Exo-Check™ Exosome Antibody Array

Streamline your exosome detection with our Exo-Check™ Exosome Antibody Arrays.
  • Sensitive—requires as little as 50 µg of exosomal protein for detection
  • Convenient—includes eight antibodies for known exosome markers
  • Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
  • Complete—includes a control for cellular contamination, a background control (blank spot), and a labeled positive control for HRP detection
  • Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples

Products

Catalog Number Description Size Price Quantity Add to Cart
EXORAY200B-4 Exo-Check Exosome Antibody Array 4 Arrays $470
- +
EXORAY210B-8 Exo-Check Exosome Antibody Array 8 Arrays $892
- +

Overview

Overview

Streamline your exosome detection with Exo-Check Arrays

For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (CD63, CD81, ALIX, FLOT1, ICAM1, EpCam, ANXA5 and TSG101), a GM130 cis-Golgi marker to monitor any cellular contamination in your exosome isolations, a labeled positive control for HRP detection, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.

  • Sensitive—requires as little as 50 µg of exosomal protein for detection
  • Convenient—includes eight antibodies for known exosome markers
  • Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
  • Complete—includes a control for cellular contamination, a background control (blank spot), and a labeled positive control for HRP detection
  • Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples
A guide to our Exo-Check Exosome Array antibodies
 123456
APositive controlGM130FLOT-1ICAMALIXCD81
BCD63EpCamANXA5TSG101BlankPositive control
Spot position*IDNotesLink to ExoCarta entry for the human protein
A1Positive controlLabeled positive control for HRP detectionNA
A2GM130Cis-golgi matrix protein—control for cellular contamination in exosome preparationNA
A3FLOT1Flotillin-1ExoCarta_10211
A4ICAM1Intercellular adhesion molecule 1ExoCarta_3383
A5ALIXProgrammed cell death 6 interacting protein (PDCD6IP)ExoCarta_10015
A6CD81TetraspaninExoCarta_93185
B1CD63TetraspaninExoCarta_967
B2EpCamEpithelial cell adhesion molecule; often found in cancer-derived exosomesExoCarta_4072
B3ANXA5Annexin A5ExoCarta_308
B4TSG101Tumor susceptibility gene 101ExoCarta_7251
B5BlankBackground controlNA
B6Positive controlPositive control for HRP detection (derived from human serum exosomes)NA
*To correctly orient the array, place the notched corner in the upper left-hand position

How It Works

Supporting Data

Supporting Data

Streamlined, semi-quantitative exosome detection

Streamlined, semi-quantitative, serum-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing serum-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from pooled normal human serum using ExoQuick®. The positive control band should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.

Streamlined, semi-quantitative, cell culture-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing cell culture-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from HEK293T cells cultured in SBI’s ExoFBS exosome-depleted media using ExoQuick-TC®. The positive control spots should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.

