Exo-CheckTM Exosome Antibody Array

Streamline your exosome detection with our Exo-CheckTM Exosome Antibody Arrays.

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Exo-Check Exosome Antibody Array

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Exo-Check Exosome Antibody Array

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4 Arrays
EXORAY200A-4
$ 440

Exo-Check Exosome Antibody Array

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Exo-Check Exosome Antibody Array

[variation_id] => 63807 [variation_is_active] => 1 [variation_is_visible] => 1 [weight] => [weight_html] => N/A )
8 Arrays
EXORAY210A-8
$ 835
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Overview

Streamline your exosome detection with Exo-Check Arrays

For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (CD63, CD81, ALIX, FLOT1, ICAM1, EpCam, ANXA5 and TSG101), a GM130 cis-Golgi marker to monitor any cellular contamination in your exosome isolations, a positive control spot derived from human serum exosomes, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.

  • Sensitive—requires as little as 50 µg of exosomal protein for detection
  • Convenient—includes eight antibodies for known exosome markers
  • Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
  • Complete—includes a control for cellular contamination, a background control (blank spot), and a positive control derived from human serum exosomes
  • Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples

A guide to our Exo-Check Exosome Array antibodies

  1 2 3 4 5 6
A Positive control GM130 FLOT-1 ICAM ALIX CD81
B CD63 EpCam ANXA5 TSG101 Blank Positive control

Spot position* ID Notes Link to ExoCarta entry for the human protein
A1 Positive control Positive control for HRP detection (derived from human serum exosomes) NA
A2 GM130 Cis-golgi matrix protein—control for cellular contamination in exosome preparation NA
A3 FLOT1 Flotillin-1 ExoCarta_10211
A4 ICAM1 Intercellular adhesion molecule 1 ExoCarta_3383
A5 ALIX Programmed cell death 6 interacting protein (PDCD6IP) ExoCarta_10015
A6 CD81 Tetraspanin ExoCarta_93185
B1 CD63 Tetraspanin ExoCarta_967
B2 EpCam Epithelial cell adhesion molecule; often found in cancer-derived exosomes ExoCarta_4072
B3 ANXA5 Annexin A5 ExoCarta_308
B4 TSG101 Tumor susceptibility gene 101 ExoCarta_7251
B5 Blank Background control NA
B6 Positive control Positive control for HRP detection (derived from human serum exosomes) NA
*To correctly orient the array, place the notched corner in the upper left-hand position

Supporting Data

Streamlined, semi-quantitative exosome detection

Streamlined, semi-quantitative, serum-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing serum-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from pooled normal human serum using ExoQuick-TC®. The positive control spots should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank spot serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.

Streamlined, semi-quantitative, cell culture-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing cell culture-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 100 µg of exosome proteins isolated from HEK293T cells cultured in SBI’s ExoFBS exosome-depleted media using ExoQuick-TC®. The positive control spots should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank spot serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.