EXOCET Exosome Quantitation Kit

Find out how many extracellular vesicles you have in just 20 minutes with this antibody-free, colorimetric assay

Description
Size
Catalog Number
Price
Quantity
Add to Cart

EXOCET Exosome Quantitation Kit

Array ( [attributes] => Array ( [attribute_size] => 96 Reactions ) [availability_html] => [backorders_allowed] => [dimensions] => Array ( [length] => [width] => [height] => ) [dimensions_html] => N/A [display_price] => 415 [display_regular_price] => 415 [image] => Array ( [title] => exosome-research-general-01 [caption] => [url] => https://www.systembio.com/wp-content/uploads/exosome-research/exosome-research-general-01.png [alt] => [src] => https://www.systembio.com/wp-content/uploads/exosome-research/exosome-research-general-01.png [srcset] => https://www.systembio.com/wp-content/uploads/exosome-research/exosome-research-general-01.png 345w, https://www.systembio.com/wp-content/uploads/exosome-research/exosome-research-general-01-300x300.png 300w, https://www.systembio.com/wp-content/uploads/exosome-research/exosome-research-general-01-100x100.png 100w [sizes] => (max-width: 345px) 100vw, 345px [full_src] => https://www.systembio.com/wp-content/uploads/exosome-research/exosome-research-general-01.png [full_src_w] => 345 [full_src_h] => 345 [gallery_thumbnail_src] => https://www.systembio.com/wp-content/uploads/exosome-research/exosome-research-general-01-100x100.png [gallery_thumbnail_src_w] => 100 [gallery_thumbnail_src_h] => 100 [thumb_src] => https://www.systembio.com/wp-content/uploads/exosome-research/exosome-research-general-01-300x300.png [thumb_src_w] => 300 [thumb_src_h] => 300 [src_w] => 345 [src_h] => 345 ) [image_id] => 7579 [is_downloadable] => [is_in_stock] => 1 [is_purchasable] => 1 [is_sold_individually] => no [is_virtual] => [max_qty] => [min_qty] => 1 [price_html] => $415 [sku] => EXOCET96A-1 [variation_description] =>

EXOCET Exosome Quantitation Kit

[variation_id] => 63690 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
96 Reactions
EXOCET96A-1
$ 415
Contact Us Speak to a specialist
1-888-266-5066

Overview

Move quickly with SBI’s fastest exosome quantitation method

With the EXOCET Exosome Quantitation Kit, you can find out just how many exosomes you’ve isolated in as little as 20 minutes. This antibody-free, colorimetric assay (OD405) measures the activity of Acetyl-CoA Acetylcholinesterase (AChE), an enzyme shown to be highly enriched in exosomes1, 2 (using mass spectrometry and Western blotting, SBI has verified the presence of AChE in exosomes from serum, stem cells, cancer cell lines, and MSCs—contact tech@systembio.com to learn more).

EXOCET is a highly quantitative assay and the standards included with each kit have been calibrated using NanoSight analysis, enabling calculation of the number of exosomes in your samples. The EXOCET Kit contains all of the necessary assay reagents and an optical 96-well plate to perform 96 reactions.

  • Fast—complete in 20 minutes
  • Antibody-free—quantitation is based on the activity of AChE, an enzyme enriched in most exosomes
  • Quantitative—included calibration standards enable calculation of the number of exosome particles
  • Flexible—works with all mammalian species tested (human, mouse, rat)
  • Fully compatible—works with most exosome isolation methods including the ExoQuick family of reagents, ultracentrifugation, immunoaffinity purification, and chromatography

Choose the exosome quantitation method that’s best for your studies

  ExoELISA-ULTRA CD63
ExoELISA-ULTRA CD81
ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet
Use For fast and sensitive antibody-based quantitation of exosomes For sensitive quantitation of exosomes when time and input sample are not limiting For fast quantitation of extracellular vesicles with moderate sample input requirements For the most sensitive quantitation of extracellular vesicles with very low sample input requirements
Detection method Antibody Antibody Enzymatic Enzymatic
Quantitation chemistry Enzymatic (HRP) Enzymatic (HRP) Colorimetric Fluorescent
Total protocol time 4 hours (no overnight incubation) 24 hours 20 min 60 min
Input sample amount (protein equivalent) 1 – 200 µg >500 µg 50 µg <1 µg
Learn More ExoELISA-ULTRA CD63

ExoELISA-ULTRA CD81

ExoELISA CD9
ExoELISA CD63
ExoELISA CD81
EXOCET FluoroCet

REFERENCES

  1. Savina A, et al. The exosome pathway in K562 cells is regulated by Rab11. J Cell Sci. 2002 Jun 15. 115(Pt 12):2505-15. PMID: 12045221.
  2. Gupta S, and Knowlton AA. HSP60 trafficking in adult cardiac myocytes: role of the exosomal pathway. Am J Physiol Heart Circ Physiol. 2007 Jun. 292(6):H3052-6. PMID: 17307989.

How It Works

Exosome quantitation with EXOCET is quick and easy

  1. Isolate exosomes (ExoQuick, ExoQuick-TC, ExoQuick PLUS, and ExoQuick-TC PLUS are all excellent methods)
  2. Lyse exosomes with the included Exosome Lysis Buffer
  3. Measure AChE activity—add the included buffer and incubate for 10–20 minutes. Readout is at 405 nm

Supporting Data

The fast, highly quantitative EXOCET exosome quantitation assay

Sample data and standard curve for EXOCET.

