SeraMir Spike-in RNA Control and qPCR Assay

A great addition to your SeraMir qPCR profiling studies, the SeraMir Spike-in RNA Control and qPCR Assay controls for exoRNA recovery, tailing, and cDNA synthesis.

Products

Catalog Number Description Size Price Quantity Add to Cart
RA805A-1 SeraMir Control spike-in Small RNA and qPCR assay 100 Assays $174.00
- +
Contact Us

Overview

Overview

Get extra assurance for your SeraMir qPCR profiling studies To help researchers with their SeraMir qPCR profiling studies, SBI offers the SeraMir Spike-in RNA Control and qPCR Assay. This product gives researchers the ability to control for exoRNA recovery, tailing, and cDNA synthesis by spiking in known quantities of a control RNA during exoRNA isolation—can be used directly on the 384-well SeraMir profiling plates or in an individual control spike-in qPCR assay. Choose the SeraMir Kit that’s right for you
Cat. #NameKit includes
ExoQuick or ExoQuick-TCSeraMir RNA columns and reagentsSeraMir cDNA synthesis and amplification reagents384-well plate miRNAs for human, mouse, or rat
RA800A-1Complete SeraMir Exosome RNA Amplification Kit 
RA800TC-1Complete SeraMir Exosome RNA Amplification Kit for Media and Urine 
RA806A-1SeraMir Exosome RNA Purification Kit  
RA806TC-1SeraMir Exosome RNA Purification Kit for Media and Urine  
RA808A-1SeraMir Exosome RNA Purification Column Kit   
RA820A-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA820TC-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA821A-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA821TC-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA822A-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA822TC-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine

How It Works

How It Works

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis

If 5 µL of SeraMir Spike-in RNA was used during exoRNA isolation, then you should expect to observe a Ct of about 15 to 20.

Supporting Data

Resources

Citations

  • Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. ;. Link: Conference
  • Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. ;. Link: Abstract
  • Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017 Apr 15;. PM ID: 28411393
  • Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017 Jan 1; 25(1):102-110. PM ID: 27883272
  • Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017 Apr 1; 31(9):1277-1295. PM ID: 28447547
  • Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017 Feb 1; 15:163-172. PM ID: 27974246
  • Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017 Jun 13;. PM ID: 28623135
  • Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017 Jun 4;. PM ID: 28580875
  • Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017 Jun 1;:95-99. PM ID: 28643924
  • Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017 May 1; 4(2):112-121. PM ID: 28451447
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product
  • Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. ; 7(4). Link: bio-protocol
  • Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017 Jan 1; 21(1):55-66. PM ID: 28271982
  • Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017 Jun 27; 8(26):42712-42727. PM ID: 28514744
  • Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017 Apr 5;. PM ID: 28157838
  • Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016 Feb 1; 429:39-49. PM ID: 26723490
  • Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. ; 5(1):1–94. Link: Liver Cancer
  • Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016 Mar 1; 5:30924. PM ID: 26928673
  • Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016 Mar 2; 11(3):e0150154. PM ID: 26930277
  • Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016 Jul 20; 39:427-432. Link: 中华检验医学杂志

Products

Catalog Number Description Size Price Quantity Add to Cart
RA805A-1 SeraMir Control spike-in Small RNA and qPCR assay 100 Assays $174.00
- +
Contact Us

Overview

Overview

Get extra assurance for your SeraMir qPCR profiling studies To help researchers with their SeraMir qPCR profiling studies, SBI offers the SeraMir Spike-in RNA Control and qPCR Assay. This product gives researchers the ability to control for exoRNA recovery, tailing, and cDNA synthesis by spiking in known quantities of a control RNA during exoRNA isolation—can be used directly on the 384-well SeraMir profiling plates or in an individual control spike-in qPCR assay. Choose the SeraMir Kit that’s right for you
Cat. #NameKit includes
ExoQuick or ExoQuick-TCSeraMir RNA columns and reagentsSeraMir cDNA synthesis and amplification reagents384-well plate miRNAs for human, mouse, or rat
RA800A-1Complete SeraMir Exosome RNA Amplification Kit 
RA800TC-1Complete SeraMir Exosome RNA Amplification Kit for Media and Urine 
RA806A-1SeraMir Exosome RNA Purification Kit  
RA806TC-1SeraMir Exosome RNA Purification Kit for Media and Urine  
RA808A-1SeraMir Exosome RNA Purification Column Kit   
RA820A-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA820TC-1Human Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA821A-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA821TC-1Mouse Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine
RA822A-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit
RA822TC-1Rat Complete SeraMir Exosome RNA Amplification and Profiling Kit for Media and Urine

How It Works

How It Works

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis.

