PureFection™ Transfection Reagent

Deliver more nucleic acids—plasmids, siRNAs, etc.—to cells than the leading lipid-based transfection reagent for effective and reproducible transfection
  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

Products

Catalog Number Description Size Price Quantity Add to Cart
LV750A-1 PureFection Transfection Reagent 1 mL $314
- +
LV750A-5 PureFection Transfection Reagent 5 mL $1335
- +

Overview

Overview

Increase your transfection efficiencies

With SBI’s PureFection™ Transfection Reagent, you can deliver more nucleic acid—plasmids, siRNAs, etc.—than the leading lipid-based transfection reagent for effective, efficient, and reproducible transfections.

The easy-to-use protocol consists of a rapid, one-step, 15-minute incubation with the plasmid, small RNA, or other nucleic acid you’d like to transfect. Once the incubation is done, simply add directly to target cells—no media changes are required as PureFection works in the presence of antibiotics and serum.

The fast PureFection protocol makes it well-suited for high-throughput transfection projects.

  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

How It Works

How It Works

A fast and effective method for increasing transfection efficiencies

The PureFection Transfection Reagent uses a fast, 15-minute protocol

Supporting Data

Supporting Data

PureFection delivers higher transfection efficiencies than the leading lipid-based reagent

PureFection delivers higher transfection efficiencies than the leading lipid-based reagentPureFection delivers higher transfection efficiencies than the leading lipid-based reagent

FAQs

Resources

Citations

  • Lin, KH, et al. (2024) Diallyl trisulfide (DATS) protects cardiac cells against advanced glycation end-product-induced apoptosis by enhancing FoxO3A-dependent upregulation of miRNA-210. The Journal of nutritional biochemistry. 2024; 125:109567. PM ID: 38185348
  • Wu, H, et al. (2023) Inhibition of microRNA-122 alleviates pyroptosis by targeting dual-specificity phosphatase 4 in myocardial ischemia/reperfusion injury. Heliyon. 2023; 9(7):e18238. Link: Heliyon
  • Middleton, RC, et al. (2023) Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth. Scientific reports. 2023; 13(1):11829. PM ID: 37481602
  • Middleton, R, et al. (2023) Newt-derived extracellular vesicles promote mammalian nerve growth. Research Square. 2023;. Link: Research Square
  • Aftab, F, et al. (2023) An intrinsic purine metabolite AICAR blocks lung tumour growth by targeting oncoprotein mucin 1. British journal of cancer. 2023;. PM ID: 36810913
  • Ichinohe, N, et al. (2023) CINC-2 and miR-199a-5p in exosomes secreted by transplanted Thy1+ cells activate hepatocytic progenitor cell growth in rat liver regeneration. Research Square. 2023;. Link: Research Square
  • Mitchell, W, et al. (2023) Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation. bioRxiv : the preprint server for biology. 2023;. PM ID: 37425825
