Exo-Check™ Exosome Antibody Array
- Sensitive—requires as little as 50 µg of exosomal protein for detection
- Convenient—includes eight antibodies for known exosome markers
- Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
- Complete—includes a control for cellular contamination, a background control (blank spot), and a labeled positive control for HRP detection
- Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXORAY200B-4 | Exo-Check Exosome Antibody Array | 4 Arrays | $480 |
|
||||
| EXORAY210B-8 | Exo-Check Exosome Antibody Array | 8 Arrays | $910 |
|
||||
Overview
Overview
Streamline your exosome detection with Exo-Check Arrays
For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (CD63, CD81, ALIX, FLOT1, ICAM1, EpCam, ANXA5 and TSG101), a GM130 cis-Golgi marker to monitor any cellular contamination in your exosome isolations, a labeled positive control for HRP detection, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.
- Sensitive—requires as little as 50 µg of exosomal protein for detection
- Convenient—includes eight antibodies for known exosome markers
- Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
- Complete—includes a control for cellular contamination, a background control (blank spot), and a labeled positive control for HRP detection
- Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples
| 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|
| A | Positive control | GM130 | FLOT-1 | ICAM | ALIX | CD81 |
| B | CD63 | EpCam | ANXA5 | TSG101 | Blank | Positive control |
| Spot position* | ID | Notes | Link to ExoCarta entry for the human protein |
|---|---|---|---|
| A1 | Positive control | Labeled positive control for HRP detection | NA |
| A2 | GM130 | Cis-golgi matrix protein—control for cellular contamination in exosome preparation | NA |
| A3 | FLOT1 | Flotillin-1 | ExoCarta_10211 |
| A4 | ICAM1 | Intercellular adhesion molecule 1 | ExoCarta_3383 |
| A5 | ALIX | Programmed cell death 6 interacting protein (PDCD6IP) | ExoCarta_10015 |
| A6 | CD81 | Tetraspanin | ExoCarta_93185 |
| B1 | CD63 | Tetraspanin | ExoCarta_967 |
| B2 | EpCam | Epithelial cell adhesion molecule; often found in cancer-derived exosomes | ExoCarta_4072 |
| B3 | ANXA5 | Annexin A5 | ExoCarta_308 |
| B4 | TSG101 | Tumor susceptibility gene 101 | ExoCarta_7251 |
| B5 | Blank | Background control | NA |
| B6 | Positive control | Positive control for HRP detection (derived from human serum exosomes) | NA |
| *To correctly orient the array, place the notched corner in the upper left-hand position |
How It Works
Supporting Data
Supporting Data
Streamlined, semi-quantitative exosome detection
Sample data showing serum-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from pooled normal human serum using ExoQuick®. The positive control band should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.
Sample data showing cell culture-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from HEK293T cells cultured in SBI’s ExoFBS exosome-depleted media using ExoQuick-TC®. The positive control spots should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.
FAQs
Resources
Related Products
Citations
-
Minic, Z, et al. (2023) Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes. Biomedicines. 2023; 11(4):1076. Link: Biomedicines
-
Dunlop, RA, Banack, SA & Cox, PA. (2023) L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint. RNA biology. 2023; 20(1):140-148. PM ID: 37042019
-
Ahmed, M, et al. (2023) Immune Profile of Exosomes in African American Breast Cancer Patients Is Mediated by Kaiso/THBS1/CD47 Signaling. Cancers. 2023; 15(8):2282. Link: Cancers
-
Hsu, CC, et al. (2023) Simultaneous Detection of Tumor Derived Exosomal Protein-MicroRNA Pairs with an Exo-PROS Biosensor for Cancer Diagnosis. ACS nano. 2023;. PM ID: 37129374
-
Yan, H, et al. (2023) A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs. Nature biomedical engineering. 2023;. PM ID: 37106152
-
Senda, A, et al. (2023) Profiles of lipid, protein and microRNA expression in exosomes derived from intestinal epithelial cells after ischemia-reperfusion injury in a cellular hypoxia model. PloS one. 2023; 18(3):e0283702. PM ID: 36989330
-
Honorato-Mauer, J, et al. (2023) Alterations in microRNA of extracellular vesicles associated with major depression, attention-deficit/hyperactivity and anxiety disorders in adolescents. Translational psychiatry. 2023; 13(1):47. PM ID: 36746925
-
Menon, R, et al. (2022) Differences in cord blood extracellular vesicle cargo in preterm and term births. American journal of reproductive immunology (New York, N.Y. : 1989). 2022;:e13521. PM ID: 35007379
-