Use the SeraMir spike-in RNA control in a qPCR assay to control for exoRNA recovery, tailing, and cDNA synthesis

If 5 µL of SeraMir Spike-in RNA was used during exoRNA isolation, then you should expect to observe a Ct of about 15 to 20.

Supporting Data

Citations

  • Peng, Y, Huleihel, L & Cardenes, N. (2017) Different MiroRNA Expression In MSC-Derived Exosomes: IPF Patients And Age-Matched Normal Individuals. Conference. ;. Link: Conference
  • Sueta, A, et al. (2017) Abstract P1-02-13: Differential expression of exosomal miRNAs between breast cancer patients with recurrence and no-recurrence. Abstract. ;. Link: Abstract
  • Selmaj, I, et al. (2017) Global Exosome Transcriptome Profiling Reveals Biomarkers for Multiple Sclerosis. Ann. Neurol.. 2017 Apr 15;. PM ID: 28411393
  • Hubal, MJ, et al. (2017) Circulating adipocyte-derived exosomal MicroRNAs associated with decreased insulin resistance after gastric bypass. Obesity (Silver Spring). 2017 Jan 1; 25(1):102-110. PM ID: 27883272
  • Faust, A, et al. (2017) Urinary bladder extracellular matrix hydrogels and matrix-bound vesicles differentially regulate central nervous system neuron viability and axon growth and branching. J Biomater Appl. 2017 Apr 1; 31(9):1277-1295. PM ID: 28447547
  • Tsukita, S, et al. (2017) MicroRNAs 106b and 222 Improve Hyperglycemia in a Mouse Model of Insulin-Deficient Diabetes via Pancreatic β-Cell Proliferation. EBioMedicine. 2017 Feb 1; 15:163-172. PM ID: 27974246
  • Zhang, R, et al. (2017) Serum long non coding RNA MALAT-1 protected by exosomes is up-regulated and promotes cell proliferation and migration in non-small cell lung cancer. Biochem. Biophys. Res. Commun.. 2017 Jun 13;. PM ID: 28623135
  • Huleihel, L, et al. (2017) Matrix bound nanovesicles recapitulate extracellular matrix effects on macrophage phenotype. Tissue Eng Part A. 2017 Jun 4;. PM ID: 28580875
  • Holliday, LS, et al. (2017) Exosomes: novel regulators of bone remodelling and potential therapeutic agents for orthodontics. Orthod Craniofac Res. 2017 Jun 1;:95-99. PM ID: 28643924
  • Koyama, S, et al. (2017) Dynamic changes of serum microRNA-122-5p through therapeutic courses indicates amelioration of acute liver injury accompanied by acute cardiac decompensation. ESC Heart Fail. 2017 May 1; 4(2):112-121. PM ID: 28451447
  • Protocol, IIEI. (2017) List of Components. Product. ;. Link: Product
  • Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles. bio-protocol. ; 7(4). Link: bio-protocol
  • Nakano, T, et al. (2017) Hepatic miR-301a as a Liver Transplant Rejection Biomarker? And Its Role for Interleukin-6 Production in Hepatocytes. OMICS. 2017 Jan 1; 21(1):55-66. PM ID: 28271982
  • Zhou, X, et al. (2017) Characterization of mouse serum exosomal small RNA content: The origins and their roles in modulating inflammatory response. Oncotarget. 2017 Jun 27; 8(26):42712-42727. PM ID: 28514744
  • Val, S, et al. (2017) Purification and characterization of microRNAs within middle ear fluid exosomes: implication in otitis media pathophysiology. Pediatr. Res.. 2017 Apr 5;. PM ID: 28157838
  • Crossland, RE, et al. (2016) Evaluation of optimal extracellular vesicle small RNA isolation and qRT-PCR normalisation for serum and urine. J. Immunol. Methods. 2016 Feb 1; 429:39-49. PM ID: 26723490
  • Kudo, M. (2016) Speaker’s Lecture Abstracts. Liver Cancer. ; 5(1):1–94. Link: Liver Cancer
  • Clayton, A, et al. (2016) The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts: 15 December 2015, Hadyn Ellis Building, Cardiff University.. J Extracell Vesicles. 2016 Mar 1; 5:30924. PM ID: 26928673
  • Delić, D, et al. (2016) Urinary Exosomal miRNA Signature in Type II Diabetic Nephropathy Patients. PLoS ONE. 2016 Mar 2; 11(3):e0150154. PM ID: 26930277
  • Huang, Y, et al. (2016) 血清中外泌体及外泌体 RNA 提取方法的比较. 中华检验医学杂志. 2016 Jul 20; 39:427-432. Link: 中华检验医学杂志