Exo-Check™ Exosome Antibody Array

Streamline your exosome detection with our Exo-Check™ Exosome Antibody Arrays.
  • Sensitive—requires as little as 50 µg of exosomal protein for detection
  • Convenient—includes eight antibodies for known exosome markers
  • Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
  • Complete—includes a control for cellular contamination, a background control (blank spot), and a labeled positive control for HRP detection
  • Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples

Products

Catalog Number Description Size Price Quantity Add to Cart
EXORAY200B-4 Exo-Check Exosome Antibody Array 4 Arrays $498
- +
EXORAY210B-8 Exo-Check Exosome Antibody Array 8 Arrays $944
- +

Overview

Overview

Streamline your exosome detection with Exo-Check Arrays

For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (CD63, CD81, ALIX, FLOT1, ICAM1, EpCam, ANXA5 and TSG101), a GM130 cis-Golgi marker to monitor any cellular contamination in your exosome isolations, a labeled positive control for HRP detection, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.

  • Sensitive—requires as little as 50 µg of exosomal protein for detection
  • Convenient—includes eight antibodies for known exosome markers
  • Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
  • Complete—includes a control for cellular contamination, a background control (blank spot), and a labeled positive control for HRP detection
  • Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples
A guide to our Exo-Check Exosome Array antibodies
123456
ACD63EpCamANXA5TSG101BlankPositive control
BPositive controlGM130FLOT-1ICAMALIXCD81
Spot position*IDNotesLink to ExoCarta entry for the human protein
A1CD63TetraspaninExoCarta_967
A2EpCamEpithelial cell adhesion molecule; often found in cancer-derived exosomesExoCarta_4072
A3ANXA5Annexin A5ExoCarta_308
A4TSG101Tumor susceptibility gene 101ExoCarta_7251
A5BlankBackground controlNA
A6Positive controlPositive control for HRP detection (derived from human serum exosomes)NA
B1Positive controlLabeled positive control for HRP detectionNA
B2GM130Cis-golgi matrix protein—control for cellular contamination in exosome preparationNA
B3FLOT1Flotillin-1ExoCarta_10211
B4ICAM1Intercellular adhesion molecule 1ExoCarta_3383
B5ALIXProgrammed cell death 6 interacting protein (PDCD6IP)ExoCarta_10015
B6CD81TetraspaninExoCarta_93185
*To correctly orient the array, place the notched corner in the upper left-hand position

How It Works

Supporting Data

Supporting Data

Streamlined, semi-quantitative exosome detection

Streamlined, semi-quantitative, serum-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing serum-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from pooled normal human serum using ExoQuick®. The positive control band should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.

Streamlined, semi-quantitative, cell culture-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing cell culture-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from HEK293T cells cultured in SBI’s ExoFBS exosome-depleted media using ExoQuick-TC®. The positive control spots should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.