  • Kumar, A, et al. (2023) SHP-1 phosphatase acts as a co-activator of PCK1 transcription to control gluconeogenesis. The Journal of biological chemistry. 2023;:105164. PM ID: 37595871
  • Wen, SY, et al. (2023) Doxorubicin induced ROS-dependent HIF1α activation mediates blockage of IGF1R survival signaling by IGFBP3 promotes cardiac apoptosis. Aging. 2023; 15(1):164-178. PM ID: 36602546
  • Tsvetankova, R, et al. (2023) Combined microRNA-141 Rescue and MAPK1 Silencing as Putative Strategy to Support Chemotherapy in Translational Stage towards Metastatic Castration-resistant Prostate Cancer – an In Vitro Model Study. Proceedings of the Bulgarian Academy of Sciences. 2023; 76(8):1286-1296. Link: Proceedings of the Bulgarian Academy of Sciences
  • Buri, M, et al. (2023) NK cells shape the clonal evolution of B-ALL cells by IFN-γ production. bioRxiv. 2023;. Link: bioRxiv
  • Barik, P, et al. (2023) Rewiring of IGF1 secretion and enhanced IGF1R signaling induced by co-chaperone carboxyl-terminus of Hsp70 interacting protein in adipose-derived stem cells provide augmented cardioprotection in aging-hypertensive rats. Aging. 2023; 15(23):14019-14038. PM ID: 38085649
  • Seigner, J, et al. (2023) Solving the mystery of the FMC63-CD19 affinity. Scientific reports. 2023; 13(1):23024. PM ID: 38155191
  • Tsvetankova, R, et al. (2023) Restoring mitophagy in prostate cancer cells: the role of miR-141 rescue in counteracting MAPK1/ERK2-dependent autophagy suppression. Biotechnology & Biotechnological Equipment. 2023; 37(1). Link: Biotechnology & Biotechnological Equipment
  • Savill, KMZ, et al. (2022) Distinct resistance mechanisms arise to allosteric vs. ATP-competitive AKT inhibitors. Nature communications. 2022; 13(1):2057. PM ID: 35440108
  • Mai, A, et al. (2022) Thymoquinone induces apoptosis in temozolomide-resistant glioblastoma cells via the p38 mitogen-activated protein kinase signaling pathway. Environmental toxicology. 2022;. PM ID: 36176197
  • Saggu, S, et al. (2022) Activation of a novel α2AAR-spinophilin-cofilin axis determines the effect of α2 adrenergic drugs on fear memory reconsolidation. Molecular psychiatry. 2022;:1-13. PM ID: 36357671
  • Osborne, OM, Kowalczyk, JM & Pierre Louis, KD. (2022) Brain endothelium-derived extracellular vesicles containing amyloid-beta induce mitochondrial alterations in neural progenitor cells. Extracellular Vesicles and Circulating Nucleic Acids. 2022; 3(4):340-361. Link: Extracellular Vesicles and Circulating Nucleic Acids
  • Stofner, S & Konig, EM. (2022) The role of serine/threonine kinases STK38 and STK38L in human natural killer cells. Thesis. 2022;. Link: Thesis
  • Zhang, WC, et al. (2022) MicroRNA-21 guide and passenger strand regulation of adenylosuccinate lyase-mediated purine metabolism promotes transition to an EGFR-TKI-tolerant persister state. Cancer gene therapy. 2022;. PM ID: 35840668

