CD14 Exo-Flow Capture Kit

For the selective capture and flow sorting (FACS) of exosome subpopulations based on the presence of CD14

Description
Size
Catalog Number
Price
Quantity
Add to Cart

CD14 Exo-Flow capture kit (Magnetic streptavidin beads, CD14-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain)

Array ( [attributes] => Array ( [attribute_size] => 10 Reactions ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 450 [display_regular_price] => 450 [image] => Array ( [title] => cd14-exo-flow-kit [caption] => [url] => https://www.systembio.com/wp-content/uploads/exosome-research/cd14-exo-flow-kit.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/exosome-research/cd14-exo-flow-kit.png [srcset] => https://www.systembio.com/wp-content/uploads/exosome-research/cd14-exo-flow-kit.png 345w, https://www.systembio.com/wp-content/uploads/exosome-research/cd14-exo-flow-kit-300x300.png 300w, https://www.systembio.com/wp-content/uploads/exosome-research/cd14-exo-flow-kit-100x100.png 100w [sizes] => (max-width: 345px) 100vw, 345px [full_src] => https://www.systembio.com/wp-content/uploads/exosome-research/cd14-exo-flow-kit.png [full_src_w] => 345 [full_src_h] => 345 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/exosome-research/cd14-exo-flow-kit-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/exosome-research/cd14-exo-flow-kit-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 345 [src_h] => 345 ) [image_id] => 7542 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $450 [sku] => EXOFLOW610A-1 [variation_description] =>

CD14 Exo-Flow capture kit (Magnetic streptavidin beads, CD14-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain)

[variation_id] => 63736 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
10 Reactions
EXOFLOW610A-1
$ 450
Contact Us Speak to a specialist
1-888-266-5066

Overview

Go with the flow for easy exosome isolation using FACS

The CD14 Exo-Flow Capture Kit has all the reagents you need to purify exosomes using FACS—magnetic streptavidin beads, CD14-biotin capture antibody, wash and elution buffers, and reversible Exo-FITC stain (Cat.# EXOFLOW800A-1)—you supply the FACS and the optional magnetic stand (Cat.# EXOFLOW700A-1). Our well-validated CD14-biotin antibody and high-quality kit components ensure reliable, reproducible exosome purification based on the presence of CD14 on the exosome surface. And with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations.

Note that we also offer reversible Exo-APC (Cat.# Exo-FLOW810A-1) as an alternative stain to Exo-FITC.

  • A range of well-validated antibodies for reliable and reproducible purification
  • Large-sized magnetic beads increase the efficiency of exosome capture
  • Reversible Exo-FITC and Exo-APC stains can be completely removed after FACS
  • Exosome Elution Buffer simultaneously removes Exo-FITC (or Exo-APC) and elutes exosomes from the magnetic beads for downstream applications such as functional studies

Exosome capture is highly efficient with large bead size

More than just CD14s

To facilitate the widest range of studies, SBI built the Exo-Flow system to be highly modular. We offer a range of biotinylated antibodies as well as a Basic Exo-Flow Kit (Cat.# CSFLOWBASICA-1 with no antibody so that you can use your own biotinylated antibodies.

Cat.# Kit
EXOFLOW100A-1 CD9 Exo-Flow Capture Kit
EXOFLOW150A-1 Tetraspanin Exo-Flow Combo Capture Kit

EXOFLOW200A-1 CD31 Exo-Flow Capture Kit
EXOFLOW210A-1 CD44 Exo-Flow Capture Kit
EXOFLOW300A-1 CD63 Exo-Flow Capture Kit
EXOFLOW400A-1 CD81 Exo-Flow Capture Kit
EXOFLOW500A-1 Rab5b Exo-Flow Capture Kit
EXOFLOW600A-1 HLA-G Exo-Flow Capture Kit
EXOFLOW610A-1 CD14 Exo-Flow Capture Kit
EXOFLOW620A-1 CD68 Exo-Flow Capture Kit
EXOFLOW630A-1 EpCAM Exo-Flow Capture Kit
EXOFLOW650A-1 α2-Macroglobulin (A2M) Exo-Flow Capture Kit
EXOFLOW660A-1 CD73 Exo-Flow Capture Kit

How It Works

Easily purify exosomes using FACS with the CD14 Exo-Flow Capture Kit

At a glance
Simply (1) couple the CD14-biotin antibody to the magnetic streptavidin beads, (2) use the CD14-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).

Your sample is now ready for FACS analysis.

To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.