FAQs

Resources

Citations

  • Villena-Rueda, B, et al. (2023) miR-9-5p is downregulated in serum extracellular vesicles of patients treated with biperiden after traumatic brain injury. Research Square. 2023;. Link: Research Square
  • Honorato-Mauer, J, et al. (2023) Alterations in microRNA of extracellular vesicles associated with major depression, attention-deficit/hyperactivity and anxiety disorders in adolescents. Translational psychiatry. 2023; 13(1):47. PM ID: 36746925
  • Senda, A, et al. (2023) Profiles of lipid, protein and microRNA expression in exosomes derived from intestinal epithelial cells after ischemia-reperfusion injury in a cellular hypoxia model. PloS one. 2023; 18(3):e0283702. PM ID: 36989330
  • Yan, H, et al. (2023) A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs. Nature biomedical engineering. 2023;. PM ID: 37106152
  • Hsu, CC, et al. (2023) Simultaneous Detection of Tumor Derived Exosomal Protein-MicroRNA Pairs with an Exo-PROS Biosensor for Cancer Diagnosis. ACS nano. 2023;. PM ID: 37129374
  • Ahmed, M, et al. (2023) Immune Profile of Exosomes in African American Breast Cancer Patients Is Mediated by Kaiso/THBS1/CD47 Signaling. Cancers. 2023; 15(8):2282. Link: Cancers
  • Dunlop, RA, Banack, SA & Cox, PA. (2023) L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint. RNA biology. 2023; 20(1):140-148. PM ID: 37042019
  • Minic, Z, et al. (2023) Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes. Biomedicines. 2023; 11(4):1076. Link: Biomedicines
  • Mansur, S, et al. (2023) Preparation of Nanoparticle-Loaded Extracellular Vesicles Using Direct Flow Filtration. Pharmaceutics. 2023; 15(5). PM ID: 37242792
  • Meloni, M, et al. (2023) Oligomeric Alpha-Synuclein and STX-1A from Neural-Derived Extracellular Vesicles (NDEVs) as Possible Biomarkers of REM Sleep Behavior Disorder in Parkinson’s Disease: A Preliminary Cohort Study. International journal of molecular sciences. 2023; 24(10). PM ID: 37240185
  • Holzhausen, E, et al. (2023) Human milk EV-miRNAs: a novel biomarker for air pollution exposure during pregnancy. Environmental Research: Health. 2023;. Link: Environmental Research: Health
  • Chu, AJ. (2023) The effects of secreted amyloid precursor protein-alpha on rodent astrocytes and directly reprogrammed human induced neuron-like cells. Thesis. 2023;. Link: Thesis
  • Kang, C, et al. (2023) Harnessing small extracellular vesicles for pro-oxidant delivery: novel approach for drug-sensitive and resistant cancer therapy. Journal of controlled release : official journal of the Controlled Release Society. 2023; 365:286-300. PM ID: 37992875
  • Vitucci, ECM, et al. (2023) Characterizing the extracellular vesicle proteomic landscape of the human airway using in vitro organotypic multi-cellular models. iScience. 2023; 26(11):108162. PM ID: 37920665
  • Carberry, CK, et al. (2023) Extracellular Vesicles altered by a Per- and Polyfluoroalkyl Substance Mixture: In Vitro Dose-Dependent Release, Chemical Content, and MicroRNA Signatures involved in Liver Health. Toxicological sciences : an official journal of the Society of Toxicology. 2023;. PM ID: 37851381
  • Richards, T, et al. (2023) Therapeutic Intervention of Neuroinflammatory Alzheimer Disease Model by Inhibition of Classical Complement Pathway with the Use of Anti-C1r Loaded Exosomes. Research square. 2023;. PM ID: 37886595
  • Zhong, T, et al. (2023) Co-Delivery of Bioengineered Exosomes and Oxygen for Treating Critical Limb Ischemia in Diabetic Mice. ACS nano. 2023; 17(24):25157-25174. PM ID: 38063490
  • Ferroni, L, et al. (2023) Stem cell-derived small extracellular vesicles embedded into methacrylated hyaluronic acid wound dressings accelerate wound repair in a pressure model of diabetic ulcer. Journal of nanobiotechnology. 2023; 21(1):469. PM ID: 38062461
  • Noren Hooten, N, et al. (2022) Association of extracellular vesicle inflammatory proteins and mortality. Scientific reports. 2022; 12(1):14049. PM ID: 35982068
  • Ganesh, V, et al. (2022) Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration. International Journal of Molecular Sciences. 2022; 24(1):466. Link: International Journal of Molecular Sciences

Products

Catalog Number Description Size Price Quantity Add to Cart
EXORAY200B-4 Exo-Check Exosome Antibody Array 4 Arrays $498
- +
EXORAY210B-8 Exo-Check Exosome Antibody Array 8 Arrays $944
- +

Overview

Overview

Streamline your exosome detection with Exo-Check Arrays

For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (CD63, CD81, ALIX, FLOT1, ICAM1, EpCam, ANXA5 and TSG101), a GM130 cis-Golgi marker to monitor any cellular contamination in your exosome isolations, a labeled positive control for HRP detection, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.

  • Sensitive—requires as little as 50 µg of exosomal protein for detection
  • Convenient—includes eight antibodies for known exosome markers
  • Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
  • Complete—includes a control for cellular contamination, a background control (blank spot), and a labeled positive control for HRP detection
  • Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples
A guide to our Exo-Check Exosome Array antibodies
123456
ACD63EpCamANXA5TSG101BlankPositive control
BPositive controlGM130FLOT-1ICAMALIXCD81
Spot position*IDNotesLink to ExoCarta entry for the human protein
A1CD63TetraspaninExoCarta_967
A2EpCamEpithelial cell adhesion molecule; often found in cancer-derived exosomesExoCarta_4072
A3ANXA5Annexin A5ExoCarta_308
A4TSG101Tumor susceptibility gene 101ExoCarta_7251
A5BlankBackground controlNA
A6Positive controlPositive control for HRP detection (derived from human serum exosomes)NA
B1Positive controlLabeled positive control for HRP detectionNA
B2GM130Cis-golgi matrix protein—control for cellular contamination in exosome preparationNA
B3FLOT1Flotillin-1ExoCarta_10211
B4ICAM1Intercellular adhesion molecule 1ExoCarta_3383
B5ALIXProgrammed cell death 6 interacting protein (PDCD6IP)ExoCarta_10015
B6CD81TetraspaninExoCarta_93185
*To correctly orient the array, place the notched corner in the upper left-hand position

How It Works

Supporting Data

Supporting Data

Streamlined, semi-quantitative exosome detection

Streamlined, semi-quantitative, serum-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing serum-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from pooled normal human serum using ExoQuick®. The positive control band should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.