Vaka, R, et al. (2022) Direct comparison of different therapeutic cell types susceptibility to inflammatory cytokines associated with COVID-19 acute lung injury. Stem cell research & therapy. 2022; 13(1):20. PM ID: 35033181
-
Hinzman, CP, et al. (2022) An optimized method for the isolation of urinary extracellular vesicles for molecular phenotyping: detection of biomarkers for radiation exposure. Journal of translational medicine. 2022; 20(1):199. PM ID: 35538547
-
Nair, GKG, et al. (2022) Proteomic Insight into the Role of Exosomes in Proliferative Vitreoretinopathy Development. Journal of clinical medicine. 2022; 11(10). PM ID: 35628842
-
Pollalis, D, et al. (2022) Intraocular RGD-Engineered Exosomes and Active Targeting of Choroidal Neovascularization (CNV). Cells. 2022; 11(16). PM ID: 36010651
-
Noren Hooten, N, et al. (2022) Association of extracellular vesicle inflammatory proteins and mortality. Scientific reports. 2022; 12(1):14049. PM ID: 35982068
-
Turner, NJ, et al. (2022) Matrix Bound Nanovesicles have Tissue Specific Characteristics that Suggest a Regulatory Role. Tissue engineering. Part A. 2022;. PM ID: 35946072
-
Rath, ME. (2022) Evaluation of Lymphatic and Glymphatic Associated Extracellular Vesicle Biomarkers for Sport-Related Concussion. Thesis. 2022;. Link: Thesis
-
Singh, AD, et al. (2022) Identifying stable reference genes in polyethene glycol precipitated urinary extracellular vesicles for RT-qPCR-based gene expression studies in renal graft dysfunction patients. Transplant immunology. 2022; 75:101715. PM ID: 36122652
-
Pratt, JT. (2022) Use of small extracellular vesicles for diagnosis of Mycoplasma bovis. Thesis. 2022;. Link: Thesis
-
Crum, RJ, et al. (2022) Biocompatibility and biodistribution of matrix-bound nanovesicles in vitro and in vivo. Acta biomaterialia. 2022;. PM ID: 36423817
-
Ganesh, V, et al. (2022) Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration. International Journal of Molecular Sciences. 2022; 24(1):466. Link: International Journal of Molecular Sciences
-
Mitaki, S, et al. (2021) Proteomic analysis of extracellular vesicles enriched serum associated with future ischemic stroke. Scientific reports. 2021; 11(1):24024. PM ID: 34912031
- See More
Products
| Catalog Number | Description | Size | Price | Quantity | Add to Cart | |||
|---|---|---|---|---|---|---|---|---|
| EXORAY200B-4 | Exo-Check Exosome Antibody Array | 4 Arrays | $480 |
|
||||
| EXORAY210B-8 | Exo-Check Exosome Antibody Array | 8 Arrays | $910 |
|
||||
Overview
Overview
Streamline your exosome detection with Exo-Check Arrays
For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (CD63, CD81, ALIX, FLOT1, ICAM1, EpCam, ANXA5 and TSG101), a GM130 cis-Golgi marker to monitor any cellular contamination in your exosome isolations, a labeled positive control for HRP detection, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.
- Sensitive—requires as little as 50 µg of exosomal protein for detection
- Convenient—includes eight antibodies for known exosome markers
- Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
- Complete—includes a control for cellular contamination, a background control (blank spot), and a labeled positive control for HRP detection
- Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples
| 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|
| A | Positive control | GM130 | FLOT-1 | ICAM | ALIX | CD81 |
| B | CD63 | EpCam | ANXA5 | TSG101 | Blank | Positive control |
| Spot position* | ID | Notes | Link to ExoCarta entry for the human protein |
|---|---|---|---|
| A1 | Positive control | Labeled positive control for HRP detection | NA |
| A2 | GM130 | Cis-golgi matrix protein—control for cellular contamination in exosome preparation | NA |
| A3 | FLOT1 | Flotillin-1 | ExoCarta_10211 |
| A4 | ICAM1 | Intercellular adhesion molecule 1 | ExoCarta_3383 |
| A5 | ALIX | Programmed cell death 6 interacting protein (PDCD6IP) | ExoCarta_10015 |
| A6 | CD81 | Tetraspanin | ExoCarta_93185 |
| B1 | CD63 | Tetraspanin | ExoCarta_967 |
| B2 | EpCam | Epithelial cell adhesion molecule; often found in cancer-derived exosomes | ExoCarta_4072 |
| B3 | ANXA5 | Annexin A5 | ExoCarta_308 |
| B4 | TSG101 | Tumor susceptibility gene 101 | ExoCarta_7251 |
| B5 | Blank | Background control | NA |
| B6 | Positive control | Positive control for HRP detection (derived from human serum exosomes) | NA |
| *To correctly orient the array, place the notched corner in the upper left-hand position |
How It Works
Supporting Data
Supporting Data
Streamlined, semi-quantitative exosome detection
Sample data showing serum-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from pooled normal human serum using ExoQuick®. The positive control band should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.
