PureFection™ Transfection Reagent

Deliver more nucleic acids—plasmids, siRNAs, etc.—to cells than the leading lipid-based transfection reagent for effective and reproducible transfection
  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

Products

Catalog Number Description Size Price Quantity Add to Cart
LV750A-1 PureFection Transfection Reagent 1 mL $291.00
- +
Contact Us
LV750A-5 PureFection Transfection Reagent 5 mL $1,235.00
- +
Contact Us

Overview

Overview

Increase your transfection efficiencies

With SBI’s PureFection™ Transfection Reagent, you can deliver more nucleic acid—plasmids, siRNAs, etc.—than the leading lipid-based transfection reagent for effective, efficient, and reproducible transfections.

The easy-to-use protocol consists of a rapid, one-step, 15-minute incubation with the plasmid, small RNA, or other nucleic acid you’d like to transfect. Once the incubation is done, simply add directly to target cells—no media changes are required as PureFection works in the presence of antibiotics and serum.

The fast PureFection protocol makes it well-suited for high-throughput transfection projects.

  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

How It Works

How It Works

A fast and effective method for increasing transfection efficiencies

The PureFection Transfection Reagent uses a fast, 15-minute protocol

Supporting Data

Supporting Data

PureFection delivers higher transfection efficiencies than the leading lipid-based reagent

PureFection delivers higher transfection efficiencies than the leading lipid-based reagentPureFection delivers higher transfection efficiencies than the leading lipid-based reagent

Resources

Citations

  • Lin, KH, et al. (2021) MicroRNA-210 repression facilitates advanced glycation end-product (AGE)-induced cardiac mitochondrial dysfunction and apoptosis via JNK activation. Journal of cellular biochemistry. 1970 Jan 1;. PM ID: 34545968
  • Chuang, TD, et al. (2021) Long Noncoding RNA MIAT Modulates the Extracellular Matrix Deposition in Leiomyomas by Sponging MiR-29 Family. Endocrinology. 1970 Jan 1; 162(11). PM ID: 34491311
  • Lin, KH, et al. (2021) Diallyl Trisulfide Suppresses High-Glucose-Induced Cardiomyocyte Apoptosis by Targeting Reactive Oxygen Species-Mediated Hypoxia-Inducible Factor-1α/Insulin-like Growth Factor Binding Protein 3 Activation. Journal of agricultural and food chemistry. 1970 Jan 1;. PM ID: 34558885
  • Liang, J, et al. (2021) AKR1C3 and Its Transcription Factor HOXB4 Are Promising Diagnostic Biomarkers for Acute Myocardial Infarction. Frontiers in cardiovascular medicine. 1970 Jan 1; 8:694238. PM ID: 34568444
  • Yogeswaran, A, et al. (2021) The C0-C1f Region of Cardiac Myosin Binding Protein-C Induces Pro-Inflammatory Responses in Fibroblasts via TLR4 Signaling. Cells. 1970 Jan 1; 10(6). PM ID: 34073556
  • Nakayama, Y, et al. (2021) Txnip C247S mutation protects the heart against acute myocardial infarction. Journal of molecular and cellular cardiology. 1970 Jan 1;. PM ID: 33652022
  • Ho, CC, et al. (2020) Extracts of Jasminum sambac flowers fermented by Lactobacillus rhamnosus inhibit H2 O2 – and UVB-induced aging in human dermal fibroblasts. Environmental toxicology. 1970 Jan 1;:e23065. PM ID: 33270331
  • Liu, SP, et al. (2020) Tetramethylpyrazine reverses high-glucose induced hypoxic effects by negatively regulating HIF-1α induced BNIP3 expression to ameliorate H9c2 cardiomyoblast apoptosis. Nutr Metab (Lond). 1970 Jan 1; 17:12. PM ID: 32021640
  • Chuang, TD, Rehan, A & Khorram, O. (2020) Tranilast induces MiR-200c expression through blockade of RelA/p65 activity in leiomyoma smooth muscle cells. Fertil. Steril.. 1970 Jan 1;. PM ID: 32199621
  • Hsieh, DJ, et al. (2020) Diallyl Trisulfide (DATS) Suppresses AGE-Induced Cardiomyocyte Apoptosis by Targeting ROS-Mediated PKCδ Activation. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32283691
  • Chuang, TD, et al. (2020) Mechanism underlying increased cardiac extracellular matrix deposition in perinatal nicotine-exposed offspring. Am. J. Physiol. Heart Circ. Physiol.. 1970 Jan 1; 319(3):H651-H660. PM ID: 32795172
  • Chuang, TD, Rehan, A & Khorram, O. (2020) Functional role of the long noncoding RNA X-inactive specific transcript in leiomyoma pathogenesis. Fertil Steril. 1970 Jan 1;. PM ID: 33070965
  • Lai, CH, et al. (2019) β-catenin/LEF1/IGF-IIR Signaling Axis Galvanizes the Angiotensin-II- induced Cardiac Hypertrophy. International journal of molecular sciences. 1970 Jan 1; 20(17). PM ID: 31480672
  • Wen, SY, et al. (2019) Roles of p38α and p38β mitogen‑activated protein kinase isoforms in human malignant melanoma A375 cells. International journal of molecular medicine. 1970 Jan 1; 44(6):2123-2132. PM ID: 31661126
  • Chuang, TD & Khorram, O. (2019) Cross-talk between miR-29c and transforming growth factor-β3 is mediated by an epigenetic mechanism in leiomyoma. Fertility and sterility. 1970 Jan 1; 112(6):1180-1189. PM ID: 31843095
  • Broeckel, RM, et al. (2019) Vaccine-Induced Skewing of T Cell Responses Protects Against Chikungunya Virus Disease. Frontiers in immunology. 1970 Jan 1; 10:2563. PM ID: 31736977
  • Cheng, SY, et al. (2017) Lactate dehydrogenase downregulation mediates the inhibitory effect of diallyl trisulfide on proliferation, metastasis, and invasion in triple-negative breast cancer. Environ. Toxicol.. 2017 Apr 1; 32(4):1390-1398. PM ID: 27566995
  • Quan, K, et al. (2017) Icariside II induces cell cycle arrest and apoptosis in human glioblastoma cells through suppressing Akt activation and potentiating FOXO3a activity. Am J Transl Res. 2017 May 31; 9(5):2508-2519. PM ID: 28560001
  • Liao, PH, et al. (2017) Phosphorylation of cofilin-1 by ERK confers HDAC inhibitor resistance in hepatocellular carcinoma cells via decreased ROS-mediated mitochondria injury. Oncogene. 2017 Apr 6; 36(14):1978-1990. PM ID: 27748761
  • Huang, CY, et al. (2017) Mitochondrial ROS-induced ERK1/2 activation and HSF2-mediated AT1 R upregulation are required for doxorubicin-induced cardiotoxicity.. J. Cell. Physiol.. 2017 Mar 14;. PM ID: 28295305