How the Exo-Flow Capture Kit’s magnetic stand works

Supporting Data

See our Exo-FLOW Capture Kits in action

Highly selective exosome isolation by FACS

Our Exo-Flow Capture Kits deliver quantitative, highly selective exosome isolation. Bead flow separation data for exosomes secreted by HEK293 cells and captured using our different Exo-Flow Capture Kits. Plots of forward scatter versus FITC intensity show that in the no exosome control, only 0.5% of particles are FITC-positive (left panels), whereas in the exosome-containing sample, 99.3% of particles are FITC-positive (middle panels). The degree of flow separation is shown in the right panels.
Human serum exosomes were isolated from 250 µL serum using ExoQuick® and the exosome pellet resuspended in 500 µL of 1x PBS. Exosome particles were added as two-fold dilutions starting at 50 µL, and then captured using the biotinylated antibody coupled to Exo-Flow beads. The FITC flow cytometric intensities are then plotted versus the number of exosome particles (determined using NanoSight).


Citations

  • Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm.. 2017 Jun 21; 2017:4814987. PM ID: 28634420
  • Meyer, C, et al. (2017) Pseudotyping exosomes for enhanced protein delivery in mammalian cells.. Int J Nanomedicine. 2017 May 1; 12:3153-3170. PM ID: 28458537
  • Chen, C, et al. (2017) Mesenchymal stem cell transplantation in tight-skin mice identifies miR-151-5p as a therapeutic target for systemic sclerosis. Cell Res.. 2017 Apr 1; 27(4):559-577. PM ID: 28106077
  • Etzerodt, A, et al. (2017) Soluble ectodomain CD163 and extracellular vesicle-associated CD163 are two differently regulated forms of ‘soluble CD163’ in plasma. Sci Rep. 2017 Jan 13; 7:40286. PM ID: 28084321
  • Smith, JA & Daniel, R. (2016) Human vaginal fluid contains exosomes that have an inhibitory effect on an early step of the HIV-1 life cycle. AIDS. 2016 Nov 13; 30(17):2611-2616. PM ID: 27536982
  • Rim, KT & Kim, SJ. (2016) Quantitative Analysis of Exosomes From Murine Lung Cancer Cells by Flow Cytometry. J Cancer Prev. 2016 Sep 1; 21(3):194-200. PM ID: 27722146
  • Junker, K, et al. (2016) Extracellular Vesicles and Their Role in Urologic Malignancies.. Eur. Urol.. 2016 Aug 1; 70(2):323-31. PM ID: 26924769
  • Mukherjee, K, et al. (2016) Reversible HuR-microRNA binding controls extracellular export of miR-122 and augments stress response. EMBO Rep.. 2016 Aug 1; 17(8):1184-203. PM ID: 27402548
  • Wen, Z, et al. (2016) NADPH oxidase deficiency underlies dysfunction of aged CD8+ Tregs. J. Clin. Invest.. 2016 May 2; 126(5):1953-67. PM ID: 27088800
  • Raimondo, F, et al. (2016) Urinary proteomics for the study of genetic kidney diseases.. Expert Rev Proteomics. 2016 Mar 2; 13(3):309-24. PM ID: 26698090
  • Peterson, MF, et al. (2015) Integrated systems for exosome investigation. Methods. 2015 Oct 1; 87:31-45. PM ID: 25916618
  • Nudel, K, Massari, P & Genco, CA. (2015) Neisseria gonorrhoeae Modulates Cell Death in Human Endocervical Epithelial Cells through Export of Exosome-Associated cIAP2. Infect. Immun.. 2015 Sep 1; 83(9):3410-7. PM ID: 26077759
  • Bhattarai, N, et al. (2015) Conserved Motifs within Hepatitis C Virus Envelope (E2) RNA and Protein Independently Inhibit T Cell Activation. PLoS Pathog.. 2015 Sep 1; 11(9):e1005183. PM ID: 26421924
  • Santana, SM, et al. (2014) Cancerous epithelial cell lines shed extracellular vesicles with a bimodal size distribution that is sensitive to glutamine inhibition. Phys Biol. 2014 Nov 26; 11(6):065001. PM ID: 25426818
  • Di Noto, G, et al. (2014) Immunoglobulin Free Light Chains and GAGs Mediate Multiple Myeloma Extracellular Vesicles Uptake and Secondary NfκB Nuclear Translocation. Front Immunol. 2014 Nov 11; 5:517. PM ID: 25386176
  • Basu, S & Bhattacharyya, SN. (2014) Insulin-like growth factor-1 prevents miR-122 production in neighbouring cells to curtail its intercellular transfer to ensure proliferation of human hepatoma cells. Nucleic Acids Res.. 2014 Jun 1; 42(11):7170-85. PM ID: 24813441
  • Jia, S, et al. (2014) Emerging technologies in extracellular vesicle-based molecular diagnostics. Expert Rev. Mol. Diagn.. 2014 Apr 1; 14(3):307-21. PM ID: 24575799
  • Bednarczyk, M, Biryło, M & DAWIDOWICZ, A. (2015) Modern Geodetic Techniques in Spatial Measurement. Thesis. ;. Link: Thesis
  • Nudel, K. (2016) Neisseria gonorrhoeae modulates epithelial cell responses via the induction and release of the inhibitor of apoptosis protein cIAP2 in exosomes. Thesis. ;. Link: Thesis
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product