Exosome quantitation with EXOCET takes as little as 20 minutes and 50 µg (protein equivalent) of sample. Exosomes were isolated from either human serum (0.5 mL) or from MDA-MB-231 culture media (10 mL) using standard ExoQuick and ExoQuick-TC reagents. Exosome pellets were resuspended in PBS and total protein concentration measured using a BCA assay (~108 exosomes, 2 µg/µL). Exosomes were lysed using the EXOCET gentle lysis solution to preserve the enzymatic activity of the exosomal AChE enzyme. The standard curve was generated using known numbers of exosomes (as measured by NanoSight) and calibrated with a recombinant AChE enzyme standard solution provided in the kit.

Citations

  • Slowey, PD. (2017) Salivary Diagnostics Using Purified Nucleic Acids. Methods Mol. Biol.. 2017 Dec 7; 1537:3-15. PM ID: 27924585
  • Wu, X, et al. (2017) Tongxinluo Inhibits Renal Fibrosis in Diabetic Nephropathy: Involvement of the Suppression of Intercellular Transfer of TGF-ββ1-Containing Exosomes from GECs to GMCs Read More: http://www.worldscientific.com/doi/abs/10.1142/S0192415X17500586. The American Journal of Chinese Medicine. 2017 Jun 29;. Link: The American Journal of Chinese Medicine
  • Sharma, R, et al. (2017) Detection of phosphatidylserine-positive exosomes for the diagnosis of early-stage malignancies. Br. J. Cancer. 2017 Jun 22;. PM ID: 28641308
  • Domenis, R, et al. (2017) Characterization of the Proinflammatory Profile of Synovial Fluid-Derived Exosomes of Patients with Osteoarthritis. Mediators Inflamm.. 2017 Jun 21; 2017:4814987. PM ID: 28634420
  • Rody, WJ, et al. (2017) The use of cell culture platforms to identify novel markers of bone and dentin resorption. Orthod Craniofac Res. 2017 Jun 1;:89-94. PM ID: 28643914
  • Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017 Apr 5;. PM ID: 28157838
  • Jiang, X, et al. (2017) Exosomes from pediatric dilated cardiomyopathy patients modulate a pathological response in cardiomyocytes. Am. J. Physiol. Heart Circ. Physiol.. 2017 Apr 1; 312(4):H818-H826. PM ID: 28130338
  • Hurwitz, SN, et al. (2017) CD63 Regulates Epstein-Barr Virus LMP1 Exosomal Packaging, Enhancement of Vesicle Production, and Noncanonical NF-κB Signaling. J. Virol.. 2017 Mar 1; 91(5). PM ID: 27974566
  • Thomou, T, et al. (2017) Adipose-derived circulating miRNAs regulate gene expression in other tissues. Nature. 2017 Feb 23; 542(7642):450-455. PM ID: 28199304
  • Lee, JY, et al. (2016) Circulating exosomes from patients with systemic lupus erythematosus induce an proinflammatory immune response. Arthritis Res. Ther.. 2016 Nov 16; 18(1):264. PM ID: 27852323
  • Rossaint, J, et al. (2016) Directed transport of neutrophil-derived extracellular vesicles enables platelet-mediated innate immune response. Nat Commun. 2016 Nov 15; 7:13464. PM ID: 27845343
  • Matsumoto, Y, et al. (2016) Quantification of plasma exosome is a potential prognostic marker for esophageal squamous cell carcinoma. Oncol. Rep.. 2016 Nov 1; 36(5):2535-2543. PM ID: 27599779
  • Gutierrez, MJ, et al. (2016) Airway Secretory microRNAome Changes during Rhinovirus Infection in Early Childhood. PLoS ONE. 2016 Sep 20; 11(9):e0162244. PM ID: 27643599
  • Yang, J, et al. (2016) Anomalous uptake and circulatory characteristics of the plant-based small RNA MIR2911. Sci Rep. 2016 Jun 2; 6:26834. PM ID: 27251858
  • Huynh, N, et al. (2016) Characterization of Regulatory Extracellular Vesicles from Osteoclasts. J. Dent. Res.. 2016 Jun 1; 95(6):673-9. PM ID: 26908631
  • Wu, XM, et al. (2016) Exosomes from high glucose-treated glomerular endothelial cells activate mesangial cells to promote renal fibrosis. Biol Open. 2016 Apr 15; 5(4):484-91. PM ID: 27010029
  • Peterson, MF, et al. (2015) Integrated systems for exosome investigation. Methods. 2015 Oct 1; 87:31-45. PM ID: 25916618
  • Alexander, M, et al. (2015) Exosome-delivered microRNAs modulate the inflammatory response to endotoxin. Nat Commun. 2015 Jun 18; 6:7321. PM ID: 26084661
  • Colluru, VT. (2016) Anti-Tumor DNA vaccination: Optimizing Antigen Expression and Presentation. Thesis. ;. Link: Thesis
  • Hovsepian, T, Saint-Auret, G & Lebot, C. (2017) A Skin Extracellular Signaling Pathway via Intercellular Exosomes Highlighted by Genetic and Epigenetic Analysis Applied to a Natural Ingredient. 29th IFSCC Congress. ;:23-28. Link: 29th IFSCC Congress