TransDuxTM MAX Lentivirus Transduction Reagent

Maximize transduction efficiencies for more reliable and reproducible gene delivery, even in infection-resistant cell types

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TransDux MAX Lentivirus Transduction Enhancer

100 Transductions
LV860A-1
$352

Overview

Be confident in your gene delivery with TransDux MAXTM

Whether you’re working with difficult-to-transduce cells like primary T-cells or just want to ensure high transduction efficiency, SBI’s TransDux™ reagents are ready to deliver. Our newer TransDux MAX Lentiviral Transduction Reagent can increase transduction efficiencies by up to 8-fold compared to polybrene, while our original TransDux formulation (Cat# LV850A-1) is still available for researchers who are not yet ready to make the change.

Ensure reliable and efficient gene delivery with TransDux MAX.

  • Efficient—up to 8-fold greater transduction efficiency than polybrene, and 4-fold greater efficiency than standard TransDux reagent as measured by qPCR*
  • Easy-to-use—kit format requires minimal hands-on time (<5 minutes)
  • Non-toxic—no need to change out media after infection
  • Versatile—works with all types of packaged lentivirus
  • Flexible—enhances transduction efficiency in a wide variety of cell lines
  • Broadly compatible—does not interfere with downstream gene expression or functional assays

*Based on infection of HT1080 cells with the pre-packaged virus format of SBI’s BLIV 2.0 reporter MSCV-Luciferase-EF1a-copGFP-T2A-Puro (Cat# BLIV713VA-1) and the appropriate conditions for polybrene, TransDux, and TransDux MAX. Virus transduction & integration efficiency was measured using SBI’s Global UltraRapidTM Titering Kit (Cat #LV961A-1)

How It Works

Take your transduction efficiencies to a whole new level

TransDux MAX comes in an easy-to-use, two component format (TransDux and MAX Enhancer) good for 100 transduction reactions*. Simply mix both reagents into conditioned media from target cells (Figure 1), and infect with your lentivirus of choice for a higher level of transduction efficiency.

TransDux MAX has a quick and easy workflow

Figure 1. TransDux MAX has a quick and easy workflow.

*One reaction is defined as treating 500 µl of conditioned media in a single well of 24-well plate

Supporting Data

Maximize transduction efficiencies with TransDux MAX

Increase transduction efficiencies with TransDux

Figure 2. TransDux MAX delivers even higher transduction efficiencies than the original TransDux reagent. SBI’s BLIV713 pre-packaged lentivirus (CMV-Luciferase-EF1a-copGFP-T2A-Puro) was transduced into HT1080 cells using either TransDux MAX, TransDux, or polybrene. Cells were imaged for GFP expression 72-hours post-infection. (Left panel) Microscopy of transduced cells. (Right panel) qPCR-based quantitation of virus infectivity. TransDux MAX provides 4-fold higher infectivity than the original TransDux Reagent, and 8-fold higher infectivity than polybrene.

TransDux MAX delivers high transduction efficiencies across a broad range of cell types.

Figure 3. TransDux MAX delivers high transduction efficiencies across a broad range of cell types. Transduction of SBI’s LV605 pre-packaged virus (MSCV-GFP-T2A-RFP) into various cell lines (HT1080, HepG2, and Jurkat T-cells). Cells were imaged for RFP expression 72-hours post-infection. TransDux MAX clearly delivers more RFP-positive cells than TransDux in all three cell lines tested.

TransDux MAX works with hard-to-transduce lentivectors.

Figure 4. TransDux MAX works with hard-to-transduce lentivectors. Transduction of a custom lentivirus construct expressing GFP and a transgene-of-interest into HT1080 cells using either TransDux MAX (left panel) or the original TransDux reagent (right panel). Cells were imaged for GFP expression 72-hours post-infection. TransDux MAX is able to deliver transduced cells even when the lentivector is too difficult for the original TransDux reagent to transduce.