Products

Catalog Number Description Size Price Quantity Add to Cart
LV750A-1 PureFection Transfection Reagent 1 mL $314
- +
LV750A-5 PureFection Transfection Reagent 5 mL $1335
- +

Overview

Overview

Increase your transfection efficiencies

With SBI’s PureFection™ Transfection Reagent, you can deliver more nucleic acid—plasmids, siRNAs, etc.—than the leading lipid-based transfection reagent for effective, efficient, and reproducible transfections.

The easy-to-use protocol consists of a rapid, one-step, 15-minute incubation with the plasmid, small RNA, or other nucleic acid you’d like to transfect. Once the incubation is done, simply add directly to target cells—no media changes are required as PureFection works in the presence of antibiotics and serum.

The fast PureFection protocol makes it well-suited for high-throughput transfection projects.

  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

How It Works

How It Works

A fast and effective method for increasing transfection efficiencies

The PureFection Transfection Reagent uses a fast, 15-minute protocol

Supporting Data

Supporting Data

PureFection delivers higher transfection efficiencies than the leading lipid-based reagent

PureFection delivers higher transfection efficiencies than the leading lipid-based reagentPureFection delivers higher transfection efficiencies than the leading lipid-based reagent

FAQs

Citations

  • Lin, KH, et al. (2024) Diallyl trisulfide (DATS) protects cardiac cells against advanced glycation end-product-induced apoptosis by enhancing FoxO3A-dependent upregulation of miRNA-210. The Journal of nutritional biochemistry. 2024; 125:109567. PM ID: 38185348
  • Wu, H, et al. (2023) Inhibition of microRNA-122 alleviates pyroptosis by targeting dual-specificity phosphatase 4 in myocardial ischemia/reperfusion injury. Heliyon. 2023; 9(7):e18238. Link: Heliyon
  • Middleton, RC, et al. (2023) Newt A1 cell-derived extracellular vesicles promote mammalian nerve growth. Scientific reports. 2023; 13(1):11829. PM ID: 37481602
  • Middleton, R, et al. (2023) Newt-derived extracellular vesicles promote mammalian nerve growth. Research Square. 2023;. Link: Research Square
  • Aftab, F, et al. (2023) An intrinsic purine metabolite AICAR blocks lung tumour growth by targeting oncoprotein mucin 1. British journal of cancer. 2023;. PM ID: 36810913
  • Ichinohe, N, et al. (2023) CINC-2 and miR-199a-5p in exosomes secreted by transplanted Thy1+ cells activate hepatocytic progenitor cell growth in rat liver regeneration. Research Square. 2023;. Link: Research Square
  • Mitchell, W, et al. (2023) Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenation. bioRxiv : the preprint server for biology. 2023;. PM ID: 37425825
  • Kumar, A, et al. (2023) SHP-1 phosphatase acts as a co-activator of PCK1 transcription to control gluconeogenesis. The Journal of biological chemistry. 2023;:105164. PM ID: 37595871
  • Wen, SY, et al. (2023) Doxorubicin induced ROS-dependent HIF1α activation mediates blockage of IGF1R survival signaling by IGFBP3 promotes cardiac apoptosis. Aging. 2023; 15(1):164-178. PM ID: 36602546
  • Tsvetankova, R, et al. (2023) Combined microRNA-141 Rescue and MAPK1 Silencing as Putative Strategy to Support Chemotherapy in Translational Stage towards Metastatic Castration-resistant Prostate Cancer – an In Vitro Model Study. Proceedings of the Bulgarian Academy of Sciences. 2023; 76(8):1286-1296. Link: Proceedings of the Bulgarian Academy of Sciences
  • Buri, M, et al. (2023) NK cells shape the clonal evolution of B-ALL cells by IFN-γ production. bioRxiv. 2023;. Link: bioRxiv
  • Barik, P, et al. (2023) Rewiring of IGF1 secretion and enhanced IGF1R signaling induced by co-chaperone carboxyl-terminus of Hsp70 interacting protein in adipose-derived stem cells provide augmented cardioprotection in aging-hypertensive rats. Aging. 2023; 15(23):14019-14038. PM ID: 38085649
  • Seigner, J, et al. (2023) Solving the mystery of the FMC63-CD19 affinity. Scientific reports. 2023; 13(1):23024. PM ID: 38155191
  • Tsvetankova, R, et al. (2023) Restoring mitophagy in prostate cancer cells: the role of miR-141 rescue in counteracting MAPK1/ERK2-dependent autophagy suppression. Biotechnology & Biotechnological Equipment. 2023; 37(1). Link: Biotechnology & Biotechnological Equipment
  • Savill, KMZ, et al. (2022) Distinct resistance mechanisms arise to allosteric vs. ATP-competitive AKT inhibitors. Nature communications. 2022; 13(1):2057. PM ID: 35440108
  • Mai, A, et al. (2022) Thymoquinone induces apoptosis in temozolomide-resistant glioblastoma cells via the p38 mitogen-activated protein kinase signaling pathway. Environmental toxicology. 2022;. PM ID: 36176197
  • Saggu, S, et al. (2022) Activation of a novel α2AAR-spinophilin-cofilin axis determines the effect of α2 adrenergic drugs on fear memory reconsolidation. Molecular psychiatry. 2022;:1-13. PM ID: 36357671
  • Osborne, OM, Kowalczyk, JM & Pierre Louis, KD. (2022) Brain endothelium-derived extracellular vesicles containing amyloid-beta induce mitochondrial alterations in neural progenitor cells. Extracellular Vesicles and Circulating Nucleic Acids. 2022; 3(4):340-361. Link: Extracellular Vesicles and Circulating Nucleic Acids
  • Stofner, S & Konig, EM. (2022) The role of serine/threonine kinases STK38 and STK38L in human natural killer cells. Thesis. 2022;. Link: Thesis
  • Zhang, WC, et al. (2022) MicroRNA-21 guide and passenger strand regulation of adenylosuccinate lyase-mediated purine metabolism promotes transition to an EGFR-TKI-tolerant persister state. Cancer gene therapy. 2022;. PM ID: 35840668