

How the Exo-Flow Capture Kit’s magnetic stand works

Supporting Data

Supporting Data

See our Rab5b Exo-FLOW Capture Kit in action

Highly selective exosome isolation by FACS using anti-Rab5b

Our Rab5b Exo-Flow Capture Kit delivers quantitative, highly selective exosome isolationBead flow separation data for exosomes secreted by HEK293 cells and captured using our Rab5b Exo-Flow Capture Kit. Plots of forward scatter versus FITC intensity show that in the no exosome control, only 0.5% of particles are FITC-positive (left panel), whereas in the exosome-containing sample, 99.3% of particles are FITC-positive (middle panel). The degree of flow separation is shown in the right panel.
Human serum exosomes were isolated from 250 µL serum using ExoQuick® and the exosome pellet resuspended in 500 µL of 1x PBS. Exosome particles were added as two-fold dilutions starting at 50 µL, and then captured using the biotinylated antibody coupled to Exo-Flow beads. The FITC flow cytometric intensities are then plotted versus the number of exosome particles (determined using NanoSight).

Citations

  • Narayanan, M, et al. (2021) Cocaine augments neuro-inflammation via modulating extracellular vesicle release in HIV-1 infected immune cells. Retrovirology. 1970 Jan 1; 18(1):26. PM ID: 34530855
  • Krishnamachary, B, et al. (2021) Extracellular vesicle TGF-β1 is linked to cardiopulmonary dysfunction in HIV. American journal of respiratory cell and molecular biology. 1970 Jan 1;. PM ID: 34014809
  • Ali, A, et al. (2021) Hyperoxia-activated circulating extracellular vesicles induce lung and brain injury in neonatal rats. Scientific reports. 1970 Jan 1; 11(1):8791. PM ID: 33888735
  • Reale, A, et al. (2021) Human Plasma Extracellular Vesicle Isolation and Proteomic Characterization for the Optimization of Liquid Biopsy in Multiple Myeloma. Methods in molecular biology (Clifton, N.J.). 1970 Jan 1; 2261:151-191. PM ID: 33420989
  • Zhang, X, et al. (2020) Programmable Extracellular Vesicles for Macromolecule Delivery and Genome Modifications. Developmental cell. 1970 Jan 1; 55(6):784-801.e9. PM ID: 33296682
  • Guerra, M. (2020) From the plasma membrane to tangled DNA webs: a roadway to track, investigate and employ spatially localized neutrophil elastase and cathepsin G activities. Thesis. 1970 Jan 1;. Link: Thesis
  • Melnikov, I, et al. (2020) CRP Is Transported by Monocytes and Monocyte-Derived Exosomes in the Blood of Patients with Coronary Artery Disease. Biomedicines. 1970 Jan 1; 8(10). PM ID: 33086769
  • Woo, C, et al. (2020) Small extracellular vesicles from human adipose-derived stem cells attenuate cartilage degeneration. Journal of Extracellular Vesicles. 1970 Jan 1; 9(1):1735249. Link: Journal of Extracellular Vesicles
  • Velandia-Romero, ML, et al. (2020) Extracellular vesicles of U937 macrophage cell line infected with DENV-2 induce activation in endothelial cells EA.hy926. PLoS ONE. 1970 Jan 1; 15(1):e0227030. PM ID: 31910224
  • Bauer, S. (2019) Modifizierung und Charakterisierung von Exosomen als therapeutisches Transportsystem. Thesis. 1970 Jan 1;. Link: Thesis
  • Khalyfa, A, et al. (2019) Plasma Exosomes in OSA Patients Promote Endothelial Senescence: Effect of Long-Term Adherent Continuous Positive Airway Pressure. Sleep. 1970 Jan 1;. PM ID: 31552414
  • Sheller-Miller, S, et al. (2019) Exosomes Cause Preterm Birth in Mice: Evidence for Paracrine Signaling in Pregnancy. Sci Rep. 2019 Jan 24; 9(1):608. PM ID: 30679631
  • Sheller-Miller, S, et al. (2019) Cre-Reporter Mouse Model to Determine Exosome Communication and Function during Pregnancy. Am. J. Obstet. Gynecol.. 2019 Jun 14;. PM ID: 31207235
  • Genschmer, KR, et al. (2019) Activated PMN Exosomes: Pathogenic Entities Causing Matrix Destruction and Disease in the Lung. Cell. 2019 Jan 10; 176(1-2):113-126.e15. PM ID: 30633902
  • Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm.. 2017 Jun 21; 2017:4814987. PM ID: 28634420
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product
  • Meyer, C, et al. (2017) Pseudotyping exosomes for enhanced protein delivery in mammalian cells.. Int J Nanomedicine. 2017 May 1; 12:3153-3170. PM ID: 28458537
  • Chen, C, et al. (2017) Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis. Cell Res.. 2017 Apr 1; 27(4):559-577. PM ID: 28106077
  • Etzerodt, A, et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma. Sci Rep. 2017 Jan 13; 7:40286. PM ID: 28084321
  • Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry. J Cancer Prev. 2016 Sep 1; 21(3):194-200. PM ID: 27722146