Products

Catalog Number Description Size Price Quantity Add to Cart
LV750A-1 PureFection Transfection Reagent 1 mL $291.00
- +
Contact Us
LV750A-5 PureFection Transfection Reagent 5 mL $1,235.00
- +
Contact Us

Overview

Overview

Increase your transfection efficiencies

With SBI’s PureFection™ Transfection Reagent, you can deliver more nucleic acid—plasmids, siRNAs, etc.—than the leading lipid-based transfection reagent for effective, efficient, and reproducible transfections.

The easy-to-use protocol consists of a rapid, one-step, 15-minute incubation with the plasmid, small RNA, or other nucleic acid you’d like to transfect. Once the incubation is done, simply add directly to target cells—no media changes are required as PureFection works in the presence of antibiotics and serum.

The fast PureFection protocol makes it well-suited for high-throughput transfection projects.

  • Highly effective transfection technology—works with most cell types
  • Cost-effective alternative to lipid-based products
  • Nanoparticle-based gene delivery with low toxicity
  • Rapid 15-minute protocol makes PureFection ideal for high-throughput transfections
  • Works with both Plasmid DNA and siRNAs

How It Works

How It Works

A fast and effective method for increasing transfection efficiencies

The PureFection Transfection Reagent uses a fast, 15-minute protocol

Supporting Data

Supporting Data

PureFection delivers higher transfection efficiencies than the leading lipid-based reagent

PureFection delivers higher transfection efficiencies than the leading lipid-based reagentPureFection delivers higher transfection efficiencies than the leading lipid-based reagent

