Go with the flow for easy exosome isolation using FACS
The CD9 Exo-Flow Capture Kit has all the reagents you need to purify exosomes using FACS—magnetic streptavidin beads, CD9-biotin capture antibody, wash and elution buffers, and reversible Exo-FITC stain (Cat.# EXOFLOW800A-1)—you supply the FACS and the optional magnetic stand (Cat.# EXOFLOW700A-1). Our well-validated CD9-biotin capture antibody and high-quality kit components ensure reliable, reproducible CD9-based exosome purification. And with our larger-than-typical bead size (9.1 μm diameter) exosome capture is highly efficient, assisting with recovery of rare exosome populations.
Note that we also offer reversible Exo-APC (Cat.# Exo-FLOW810A-1) as an alternative stain to Exo-FITC.
A range of well-validated antibodies for reliable and reproducible purification
Large-sized magnetic beads increase the efficiency of exosome capture
Reversible Exo-FITC and Exo-APC stains can be completely removed after FACS
Exosome Elution Buffer simultaneously removes Exo-FITC (or Exo-APC) and elutes exosomes from the magnetic beads for downstream applications such as functional studies
More than just CD9
To facilitate the widest range of studies, SBI built the Exo-Flow system to be highly modular. We offer a range of biotinylated antibodies as well as a Basic Exo-Flow Kit (Cat.# CSFLOWBASICA-1) with no antibody so that you can use your own biotinylated antibodies.
Easily purify exosomes using FACS with the CD9 Exo-Flow Capture Kit
At a glance
Simply (1) couple the CD9-biotin antibody to the magnetic streptavidin beads, (2) use the CD-9-coupled magnetic beads to capture exosomes that have been isolated using either ExoQuick® or ultracentrifugation, (3) wash away unbound exosomes, and then (4) stain with reversible Exo-FITC (excitation and emission wavelengths of 494 nm and 518 nm, respectively).
Your sample is now ready for FACS analysis.
To use the purified exosomes after FACS, add the included Exosome Elution Buffer to simultaneously remove the Exo-FITC stain and elute intact exosomes from the beads.
Supporting Data
See the CD9 Exo-FLOW Capture Kit in action
The CD9 Exo-Flow Capture Kit delivers quantitative, highly selective exosome isolation. Bead flow separation data for exosomes secreted by HEK293 cells and captured using the CD9 Exo-Flow Capture Kit. Plots of forward scatter versus FITC intensity show that in the no exosome control, only 0.5% of particles are FITC-positive (left panel), whereas in the exosome-containing sample, 99.3% of particles are FITC-positive (middle panel). The highly linear binding capacity of the Exo-Flow beads is shown (right panel), with the degree of flow separation shown in the inset graph. Human serum exosomes were isolated from 250 µL serum using ExoQuick® and the exosome pellet resuspended in 500 µL of 1x PBS. Exosome particles were added as two-fold dilutions starting at 50 µL, and then captured using the biotinylated CD9 antibody coupled to Exo-Flow beads. The FITC flow cytometric intensities are then plotted versus the number of exosome particles (determined using NanoSight).
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