Overview
Efficiently generate mRNAs
When you want instant expression after transfection into cells, deliver your gene-of-interest as an mRNA made with SBI’s mRNAExpress™ mRNA Synthesis Kit. Designed to generate in vitro transcripts for transfection of mammalian cells, micro-injection into oocytes, in vitro translation, and other related applications, this high-yield kit can produce 20—40 µg of high-quality mRNAs in one standard reaction, and comes with a number of performance-enhancing features:
- Leverages the robust T7 RNA Polymerase
- Incorporates an anti-reverse cap analog (ARCA) into the transcript to increase translation efficiency
- Reduces host cell immune response and enhances stability by incorporating modified nucleotides (5-Methylcytidine-5'-Triphosphate and Pseudouridine-5'-Triphosphate) and a poly-A tail
- Degrades the DNA template after RNA synthesis with the included DNase
- Removes the 5' triphosphates at the end of the RNA with the included phosphatase to further reduce innate immune responses in mammalian cells
- Employs a robust clean-up spin column system that delivers high yields of mRNAs that are ready for most downstream applications
The Kit includes the following reagents that are critical in vitro transcription:
- pMRNAXP mRNAExpress Vector so you can clone and then transcribe your cDNA-of-interest
- Sequencing primers to confirm the identity of the cDNA insert cloned into the pMRNAXP Vector
- Tail PCR primer mix to add a poly-A tail to the DNA template
- In vitro transcription reagents—T7 RNA polymerase, in vitro transcription buffer, NTP/Cap mix, RNase-free DNase, phosphatase buffer and RNase-free phosphatase
- Clean-up spin column system, including binding, wash, and elution buffers, collection tubes, and collection tubes with filter cartridges