Citations

  • Lin, KH, et al. (2021) MicroRNA-210 repression facilitates advanced glycation end-product (AGE)-induced cardiac mitochondrial dysfunction and apoptosis via JNK activation. Journal of cellular biochemistry. 1970 Jan 1;. PM ID: 34545968
  • Chuang, TD, et al. (2021) Long Noncoding RNA MIAT Modulates the Extracellular Matrix Deposition in Leiomyomas by Sponging MiR-29 Family. Endocrinology. 1970 Jan 1; 162(11). PM ID: 34491311
  • Lin, KH, et al. (2021) Diallyl Trisulfide Suppresses High-Glucose-Induced Cardiomyocyte Apoptosis by Targeting Reactive Oxygen Species-Mediated Hypoxia-Inducible Factor-1α/Insulin-like Growth Factor Binding Protein 3 Activation. Journal of agricultural and food chemistry. 1970 Jan 1;. PM ID: 34558885
  • Liang, J, et al. (2021) AKR1C3 and Its Transcription Factor HOXB4 Are Promising Diagnostic Biomarkers for Acute Myocardial Infarction. Frontiers in cardiovascular medicine. 1970 Jan 1; 8:694238. PM ID: 34568444
  • Yogeswaran, A, et al. (2021) The C0-C1f Region of Cardiac Myosin Binding Protein-C Induces Pro-Inflammatory Responses in Fibroblasts via TLR4 Signaling. Cells. 1970 Jan 1; 10(6). PM ID: 34073556
  • Nakayama, Y, et al. (2021) Txnip C247S mutation protects the heart against acute myocardial infarction. Journal of molecular and cellular cardiology. 1970 Jan 1;. PM ID: 33652022
  • Ho, CC, et al. (2020) Extracts of Jasminum sambac flowers fermented by Lactobacillus rhamnosus inhibit H2 O2 – and UVB-induced aging in human dermal fibroblasts. Environmental toxicology. 1970 Jan 1;:e23065. PM ID: 33270331
  • Liu, SP, et al. (2020) Tetramethylpyrazine reverses high-glucose induced hypoxic effects by negatively regulating HIF-1α induced BNIP3 expression to ameliorate H9c2 cardiomyoblast apoptosis. Nutr Metab (Lond). 1970 Jan 1; 17:12. PM ID: 32021640
  • Chuang, TD, Rehan, A & Khorram, O. (2020) Tranilast induces MiR-200c expression through blockade of RelA/p65 activity in leiomyoma smooth muscle cells. Fertil. Steril.. 1970 Jan 1;. PM ID: 32199621
  • Hsieh, DJ, et al. (2020) Diallyl Trisulfide (DATS) Suppresses AGE-Induced Cardiomyocyte Apoptosis by Targeting ROS-Mediated PKCδ Activation. Int J Mol Sci. 1970 Jan 1; 21(7). PM ID: 32283691
  • Chuang, TD, et al. (2020) Mechanism underlying increased cardiac extracellular matrix deposition in perinatal nicotine-exposed offspring. Am. J. Physiol. Heart Circ. Physiol.. 1970 Jan 1; 319(3):H651-H660. PM ID: 32795172
  • Chuang, TD, Rehan, A & Khorram, O. (2020) Functional role of the long noncoding RNA X-inactive specific transcript in leiomyoma pathogenesis. Fertil Steril. 1970 Jan 1;. PM ID: 33070965
  • Lai, CH, et al. (2019) β-catenin/LEF1/IGF-IIR Signaling Axis Galvanizes the Angiotensin-II- induced Cardiac Hypertrophy. International journal of molecular sciences. 1970 Jan 1; 20(17). PM ID: 31480672
  • Wen, SY, et al. (2019) Roles of p38α and p38β mitogen‑activated protein kinase isoforms in human malignant melanoma A375 cells. International journal of molecular medicine. 1970 Jan 1; 44(6):2123-2132. PM ID: 31661126
  • Chuang, TD & Khorram, O. (2019) Cross-talk between miR-29c and transforming growth factor-β3 is mediated by an epigenetic mechanism in leiomyoma. Fertility and sterility. 1970 Jan 1; 112(6):1180-1189. PM ID: 31843095
  • Broeckel, RM, et al. (2019) Vaccine-Induced Skewing of T Cell Responses Protects Against Chikungunya Virus Disease. Frontiers in immunology. 1970 Jan 1; 10:2563. PM ID: 31736977
  • Cheng, SY, et al. (2017) Lactate dehydrogenase downregulation mediates the inhibitory effect of diallyl trisulfide on proliferation, metastasis, and invasion in triple-negative breast cancer. Environ. Toxicol.. 2017 Apr 1; 32(4):1390-1398. PM ID: 27566995
  • Quan, K, et al. (2017) Icariside II induces cell cycle arrest and apoptosis in human glioblastoma cells through suppressing Akt activation and potentiating FOXO3a activity. Am J Transl Res. 2017 May 31; 9(5):2508-2519. PM ID: 28560001
  • Liao, PH, et al. (2017) Phosphorylation of cofilin-1 by ERK confers HDAC inhibitor resistance in hepatocellular carcinoma cells via decreased ROS-mediated mitochondria injury. Oncogene. 2017 Apr 6; 36(14):1978-1990. PM ID: 27748761
  • Huang, CY, et al. (2017) Mitochondrial ROS-induced ERK1/2 activation and HSF2-mediated AT1 R upregulation are required for doxorubicin-induced cardiotoxicity.. J. Cell. Physiol.. 2017 Mar 14;. PM ID: 28295305