Resources

Citations

  • Pratt, JT. (2022) Use of small extracellular vesicles for diagnosis of Mycoplasma bovis. Thesis. 1970 Jan 1;. Link: Thesis
  • Singh, AD, et al. (2022) Identifying stable reference genes in polyethene glycol precipitated urinary extracellular vesicles for RT-qPCR-based gene expression studies in renal graft dysfunction patients. Transplant immunology. 1970 Jan 1; 75:101715. PM ID: 36122652
  • Rath, ME. (2022) Evaluation of Lymphatic and Glymphatic Associated Extracellular Vesicle Biomarkers for Sport-Related Concussion. Thesis. 1970 Jan 1;. Link: Thesis
  • Turner, NJ, et al. (2022) Matrix Bound Nanovesicles have Tissue Specific Characteristics that Suggest a Regulatory Role. Tissue engineering. Part A. 1970 Jan 1;. PM ID: 35946072
  • Noren Hooten, N, et al. (2022) Association of extracellular vesicle inflammatory proteins and mortality. Scientific reports. 1970 Jan 1; 12(1):14049. PM ID: 35982068
  • Pollalis, D, et al. (2022) Intraocular RGD-Engineered Exosomes and Active Targeting of Choroidal Neovascularization (CNV). Cells. 1970 Jan 1; 11(16). PM ID: 36010651
  • Nair, GKG, et al. (2022) Proteomic Insight into the Role of Exosomes in Proliferative Vitreoretinopathy Development. Journal of clinical medicine. 1970 Jan 1; 11(10). PM ID: 35628842
  • Hinzman, CP, et al. (2022) An optimized method for the isolation of urinary extracellular vesicles for molecular phenotyping: detection of biomarkers for radiation exposure. Journal of translational medicine. 1970 Jan 1; 20(1):199. PM ID: 35538547
  • Menon, R, et al. (2022) Differences in cord blood extracellular vesicle cargo in preterm and term births. American journal of reproductive immunology (New York, N.Y. : 1989). 1970 Jan 1;:e13521. PM ID: 35007379
  • Vaka, R, et al. (2022) Direct comparison of different therapeutic cell types susceptibility to inflammatory cytokines associated with COVID-19 acute lung injury. Stem cell research & therapy. 1970 Jan 1; 13(1):20. PM ID: 35033181
  • Mitaki, S, et al. (2021) Proteomic analysis of extracellular vesicles enriched serum associated with future ischemic stroke. Scientific reports. 1970 Jan 1; 11(1):24024. PM ID: 34912031
  • Morrissey, SM, et al. (2021) Tumor-derived exosomes drive immunosuppressive macrophages in a pre-metastatic niche through glycolytic dominant metabolic reprogramming. Cell metabolism. 1970 Jan 1;. PM ID: 34559989
  • Radnaa, E, et al. (2021) Extracellular Vesicles from Maternal Uterine Cells Exposed to Risk Factors cause Fetal Inflammatory Response. Research Square. 1970 Jan 1;. Link: Research Square
  • Liu, HY, et al. (2021) Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays. Advanced materials (Deerfield Beach, Fla.). 1970 Jan 1;:e2008493. PM ID: 34309083
  • Rodrigues, FCR. (2019) Avaliação da qualidade das amostras de biópsias líquidas de pacientes com tumores urológicos, colhidas e armazenadas no Biobanco-iMM, CAML. Thesis. 1970 Jan 1;. Link: Thesis
  • CÓ, AC, et al. (2019) Epidemiological Profile of High-Risk HPV in Head and Neck Cancer. dl.uswr.ac.ir. 1970 Jan 1; 126(3). Link: dl.uswr.ac.ir
  • DOURADO, MD, et al. (2019) Extracellular Vesicles Derived From CAFs Induced OSCC Cell Migration, Invasion, and Spreading in Vitro. dl.uswr.ac.ir. 1970 Jan 1; 126(3). Link: dl.uswr.ac.ir
  • GONÇALVES, RN, et al. (2019) Analysis of Clinical Features, Treatment, and Quality of Life in Patients with Oral Squamous Cell Carcinoma. dl.uswr.ac.ir. 1970 Jan 1; 126(3). Link: dl.uswr.ac.ir
  • Arunsan, P, et al. (2019) Liver fluke granulin promotes exosome-mediated crosstalk and cellular microenvironment conducive to cholangiocarcinoma. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Arunsan, P, et al. (2019) Exosome-mediated crosstalk stimulated by liver fluke granulin promotes a microenvironment conducive to cholangiocarcinoma. bioRxiv. 1970 Jan 1;. Link: bioRxiv

Products

Catalog Number Description Size Price Quantity Add to Cart
EXORAY200B-4 Exo-Check Exosome Antibody Array 4 Arrays $470
- +
EXORAY210B-8 Exo-Check Exosome Antibody Array 8 Arrays $892
- +

Overview

Overview

Streamline your exosome detection with Exo-Check Arrays

For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (CD63, CD81, ALIX, FLOT1, ICAM1, EpCam, ANXA5 and TSG101), a GM130 cis-Golgi marker to monitor any cellular contamination in your exosome isolations, a labeled positive control for HRP detection, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.

  • Sensitive—requires as little as 50 µg of exosomal protein for detection
  • Convenient—includes eight antibodies for known exosome markers
  • Flexible—compatible with most exosome isolation methods, include the ExoQuick® family of reagents and ultracentrifugation
  • Complete—includes a control for cellular contamination, a background control (blank spot), and a labeled positive control for HRP detection
  • Semi-quantitative—can be used to evaluate relative abundance of certain exosome markers from a given set of samples
A guide to our Exo-Check Exosome Array antibodies
 123456
APositive controlGM130FLOT-1ICAMALIXCD81
BCD63EpCamANXA5TSG101BlankPositive control
Spot position*IDNotesLink to ExoCarta entry for the human protein
A1Positive controlLabeled positive control for HRP detectionNA
A2GM130Cis-golgi matrix protein—control for cellular contamination in exosome preparationNA
A3FLOT1Flotillin-1ExoCarta_10211
A4ICAM1Intercellular adhesion molecule 1ExoCarta_3383
A5ALIXProgrammed cell death 6 interacting protein (PDCD6IP)ExoCarta_10015
A6CD81TetraspaninExoCarta_93185
B1CD63TetraspaninExoCarta_967
B2EpCamEpithelial cell adhesion molecule; often found in cancer-derived exosomesExoCarta_4072
B3ANXA5Annexin A5ExoCarta_308
B4TSG101Tumor susceptibility gene 101ExoCarta_7251
B5BlankBackground controlNA
B6Positive controlPositive control for HRP detection (derived from human serum exosomes)NA
*To correctly orient the array, place the notched corner in the upper left-hand position

How It Works

Supporting Data

Supporting Data

Streamlined, semi-quantitative exosome detection

Streamlined, semi-quantitative, serum-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing serum-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from pooled normal human serum using ExoQuick®. The positive control band should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.