Streamlined, semi-quantitative, cell culture-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing cell culture-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from HEK293T cells cultured in SBI’s ExoFBS exosome-depleted media using ExoQuick-TC®. The positive control spots should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.

FAQs

Citations

  • Villena-Rueda, B, et al. (2023) miR-9-5p is downregulated in serum extracellular vesicles of patients treated with biperiden after traumatic brain injury. Research Square. 2023;. Link: Research Square
  • Honorato-Mauer, J, et al. (2023) Alterations in microRNA of extracellular vesicles associated with major depression, attention-deficit/hyperactivity and anxiety disorders in adolescents. Translational psychiatry. 2023; 13(1):47. PM ID: 36746925
  • Senda, A, et al. (2023) Profiles of lipid, protein and microRNA expression in exosomes derived from intestinal epithelial cells after ischemia-reperfusion injury in a cellular hypoxia model. PloS one. 2023; 18(3):e0283702. PM ID: 36989330
  • Yan, H, et al. (2023) A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs. Nature biomedical engineering. 2023;. PM ID: 37106152
  • Hsu, CC, et al. (2023) Simultaneous Detection of Tumor Derived Exosomal Protein-MicroRNA Pairs with an Exo-PROS Biosensor for Cancer Diagnosis. ACS nano. 2023;. PM ID: 37129374
  • Ahmed, M, et al. (2023) Immune Profile of Exosomes in African American Breast Cancer Patients Is Mediated by Kaiso/THBS1/CD47 Signaling. Cancers. 2023; 15(8):2282. Link: Cancers
  • Dunlop, RA, Banack, SA & Cox, PA. (2023) L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint. RNA biology. 2023; 20(1):140-148. PM ID: 37042019
  • Minic, Z, et al. (2023) Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes. Biomedicines. 2023; 11(4):1076. Link: Biomedicines
  • Mansur, S, et al. (2023) Preparation of Nanoparticle-Loaded Extracellular Vesicles Using Direct Flow Filtration. Pharmaceutics. 2023; 15(5). PM ID: 37242792
  • Meloni, M, et al. (2023) Oligomeric Alpha-Synuclein and STX-1A from Neural-Derived Extracellular Vesicles (NDEVs) as Possible Biomarkers of REM Sleep Behavior Disorder in Parkinson’s Disease: A Preliminary Cohort Study. International journal of molecular sciences. 2023; 24(10). PM ID: 37240185
  • Holzhausen, E, et al. (2023) Human milk EV-miRNAs: a novel biomarker for air pollution exposure during pregnancy. Environmental Research: Health. 2023;. Link: Environmental Research: Health
  • Chu, AJ. (2023) The effects of secreted amyloid precursor protein-alpha on rodent astrocytes and directly reprogrammed human induced neuron-like cells. Thesis. 2023;. Link: Thesis
  • Kang, C, et al. (2023) Harnessing small extracellular vesicles for pro-oxidant delivery: novel approach for drug-sensitive and resistant cancer therapy. Journal of controlled release : official journal of the Controlled Release Society. 2023; 365:286-300. PM ID: 37992875
  • Vitucci, ECM, et al. (2023) Characterizing the extracellular vesicle proteomic landscape of the human airway using in vitro organotypic multi-cellular models. iScience. 2023; 26(11):108162. PM ID: 37920665
  • Carberry, CK, et al. (2023) Extracellular Vesicles altered by a Per- and Polyfluoroalkyl Substance Mixture: In Vitro Dose-Dependent Release, Chemical Content, and MicroRNA Signatures involved in Liver Health. Toxicological sciences : an official journal of the Society of Toxicology. 2023;. PM ID: 37851381
  • Richards, T, et al. (2023) Therapeutic Intervention of Neuroinflammatory Alzheimer Disease Model by Inhibition of Classical Complement Pathway with the Use of Anti-C1r Loaded Exosomes. Research square. 2023;. PM ID: 37886595
  • Zhong, T, et al. (2023) Co-Delivery of Bioengineered Exosomes and Oxygen for Treating Critical Limb Ischemia in Diabetic Mice. ACS nano. 2023; 17(24):25157-25174. PM ID: 38063490
  • Ferroni, L, et al. (2023) Stem cell-derived small extracellular vesicles embedded into methacrylated hyaluronic acid wound dressings accelerate wound repair in a pressure model of diabetic ulcer. Journal of nanobiotechnology. 2023; 21(1):469. PM ID: 38062461
  • Noren Hooten, N, et al. (2022) Association of extracellular vesicle inflammatory proteins and mortality. Scientific reports. 2022; 12(1):14049. PM ID: 35982068
  • Ganesh, V, et al. (2022) Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration. International Journal of Molecular Sciences. 2022; 24(1):466. Link: International Journal of Molecular Sciences