Sample data showing cell culture-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from HEK293T cells cultured in SBI’s ExoFBS exosome-depleted media using ExoQuick-TC®. The positive control spots should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.
FAQs
Citations
-
Minic, Z, et al. (2023) Lysine Acetylome of Breast Cancer-Derived Small Extracellular Vesicles Reveals Specific Acetylation Patterns for Metabolic Enzymes. Biomedicines. 2023; 11(4):1076. Link: Biomedicines
-
Dunlop, RA, Banack, SA & Cox, PA. (2023) L1CAM immunocapture generates a unique extracellular vesicle population with a reproducible miRNA fingerprint. RNA biology. 2023; 20(1):140-148. PM ID: 37042019
-
Ahmed, M, et al. (2023) Immune Profile of Exosomes in African American Breast Cancer Patients Is Mediated by Kaiso/THBS1/CD47 Signaling. Cancers. 2023; 15(8):2282. Link: Cancers
-
Hsu, CC, et al. (2023) Simultaneous Detection of Tumor Derived Exosomal Protein-MicroRNA Pairs with an Exo-PROS Biosensor for Cancer Diagnosis. ACS nano. 2023;. PM ID: 37129374
-
Yan, H, et al. (2023) A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs. Nature biomedical engineering. 2023;. PM ID: 37106152
-
Senda, A, et al. (2023) Profiles of lipid, protein and microRNA expression in exosomes derived from intestinal epithelial cells after ischemia-reperfusion injury in a cellular hypoxia model. PloS one. 2023; 18(3):e0283702. PM ID: 36989330
-
Honorato-Mauer, J, et al. (2023) Alterations in microRNA of extracellular vesicles associated with major depression, attention-deficit/hyperactivity and anxiety disorders in adolescents. Translational psychiatry. 2023; 13(1):47. PM ID: 36746925
-
Menon, R, et al. (2022) Differences in cord blood extracellular vesicle cargo in preterm and term births. American journal of reproductive immunology (New York, N.Y. : 1989). 2022;:e13521. PM ID: 35007379
-
Vaka, R, et al. (2022) Direct comparison of different therapeutic cell types susceptibility to inflammatory cytokines associated with COVID-19 acute lung injury. Stem cell research & therapy. 2022; 13(1):20. PM ID: 35033181
-
Hinzman, CP, et al. (2022) An optimized method for the isolation of urinary extracellular vesicles for molecular phenotyping: detection of biomarkers for radiation exposure. Journal of translational medicine. 2022; 20(1):199. PM ID: 35538547
-
Nair, GKG, et al. (2022) Proteomic Insight into the Role of Exosomes in Proliferative Vitreoretinopathy Development. Journal of clinical medicine. 2022; 11(10). PM ID: 35628842
-
Pollalis, D, et al. (2022) Intraocular RGD-Engineered Exosomes and Active Targeting of Choroidal Neovascularization (CNV). Cells. 2022; 11(16). PM ID: 36010651
-
Noren Hooten, N, et al. (2022) Association of extracellular vesicle inflammatory proteins and mortality. Scientific reports. 2022; 12(1):14049. PM ID: 35982068
-
Turner, NJ, et al. (2022) Matrix Bound Nanovesicles have Tissue Specific Characteristics that Suggest a Regulatory Role. Tissue engineering. Part A. 2022;. PM ID: 35946072
-
Rath, ME. (2022) Evaluation of Lymphatic and Glymphatic Associated Extracellular Vesicle Biomarkers for Sport-Related Concussion. Thesis. 2022;. Link: Thesis
-
Singh, AD, et al. (2022) Identifying stable reference genes in polyethene glycol precipitated urinary extracellular vesicles for RT-qPCR-based gene expression studies in renal graft dysfunction patients. Transplant immunology. 2022; 75:101715. PM ID: 36122652
-
Pratt, JT. (2022) Use of small extracellular vesicles for diagnosis of Mycoplasma bovis. Thesis. 2022;. Link: Thesis
-
Crum, RJ, et al. (2022) Biocompatibility and biodistribution of matrix-bound nanovesicles in vitro and in vivo. Acta biomaterialia. 2022;. PM ID: 36423817
-
Ganesh, V, et al. (2022) Exosome-Based Cell Homing and Angiogenic Differentiation for Dental Pulp Regeneration. International Journal of Molecular Sciences. 2022; 24(1):466. Link: International Journal of Molecular Sciences
-
Mitaki, S, et al. (2021) Proteomic analysis of extracellular vesicles enriched serum associated with future ischemic stroke. Scientific reports. 2021; 11(1):24024. PM ID: 34912031
- See More