Streamlined, semi-quantitative, cell culture-derived exosome detection with Exo-Check Exosome Antibody Arrays

Sample data showing cell culture-derived exosome detection with an Exo-Check Exosome Antibody Array. The array was exposed to 50 µg of exosome proteins isolated from HEK293T cells cultured in SBI’s ExoFBS exosome-depleted media using ExoQuick-TC®. The positive control spots should provide strong signals to indicate that all of the detection reagents are working properly. The various exosome antibody spots will provide varying levels of signal, depending upon the source of the isolated exosomes. The blank band serves as a background control and should not have any signal. The GM130 control will only show signal above background if there is cellular contamination in your exosome preparations.

Citations

  • Pratt, JT. (2022) Use of small extracellular vesicles for diagnosis of Mycoplasma bovis. Thesis. 1970 Jan 1;. Link: Thesis
  • Singh, AD, et al. (2022) Identifying stable reference genes in polyethene glycol precipitated urinary extracellular vesicles for RT-qPCR-based gene expression studies in renal graft dysfunction patients. Transplant immunology. 1970 Jan 1; 75:101715. PM ID: 36122652
  • Rath, ME. (2022) Evaluation of Lymphatic and Glymphatic Associated Extracellular Vesicle Biomarkers for Sport-Related Concussion. Thesis. 1970 Jan 1;. Link: Thesis
  • Turner, NJ, et al. (2022) Matrix Bound Nanovesicles have Tissue Specific Characteristics that Suggest a Regulatory Role. Tissue engineering. Part A. 1970 Jan 1;. PM ID: 35946072
  • Noren Hooten, N, et al. (2022) Association of extracellular vesicle inflammatory proteins and mortality. Scientific reports. 1970 Jan 1; 12(1):14049. PM ID: 35982068
  • Pollalis, D, et al. (2022) Intraocular RGD-Engineered Exosomes and Active Targeting of Choroidal Neovascularization (CNV). Cells. 1970 Jan 1; 11(16). PM ID: 36010651
  • Nair, GKG, et al. (2022) Proteomic Insight into the Role of Exosomes in Proliferative Vitreoretinopathy Development. Journal of clinical medicine. 1970 Jan 1; 11(10). PM ID: 35628842
  • Hinzman, CP, et al. (2022) An optimized method for the isolation of urinary extracellular vesicles for molecular phenotyping: detection of biomarkers for radiation exposure. Journal of translational medicine. 1970 Jan 1; 20(1):199. PM ID: 35538547
  • Menon, R, et al. (2022) Differences in cord blood extracellular vesicle cargo in preterm and term births. American journal of reproductive immunology (New York, N.Y. : 1989). 1970 Jan 1;:e13521. PM ID: 35007379
  • Vaka, R, et al. (2022) Direct comparison of different therapeutic cell types susceptibility to inflammatory cytokines associated with COVID-19 acute lung injury. Stem cell research & therapy. 1970 Jan 1; 13(1):20. PM ID: 35033181
  • Mitaki, S, et al. (2021) Proteomic analysis of extracellular vesicles enriched serum associated with future ischemic stroke. Scientific reports. 1970 Jan 1; 11(1):24024. PM ID: 34912031
  • Morrissey, SM, et al. (2021) Tumor-derived exosomes drive immunosuppressive macrophages in a pre-metastatic niche through glycolytic dominant metabolic reprogramming. Cell metabolism. 1970 Jan 1;. PM ID: 34559989
  • Radnaa, E, et al. (2021) Extracellular Vesicles from Maternal Uterine Cells Exposed to Risk Factors cause Fetal Inflammatory Response. Research Square. 1970 Jan 1;. Link: Research Square
  • Liu, HY, et al. (2021) Rapid Capture of Cancer Extracellular Vesicles by Lipid Patch Microarrays. Advanced materials (Deerfield Beach, Fla.). 1970 Jan 1;:e2008493. PM ID: 34309083
  • Rodrigues, FCR. (2019) Avaliação da qualidade das amostras de biópsias líquidas de pacientes com tumores urológicos, colhidas e armazenadas no Biobanco-iMM, CAML. Thesis. 1970 Jan 1;. Link: Thesis
  • CÓ, AC, et al. (2019) Epidemiological Profile of High-Risk HPV in Head and Neck Cancer. dl.uswr.ac.ir. 1970 Jan 1; 126(3). Link: dl.uswr.ac.ir
  • DOURADO, MD, et al. (2019) Extracellular Vesicles Derived From CAFs Induced OSCC Cell Migration, Invasion, and Spreading in Vitro. dl.uswr.ac.ir. 1970 Jan 1; 126(3). Link: dl.uswr.ac.ir
  • GONÇALVES, RN, et al. (2019) Analysis of Clinical Features, Treatment, and Quality of Life in Patients with Oral Squamous Cell Carcinoma. dl.uswr.ac.ir. 1970 Jan 1; 126(3). Link: dl.uswr.ac.ir
  • Arunsan, P, et al. (2019) Liver fluke granulin promotes exosome-mediated crosstalk and cellular microenvironment conducive to cholangiocarcinoma. bioRxiv. 1970 Jan 1;. Link: bioRxiv
  • Arunsan, P, et al. (2019) Exosome-mediated crosstalk stimulated by liver fluke granulin promotes a microenvironment conducive to cholangiocarcinoma. bioRxiv. 1970 Jan 1;. Link: bioRxiv